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Objective: To explore the effects of exercise and resveratrol on retinol binding protein 4( RBP4) mRNA and protein expression in visceral adipose tissue, plasma RBP4 concentration and blood glucose and insulin sensitivity of aged obese rats., Methods: To establish elderly obese SD rat model, 6 aged rats of natural growth were selected normal group, 24 obese rats were randomly divided into four groups: obesity control group, exercise group, resveratrol group, exercise combined with resveratrol group. For Resveratrol treatment, each rat was fed 52. 5 mg/( kg d), 5 times/week, continuous intervention for 8 weeks. After intervention, measured blood glucose, insulin sensitivity, visceral adipose tissue RBP4 mRNA expression( qRT-PCR), protein expression( Western blot) and plasma RBP4 concentration( ELISA)., Results: After 8week intervention, ( 1) RBP4 mRNA expression: The obesity group( 2. 63 ± 0. 45) was higher than that of the normal group( 2. 10 ± 0. 15)( P < 0. 05). The resveratrol group( 1. 84 ± 0. 33), exercise group( 1. 91 ± 0. 15), and exercise combined with resveratrol group( 2. 131 ± 0. 111) were lower than that of the obesity group( P < 0. 05, P < 0. 01). ( 2) RBP4 protein expression: The obesity group( 1. 346 ± 0. 025) was higher than that of the normal group( 1. 196 ± 0. 017)( P < 0. 01). The exercise group( 1. 025 ± 0. 006)was lower than that of the obesity group( P < 0. 01), The resveratrol group( 0. 735 ±0. 015) and exercise combined with resveratrol group( 0. 701 ± 0. 018) were lower than that of the exercise group( P < 0. 01). The exercise combined with resveratrol group was lower than that of resveratrol group( P < 0. 05). ( 3) Plasma RBP4 concentration: The obesity group [( 16. 00 ± 1. 54) μg/L]was higher than that of the normal group [( 13. 02± 2. 20) μg/L]( P < 0. 01). The exercise group [( 14. 76 ± 1. 56) μg/L] was lower than that of the obesity group, but the difference was not statistically significant. The resveratrol group [( 13. 59 ± 0. 07) μg/L]and exercise combined with resveratrol group[( 12. 98 ± 1. 69) μg/L] were lower than that of the obesity group( P < 0. 05, P <0. 01). ( 4) Blood glucose: The obesity group [( 17. 93 ± 6. 09) mmol/L]was higher than that of the normal group [( 11. 64 ± 3. 57) mmol/L ]( P < 0. 01). The exercise group [( 13. 36 ± 1. 82) mmol/L]was lower than that of the obesity group( P < 0. 05), the resveratrol group [( 15. 24 ± 2. 19) mmol/L] and exercise combined with resveratrol group [( 13. 95 ± 2. 26) mmol/L] were lower than that of the obesity group, but the difference was not statistically significant( P > 0. 05). ( 5) Insulin sensitivity: The obesity group( 0. 37 ± 0. 02) was lower than that of the normal group( 0. 39 ± 0. 02)( P < 0. 05). The resveratrol group( 0. 38 ± 0. 01) and the exercise group( 0. 39 ± 0. 02)were higher than that of the obesity group, but the difference was not statistically significant, the exercise combined with resveratrol group( 0. 39 ± 0. 15) was higher than that of the obesity group( P < 0. 05). Above indexes( except protein expression) were no significant difference between the each intervene groups( P > 0. 05)., Conclusion: RBP4 mRNA expression and protein expression in the visceral adipose tissue of aged obese rats, plasma RBP4 concentration and blood glucose were increased, and insulin sensitivity was decreased. Exercise and resveratrol could reduce the expression of RBP4 mRNA and protein expression and plasma RBP4 concentration. The simple exercise could significantly reduce the blood glucose and exercise combined with resveratrol could significantlyimprove insulin sensitivity. The decrease in protein expression, plasma RBP4 concentration and improve insulin sensitivity, exercise combined with resveratrol showed synergy.

Objective: To investigate effects of different intensity exercise prescription on visceral adipose tissue-derived serine protease inhibitor( Vaspin) mRNA and protein expression in visceral adipose tissue and plasma Vaspin concentration of elderly obese rats., Methods: SD rats as three stage of fattening divided the growth period, middle-aged and elderly, to establish rats model of elderly obesity, 6 natural growing aged rats were selected as blank control group, 18 elderly obese rats were randomly divided into 3 groups, 6 rats in each group: obese control group, low intensity exercise group, moderate intensity exercise group. Exercise prescription were 12 m /min × 15 min for each group, 4groups / times, rest between groups 5min, 60 min/time. 15 m/min×15 min for each group, 4 groups / times, rest between groups 5min, 60 min / time. Vaspin mRNA expression was measured by qRT-PCR and Vaspin protein expression was measured by WB in visceral adipose tissue and plasma Vaspin concentration was measured by ELISA after exercise., Results: After exercise intervention, (1) Vaspin mRNA(×103) expression:Obese control group(25. 761±11. 581) was higher than the control group(12. 161±5. 648). (P<0. 01), low intensity exercise group and moderate intensity exercise group((10. 482±2. 668), (13. 478±5. 115)) were lower than obese control group (P<0. 01), moderate intensity exercise group was higher than low intensity exercise group, but the difference was not statistically significant (P>0. 05). (2) Vaspin protein expression: Obese control group was higher than the control group (P<0. 01), low intensity exercise group was lower than obese control group (P<0. 05), but moderate intensity exercise group was higher than obese control group (P<0. 01). (3) Plasma Vaspin concentration: Obese control group((25. 000±3. 767) ng/mL) was higher than the control group((23. 765±3. 045) ng/m L), low intensity exercise group and moderate intensity exercise group((25. 637±5. 240) ng/mL and (26. 422±3. 4175) ng/mL) was higher than obese control group, but the difference were not statistically significant( P >0. 05). ( 4) Blood glucose and insulin sensitivity index: Blood glucose of obese control group was higher than the control group( P < 0. 01). Insulin sensitivity index of obese control group was lower than the control group, but the difference was not statistically significant( P > 0. 05). Blood glucose of low and moderate intensity exercise groups were lower than obese control group( P < 0. 05), but insulin sensitivity index of low and moderate intensity exercise groups were higher than obese control group( P < 0. 01)., Conclusion: Vaspin mRNA and protein expression in visceral adipose tissue and plasma Vaspin concentration of elderly obese rats are higher. Low intensity exercise can reduce Vaspin mRNA and protein expression, but increase exercise intensity may improve Vaspin mRNA and protein expression in visceral adipose tissue and plasma Vaspin concentration of elderly obese rats.

Objective: To exploer effects of exercise and resveratrol on visceral fat resistin expression and plasma resistin concentration in elderly obese rats., Methods: SD rats as three stage of fattening divided the growth period, middle-aged and elderly, toestablish rats model of elderly obesity. 6 natural growing aged rats were selected as blank control group( C). 24 elderly obese rats were randomly divided into 4 groups, 6 rats in each group: obese control group( OC), supplementary resveratrol group( OR), exercise group( OE), exercise combined with resveratrol group( OER). Running speed and time of animal were 15 m/min×15 min for each group, 4 groups/times, rest between groups5 min, 60min/time. For resveratrol treatment, each rat was fed 52. 5mg/kg, one time/day, 5 times/week, for 8 weeks. The content of blood glucose was measured, the insulin resistance was calculated, testing the expression of resistin mRNA in visceral adipose tissue and plasma Resistin concentration., Results: Body weight, visceral fat mass, body fat ratio, and blood glucose, and insulin resistance of OC group were higher than C group( P < 0. 01, P < 0. 05), Resistin mRNA×10~3 expression in visceral adipose tissue and plasma concentration of OC group(( 29. 80 ± 4. 91) μg/L and( 4. 30 ± 1. 93) μg/L, repectively) were higher than C group(( 23. 09 ± 6. 12) μg/L and( 3. 49 ± 0. 42) μg/L, repectively), but the difference was not statistically significant. Body weight, visceral fat mass, body fat rate, and blood glucose, and insulin resistance of OE group and OER group were lower than OC group( P < 0. 01, P < 0. 05), of OR group were lower than OC group, of OER group were lower than OE group, but the difference was not statistically significant. Resistin mRNA×10~3 expression in visceral adipose tissue of OR group( 21. 63 ±4. 91) was lower than OC group( P < 0. 05), of OE group and OER group(( 28. 21 ± 4. 80), ( 25. 22 ± 11. 12), respectively) were lower than OC group, but the difference was not statistically significant. Plasma resistin concentration of OR group and OE group and OER group(( 3. 71 ± 1. 01), ( 3. 29 ± 1. 19) and( 2. 99 ± 0. 44) μg/L, respectively), were lower than OC group, of OER group were lower than OE group, of OE group were lower than OR group, but the difference was not statistically significant., Conclusion: Elderly obesity result in insulin resistance and high blood glucose, resistin expression in visceral adipose tissueand and plasma resistin concentration is higher. In elderly obese rats, simple exercise and exercise combined with resveratrol can significantly reduce weight, visceral fat mass, body fat ratio, and blood glucose content, and insulin resistance, but that are less affected on resistin expression in visceral fat and plasma resistin concentration. Simple add resveratrol can reduce resistin mRNA expression in visceral adipose tissue.