Search

Objective To investigate the effect of diosmine on semen quality and sex hormone concentration in patients with spermatovenous ligation. Methods Clinical data were retrospectively collected from January 2019 to January 2022 on patients with varicocele admitted to Department of Urology, General Hospital of Southern Theater Command, divided into a control group and a study group based on whether they had a history of Diosmine use after surgery. Clinical data from both groups were matched using propensity score matching. Sperm quality and sex hormone tests were then compared before surgery, three months after surgery and six months after surgery. Results There were 73 in the control group and 73 in the study group. Both groups were balanced in terms of age, BMI, seminal fluid volume and testosterone (P > 0.05). Sperm motility, forward motility and testosterone were all increased 3 and 6 months after surgery, and sperm malformations, sperm DNA fragment index, follicle stimulating hormone and luteinizing hormone were all reduced significantly (P < 0.05 ). The results were also better than those in the control group for total sperm motility, forward motility, testosterone, sperm DNA fragment index, follicle stimulating hormone and luteinizing hormone (P < 0.05 ). Conclusions Diosmine can help to restore semen quality and improve sex hormone concentration in patients with varicocele after Laparoscopic varicocelectomy. [ABSTRACT FROM AUTHOR]

Objective: To develop a reversed-phase HPLC method for simultaneous determination of gastrodin, adenosin, 4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde in Rhizoma Gastrodia., Method: A Kromasil C18 column (4.6 mm x 250 mm, 5 microm) was used with a methanol-water-0.1% acetic acid gradient elution system. The eluates were detected at 270 nm, the flow rate was 1.0 mL x min(-1) and the column temperature was 35 degrees C., Result: The linear range of gastrodin, adenosin, 4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde were 19.1-383 (r = 0.999 9), 0.620-12.4 (r = 0.999 9), 2.45-49.0 (r = 0.999 9), 0.280-5.63 mg x L(-1) (r = 0.999 6), respectively. The average recoveries (n = 9) of the four components were 96.7% -97.7%, RSD < 1.6%., Conclusion: The method is accurate, sensitive and reliable for determination of gastrodin, adenosin, 4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde in Rhizoma Gastrodia.

A method for the isolation of sesquiterpenols from the Japanese Cedar, Cryptomeria japonica D. Don by preparative reversed-phase high performance liquid chromatography (RP-HPLC) was established. The preparation of the sesquiterpenols was carried out on a preparative liquid chromatograph with a YWC-Pack C18 column, and the mobile phase was isopropanol-methanol-hexane-water (50:35:10:5, v/v) at a flow rate of 5.0 mL/min. The two sesquiterpenols, (-)-cubebol and (+)-2,7(14),10-bisabolatrien-1-ol-4-one, were separated by liquid chromatography and identified by spectroscopic analyses (ultraviolet (UV), infrared (IR), nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS)). The purities of the two sesquiterpenols were 98.7% and 99.1%, respectively.