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In June 2011-June 2012, a fertilization experiment was conducted in a typical Castanea mollissima stand in Lin'an of Zhejiang Province, East China to study the effects of inorganic and organic fertilization on the soil CO2 flux and the relationships between the soil CO2 flux and environmental factors. Four treatments were installed, i. e. , no fertilization (CK), inorganic fertilization (IF), organic fertilization (OF), half organic plus half inorganic fertilization (OIF). The soil CO2 emission rate was determined by the method of static closed chamber/ GC technique, and the soil temperature, soil moisture content, and soil water-soluble organic carbon (WSOC) concentration were determined by routine methods. The soil CO2 emission exhibited a strong seasonal pattern, with the highest rate in July or August and the lowest rate in February. The annual accumulative soil CO2 emission in CK was 27. 7 t CO2 ·hm-2 ·a-1, and that in treatments IF, OF, and OIF was 29. 5%, 47. 0%, and 50. 7% higher than the CK, respectively. The soil WSOC concentration in treatment IF (105. 1 mg kg-1) was significantly higher than that in CK (76. 6 mg·kg-1), but was obviously lower than that in treatments OF (133. 0 mg·kg-1 ) and OIF (121. 2 mg·kg-1 ). The temperature sensitivity of respiration (Q10) in treatments CK, IF, OF, and OIF was 1. 47, 1. 75, 1. 49, and 1. 57, respectively. The soil CO2 emission rate had significant positive correlations with the soil temperature at the depth of 5 cm and the soil WSOC concentration, but no significant correlation with soil moisture content. The increase of the soil WSOC concentration caused by fertilization was probably one of the reasons for the increase of soil CO2 emission from the C. mollissima stand. [ABSTRACT FROM AUTHOR]

Objective: To investigate the mechanism of electroacupuncture (EA) in improving the long-term survival rate of mice after myocardial infarction by promoting angiogenesis and inhibiting ventricular remodeling., Methods: A total of 102 male C57BL/6 mice were randomly divided into sham operation, model and EA groups ( n =34 /group). The myocardial infarction model was established by permanent ligation of the anterior descending branch of the left coronary artery. Beginning from the 3rd day after ligation, EA (2 Hz/20 Hz) was applied to bilateral "Neiguan" (PC6) and "Ximen" (PC4) for 30 min, once a day for 28 days. The survival rate in 140 d was recorded and the left ventricular ejection fraction (EF) calculated by using echocardiography after the treatment. The left cardiac ventricular tissue was cut into sections to be stained with Masson's trichrome, wheat germ agglutinin (WGA) or α-smooth muscle actin (α-SMA) immunohistochemistry method, followed by measuring the collagen area in the marginal region of myocardial infarction and calculating the collagen volume fraction (for assessing the severity of myocardial fibrosis), measuring the sectional area of cardiomyocytes (for assessing the degree of myocardial hypertrophy), and ob-serving the newborn blood vessels and calculating the ratio of neovascularization area (for assessing the state of angiogenesis). The expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor (HIF)-1α protein in the boundary area of myocardial infarction were detected by Western blot., Results: After modeling, the survival rate, EF, and the thickness of the left cardiac ventricle were significantly decreased ( P <0.01), whereas the percentage of collagen deposition area, sectional area of cardiomyocyte, percentage of angiogenesis area, and the expression levels of VEGF and HIF-1α proteins were significantly increased ( P <0.01) in the model group relevant to the sham operation group. Compared with the model group, the survival rate, EF, the thickness of the left cardiac ventricle, the percentage of angiogenesis area, and the expression levels of VEGF and HIF-1α proteins were significantly increased ( P <0.05, P <0.01), while the percentage of collagen deposition area and the sectional area of the cardiomyocyte were considerably decreased in the EA group ( P <0.01, P <0.05)., Conclusion: EA of PC6 and PC4 can significantly improve the cardiac function and long-term survival rate in mice with myocardial infarction, which may be related to its functions in up-regulating the expression of HIF-1α and VEGF to promote angiogenesis and in inhibiting ventricular remodeling.

Objective: To investigate the effects of different acupoint combination on prevention and treatment of stress gastric ulcer and to search for a better acupoint combination., Methods: The theoretical basis and experimental research on treatment of stomach diseases with the combination of he-(sea) points and front-mu points as well as back-shu points and front-mu points are analyzed in this article., Results: The combination of he-sea points and front-mu points is suitable to be applied on acute diseases, febrile diseases and diseases of fu organs. While the combination of back-shu points and front-mu points is more applicable on chronic gastric diseases., Conclusion: It is proved that the combination of he-sea point (Zusanli ST 36) and front-mu point (Zhongwan CV 12) has reliable effect on prevention and treatment of stress gastric ulcer.

In June 2011-June 2012, a fertilization experiment was conducted in a typical Castanea mollissima stand in Lin' an of Zhejiang Province, East China to study the effects of inorganic and organic fertilization on the soil CO2 flux and the relationships between the soil CO2 flux and environmental factors. Four treatments were installed, i. e., no fertilization (CK), inorganic fertilization (IF), organic fertilization (OF), half organic plus half inorganic fertilization (OIF). The soil CO2 emission rate was determined by the method of static closed chamber/GC technique, and the soil temperature, soil moisture content, and soil water-soluble organic carbon (WSOC) concentration were determined by routine methods. The soil CO2 emission exhibited a strong seasonal pattern, with the highest rate in July or August and the lowest rate in February. The annual accumulative soil CO2 emission in CK was 27.7 t CO2 x hm(-2) x a(-1), and that in treatments IF, OF, and OIF was 29.5%, 47.0%, and 50.7% higher than the CK, respectively. The soil WSOC concentration in treatment IF (105.1 mg kg(-1)) was significantly higher than that in CK (76.6 mg x kg(-1)), but was obviously lower than that in treatments OF (133.0 mg x kg(-1)) and OIF (121.2 mg x kg(-1)). The temperature sensitivity of respiration (Q10) in treatments CK, IF, OF, and OIF was 1.47, 1.75, 1.49, and 1.57, respectively. The soil CO2 emission rate had significant positive correlations with the soil temperature at the depth of 5 cm and the soil WSOC concentration, but no significant correlation with soil moisture content. The increase of the soil WSOC concentration caused by fertilization was probably one of the reasons for the increase of soil CO2 emission from the C. mollissima stand.