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Ulcerative colitis (UC) is a kind of inflammatory bowel disease. Female patients with UC may face nutritional problems during pregnancy, therefore, the management of nutritional status and disease conditions is important to pregnant women with UC. For example, nausea and vomiting of pregnancy (NVP) lasting until the middle and late stages of pregnancy can further aggravate the difficulty of nutrition management during pregnancy in UC patients. In this paper, we report a case of nutritional management of a patient with UC and persistent NVP during pregnancy, with the aim of sharing our experience in dealing with complex nutritional problems during pregnancy.

Objective To identify the characteristics of nutritional problems in patients with Parkinsonism who participated in multi-disciplinary team (MDT) and to investigate the correlation between nutritional status and dysphagia in these patients. The predictive value of video fluoroscopic swallowing study (VFSS) and EAT-10 scale for malnutrition was compared. Methods Subjects were patients with Parkinsonism participated in joint MDT consultation project in Peking Union Medical College Hospital from November 2020 to January 2023. Subjective and objective dysphagia were evaluated by EAT-10 scale score and VFSS dysphagia score. Nutritional status of the patients was evaluated by geriatric nutrition risk index (GNRI), albumin, prealbumin, serum folic acid, vitamin B12 and temporal muscle thickness. Results A total of 30 participants met the criteria and were included in the study. The age was 45-82 (66.1±9.0) years old. Six (20%) were at risk of malnutrition shown b a comprehensive nutritional status assessment using GNRI. The areas under receiver operating characteristic(ROC) curves(AUC) of VFSS dysphagia score and EAT-10 score to predict malnutrition were 0.781 (0.568-0.995) and 0.927 (0.827-1.000), respectively. EAT-10 score was correlated with GNRI(r=-0.524, P＜0.01), BMI(r=-0.618, P＜0.001), prealbumin (r=-0.616, P＜0.001). The VFSS dysphagia score was only correlated with BMI (r=-0.446, P＜0.05) and prealbumin(r=-0.387, P＜0.05). Conclusions Patients with Parkinsonism requiring MDT often have multiple micronutrient imbalance. Patients′ subjective perception of dysphagia has a greater impact on their nutritional status than objective assessment of dysphagia.

Objective To evaluate the effect of oral probiotics containing 4 strains of lactobacillus on the vaginal microbiota and adjuvant treatment effect of patients with vaginitis during pregnancy. Methods A total of 395 cases were included, 171 cases in the control group received 7 days of antibiotic treatment, and 224 cases in the combined treatment group. After the combined use of antibiotics and probiotics for 7 days, they continued to take 1 probiotic pill daily for the 28th day. The patients received a vaginal microecological examination and clinical evaluation at study entry and 28 days to compare the vaginal lactobacillus between the two groups. Results There was a statistical difference in the proportion of vaginal lactobacillus and the efficacy of treatment between the two groups of patients (P＜0.05). The effective rates in the combined treatment group and the control group were 90.2% and 86.0% respectively, and the cure rates were 30.4% and 19.3% respectively. Conclusions Oral probiotics containing lactobacillus strains can increase the proportion of vaginal lactobacillus in patients with vaginitis during pregnancy which significantly facilitated the clinical treatment of vaginitis during pregnancy.

Strontium(Sr) is a trace element naturally found in the human skeletal system. In recent years, the potential impact of strontium on bone and cardiovascular health has called significant attention, particularly during pregnancy, when alterations in mineral metabolism may affect the health of both the mother and the fetus. This comprehensive review assesses the current understanding of the role of strontium as a nutritional substance during pregnancy, its effects on maternal health and fetal development, and its potential associations with pregnancy complications such as preeclampsia, gestational hypertension, and lactation-induced osteoporosis. The review also summarizes the possible effects of strontium deficiency, excess, and supplementation, and provides information for developing prenatal nutrition supplementation guideline.

Zinc deficiency during pregnancy is common and has been significantly associated with adverse pregnancy outcomes such as spontaneous preterm birth(PTB), recurrent abortion(RA), low birth weight(LBW), small-for-gestational-age infant(SGA), preeclampsia(PE), and gestational diabetes mellitus(GDM). Unfortunately, there is no specific biomarkers which are sensitive, easy-to-collect and detect available for the clinical evaluation of zinc nutritional status. However, recent studies have identified metallothionein and the ratio of oleic acid to dihydroxymethyl gamma-linolenic acid(LA/DGLA) as potentially candidates of biomarkers.This article focuses on summarizing and discussing the progress of domestic and international researches on zinc deficiency during pregnancy and adverse pregnancy outcomes, potential zinc biomarkers, and zinc deficiency treatments, aiming at providing ideas for perinatal nutritional guidance.

Mission planning of border control operations refers to the process in which the relevant forces of border control make use of the mission planning system to plan and design relevant actions in order to achieve a specific action destination, so as to form a relatively scientific and reasonable action plan. In this paper, strategic analysis is carried out from the subject object, system composition and hierarchy of border control action task planning, and based on the transmission algorithm, the basic process of border control action task planning is discussed.

Aiming at the problem that the vibration frequency of conventional vibration exciter is small and can not realize the resonance of high stiffness components, Based on the superharmonic vibration characteristics of nonlinear vibration system, designed a nonlinear ultraharmonic vibrator exciter composed of 3 linear springs and 3 nonlinear springs. Established the dynamic model of the nonlinear ultraharmonic vibrator exciter, solved the nonlinear vibration amplitude-frequency characteristic equation by using the multi-scale method, and analyzed the theoretical feasibility of the vibration exciter to achieve third ultraharmonic resonance. Analyzed effects of excitation force amplitude, nonlinear stiffness coefficient and nonlinear damping coefficient on nonlinear vibration amplitude-frequency response. Carried out dynamic simulation of nonlinear ultraharmonic vibrator exciter by using ADAMS, and carried out the vibration experiment of the nonlinear ultraharmonic vibrator exciter. The results show that under the adjustment of the nonlinear spring, the exciter can achieve third ultraharmonic resonance, which verifies the rationality of the design of the nonlinear super-resonant exciter.

Objective To discover novel indoleamine 2, 3-dioxygenase 1 (IDO1) inhibitors with new scaffold structures by screening ZINC and Chembridge databases using pharmacophore modeling and molecular docking. Methods We performed virtual screening of the ZINC database by molecular docking targeting the enzymatic active site of IDO1. The compounds with high scores were selected for enzyme activity test to find the new leads; A pharmacophore model was constructed based on 3 established IDO1 inhibitors that had been tested in clinical trials for virtual screening of the analogues of the lead compounds. The compounds matching the pharmacophore model were selected for inhibitory activity test, and the molecular dynamics was simulated to explore the binding mode of the compounds to IDO1. Results With molecular docking, we identified 11 lead compounds from more than 2 million virtual compounds and measured their enzyme activity. Among them, ZINC91657208 with a skeleton of 4-hydroxyquinoline was found to effectively inhibit the enzyme activity of IDO1 with an IC50 value of 77.15 μmol/L. Thirty-one analogues were obtained by substructure retrieval with 4-hydroxyquinoline skeleton. Ten compounds were selected by pharmacophore virtual screening and their inhibitory effect on the enzyme activity of IDO1 was tested. Three of the 10 compounds showed obvious inhibitory activities, and among them Chembridge29374490 had the lowest IC50 of 37.78 μmol/L, whose root mean square deviations (RMSD) of the skeleton were 1Å and 2.4Å after equilibrium by molecular dynamics simulation. Conclusion We identified new 4-hydroxyquinoline IDO1 inhibitors from ZINC and Chembridge databases.

With the 6-DOF manipulators of the multi-arm casting robot as the research object,the trajectory planning is designed by the 4-3-4 polynomial interpolation method in the joint space. Under the constraint of velocity,the breed algorithm is used to optimize the interpolation results to obtain the optimal motion time of manipulator. Contrast to the particle swarm optimization,the breed algorithm has the higher precision and more rapid speed in the convergence procedure. By means of the Matlab,the movement position,velocity and acceleration curve of each joint of the dual arm robot are obtained. The consequence shows this method can accurately realize the time optimal trajectory planning at the speed constraint.

Film mulching rice cultivation pattern can effectively relieve seasonal drought and chilling damage during rice production. Film mulching cultivation pattern could reduce the consumption of irrigation water and improve grain yield on the basis of increasing soil temperature. However, the application of film mulching increased production cost and environmental pollution was occurred by non-degradable mulch. Therefore, the biodegradable film and matching mulch machines were applied and studied, the development of film mulching cultivation was promoted. It can effectively control weeds, reduce the loss of fertilizer, increase its use efficiency, reduce the application quantity of pesticides and chemical fertilizer, and provide a new idea for the green high-yield and high-efficiency cultivation of rice. This paper systematically expounded the advantages and disadvantages of the existing film-mulching rice cultivation in rice production, and prospects the future development of rice film mulching cultivation. [ABSTRACT FROM AUTHOR]

Excessive application of nitrogen fertilizer in agricultural soils is considered as a main cause of ecological problems such as nitrogen leaching, which has become an important limitation to improving nitrogen use efficiency in agricultural production. Because of its physical, chemical, and biological stability, biochar when applied to soils can play a key role in nutrient cycling, potentially affecting nitrogen retention and improving soil fertility. However, experimental results were variable and dependent on the experimental set-up, soil properties, and fertilizer application rates and biochar were set up at the high and low levels, which possess a big blind area. A laboratory experiment was conducted to investigate the effects of biochar additions on dynamic changes of nitrogen under artificial rainfall conditions by adding 0, 0.5%, 2%, 4%, 6%, and 8% biochar (the biochar was prepared by pyrolyzing corn straw at 500-550℃ under oxygen limited condition) into soil columns collected from red dryland of southern China under urea application condition (300 kg/hm2). The results showed that the speed of leaching of NO3 - and total nitrogen was delayed by biochar applications. Compared with urea application treatment, the total nitrogen and NH4 + contents in leachate were significantly decreased by 4.02% and 8.93% when ≥2% biochar was applied into soil, and the NO3 - contents of leachate was obviously decreased by 3.23%-11.87% when the biochar ratio reached 4%. But there was no significant effect of adding small amount of biochar (such as 0.5%) on N leaching compared with urea application treatment. Leaching losses of NO3 - had been found to be highest because the NO3 - was mobile, and may not be taken up by plants. NO3 - leaching amount accounted for 84%-90% of the total nitrogen leaching amount, while NH4 + leaching only accounted for 0.4%-2%; The total nitrogen content of different soil layers in each treatment was similar regardless of the fertilization and biochar applications. While the total nitrogen contents in soils were markedly different among the treatments. Compared with urea application treatment, the content of soil total nitrogen was significantly increased by 4.62% to 10.62% when biochar addition was ≥2%, and the relationship between addition amounts of biochar (x) and the content of soil total nitrogen (y) followed an index equation: y=1.6e0.0845x (R2=0.99, n=24). The results above suggested that biochar could be used as a potential additive for nutrient retention in order to increase the utilization efficiency of chemical fertilizers. In conclusion, biochar additions could decrease the leaching amount of NH4 +, NO3 - and total nitrogen and partly increased the concentration of soil total nitrogen. However, the low biochar addition had no significant effects on reducing nitrogen leaching and increasing soil total nitrogen content. Therefore, the purpose to obviously decrease nitrogen leaching and increase the content of soil total nitrogen could be realized only when biochar addition was above 2% in dryland soil. Nonetheless, the effect of biochar addition on reducing soil nitrogen leaching needs to be further verified before biochar can be applied to agricultural fields on a large scale. [ABSTRACT FROM AUTHOR]

Overflow and lost circulation occurred to Nanpu 36-P3001 well in Jidong Oilfield during the 4th spud-in. The after-effect is very serious and the bottom hole circulating temperature is 150 t. The annular space is small after liner running. The well deviation is large and the flow resistance of annulus fluid column is large. To provide high-quality wellbore environment for under-balanced drilling for the 5th spud-in, high-performance high temperature cement slurry system and matching circulation fluid are selected according to the cementing difficulty of this well; the setup method of centralizer is simulated and optimized by software; and the auxiliary cementing technique and measures are improved to perform cementing construction safety, effectively improve the cementing quality and provide technical support for small annular space liner cementing of horizontal well of Jidong Oilfield. [ABSTRACT FROM AUTHOR]

Objective: To review the clinical features of a male twin affected with glutaric academia type I (GA-I) and analyze the variations of glutaryl-CoA dehydrogenase (GCDH) gene., Methods: Clinical data of the pair of twins and their parents were collected. Genomic DNA was extracted from peripheral blood samples, and variants of GCDH genes were detected by capture sequencing using a customized panel. Variants of the twins and their parents were verified by Sanger sequencing., Results: The level of glutaric acyl carnitine (C5DC + C6OH) was 3.26 μmol/L in the male twin. The relative level of glutaric acid in urine was 547.51 by gas chromatography mass spectrometry analysis. Cerebral ultrasonography showed that the patient had subependymal hemorrhage, but no serious clinical manifestation was noted. After treating with special formula milk powder and L-carnitine, the boy showed good growth and development. Two heterozygous variants of the GCDH gene were detected in the patient, among which c.416C>G was suspected to be pathogenic, while c.109_110delCA was unreported. The variants were respectively inherited from his parents. The twin girl only carried the c.416C>G variant., Conclusion: GA-I can be diagnosed by mass spectrometry, urine gas chromatographic mass spectrometry, imaging as well as genetic diagnosis. Early diagnosis and intervention is important.

A monolithic fiber of poly(butyl methacrylate-ethylene glycol dimethacrylate)[poly(BMA-EDMA)] was prepared using deep eutectic solvent (DES) of choline chloride-ethanediol as a porogen. The prepared fibers were applied to direct extraction of three polycyclic aromatic hydrocarbons (PAHs) in lake water samples by solid phase microextraction (SPME). The extracts were determined by ultra performance liquid chromatography (UPLC). The fiber without DES was prepared at the same time. Both fibers (with and without DES) were compared with a commercial polydimethylsiloxane (PDMS) fiber. The highest extraction efficiency was clearly obtained with poly(BMA-EDMA) fiber. The extraction time, extraction solvent, desorption time, desorption solvent, and ionic strength were optimized. Under the optimum conditions, the linear ranges for the three PAHs (naphthalene, biphenyl, and phenanthrene) were 0.1-6.0 mg/L ( r ≥ 0.9903). The limits of detection (LODs) of the three PAHs were in the range of 2.1-4.9 μg/L. The recoveries of the three PAHs spiked in lake water samples were 86.4%-111.3% with relative standard deviations (RSDs, n =6) of 11.2%-15.1%. This method is simple, stable, and inexpensive, and can be used for the determination of the PAHs in lake water samples.

A QuEChERS-liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of pyraclostrobin, thiophanate-methyl and its metabolite carbendazim in soil and citrus. The samples were extracted with methanol or acetonitrile, purified by primary secondary amine (PSA), then separated by LC, detected in multiple reaction monitoring (MRM) mass spectrometry mode via positive electrospray ionization. The analytes were quantified by matrix-matched standard solutions with external standard method. The limits of quantification (LOQs) of pyraclostrobin, thiophanate-methyl and carbendazim in different matrices were 5.8-7.0 μg/kg, 9.3-14.1 μg/kg and 2.1-2.6 μg/kg, respectively. For all the samples, the spiked recoveries ranged from 75.48% to 109.18%, and the relative standard deviations (RSDs) were 0.60%-5.11% ( n =5). The method is quick, easy, effective, sensitive and accurate. The matrix-matched calibration solutions can efficiently compensate matrix effects of the pyraclostrobin, thiophanate-methyl and carbendazim in LC-MS/MS analysis. The established method can be applied to the residue analysis of the real samples of soil, citrus peel, citrus pulp and citrus fruits.

Objective: To investigate the epigenetic regulation of neprilysin (NEP) gene in mouse neuroblastoma Neuro-2a (N2a) cells and further determine the interaction between DNA methylation and histone acetylation., Methods: N2a cells were treated with DNA methylation inhibitor, 5-aza-deoxycytidine (5-Aza-dc) at 3 and 5 μmol/L for 48 hours and histone deacetylase inhibitor, trichostatin A (TSA) at 300, 500 and 700 nmol/L for 24 hours. The expression of NEP after the treatment was evaluated by reverse transcription PCR(RT-PCR) and Western blotting. Bisulfite sequencing PCR (BSP) assay was utilized to detect the methylation status of NEP gene promoter regions. The level of acetylated histone H3 on NEP promoter was measured by chromatin immunoprecipitation (ChIP) assay., Results: 5-Aza-dc induced the demethylation of NEP gene and significantly increased NEP expression in a dose-dependent manner (P<0.01). TSA treatment significantly enhanced NEP expression by elevating the acetylation of histone H3 on NEP promoter (P<0.01, P<0.05). However, methylation status of NEP promoter was not altered by TSA treatment (P>0.05)., Conclusion: The expression of NEP gene is regulated by DNA methylation and histone acetylation in N2a cells. Histone acetylation has no effect on DNA methylation.

Objective: To investigate methylation status of Wif-1 and β-catenin expression in colorectal serrated lesions., Methods: Various colorectal lesions were collected including 52 cases of hyperplastic polyps, 41 cases of sessile serrated adenoma, 23 cases of traditional serrated adenoma, 24 cases of colorectal cancer and 24 cases of normal mucosa. All specimens were subject to immunohistochemical staining of β-catenin.SYBR Green PCR analysis of Wif-1 promoter methylation was performed in 29 cases of hyperplastic polyps, 29 cases of sessile serrated adenoma, 19 cases of traditional serrated adenoma, 14 cases of colorectal cancer and 16 cases of normal mucosa., Results: Abnormal expression rates of β-catenin in normal mucosa, hyperplastic polyps, sessile serrated adenoma, traditional serrated adenoma and colorectal cancer were 12.5% (3/24), 59.6% (31/52), 63.4% (26/41), 73.9% (17/23) and 100.0% (24/24), respectively. The corresponding methylation rates of Wif-1 promoter were 2/16, 10/29 (34.5%), 16/29 (55.2%), 15/19 and 13/14 (P < 0.05), respectively. Abnormal β-catenin expression was positively correlated with Wif-1 promoter methylation in traditional serrated adenomas (r = 0.536, P < 0.05)., Conclusions: Abnormal β-catenin expression and methylation rate of Wif-1 promoter are significantly higher in colorectal serrated lesions. Methylation of Wif-1 promoter may be related to the abnormal expression of β-catenin through activation of Wnt/β-catenin signaling pathway, which may contribute to the development of colorectal serrated lesions.

Background: It has been proven that ornithine aminotransferase (OAT) might play an important role in the oncogenesis and progression of numerous malignant tumors. The aim of this study is to detect the mRNA and protein expression of OAT in non-small cell lung cancer (NSCLC), as well as to analyze the bioinformatic features and binary interactions., Methods: OAT mRNA expression was detected in A549 and 16HBE cell lines by reverse transcription-polymerase chain reaction. OAT protein expression was determined in 55 cases of NSCLC and 17 cases of adjacent non-tumor lung tissues by immunohistochemical staining. The bioinformatic features and binary interactions of OAT were analyzed. Gene ontology annotation and signal pathway analysis were performed., Results: OAT mRNA expression in A549 cells was 2.85-fold lower than that in 16HBE cells. OAT protein expression was significantly higher in NSCLC tissues than that in adjacent non-tumor lung tissues. A significant difference of OAT protein expression was existed between squamous cell lung cancer and adenocarcinoma (P < 0.05), but was not correlated with the gender, age, lymph node metastasis, tumor size, and TNM stages. Bioinformatic analysis suggested that OAT was a highly homologous and stable protein located in the mitochondria. An aminotran-3 domain and several sites of phosphorylation, which may function in signal transduction, gene transcription, and molecular transit, were found. In the 54 selected binary interactions of OAT, TNF and TRAF6 play roles in the NF-κB pathway., Conclusions: OAT may play an important role in the oncogenesis and progression of NSCLC. Thus, OAT may be a novel biomarker for the diagnosis of NSCLC or a new target for its treatment.

Objective: To study the chemical constituents from the antioxidant fraction of Neo-Taraxacum siphonathum., Method: Various chromatographic techniques were used to isolate and purify the constituents. The structures were elucidated on the basis of chemical evidence and spectral analysis., Result: Ten compounds were isolated and identified from Neo-T. siphonathum, caffeic acid (1), chlorogenic acid (2), quercetin (3), luteolin (4), quercetin-3-O-beta-D-glucopyranoside (5), quercetin-3-O-alpha-D-arabinofuranoside (6), quercetin-3-O-alpha-D-arabinopyranoside (7), luteolin-7-O-beta-D-glucopyranoside (8), beta-sitosterol (9) and daucosterol (10)., Conclusion: Compounds 1-10 were isolated from this plant for the first time.

The full length cDNA of silkworm hibadh gene was cloned by RT-PCR and RACE (Rapid amplification of cDNA ends) technique. The hibadh gene and its deduced amino acid sequences were analyzed. The tissue distribution of hibadh gene in 5th instar silkworm larvae was tested by RT-PCR. The expression patterns of hibadh gene in simulated weightless environment were analyzed by real time RT-PCR. The results showed that the full length hibadh cDNA sequence was 1074 bp in lenth, including an open read frame of 969 bp encoding the entire coding region of Hibadh (GenBank accession No. EU719652). The deduced amino acid sequence similarities of hibadh between silkworm and Burkholderia ambifaria, Drosophila melanogaster, Apis mellifera, Xenopus tropicalis, Mus musculus, Homo sapiens were 46%, 43%, 48%, 44%, 45%, 45%, respectively. Signal peptide analysis showed that Hibadh was a secretory protein. There wasn't glycosyl-phosphatidyl inositol anchor site in Hibadh amino acid sequence. Molecular weight and isoelectric point of Hibadh were 34.1 kD and 9.14 respectively. The RT-PCR tests indicated that the hibadh gene expressed in head, silk gland, midgut, cuticle, blood, fat body, tuba malpighii of the 5th instar silkworm larvae. There were different expression patterns of hibadh gene during different silkworm embryo period in simulated weightless environment. Simulated weightlessness resulted in the expression of silkworm hibadh gene up regulated 2.3-fold (P < 0.05), up regulated 4.6-fold (P<0.01), down regulated 7.6-fold (P < 0.01), down regulated 2.6-fold (P < 0.05) during apophysis formation period, inverse period, trachea formation period, and whole embryo period, respectively. There was no significant change of hibadh gene expression during other period of silkworm embryo between simulated weightless and control groups. There were different response patterns to simulated weightless environment between hibadh gene and whole body of silkworm. Gene showed much higher sensitivity compared to whole body in response to environment. This study is useful for the further research on the gravity biological mechanism of hibadh gene.