Search

Objective To investigate the influence of smoking on subgingival flora after periodontitis treatment through microbiological experiments. Methods Fifty-nine patients with periodontitis who completed periodontal treatment in the Department of Stomatology of Nanjing Jiangbei Hospital from 2019 to 2021 were selected and divided into smoking group(30 patients) and non-smoking group(29 patients). The clinical samples of patients's periodontal pockets before and after treatment were collected for detection of Actinobacillus (Aa), Porphyromonas gingivalis (Pg), Plasmobacterium intermedius (PI), Bacteroides forsythiae (Bf), Fusobacterium nucleatum (Fn) and Streptococcus minimus (Pm). Results After treatment, the bleeding index and periodontal attachment of the smoking group were better than those before treatment, while the plaque area index, bleeding index, periodontal pocket depth and periodontal attachment of the non-smoking group were better than those before treatment, and the plaque area index, periodontal pocket depth and periodontal attachment of the non-smoking group were better than those of the smoking group, with statistically significant differences(P＜0.05). After treatment, the positive rate of Pg and Pi in the smoking group was lower than that before treatment, the positive rate of Aa, Pg, Pi, Bf, Fn and Pm in the non-smoking group was lower than that before treatment, and the positive rate of Pg, Bf, Fn and Pm in the non-smoking group was lower than that in the smoking group, with statistically significant differences(P＜0.05). Conclusion Compared with smokers, non-smokers have better periodontal attachment recovery and lower detection rate of periodontal bacteria after periodontal treatment.

Objective To compare the bacteriostatic effect of two disinfections on the surface of frequently touched objects in dental clinic, so as to provide the reference for proper disinfection. Methods Specimens from the control panel and surface of examination table of comprehensive treatment chair were taken for bacterial culture, record the bac⁃ teria content on the objects surface. Then disinfect the objects surface by using 500 mg/L chlorine⁃containing disinfec⁃ tant (routing group) and Gamma disinfecting wet wipes (test group) respectively, compare the qualified rate of bacterio⁃ stasis on object surfaces between two group. Results After 10⁃minute disinfection on surfaces, bacteriostatic rate of routing group and test group was(91.66±7.52)% and (93.87±6.12)% respectively, there was no significant difference be⁃ tween two groups (P>0.05). Conclusion The quaternary ammonium disinfectant for the dental clinic objects can reach the same effect as chlorine⁃containing disinfectant.

Objective: To calculate the cut-off values of speed of platelet recovery and its R-squared in patients with acute myeloid leukemia (AML) after initial induction chemotherapy, which were used to predict the complete remission (CR) of the first induction chemotherapy, and guide the clinic to choose the next appropriate chemotherapy regimen as soon as possible., Methods: A total of 117 patients with newly diagnosed AML in the Second Hospital of Shanxi Medical University were included. Patients were diagnosed by morphology, immunology, cytogenetics, and molecular biology (MICM) classification, and the risk stratification was evaluated in combination with the clinical situations of the patients at the time of admission. The peripheral platelet counts after the first induction chemotherapy were detected and the linear regression equation was used to calculate the recovery speed of platelet counts in 5 consecutive blood cell analysis before discharge. According to the ROC curve, the cut-off value between the recovery speed and the R-squared was calculated, and the cut-off value was used to divide the patients into different groups. The differences between groups were compared by Pearson χ 2 test to observe the remission effect of the first induction chemotherapy., Results: ROC curve analysis showed that the cut-off value for predicting the platelet recovery speed and its R-squared of the first induction chemotherapy to achieve remission was 4.059 5×10 9 /(L·d) and 72.7%, the sensitivity was 77% and 63.9%, the specificity was 62.5% and 67.9%, and the Youden index was 0.395 and 0.318, respectively. The patients were divided into different groups and compared according to the above cut-off values, and the results showed statistical differences ( P <0.001, P =0.001)., Conclusion: The cut-off value of platelet recovery speed and its R-squared after the first induction chemotherapy calculated by peripheral platelet count and ROC curve in AML patients can be used as an index to evaluate the remission. The faster the platelet recovery speed after chemotherapy is, the more likely patients achieve remission. The more stable the platelet recovery tendency is, the more likely patients achieve remission too.

In order to established a method for simultaneous determination of isoquercitrin, astragaline, quercetin and kaempferol in Lysimachia clethroides, the ionic liquid 1-hexyl-3-methylimidazolium hexafluorophosphate ([HMIM]PF6) methanol was used as the ultrasound-assisted extraction solvent combing with RP-HPLC. A Purospher star RP-C1 column was used with the mobile phase of aceto- nitrile, methanol and 0. 4% phosphate acid by gradient elution at the detection wavelength of 360 nm. The flow rate was 0.7 mL x min(-1), and the column temperature was the room temperature. Under the optimized conditions, the linear ranges were 2.54 x 10(-2)-2. 54, 2.50 x 10(-2)- 2.50, 1.54 x 10(-3)-0.154, 1.49 x 10(-3)-0.149 microg for isoquercitrin, astragaline, quercetin and kaempferol, respectively. The average recoveries of the four constituents were 101.1%, 98.90%, 101.0%, 101.6%, respectively. The method was green, simple, rapid and accurate, and provided a valid method for analysis of isoquercitrin, astragaline, quercetin and kaempferol in L. clethroides.

The heavy metals in the environment led to the defect of nutrient contents in grains and metabolic disturbance seriously. In order to research the effect of heavy metal copper and cadmium on nutrient contents and heavy metal contents of wheat grain, the present paper studied the nutrition element (P, K, Ca, Mg, Na, Fe, Mn, Zn and B) and contents of heavy metal (Pb, Cr, Hg, Cu and Cd) in organs of detached wheat spikes in vitro culture by ICP-MS/ICP-AES. The results showed that the weight of grain reduced significantly with copper and cadmium treatment. Under different copper treatment, the contents of P, K, Mg, Ca, Na, Mn and Zn increased, but Fe and B contents reduced. Exogenous cadmium promoted the absorption of P, K, Ca, Mg and Mn while interrupted the absorption of Na, Fe, Zn and B. Exogenous copper and cadmium treatment reduced the Hg content in wheat grain, but the Cu and Cd contents were 55-folds and 62 folds of the national food sanitation standard. All the data showed that the effects of copper and cadmium are disadvantageous to the accumulation of nutrient and heavy metal contents.

The gaseous photocatalytic degradation of dibenzofuran, dioxins-like substance, was investigated by using TiO2, Ce3+/TiOs and Fe3+/TiO2 as photocatalysts. The batch-type photocatalytic reaction experiments were conducted, and the effects of initial concentration of reactant, humidity, circulation flow rate and light intensity on the photocatalytic degradation of dibenzofuran were studied. And the models and methods for estimating photocatalytic reaction constants and Langmuir adsorption constants were proposed. Results showed that the photocatalytic activity of the TiO2 photocatalyst was obviously improved after doping with Fe3+ and Ce3+, and the photocatalytic activity of Fe3+/TiO2 was the best in three photocatalysts. The degradation rate of dibenzofuran increased with the increase of the initial concentration of gaseous dibenzofuran in the batch-type photocatalytic reaction system. The photocatalytic degradation of gaseous dibenzofuran was enhanced when proper quantities of water vapor existed. However, it would be inhibited if there was excessive water vapor. As the circulation flow rate and the light intensity increased, the photocatalytic degradation rate of gaseous dibenzofuran also increased. The photocatalytic reaction rate coefficients of dibenzofuran on the photocatalysts TiO2, Ce3+/TiO2 and Fe3+/TiO2 were 34.54 x 10(-5), 36.23 x 10(-5) and 37.95 x 10(-4) mg x (min x m2)(-1) respectively.