Search

Objective: To study the influence of early high calcium intake to adulthood obesity through detection the expression of uncoupling protein 2 ( UCP2) mRNA in muscle by a reverse transcription polymerase chain reaction ( RT-PCR) technique., Methods: 120 male Wistar rats were divided randomly into normal control group, high dose calcium group, medium dose calcium group and low doses calcium group, with basic diet and high calcium diet for 4 weeks. After intervention, blood fat was compared. Subsequently, all rats were fed basic diet for 3 weeks and blood fat was compared. Then, normal feed rats were randomly divided into normal controls and obesity induction group. Obesity induction group, high dose calcium group, medium dose calcium group and low doses calcium group were fed high-fat food. After 8 weeks, RT-PCR was used for analysis After obesity induction, weight the expression of UCP2 mRNA in muscle., Results: growth of three high calcium groups were below obesity induction group, weight growth of low doses and high doses of high calcium groups were no difference with normal control group. Serum triglyceride levels of high dose high calcium group were significantly lower than obesity induction group and no difference with normal control group. Expression level of UCP2 mRNA of obesity induction group and high doses of high calcium group were obviously lower than normal controls, low dose and medium dose high calcium group, medium dose high calcium group' expression was significantly higher than normal control group., Conclusion: In rats' early life high calcium intake can continue to affect adulthood obesity induced by high-fat feed, increase expression level of UCP2 mRNA, improve the disorder of blood fat metabolism.

Objective: To investigate the mechanism underlying the mechanism of odontogenic differentiation of bone marrow-derived mesenchymal stem cells (BMMSCs) induced by periodontal ligament stem cells (PDLSCs), and offer an experimental evidence for the combination of the two types of stem cells to make regenerative periodontal complex., Methods: By means of Transwell(R); chamber, PDLSCs and BMMSCs from miniature pigs were co-cultured indirectly at different mixing ratios of PDLSCs to BMMSCs, 10:1 (group A), 1:1 (group B), 1:10 (group C). On the other hand, PDLSCs and BMMSCs were respectively cultured alone as positive and negative control group. Fourteen days later, the expressions of scleraxis, matrix extracellular phosphoglycoprotein (MEPE), osteocalcin (OCN), osterix (OSX) were detected by immunofluorescence and real-time quantitative PCR (qRT-PCR) to determine the optimal ratio of PDLSCs to BMMSCs for odontogenic differentiation., Results: Immunofluorescence and qRT-PCR showed that the expression levels of sceleraxi, OCN and OSX protein and relative mRNA had no statistically significant difference in the A, B, C groups (P>0.05), but as for MEPE, its relative mRNA expression level in group A was significantly higher than that in group B or C (P<0.01)., Conclusion: In the indirect co-culture of PDLSCs and BMMSCs, BMMSCs can obtain PDLSCs' biological characteristics to different extent, and meanwhile, a small number of PDLSCs can also induce the odontogenic differentiation of BMMSCs.