1. [Synergistic Antitumor Effect of Amorphigenin Combined with Cisplatin in Human Lung Adenocarcinoma A549/DDP Cells].
- Author
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Zhong H, Zuo Y, Wu X, Peng Y, He H, Yang J, Guan C, and Xu Z
- Subjects
- A549 Cells, Adenocarcinoma genetics, Adenocarcinoma metabolism, Adenocarcinoma physiopathology, Adenocarcinoma of Lung, Apoptosis drug effects, Caspase 3 genetics, Caspase 3 metabolism, Cell Proliferation drug effects, Drug Synergism, Humans, Lung Neoplasms genetics, Lung Neoplasms metabolism, Lung Neoplasms physiopathology, Poly(ADP-ribose) Polymerases genetics, Poly(ADP-ribose) Polymerases metabolism, Rotenone pharmacology, Adenocarcinoma drug therapy, Antineoplastic Agents pharmacology, Cisplatin pharmacology, Lung Neoplasms drug therapy, Plant Extracts pharmacology, Rotenone analogs & derivatives
- Abstract
Background: Amorphigenin, a rotenoid compouns, from seeds of Amorpha fruticosa, has been shown to possess anti-proliferation activities in several cancer cells. To explore the antitumor effects of amorphigenin on cisplatin-resistant human lung adenocarcinoma A549/DDP cells and explore the underlying mechanisms., Methods: CCK-8 assay was used to measure the proliferation of A549/DDP cells; Colony formation assay was used to measure the colony formation of A549/DDP cells; Flow cytometry assay was used to detect the apoptosis rates; Western blot analysis was used to explore the expression of apoptosis-related proteins (caspase-3 protein, PARP protein) and lung resistance protein (LRP)., Results: Our results demonstrated that amorphigenin could inhibit the proliferation of A549/DDP cells with a inhibition concentration of 50% cell growth (IC50) at 48 h of (2.19±0.92) μmol/L. Amorphigenin could inhibit the colony formation ability and induce apoptosis of A549/DDP cells; Furthermore, amorphigenin combined with cisplatin showed synergistic proliferation-inhibitory effect and apoptosis-promoting effect in A549/DDP cells; reduced the expression of LRP of A549/DDP cells., Conclusions: Amorphigenin remarkably inhibits the proliferation and induces apoptosis in A549/DDP cells. Combination of amorphigenin with cisplatin had the synergistic inhibitory effect on A549/DDP cells by downregulating the expression of LRP. .
- Published
- 2016
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