1. [Effect of RAD18-siRNA on proliferation and chemotherapy sensitivity of human esophageal squamous cell carcinoma ECA-109 cells].
- Author
-
Lou P, Sun X, Zhou J, and Zou S
- Subjects
- Adjuvants, Pharmaceutic pharmacology, Cell Cycle, Cell Line, Tumor, Cisplatin pharmacology, Cyclin D1 drug effects, Cyclin D1 genetics, DNA-Binding Proteins administration & dosage, Down-Regulation drug effects, Down-Regulation genetics, Drug Resistance, Neoplasm drug effects, Drug Screening Assays, Antitumor methods, Drug Synergism, Esophageal Squamous Cell Carcinoma, Fluorouracil pharmacology, G1 Phase drug effects, G2 Phase drug effects, Humans, Metaphase drug effects, RNA, Small Interfering administration & dosage, Transfection, Ubiquitin-Protein Ligases administration & dosage, Carcinoma, Squamous Cell drug therapy, Carcinoma, Squamous Cell physiopathology, Cell Proliferation drug effects, DNA-Binding Proteins pharmacology, Esophageal Neoplasms drug therapy, Esophageal Neoplasms physiopathology, RNA, Small Interfering pharmacology, Ubiquitin-Protein Ligases pharmacology
- Abstract
Objective: To investigate the effect of RAD18-siRNA on cell proliferation and chemotherapy sensitivity of esophageal squamous cell carcinoma (ESCC) ECA-109 cells. Methods: RAD18-siRNA was transfected into human ECA-109 cells by Lipofectamine 3000. Quantitative PCR and Western blot were performed to detect RAD18 and CyclinD1 expression; CCK-8 assay was used to determine cell proliferation and chemotherapy drug sensitivity; flow cytometry was used to determine cell cycle. Correlation between RAD18 and CyclinD1 mRNA expression was analyzed by Pearson's correlation. Results: Compared with non-transfected cells, the expression of RAD18 in RAD18-siRNA group was significantly decreased ( P <0.05). The cell proliferation was inhibited ( P <0.05) and the cell number of G1 phase was increased, G2/M phase cells decreased ( P <0.05) in RAD18-siRNA group. After treatment with different concentrations of cisplatin or 5-FU, the survival rate of the two cell groups was reduced (all P <0.05), and the IC50 of RAD18-siRNA group was significantly lower than that of non-transfected group ( P <0.05). The mRNA expression of RAD18 was positively correlated with CyclinD1 expression in ESCC tissues( r =0.478, P <0.01). Conclusion: Down-regulated expression of RAD18 can decrease the cell proliferation and increase chemo-sensitivity of ESCC cells, and CyclinD1 may participate in the process.
- Published
- 2016
- Full Text
- View/download PDF