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With the rapid advancement of intelligent businesses, the pre-existing relationship between traditional network architectures and computing capabilities has made it difficult to meet the current demands, making the implementation of computing-network convergence inevitable. Under the new computing power network framework brought about by the convergence of computing networks, efficient and intelligent resource scheduling strategy has become a key link to improve user experience. However, the existing resource scheduling algorithms have a single optimization objective and cannot meet the differentiated business needs of multi-tenants. To this end, a Multi objective deep reinforcement learning resource scheduling (MODRLRS) was proposed to call the computing resources and network resources in the computing power network. The algorithm performs multi-objective scheduling optimization of computing network resources by constructing a Pareto optimal solution set to meet the personalized business needs of different tenants. Simulation experimental results show that compared with other multi-objective resource scheduling algorithms, the proposed algorithm improves the request acceptance rate by 4.9% and the compliant delay request rate by 4.78%, which can flexibly adapt to the unique requirements of various computing services.

In order to investigate the main factors affecting the effect of spray dust reduction on the particle size of coal dust after spray dust reduction, a quantitative mathematical model was obtained to characterize the quantitative relationship between the influencing factors and the particle size parameters of coal dust after spray dust reduction. Based on the comprehensive experimental platform for spray dust removal, orthogonal experiments and regression analysis methods were applied to study the change rule of coal dust characteristic particle size D90 after spray dust reduction under the effect of three factors, namely, spray frame distance from dust source, tunnel wind speed and nozzle water supply pressure, and correlation analysis was carried out on 25 groups of experimental results. The weight value of each influencing factor in the coal dust D90 after spray dust reduction was determined by the mean square variance decision making method. On this basis, combined with the weights of each influencing factor, a weighted-based multivariate nonlinear regression prediction model of coal dust D90 after spraying dust reduction was constructed, and its prediction results were compared and analyzed with those of the multivariate linear regression model. The results show that: the coal dust D90 after spray dust reduction decreases with the increase of the distance from the dust source to the spray frame, increases and then decreases with the increase of wind speed, and increases with the increase of water supply pressure, and there is a strong positive correlation between full dust, exhaled dust reduction efficiency and D90, the better the spray dust reduction effect, the larger the coal dust D90 after spray dust reduction; the weighting-based prediction model of coal dust D90 after dust spraying has higher accuracy and smaller error, and can accurately characterize the quantitative relationship between coal dust D90 after dust spraying and three influencing factors, namely, the distance of the spray frame from the dust source, the wind speed of the roadway, and the water supply pressure of the nozzle.

In order to optimize the dust control flow field structure of the fully mechanized excavation face in coal mine, improve the dust control efficiency of the local ventilation system, achieve energy conservation and emission reduction in the mine, and low-carbon prevention and control of dust disasters, numerical simulation was used to study the respiratory dust pollution of the fully mechanized excavation face under different pressure air jet volume and action height conditions. The results showed that the pressure air jet volume is the main factor affecting the range of respiratory dust pollution. As the volume increases, the time for dust particle aggregation to escape to the working face decreases, the dust particle aggregation area continuously moves backward, and the range of respiratory dust pollution increases; the pressure air jet action height is the main factor affecting the degree of respiratory dust pollution in the area where the excavation driver is located. As the height increases, the overall range of dust pollution slightly increases, and the average dust mass concentration in the area where the excavation driver is located shows a trend of first increasing and then decreasing. Based on this, the optimal pressurized air jet volume was determined to be 200 m3/min and the optimal action height was 2 m. After on-site application, the respiratory dust concentration at the excavation driver’s site decreased by 55.4%, and the respiratory dust mass concentration in the rear area of the excavation machine decreased by more than 73%.

In order to understand the mechanical response and failure characteristics of anchored fractured coal body under cyclic dynamic load, the dynamic load impact test of anchored fractured coal under cyclic impact dynamic load was carried out by using the split Hopkinson pressure bar test system. The effects of cyclic dynamic load times and dynamic load impact strength on the stress-strain curves, bolt axial force and macroscopic failure of anchored fractured coal were analyzed. The results show that with the increase of dynamic load strain rate, the strength of anchorage specimen increases, but with the increase of dynamic load impact times, the strength of anchorage specimen decreases. When the impact input energy is constant, with the increase of impact times, the internal cracks of coal gradually expand or even fail, and the limiting force of bolt on coal expansion decreases. Under high energy impact, the bolt cannot hinder the initiation and expansion of the internal cracks in the anchored fractured coal body, and the overall instantaneous failure of the anchored coal body is prone to occur.

The utilization of underground space is related to geological environment. Identifying the geological safety risk and their influencing factors of underground space utilization can provide scientific basis for underground space management and the safety of underground space facilities. Previous studies have utilized various methods including analytic hierarchy process (AHP), fuzzy mathematics, and neural network to analyze the geological environment conditions of urban underground space. However, their methods have limitations in terms of objective weighting. To address this, considering the data correlation, discreteness, and relativity (conflict), an improved analytic hierarchy process incorporating the criteria importance through intercriteria correlation (CRITIC) method was introduced. The CRITIC-Entropy combination weighting method, which made the weights more scientific and reasonable, was proposed to evaluate the geological safety risk of underground space utilization. In Xiongan New Area, the geological safety risks related to stress variation, bearing capacity, submergence and anti-floating, soil pressure change, and sand liquefaction were analyzed. The evaluation focused on four aspects: space, resources, environment, and disaster. Quantitative indicators, such as aquifer thickness, characteristic value of soil bearing capacity, compression modulus of soil, ground elevation, buried depth of groundwater level, land subsidence rate, and sand liquefaction index were selected to construct the geological safety risk evaluation index system for shallow (0−15 m), sub-shallow (15−30 m), sub-deep (30−50 m), and deep (50−100 m) underground spaces. The study reveals that the geological safety risk of underground space in the study area follows a pattern that deep layers have a lower risk compared to shallow layers. The areas with Ⅰ and Ⅱ risk grades are predominantly located at Baiyangdian and its surrounding areas, east of Nanzhang town, and east of Daying town. The cumulative acreage of Ⅰ and Ⅱ risk grades in shallow, sub-shallow, sub-deep, and deep underground space accounts for 54.49%, 42.51%, 41.06%, and 42.18%, respectively. Additionally, the dominant factors influencing the geological safety risk vary across different layers of underground space, while compression modulus of soil, characteristic value of soil bearing capacity, ground elevation, and buried depth of groundwater level show high weights. It is also important to consider the risk changes caused by the potential changes of land subsidence rate, buried depth of groundwater level, and different excavation ways in the future research. These findings provide a geological foundation for the scientific optimization of underground space utilization and disaster prevention and mitigation in Xiongan New Area and other similar areas.

Aiming at the complexity problem in the design of variable fractional delay filters, a design method was considered. Time-domain interpolation and Taylor series expansion were integrated to configure the coefficient of Farrow structure, and the corresponding filter related formula was derived. The designed filter had coefficient-dependence relation, nearly half of the filter coefficients were independent, and an implemented structure of low-complexity filter was obtained. Theoretical derivation and simulation verification were conducted from the aspects of complexity, magnitude response, group delay, and error. The analysis shows that the proposed method can effectively reduce the complexity and computational cost of variable fractional delay filters.

To enrich remote inversion methods of soil hydraulic parameters and achieve a fast and nondestructive prediction, 59 high-spectral in-situ soil samples in Xiangzhou District, Zhuhai City, were collected using Field Spec4 spectrometer. The remote hydraulic parameter models were established for two land types: grassland and bare land (saturated water content θs, residual water content θr, inverse of air entry value α, shape coefficient n, saturated hydraulic conductivity Ks, and soil moisture content θ). The results are as follows. ① The spectral curves exhibit clear linearity in three bands: 700–750 nm, 830–1 100 nm, and 1 520–1 620 nm, with the mean values of coefficient of determination for the linear fitting R2 all over 0.94. ② Among gradient boosting regression (GBR), partial least squares regression (PLSR), and random forest (RF), GBR performs the best and shows high sensitivity to the linear fitting parameter lga2 (the logarithm of the slope) in the 830-1100 nm band. It accurately predicts θs, α, n, and θ for grassland types (Relative Percent Deviation, RPD> 1.4). For the bare land types, the coefficient of determination RP² for all hydraulic characteristic parameters, except soil moisture content θ, is greater than 0.9, and RPD exceeds 2.0. ③ In most hydraulic characteristic models predicting the grassland types, the feature importance of the leaf area index (LAI) is the highest. Therefore, an effective inversion model is built based on field spectral data and LAI, with simple access to input parameters and good prediction effect, providing a potential for wide application in the future.

ObjectiveTo investigate the prevalence of overweight， obesity and fatty liver disease （FLD） in adult residents in a suburban area of Shanghai， and to explore the relationship between these conditions and eating out of home （EOH）.MethodsAdult residents from Zhongshan Street， Songjiang， Shanghai， were invited to report their frequency of EOH and undergo a physical examination using B-ultrasonography from May to September 2017. Binary logistic regression analyses were conducted to examine the relationship between EOH and conditions of overweight， obesity， and FLD.ResultsAmong the final sample size of 6 608 participants， the prevalence rates of overweight， obesity， and FLD were 41.65%， 14.71% and 38.29%， respectively. After adjusting for potential confounders， logistic analyses revealed that participants with an EOH frequency > once/week had a 1.44-fold higher prevalence of obesity （P=0.006）， a 1.35-fold higher prevalence of overweight/obesity （P=0.005）， and a 1.36-fold higher prevalence of FLD （P=0.008）.ConclusionThe prevalence of overweight， obesity， and FLD is relatively high in Songjiang， Shanghai. EOH may be a risk factor for these diseases， and residents are recommended to reduce the frequency of EOH.

Objective To investigate the correlation of peripheral blood lymphocyte, T-cell, Th-cell, and Ts-cell counts with the development of checkpoint-inhibitor-related pneumonitis in NSCLC. Methods The clinical data of 85 patients with NSCLC treated with immune checkpoint inhibitors (ICIs) were retrospectively analyzed.Paired t-test was used to analyze lymphocyte changes.ROC curves were utilized to analyze predictive performance.The Spearman correlation coefficient test was conducted to analyze the linear relationship between lymphocyte changes and CIP grade. Results A statistically significant decrease in lymphocyte, T-cell, Th-cell, and Ts-cell counts from the baseline was observed in patients at the onset of CIP (P < 0.05), whereas no such change was observed in the control group.ROC curve analysis revealed AUCs of 0.867, 0.843, 0.865, and 0.843 for lymphocyte, T-cell, Th-cell, and Ts-cell counts, respectively.A linear relationship was found between the percentage decrease in lymphocyte, T-cell, and Ts-cell counts from the baseline and the severity of CIP (P < 0.05). Conclusion Decreased lymphocyte, T-cell, Th-cell, and Ts-cell counts have a predictive value for the development of CIP, and the lymphocyte count change has the greatest predictive value.The percentage decrease in lymphocyte, T-cell, and Ts-cell counts from the baseline is correlated with the severity of CIP.

Pulmonary arterial hypertension is a clinical syndrome characterized by pulmonary vascular remodeling causing increased vascular resistance, which will lead to right heart failure and even death if left untreated. The pathogenesis of pulmonary arterial hypertension has not yet been clarified, and clinical treatments have not been effective in improving prognosis or reducing mortality. To investigate the pathogenesis of pulmonary arterial hypertension and to develop and evaluate more effective and safer drug treatments, establishing related animal disease models is very important. This paper outlines the pathological characteristics of pulmonary arterial hypertension and summarizes the various types of animal models of pulmonary arterial hypertension, as well as describes the progress of the application of these models in three therapeutic pathways and related drug research in the past five years, with a view to providing a reference for the selection of animal models of pulmonary arterial hypertension and research applications.

Aiming at the current problem of low grinding efficiency of domestic ball screw grinding, a new type of ball screw grinding device is proposed. On this basis, a four-factor three-level quadrature test is designed for the four main process parameters of grinding torque, grinding speed, grinding time and abrasive particle size in the grinding process of the lead screw; a set of optimal process parameters is obtained by taking the surface roughness of the lead screw as the detection index. Then the friction torque measurement test is carried out on the ball screw before and after grinding. The effect of grinding on friction torque performance of the ball screw is explored.

The establishment of prefecture-level cities is a major spatial achievement of the social structure transformation in the process of modernization in China. The optimization of the layout structure of such cities is an inherent requirement for deepening supply-side structural reform and is of great significance to the construction of a scientific and reasonable urbanization pattern. Using the district similarity coefficient and standard deviation ellipse model, this study discusses the spatial characteristics and existing problems of prefecture-level city setting. The results reveal the following: 1) In terms of spatial characteristics, since the reform and opening-up, the setting of prefecture-level cities in China has been greatly affected by policy factors, exhibiting a gradual trend of advancing from east to west and from coastal to inland. 2) After reform and opening-up, the addition of prefecture-level cities is an adjustment on the basis of the original regional system, including the establishment of a city, the merger of a city, promotion of a county or county-level city to a city, and so on. 3) With the development of economy and society, the "space" problem of the prefecture-level city setting has become increasingly prominent, which is closely related to the difference in the mode of city setting, resulting in the administrative centers above prefecture-level setting too close, "small horse and big cart" and other problems to varying degrees. 4) The spatial distribution of prefecture-level cities in China is generated in regional administrative districts against the background of industrialization and urbanization, developed in the strategic transformation of urban-rural differences and cross-border cooperation, and faced with the innovative challenge of regionalization adjustment in the process of regional restructuring and scale restructuring. 5) The "space" problem leads to the increase of the system cost of economic and social development to varying degrees. Therefore, the layout of productivity should be comprehensively considered in a wider scope, and the adjustment of prefecture-level administrative divisions can be carried out when necessary. The possible contribution of this study to the literature is reflected in the application of the similarity coefficient of administrative districts to reveal that "the prefecture-level city is highly similar to the original region." Moreover, the problems existing in the current setting are related to this, such as too close distance and call for great importance to the significance of the adjustment of administrative divisions of the prefecture-level city. The possible shortcomings are that the logic of such city administrative division adjustment needs to be further explored, and the analysis of the thinking path of this adjustment is insufficient. In the future, the research on relevant issues in prefecture-level cities should be strengthened. Additionally, it is necessary to change the thinking of the adjustment of the overall construction system and the internal adjustment of prefecture-level government districts, consider the layout of productive forces from a broader perspective, consider the level of administration, the scale of government districts and the spatial structure as a whole, and take comprehensive measures to meet the actual needs of national and local development.

In order to realize real-time and multi-dimensional control of substation risk early warning, a substation risk early warning and control system based on edge computing is designed. The sensing layer collects the operation data of substation equipment through the Internet of Things equipment, and transmits the data to the edge layer through the communication between edge equipment and external equipment. After receiving data, edge layer parses the data through the communication protocol to complete data consistency, and packages cloud services downstream data sending to the cloud computing layer. The layer uses cloud computing to compute and analyze substation equipment data and transmit it to the application layer. The application layer adopts support vector machine (SVM) algorithm to establish a risk early warning mechanism, divide the risk data attribute, and obtain warning value. Based on this, the risk situation corresponding to the early warning value is predicted by the logistic regression risk prediction model, and the probability of failure is obtained to complete the substation risk warning. Meanwhile, the early warning results are presented on the visual interface, and the corresponding risk warning is controlled and controlled by the control channel. The test results show that the communication jitter results of the system are all below 15 ms, which can realize the calculation of risk probability, obtain the number of various risk warnings of the substation, have good work energy efficiency, and realize the multi-dimensional risk warning and control of the substation.

In recent years, the incidence of extrahepatic cholangiocarcinoma (ECC) has been increasing annually. As a result of frequently invading adjacent structures, such as hepatic artery, hepatic vein, and portal vein, and low radical resection rate, the prognosis is poor. Even if radical resection is completed early, the 5-year survival rate is still less than 30%. At present, whether postoperative adjuvant therapy can improve the prognosis of ECC remains a research hotspot and a controversial point. This article will combine the latest research results to discuss the plan and status of postoperative adjuvant therapy after ECC, as well as analyze the effect of postoperative adjuvant therapy on ECC.

In order to improve the microstructure uniformity and wear resistance of iron-based cladding layer,the effects of Mo on the microstructure and properties of iron-based cladding layer were studied by X-ray diffraction (XRD),metallographic microscope,scanning electron microscope (SEM),microhardness tester,and wear tester.The results show that the cladding layer without Mo is mainly composed of austenite and acicular carbide (M7C3).The microstructure of the cladding layer is uneven,the overall hardness fluctuates greatly,the wear resistance is poor and there is a large scale peeling in the wear process;After adding Mo,the microstructure of the cladding layer is more uniform and refined,and the strengthening phases (M7C3 and Fe7Mo3) are mainly distributed in a network,the overall hardness is more uniform,and the wear resistance of the cladding layer is the best when the content of Mo is 10%,which is 1.76 times higher than that of the matrix.Therefore,adding Mo can improve the microstructure uniformity and wear resistance of iron-based cladding layer.The research results can provide theoretical and technical references for improving the surface wear resistance of materials.

Objective: To explore the epidemic spread and prevention strategy of COVID-19 by Omicron variant in a single office building. Methods: Retrospective data were collected for the period from March 19, 2022 to March 28, 2022. During this period, the infection rate, epidemic survey, transmission chain, population vaccination and Ct value of infected cases, as well as other data of 458 persons at risk in the single office building were collected. The response measures and outcomes were recorded, and the characteristics of this round of epidemic transmission were analyzed, and the prevention and control strategies were summarized. Results: The epidemic situation in a single office building lasted for 10 days from March 19 to March 28. Forty-two out of the 458 persons in the office building were infected, with an infection rate of 9.17%. After the large-scale single room isolation measure was put in place for key populations on March 21, the infection rate showed a significant downward trend, reversing the previous upward trend. The real-time reproduction number (Rt) value changed dynamically with time (from 10.8 to 2.2) and reached a value of lower than 1 on the third day of isolation. For patients with Ct value above 30, the positive conversion rate of close contacts was about 6.0%. For patients with Ct value between 20-30, the positive conversion rate of close contacts is higher, about 25.6%. Among patients with Ct value below 20, the conversion rate of close contacts was as high as 62.5%. Compared with CT value >30 and their corresponding close contacts as the reference group, the infection risk of close contacts of infected persons with CT value of 20-30 increased significantly (OR=5.37, 95% CI: 1.88-15.33). The infection risk of close contacts of infected persons with CT value

A standardization system is important for the high-quality and integrated development of producer services. However, the standardization system of producer services in China still requires improvement regarding standards coverage, effective supply, and internationalization. Therefore, establishing a standardization system that is scientifically regulated, technologically advanced, and forward-looking becomes urgent for the producer services. In this article, we study the development trend of producer services standardization, analyze the development status of producer services standardization in China and abroad, and summarizes the problems faced by the construction of a producer services standardization system in the new era. Moreover, the development goals, key directions, and implementation paths of the producer services standardization system for 2025 and 2035 are proposed. To provide technical support for standardizing the economic system of China, the standardization system of producer services should be improved to satisfy the demand for high-quality development, standards for new service formats should be timely formulated, connection of standards systems between producer services and other industries should be promoted, standardization personnel should be cultivated, and standards internationalization should be encouraged.

High-position geohazard chains are widely distributed along the Jiali fault zone, Tibet, China. They often induced severe flood disasters in the river basins of the Yigong Zangbu, Palong Zangbu, and the downstream of Yalu Zangbu, such as the famous Yigong, Guxiang, Jianmupuqu, and Midui high-position geohazard chains. Based on the field investigations and previously published data, these high-position geohazard chains are classified into 4 types: 1) high-position landslide-debris flow-dammed lake-flood, 2) high-position landslide-dammed lake-flood, 3) high-position debris flow-dammed lake-flood, 4) glacial lake outburst flood. The formation conditions of the high-position geohazard chains are analyzed from 4 aspects, that is 1) geological structures and earthquakes, 2) landscape and river system, 3) glacier, 4) meteorology. In the end, the main causes and development trends of these geohazard chains are discussed. We believe that the high-position geohazard chains would be more frequent along the Jiali fault zone, with the global warming and the enhancement of the human engineering activities.

Sedongpu gully is a branch gully on the left bank of the lower reaches of the Yarlung Zangbo River. It is located below the galabaire peak. It often accumulates abundant moraines and loose deposits every year. Affected by temperature, rainfall and earthquake, high-position geological disasters have occurred many times and caused the blockage of the Yarlung Zangbo River. Through field investigation, combined with Landsat and other multi-source and multi-phase remote sensing images and InSAR radar data, the development characteristics of high-position geological disasters in Sedongpu gully are analyzed, and the existing deformation bodies are interpreted and analyzed: There are many glaciers, collapses, landslides and ice lakes in sedongpu gully; In the past 20 years since 2001, there have been five high-position geological disasters blocking the mainstream of the Yarlung Zangbo River; There are 3 identified deformations and 2 suspected deformations in the upper part of the gully; According to the two high-position geological disasters occurred in 2018 in Sedongpu gully, it is considered that the provenance comes from one high-position rock avalanche area and three high-position ice avalanche areas. This paper provides preliminary data for the further study of high-position geological disasters in Sedongpu gully, and provides effective scientific ideas for the study of similar geological disasters.

To study the casing deformation (CD) in shale gas well fracturing caused by natural fracture slip, a fracture face stress model is built based on stress analysis, and a CD prediction model is established based on complex function to analyze factors affecting wellbore shear stress and CD. (1) The fracture and wellbore approach angles have significant impacts on the wellbore shear stress. In Weiyuan shale gas field, Sichuan Basin, under the common wellbore approach angle of nearly 90°, the wellbore is subjected to large shear stress and high risk of CD at the fracture approach angle range of 20° to 55° or its supplementary angle range. (2) When the fracture is partially opened, the wellbore shear stress is positively correlated with the fluid pressure, and negatively correlated with the fracture friction coefficient; when the fracture is fully opened, the wellbore shear stress is positively correlated with the natural fracture area. (3) The lower the elastic modulus and the longer the fracture length, the more serious the CD will be, and the Poisson's ratio has a weak influence on the CD. The deformation first increases and then decreases with the increase of fracture approach angle, and reaches the maximum when the fracture approach angle is 45°. (4) At a given fracture approach angle, appropriately adjusting the wellbore approach angle can avoid high shear stress acting on wellbore, and reasonable control of the fluid pressure in the fracture can reduce the CD risk. The shear stress acting on casing is usually much greater than the shear strength of casing, so increasing casing strength or cementing quality have limited effect on reducing the risk of CD. Caliper logging data has verified that the CD prediction model is reliable, so the model can be used to establish risk analysis chart and calculate deformation value, to provide a reference for quick CD risk prediction in fracturing design.

Operation energy consumption of flue gas desulfurization (FGD) increases greatly after finishing ultra-low emission retrofit, the average power consumption rate is 1.40%, which is equivalent to power supply coal consumption of about 4.4 g/(kW·h). With the increase of thermal power plants' operation pressure, ultra-low emission operation should consider both energy saving and pollution reducing. Taking 33 600 MW-level units using single-tower or double-tower FGD process as the research object, operation energy consumption was analyzed considering both power consumption rate and power consumption per unit of FGD. Meanwhile, the influencing factors of operation energy consumption were emphatically analyzed. Finally, outlook on operation energy saving work of FGD was proposed, considering measures including energy efficiency benchmarking management, operational optimization of FGD, energy-saving retrofit of key devices, quality control of slurry, detailed maintenance work and rational use of desulfurization additives.

To investigate the general laws of solute transport in homogeneous and heterogeneous soil,this study conducts column experiments in the laboratory to observe and monitor hydrodynamic dispersion under different conditions.The measured breakthrough curves of solute are fitted and analyzed by the classical advection-diffusion equation (ADE).The results reveal that the solute concentration has little effect on the hydrodynamic diffusion coefficient in homogeneous soil;however,the flow rate of solute transport can promote hydrodynamic diffusion,namely that a higher flow rate can lead to a larger hydrodynamic diffusion coefficient,and the breakthrough curves can be completed faster.There is a positive correlation between the hydrodynamic diffusion coefficient and the mean particle size of the sand at a certain flow rate.In addition,the scale effect is prominent in the solute transport process in soil;generally,the hydrodynamic diffusion coefficient increases with the traveling distance.Compared with the case of homogeneous soil,the heterogeneity and different layered structures of layered soil have a more significant influence on hydrodynamic dispersion.

This paper conducts the column experiments in the laboratory,simulates the removal of nitrogen and phosphorus in groundwater by natural zeolite and iron-oxide coated zeolite through the geochemical model PHREEQC,respectively,and predicts and compares the long-term removal performance of nitrogen and phosphorus by natural zeolite and iron-oxide coated zeolite.The results show that the iron-oxide coated zeolite is able to remove nitrogen and phosphorus more effectively than natural zeolite with coexistence of the two pollutants.The main mechanisms of nitrogen removal by iron-oxide coated zeolite are ion exchange and physical adsorption,while that of phosphate are hydration reaction of iron oxide.The simulation results also show that the removal of nitrogen and phosphorus by iron-oxide coated zeolite differs from each other a lot under different pH conditions and thicknesses of capping material.

For enhancing the corrosion resistance of the T6-aged high-strength Al alloys with higher strength, retrogression and reaging (RRA) treatments were used to optimize the morphologies, sizes, distribution of precipitates, especially grain boundary precipitates (GBPs). The effects of different retrogression treatments on the microstructures and mechanical properties were studied so as to gain suitable RRA process for 7B50 Al alloy plates. It is found that increasing the retrogression temperature or time will promote the coarsening of transgranular and intergranular precipitates in the center and surface layers of 7B50 Al alloy plates as well as the precipitation of stable η-MgZn2 phase, which will decrease the strength and raise the conductivity. The retrogression temperature will greatly affect the strength and conductivity. The continuously distributed GBPs induced by T6 aging become discontinuous after RRA treatment, accompanying with slightly increasing sizes of transgranular precipitates. Based on the strength and conductivity of the center and surface layers, 165℃/6 h is the suitable retrogression process for 7B50 Al alloy plates. However, the severe deformation of the surface grains compared to that of the central grains caused by hot rolling leads to a higher content of subgrains or substructures in the surficial grains, which promotes the surface layer to quickly reach the peak aging, and the subsequent retrogression treatment results in much more stable η phase in the surface layer. The formation of stable η phase as well as the coarsening or growth of transgranular precipitates could be mainly responsible for the strength difference between the surface and center layers. Although there are some differences about the grain structures between the surface and center layers after quenching/RRA treatments with some local subgrain growth, the positive impact of RRA treatment to the strength is apparently unable to compare with the obvious strength reduction caused by early precipitation of stable η phase in the surface layer. Thus, the RRA treatment cannot relieve the property difference between the center and surface layers of 7B50 Al alloy plates, but it can make the strength and conductivity of the center and surface layers to concurrently meet some working requirements.

The paper introduces Professor ZHUANG Lixing 's academic thought on Daoqi Tongjing (directing qi to preserve essence) needling technique and the clinical experience. Based on Huangdi Neijing ( the Yellow Emperor ' s Inner Classic ), Dongyuan needling technique and Professor JIN Rui 's Daoqi Tongjing needling method, and by inheriting the valuable experience from the ancient masters and associating with his own clinical practices, Professor ZHUANG Lixing fully displayes the characteristics of principles, methods, formulas, acupoints and techniques of acupuncture in diagnosis and treatment of diseases. He integrates the thought of regulating the mind with Daoqi Tongjing needling. This needling method focuses on directing qi through mind regulation and needle manipulation, in which, the operation steps are refined. Besides, this needling method involves both the reinforcing and reducing techniques. The satisfactory effect of this needling has been obtained in clinical treatment for many disorders such as qi reversion and disharmony of yin and yang .

Brain signals refer to electrical signals or metabolic changes that occur as a consequence of brain cell activity. Among the various non-invasive measurement methods, electroencephalogram (EEG) stands out as a widely employed technique, providing valuable insights into brain patterns. The deviations observed in EEG reading serve as indicators of abnormal brain activity, which is associated with neurological diseases. Brain‒computer interface (BCI) systems enable the direct extraction and transmission of information from the human brain, facilitating interaction with external devices. Notably, the emergence of artificial intelligence (AI) has had a profound impact on the enhancement of precision and accuracy in BCI technology, thereby broadening the scope of research in this field. AI techniques, encompassing machine learning (ML) and deep learning (DL) models, have demonstrated remarkable success in classifying and predicting various brain diseases. This comprehensive review investigates the application of AI in EEG-based brain disease diagnosis, highlighting advancements in AI algorithms.

Objective: To compare the efficacy and safety of eravacycline and polymyxin drugs in the treatment of multidrug-resistant complicated intra-abdominal infections(cIAI). Methods: The cIAI patients diagnosed or highly suspected of multidrug resistant bacterial infection who received eravacycline treatment at Sir Run Run Shaw Hospital and Shulan (Hangzhou) Hospital from June to August 2023 were prospectively included as the elacycline group. The cIAI patients with cIAI caused by multidrug resistant bacteria who received treatment with polymyxin (B or E) from January 2021 to August 2023 were retrospectively collected. The acute physiology and chronic health scores and age were matched with those of the elacrinid group, and they were included in the polymyxin group. The study mainly analyzed the clinical efficacy, microbial clearance rate, comprehensive efficacy, and safety of the two groups at the end of treatment and discharge. Results: A total of 72 patients with cIAI were included in the study, including 53 males and 19 females, aged (56.0±15.4) years. Among them, there were 28 cases in the eravacycline group and 44 cases in the polymyxin group. There was no statistically significant differences in the baseline, the proportion of patients who used combined drugs, the strains, the infection sites and infection conditions of patients between the two groups(all P >0.05).The clinical efficacy rate at the end of treatment [89.3% (25/28) vs 56.8%(25/44), P =0.004] and discharge [(88.5%(23/26) vs 63.6%,(28/44), P =0.024], microbial clearance rate at the end of treatment [(88.9% (24/27) vs 47.6%(20/42), P =0.001] and discharge [(91.3%(21/23) vs 59.5%(25/42), P =0.007], and overall recovery rate at the end of treatment [(89.3%(25/28) vs 52.3%(23/44), P =0.001] and discharge [(88.5%(23/26) vs 63.6%(28/44), P =0.024] in the erythromycin group were higher than those in the polymyxin drug group. In terms of safety, only 2 cases of mild vasculitis were observed in the eravacycline group. Conclusions: Combined regimens based on eravacycline showed better clinical effect in the treatment of cIAI caused by multidrug-resistant bacteria. The clinical effective rate, microbial clearance rate and comprehensive efficacy were significantly higher than those of polymyxin drugs at the end of treatment and discharge with good safety.

This paper introduces Professor ZHUANG Lixing 's experience in treating motor complications of Parkinson's disease (PD) with acupuncture combined with medication. Based on the characteristics of the alternation of "movement and stillness" in PD motor complications, Professor ZHUANG divides these complications into three distinct periods: "movement" stage, "stillness" stage and "alternation" stage, and proposes an integrated approach of acupuncture and medication, with staged treatment tailored to each period. The main acupoints include Jin's three needles to regulate spirit (four spirit needles, Shenting [GV 24], Yintang [GV 24 + ], Shenmen [HT 7], Sanyinjiao [SP 6]), along with hand tremor three needles (Hegu [LI 4], Quchi [LI 11], Dingchan), foot tremor three needles (Yinlingquan [SP 9], Yanglingquan [GB 34], Taichong [LR 3]), and Du 's three needles (Dazhui [GV 14], Jinsuo [GV 8], Mingmen [GV 4]). The primary medicinal formulas include Lingjiao Gouteng decoction, Banxia Baizhu Tianma decoction, Bazhen decoction combined with Buzhong Yiqi decoction, Sini decoction combined with Yougui pills, and Xiaochaihu decoction. This integrated approach effectively alleviates the motor symptom fluctuations in PD patients, helping them maintain a stable life.

Atrial fibrillation (AF) is the most prevalent sustained cardiac arrhythmia among humans, with its incidence increasing significantly with age. Despite the high frequency of AF in clinical practice, its etiology and management remain elusive. To develop effective treatment strategies, it is imperative to comprehend the underlying mechanisms of AF; therefore, the establishment of animal models of AF is vital to explore its pathogenesis. While spontaneous AF is rare in most animal species, several large animal models, particularly those of pigs, dogs, and horses, have proven as invaluable in recent years in advancing our knowledge of AF pathogenesis and developing novel therapeutic options. This review aims to provide a comprehensive discussion of various animal models of AF, with an emphasis on the unique features of each model and its utility in AF research and treatment. The data summarized in this review provide valuable insights into the mechanisms of AF and can be used to evaluate the efficacy and safety of novel therapeutic interventions.

Objective: To analyze the significance and factors influencing of CT scan under the modified Valsalva maneuver. Methods: Clinical data of 52 patients with hypopharyngeal carcinoma diagnosed from August 2021 to December 2022 were collected, all patients had calm breathing CT scan and modified Valsalva maneuver CT scan. Compare the exposure effect of the aryepiglottic fold, interarytenoid fold, postcricoid area, piriform fossa apex, posterior hypopharyngeal wall, and glottis with each CT scanning method. The effects of age, neck circumference, neck length, BMI, tumor site, and T stage on the exposure effect were analyzed. Results: In 52 patients, 50 patients（96.15%） completed CT scan at once time. The exposure effect of the CT scan under modified Valsalva maneuver in the aryepiglottic fold, interarytenoid fold, postcricoid area, piriform fossa apex, posterior hypopharyngeal wall was significantly better than CT scan under calm breathing（ Z =-4.002, -8.026, -8.349, -7.781, -8.608, all P <0.01）, while CT scan under modified Valsalva maneuver was significantly worse in glottis than CT scan under calm breathing（ Z =-3.625, P <0.01）. In the modified Valsalva CT scan, age had no obvious effect on the exposure effect. The exposure effect was better with long neck length, smaller neck circumference, smaller BMI and smaller T stage. The exposure of postcricoid carcinoma was better than pyriform sinus carcinoma and posterior hypopharyngeal wall carcinoma. But differences were not all statistically significant. Conclusion: The anatomical structure of the hypopharynx was clearly under CT scan with modified Valsalva maneuver, which clinical application is simple, but the effect of glottis was worse. The influence of age, neck circumference, neck length, BMI, and tumor T stage on the exposure effect still needs further investigation., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.)

Objective: To compare the strength difference between the interfacial screw and the interfacial screw combined with bone tunnel crossing technology to fix the tibial end of ligament during anterior cruciate ligament (ACL) reconstruction through the biomechanical test., Methods: Twenty fresh frozen pig tibia were randomly divided into two groups ( n =10) to prepare ACL reconstruction models. The graft tendons in the experimental group were fixed with interfacial screw combined with bone tunnel crossing technology, and the graft tendons in the control group were fixed with interfacial screw. The two groups of specimens were fixed in the high-frequency dynamic mechanics test system M-3000, and the length change (displacement), ultimate load, and stiffness of graft tendons were measured through the reciprocating test and load-failure test., Results: The results of reciprocating test showed that the displacement of the experimental group was (3.06±0.58) mm, and that of the control group was (2.82±0.46) mm, and there was no significant difference between the two groups ( t =0.641, P =0.529). The load-failure test results showed that the stiffness of the experimental group and the control group were (95.39±13.63) and (91.38±14.28) N/mm, respectively, with no significant difference ( t =1.021, P =0.321). The ultimate load of the experimental group was (743.15±173.96) N, which was significantly higher than that of the control group (574.70±74.43) N ( t =2.648, P =0.016)., Conclusion: In ACL reconstruction, the fixation strength of tibial end with interface screw combined with bone tunnel crossing technology is obviously better than that of interface screw alone.

Objective: Investigate the current situation of lung cancer cough diagnosis and treatment and the awareness of related issues among Chinese medical providers. Methods: Doctors, nurses, pharmacists from the oncology department, respiratory department, or general department were investigated using an electronic questionnaire from January 29 to March 29, 2021. There were 25 questions about lung cancer in the questionnaire. The questionnaire was including the most common accompanying symptoms in patients with lung cancer, the incidence of lung cancer-related cough, the assessment of the proportion of central antitussive drugs, and the commonly used central antitussives. Results: Questionnaires from 2 424 medical providers were collected from 402 hospitals in 21 provincial administrative units. Cough was the most common symptom in lung cancer. Most physicians believed that the incidence of lung cancer-related cough was 51%~75%, while the proportion of patients satisfied with the treatment was only 11%~20%. The evaluation of lung cancer-related cough was seriously insufficient. The leading cause of lung cancer-related cough was tumors. And the main problem was the inadequate antitussive effect of drugs in lung cancer-related cough management. The proportion of central antitussive medication usage in the secondary and tertiary hospitals was 93.9% and 92.0%, significantly higher than 75.0% in Primary hospitals (χ²=8.390, P =0.015). The proportion of the physicians who underhanded that codeine is at risk of addiction was 76.6% and 72.0% in the secondary and tertiary hospitals, which were significantly higher than 53.9% in Primary hospitals (χ²=9.240, P =0.010). In different occupations, the proportions of doctors and pharmacists who knew the risk of addicting to codeine were 73.0% and 82.6%, which were significantly higher than the 66.4% of nurses (χ²=21.200, P< 0.001). The Chinese medical providers were lack of training about the basic knowledge of using central antitussive medication. Conclusions: The proportion of patients who were satisfied with the effect of cough treatment is low. The medical staff did not have enough awareness of this. There was an urgent need to develop a consensus and standardize lung cancer cough diagnosis and treatment in China.

In this study, a comprehensive analytical method based on gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed for the determination of nine N -nitrosamines in animal derived foods. There are many kinds of N -nitrosamines in foods that are harmful to human health. However, the national standard GB 5009.26-2016 pertains only to the detection of N -dimethylnitrosamine; there are many drawbacks of this method, such as complicated sample preparation, low recovery rate, and poor reproducibility. Hence, it is of practical significance to establish a method for the simultaneous determination of a variety of N -nitrosamines. The optimal extraction conditions for the developed method were as follows: 10.0 g aliquots of the sample were placed in a 50 mL centrifuge tube, followed by the addition of 10 mL acetonitrile and 200 μL internal working standard solutions. After 30 min of freezing treatment, 4 g magnesium sulfate and 1 g sodium chloride were added for dehydration, and the tube was centrifuged at 9000 r/min for 5 min. After vortex centrifugation, 5 mL of the clear supernatant was purified using 150 mg polystyrene divinylbenzene (PLS-A). The purified extracts were dewatered using 1.6 g MgSO 4 and 0.4 g NaCl, and then filtered through a 0.22 μm membrane filter unit prior to GC-MS/MS analysis. Temperature-programmed was applied at an initial temperature of 50 ℃. After 0.16 min, the temperature was raised to 220 ℃ at the rate of 900 ℃/min for 5 min. N -Nitrosamines were separated on an HP-Innowax column (30 m×0.25 mm×0.25 μm). Identification and quantification were achieved using an electron impact ion (EI) source in positive ion mode with multiple reaction monitoring (MRM). The internal standard method was used to quantify the N -nitrosamines. Under the optimal conditions, the correlation coefficients of the standard calibration curves were not less than 0.99 in the range of 0.1-50.0 μg/L. The limits of detection were 0.03-0.30 μg/kg ( S/N =3), and limits of quantification were 0.15-1.00 μg/kg ( S/N =10). At spiked levels of 0.5, 1.0, and 3.0 μg/kg, the average recoveries of N -nitrosamines in spiked samples ranged from 80.4% to 98.5%, with relative standard deviations between 2.41% and 12.50%. This method was used to determine animal derived food products, except N -itrosomethylethylamine and N -nitrosomorpholine, others were founded. The results showed that N -nitrosamines levels in salted aquatic products were generally higher than those of the other samples. The method established in this study is simple to operate, and it does not require any time-consuming distillation extraction. Furthermore, there is minimal consumption of samples and reagents; consequently, the experiment cost is reduced, and the method is environmentally friendly. This method has theoretical and practical significance for the control of N -nitrosamines residues in animal derived foods, establishment of detection standards, and corresponding management measures.

Objective: To investigate the feasibility and effectiveness of a novel remnant-preserving anterior cruciate ligament (ACL) reconstruction with bidirectional barbed suture., Methods: Between February 2014 and January 2016, 96 patients (96 knees) with ACL injury who met the inclusion criteria were recruited and randomly divided into 2 groups ( n =48). All patients underwent ACL reconstruction with autologous tendon. The tibial remnant was fixed with PDS-Ⅱ suture (control group) and bidirectional barbed suture (trial group). There was no significant difference in age, gender, injury side, the interval from injury to operation, and preoperative knee stability (KT-1000 test), International Knee Documentation Committee (IKDC) score, and Lysholm score ( P >0.05). The knee stability (KT-1000 test), IKDC score, Lysholm score, proprioception, and Cyclops lesion after operation were recorded., Results: All incisions healed by first intention in both groups. Forty-four patients in control group were followed up 26-47 months (mean, 36.6 months), and 45 patients in trial group were followed up 26-48 months (mean, 35.6 months). At last follow-up, the IKDC score, Lysholm score, and KT-1000 test were significantly improved when compared with preoperative ones in both groups ( P <0.05); but no significant difference was found between 2 groups ( P >0.05). There was no significant difference in proprioception between 2 groups at 1 and 2 years after operation, and between 1 year after operation and 2 years after operation ( P >0.05) in the same group. The incidences of Cyclops lesion at 6 months, 1 year and 2 years after operation were 0, 4.44% (2/45), and 4.44% (2/45) in trial group, and 13.6% (6/44), 13.6% (6/44), 20.5% (9/44) in control group, showing significant differences between 2 groups at 6 months and at 2 years after operation ( P =0.012; P =0.022) and no significant difference at 1 year after operation ( P =0.157)., Conclusion: The remnant-preserving ACL reconstruction with bidirectional barbed suture can decrease the incidence of Cyclops lesions. However, this technique can not improve the effectiveness in terms of the clinical function and stability compared with the traditional technique.

The number of H7N9 bird flu cases was high and the situation was grim in guizhou province in 2017. To understand the molecular characteristics of the hemagglutinin gene (HA) and the risk of human infection with avian influenza virus A(H7N9) in Guizhou Province, 2017. Homology, genetic evolution and pivotal sites related to receptor binding regions, pathogenicity and potential glycosylation of 14 avian influenza viruses A(H7N9) were analyzed by a series of bioinformation softwares. It was cleared that there was 95.9%-100% similarity among 14 strains in nucleotide of the HA gene, and there were 96.8%-97.8% and 96.8%-97.9% similarities with vaccine strains A/Shanghai/2/2013 and A/Anhui/1/2013 recommended by WHO, respectively. Phylogenetic analysis showed that 14 HA genes were directly evolved in the Yangtze River Delta evolution branch, but they could be derived from five diffenrent strains. Then 13 of 14 strains cleavage site sequences of HA protein revealed they were low pathogenic avian influenza viruses, while A/Guizhou-Weining/CSY01/2017 was high pathogenic avian influenza virus. Mutation G186V at the receptor binding sites in the HA was found in all 14 strains, and mutation Q226L in 13 strains besides A/Guizhou-Weining/CSY01/2017. All five potential glycosylation motifs in the HA were conservative.

Objective: To understand the molecular characteristics of hemagglutinin (HA) and neuraminidase (NA) as well as the disease risk of influenza virus A H7N9 in Guizhou province. Methods: RNAs were extracted and sequenced from HA and NA genes of H7N9 virus strains obtained from 18 cases of human infection with H7N9 virus and 6 environmental swabs in Guizhou province during 2014-2017. Then the variation and the genetic evolution of the virus were analyzed by using a series of bioinformatics software package. Results: Homology analysis of HA and NA genes revealed that 2 strains detected during 2014-2015 shared 98.8%-99.2% and 99.2% similarities with vaccine strains A/Shanghai/2/2013 and A/Anhui/1/2013 recommended by WHO, respectively. Two strains detected in 2016 and 14 strains detected in 2017 shared 98.2%-99.3% and 97.6%-98.8% similarities with vaccine strain A/Hunan/02650/2016, respectively. Other 6 stains detected in 2017 shared 99.1%-99.4% and 98.9%-99.3% similarities with strain A/Guangdong/17SF003/2016, respectively. Phylogenetic analysis showed that all the strains were directly evolved in the Yangtze River Delta evolution branch, but they were derived from different small branch. PEVPKRKRTAR↓GLF was found in 6 of 24 strains cleavage site sequences of HA protein, indicating the characteristic of highly pathogenic avian influenza virus. Mutations A134V, G186V and Q226L at the receptor binding sites were found in the HA. All the strains had a stalk deletion of 5 amino acid residue "QISNT" in NA protein, and drug resistance mutation R294K occurred in strain A/Guizhou-Danzhai/18980/2017. In addition, potential glycosylation motifs mutations NCS42NCT were found in the NA of 9 of 24 strains. Conclusions: HA and NA genes of avian influenza A (H7N9) virus showed genetic divergence in Guizhou province during 2014-2017. The mutations of key sites might enhance the virulence of the virus, human beings are more susceptible to it. Hence, the risk of infection is increasing.

Objective: To establish a myelodysplastic syndrome transformed to leukemia cell line stably expressing green fluorescent protein (GFP), and to evaluate its biological characteristics and applications., Methods: SKM-1 cells were transfected by lentiviral particles with vector of GFP. The GFP positive single cell clone was isolated by limiting dilution and continued being cultured. The cells were injected into mice subcutaneously and were screened in vivo. Then SKM-1/GFP cells were obtained after tumour plaque was separated and cultivated. The cell morphology was observed by fluorescence microscopy. The GFP expression was further detected by flow cytometry. The cell proliferation was analysed by CCK-8 assay. SKM-1/GFP cells were inoculated to subcutaneous tissue of the immunodeficiency mice. The growth and invasion of the tumour were observed after tumour formation., Results: No differences in cell morphology and growth characteristics were observed between SKM-1 cells and SKM-1/GFP cells. The rate of GFP expression was 100%. No differences in cell proliferation were observed between SKM-1 cells and SKM-1/GFP cells. The tumour mass was observed after 14 days of subcutaneous vaccination in NOD/SCID mice. Spontaneous fluorescence from plaque was observed by living fluorescence microscopy at 30th day after vaccination. Homogenous GFP positive cells were observed by fluorescence microscopy in the frozen section of tumour mass. The invasion of SKM-1/GFP cells was also detected in heart, liver, stomach and kidney of mice., Conclusion: A myelody-splastic syndrome transformed to leukemia cell line stably expressing green fluorescent protein has been established successfully, which can track tumor cell sensitively and can be applied to the research of minimal residual leukemia. The establishment of SKM-1/GFP cells may serve as a powerful means for studing myelodysplastic syndrome transformation.

Objective To identify the effect and mechanism of recombinant platanus pollen allergen 2 (rPla a2) on the transcription and expression of orosomucoid 1-like 3 (ORMDL3) gene in NIH3T3 cells. Methods Quantitative real-time PCR and Western blot analysis were used to detect the influence of rPla a2 on the mRNA and protein levels of ORMDL3 and GATA3. After transfection of GATA3 siRNA or pcDNA-GATA3, the role of GATA3 was evaluated in rPla a2 regulating ORMDL3 expression. Dual-luciferase reporter assay was performed to measure the influence of rPla a2 and GATA3 on ORMDL3 promoter activity. Results The rPla a2 induced mRNA and protein expressions of ORMDL3 and GATA3. Knockdown of GATA3 inhibited the induction of rPla a2 upon ORMDL3. In addition, the induction effect was enhanced by over-expression of GATA3. ORMDL3 promoter activity was significantly promoted by rPla a2 and inhibited by knockdown of GATA3. Conclusion The rPla a2 up-regulates the expression of ORMDL3 mRNA and protein, which is mediated by GATA3 through targeting promoter region of ORMDL3.

Objective: To compare the clinical efficacy on vascular cognitive impairment with no dementia (VCIND) between the combined therapy of auricular acupuncture and acupuncture and the simple acupuncture., Methods: One hundred patients of VCIND were randomized into a combined therapy of auricular acupuncture and acupuncture group (a combined therapy group) and an acupuncture group, 50 cases in each one. The basic internal medicine treatment was applied in the two groups. Additionally, in the combined therapy group, auricular acupuncture and JIN 's three needling therapy were used. pizhixia (AT 4 ), xin (CO 15 ), shen (CO 10 ), gan (CO 12 ), erzhong (HX 1 ) were selected in auricular acupuncture, once every Monday, Wednesday and Friday; Niesanzhen , Zhisanzhen and Sishenzhen were selected in JIN 's three needling therapy, once a day. In the acupuncture group, JIN 's three needling therapy was just provided, once a day. The treatment was given for 4 weeks in the two groups. Montreal cognitive assessment (MoCA) and social function activities questionnaire (FAQ) were adopted for the evaluation comparison before treatment and in 2 weeks and 4 weeks after treatment in patients of the two groups., Results: Compared with those before treatment, the total scores of MoCA were improved in 2 and 4 weeks after treatment in the two groups (all P <0.01). The score in the combined therapy group was improved more apparently as compared with that in the acupuncture group ( P <0.01). FAQ score was reduced in the two groups (all P <0.05). The score in the combined therapy group was reduced more apparently as compared with that in the acupuncture group ( P <0.05). As compared with the result in 2 weeks of treatment, MoCA score was improved in the two groups in 4 weeks of treatment (both P <0.01), the improvements in the combined therapygroup were more obvious than those in the acupuncture group ( P <0.05) and FAQ score was reduced in the two groups ( P <0.05), but the difference was not significant between the two groups ( P >0.05)., Conclusions: The combined therapy of auricular acupuncture and acupuncture effectively improve the cognitive function and social function, which are better than the effects of simple acupuncture in VCIND. The improvement of the combined therapy in social function is more advantageous in the treatment of the first two weeks.

Objective: To investigate the effects of arsenic exposure before and during maternal pregnancy on heart development of fetal rats., Methods: According to body weight, thirty-two female SD rats (30 to 40 days of age) were randomly divided into control group, low dose group, middle dose group and high dose group with 8 rats per group. They were allowed free access to drinking water with 0, 37.5, 75 and 150 mg/L of sodium arsenite (NaAsO2) for 6 weeks, respectively. Then all the female rats and adult male SD rats were caged together for mating. Once female rats were determined to be pregnant, they would continue to drink deionized distilled water containing different concentrations of sodium arsenite for another 2 weeks. On embryonic day 16, rats were sacrificed to harvest fetuses. Female rats' weight changes, abortions, absorbed fetus number, growth and development of fetal rats were observed. Hematoxylin-eosin staining of serial cardiac slices was performed in embryos to observe cardiac morphology and structure. Fur arsenic contents of female rats were determined with the method of atomic fluorescence spectrometry., Results: Subchronic arsenic exposure caused slow weight growth in female rats. There were two cases of abortion in middle dose group and high dose group, respectively. Compared with these of control group, fetal and placental weight decreased (P < 0.05), and the incidence of fetal absorption increased (P < 0.05) in all arsenic-treated groups. Cardiac malformations in fetal rats including ventricular septal defect, atrial septal defect and tetralogy of Fallot were observed in low, middle and high dose group. The incidence of cardiac malformations increased with the increase of arsenic concentrations in drinking water. Compared with that of control group, the incidence of cardiac malformations remarkably increased in both middle and high dose groups (P < 0.05). Fur arsenic contents increased with the increase of arsenic concentrations in drinking water (P < 0.01)., Conclusion: Arsenic exposure before and during maternal pregnancy could cause abnormal cardiac development in fetal rats, and increased the risk of congenital heart disease.

Miscanthus sinensis Anderss is a perennial C4-grass. It is a promising bioenergy plant, which has been proposed as general feedstock for biomass and lignocellulosic biofuel production. In this study, the flower and leaf buds transcriptomes of Miscanthus sinensis Anderss were sequenced by the platform of Illumina HiSeq 2000. In total 98 326 Unigenes were generated by de novo assembly with an average length of 822 bp and N50 of 1 023 bp. Based on the NR, NT, Swiss-Prot, KEGG, GO and COG databases (Evalue < le-5), 74 134 (75.40%) Unigenes were annotated. A total of 45 507 Unigenes were mapped into different GO terms. In KEGG pathways identification, 36 710 sequences were assigned to 128 KEGG pathways. Sorghum bicolor (37 731, 60.86%), Zea mays (16 258, 26.22%), and Oryza sativa (3 065, 4.94%) showed high similarity to Miscanthus sinensis Anderss. And 24 photosynthesis-related enzyme genes were identified. The result provides a foundation for further characterizing the functional genes in Miscanthus sinensis Anderss.

Objective: To assess the perfusion changes in brains of patients with varying levels of generalized anxiety disorder (GAD)., Methods: A total of 38 GAD outpatients of Department of Psychiatry at our hospital from February to August, 2014 and 10 healthy controls received a 99mTC-ECD SPECT/CT scan with scenium analysis. Differences between brain perfusion and anxiety levels were analyzed by SPSS 16. 0 with one-way ANOVA, Pearson's Chi-square, t test and Spearman's correlation., Results: They were grouped according to the levels of anxiety severity, i. e. mild (n = 11) , moderate (n = 16) and severe (n = 11). They had significantly lower blood flow in right superior frontal medial gyrus, right precuneus, right putamen, bilateral paracentral lobule and bilateral supplementary motor area (t = -2.19, -2.14, -2.22, -2.34, -2.08, -3.26, -2.72, P < 0.05). Individuals had significantly greater blood flow in mild group than those of control group in right olfactory (t = 2.09 P = 0.05). Individuals of moderate group had significantly lower blood flow than those of control group in left superior frontal gyrus medial orbital, right superior frontal gyrus medial orbital and left supplementary motor area, but greater in right olfactory (t = -2.16, -2.24, -2.49, 2.17, P = 0.04, 0.04, 0.02, 0.04). Individuals had lower blood flow in severe group than those of control group in left frontal lobe, right putamen, left paracentral lobule, right paracentral lobule, left precuneus, right precuneus, left parietal lobe, left precentral, right precentral, right postcentral, left rolandic operculum, left supplementary motor area, right supplementary motor area and left central region (t = -2.32, -2.11, -3.16, -2.61, -2.39, -2.18, -2.32, -2.67, -2.14, -2.11, -2.25, -4.38, -3.54, -2.38, P = 0.03, 0.05, 0.01, 0.02, 0.03, 0.04, 0.03, 0.02, 0.05, 0.05, 0.04, 0.00, 0.00, 0.03). Statistical differences existed in right middle frontal gyms orbital part (mild: 0.96 ± 0.07, moderate: 1.03 ± 0.06, severe: 0.98 ± 0.08, P = 0.04) and left paracentral (mild: 0.91 ± 0.07, moderate: 093 ± 004 severe: 0.87 ± 0.07, P = 0.02). There was a tendency of negative correlation between perfusion in right middle cingulate and paracingulate gyri, left precuneus, right precuneus and left thalamus and anxiety scores by Spearman's correlation analysis (r = -0.28, -0.28, -0.27, -0.29, P = 0.09, 0.09, 0.10, 0.07)., Conclusion: Scenium software provides quantitative measurements for diagnosis of GAD in different anxiety levels. Also larger samples are required for confirming the results in further studies.

Objective: To assess the antibiotic resistance and molecular characterization of cholera strains and to provide basis for clinical treatment and prevention of cholera., Methods: 4 stains isolated from an outbreak of cholera epidemic in Huai'an City in Jiangsu province in September 2010 were characterized using antibiotic susceptibility, biotype analysis, virluence genes detection, ctxB gene sequencing, and PFGE analysis., Results: The 4 strains were all resistant to sulphamethoxazole/trimethoprim, erythromycin, streptomycin. High drug susceptibility of the samples was found to 6 kinds of antibiotics such as amikacin, norfloxacin, ciprofloxacin, gentamicin, chloramphenicol, ampicillin. The isolates expressed phenotypic traits of both serogroup O1 ogawa and El Tor and carried 9 kinds of virulence genes, ctxA, ace, zot, toxR, tcpI, ompU, rtxC, tcpA, and hlyA gene. They were also identified as harboring the classical ctxB genotype based on amino acid residue substitutions. The PFGE profiles of NotI showed a single banding pattern, while SfiI's was 2 banding patterns., Conclusion: The bacterium type of Vibrio cholerae causing the epidemic outbreak of cholera belonged to the atypical EL Tor variant which was also identified as toxicogenic strain. The mapping of the strains prompted that there should be the common contamination source. Drug sensitivity test can guide the clinical drug use, in order to reduce the emergence of resistant strains.

Objective: To investigate the mechanisms by which retinoic acid-induced gene G (RIG-G) protein regulates p21 gene expression., Methods: Western blot was used to detect the effects of RIG-G protein overexpression on p21 protein expression level in leukemia cell line NB4 cells and the phosphorylation of both c-Jun and JNK in U937 cells. The c-Jun expression plasmid and p21 gene promoter-containing reporter plasmid were co-transfected into 293T cells, to explore the regulatory effect of c-Jun protein on p21 gene expression by luciferase reporter assay., Results: Western blot showed that the overexpression of RIG-G protein significantly upregulated p21 protein level in the NB4 cells, and the level of p21 protein largely increased along with the induction of endogenous RIG-G protein during the differentiation of NB4 cells treated by all-trans retinoic acid (ATRA). Moreover, the phosphorylation of both c-Jun and JNK decreased in RIG-G-overexpressing U937 cells while total c-Jun and JNK proteins remained unchanged. After using the JNK inhibitor SP600125 to block JNK phosphorylation, the level of c-Jun phosphorylation was still dramatically reduced in the RIG-G-overexpressing U937T-RIG-G cells, compared with the control U937T-pTRE cells. These results indicated that the inhibitory effect of Rig-G protein on c-Jun phosphorylation could not only be through the JNK pathway, but also via some JNK-independent pathways. Luciferase reporter assay showed that when 0.1, 0.5, 1.0 and 2.0 µg c-Jun-expressing plasmids were respectively transfected into 293T cells, compared with the empty vector-transfected group, the relative luciferase activities were (83.0 ± 1.7)%, (73.7 ± 0.7)%, (68.9 ± 0.9)% and (64.1 ± 0.9)%, indicating that the transcriptional activity of p21 gene could be inhibited by c-Jun protein., Conclusions: RIG-G protein may suppress the phosphorylation of c-Jun protein through different signal pathways, thereby increasing the expression of p21 gene, arresting the cell cycle and inhibiting the cell growth in U937 cells.

The study of neuronal activity with low frequency has shown an increasing interest for its greater stability and reliability recent years. One challenge in analyzing this kind of activity is to find similarities and differences between signals efficiently and effectively. The traditional analysis methods, such as short-time Fourier transform, are easily obscured by background noises and often involve a large number of parameters. Therefore, this paper introduces a novel time-frequency analysis method based on wavelet transformation and half-ellipsoid modeling to extract instantaneous frequency and instantaneous phase information. This method overcomes some shortcomings of conventional time-frequency analysis. In this method, wavelet transformation is used to provide high-level representations of raw signals, and parsimonious half-ellipsoid models are used to extract changes in time domain and frequency domain of neural recordings. The method was validated to local field potentials (LFPs) of olfactory bulb of anesthetized rats during three different odor stimuli. The results suggested that this method could detect odor-relevant features from olfactory signals with large variability. The Odors then were classified with support vector machine (SVM) algorithm and the classification accuracy reached 79.4%.

This study investigated the effects of iron oxides with different crystallinities, ferrihydrite (SF) and hematite (SH), on the methane emission in paddy soil of South China under different water conditions, alternative drying/wetting cycles (DW) and continuous flooding (CF). The rates of methane emission, methane production potential and concentrations of Fe(II) and Fe(III) were determined. Results showed that compared with CF, the average methane emission rate was greatly inhibited by DW over 61%, and the addition of iron oxides reduced methane emission rates by 53%. Under the circumstance of DW and iron oxides addition, the combined inhibition effect on the average methane emission (65%-94%) and methane production potential (57%-93%) was significantly higher than the single effect caused by DW or iron oxides. Interestingly, DW coupling poorly crystalline ferrihydrite (SF-DW, 94%) posed a more significantly inhibitory effect on the methane emission than coupling crystalline hematite (SH-DW, 65%). Statistical analysis showed that there was a significantly negative correlation between the Fe(III) concentrations and the methane emission rates (R2 = -0.98, P < 0.01). Experimental results showed that DW facilitated the cyclic regeneration of Fe(III), and the Fe(III)/Fe(II) cycle in poorly crystalline ferrihydrite was more susceptible to DW than hematite. This study suggested that alternative drying/wetting cycles can remarkably enhance the inhibitory effect of iron oxides on methane emission from paddy soil, and the coupled suppression effect of DW and poorly crystalline ferrihydrite is more obvious than the coupled effect of DW and crystalline hematite.

Objective: To evaluate the prognosis value of plasma cystatin C in predicting adverse cardiac events after percutaneous coronary intervention (PCI) for non-ST-elevation acute coronary syndrome (NSTEACS)., Methods: A total of 277 patients (212 male, mean age 59 ± 12 years) with NSTEACS underwent successful PCI. The patients were then divided into MACE group and non-MACE group. Patients were divided into 4 groups according to the level of cystatinC : Q1 (<0.78 mg/L), Q2 (0.78-0.93 mg/L), Q3 (0.94-1.11 mg/L), and Q4 ( ≥ 1.12 mg/L) . Risk factors for MACE were analyzed by Cox regression analysis., Results: The plasma Cys-C level were higher in MACE group than in non-MACE group(P < 0.05). The areas under ROC curve of Cys-C, cTnI, hsCRP an CK-MB to predict cardiac event were 0.737,0.630,0.692 and 0.650 respectively. After a follow-up of 1 year, the MACE in the Q2, Q3, and Q4 groups was higher than in the Q1 group (Logrank = 23.751, P < 0.01). Multivariate Cox regression analysis showed that cystatin C elevation was an independent predictor of major adverse cardiac events (P < 0.01)., Conclusion: High plasma cystatin C concentration is an independent predictor of major adverse cardiac events in patients with NSTEACS treated with PCI.

Objective: To study the effect of Q-switched 1 064 nm Nd:YAG laser treatment on the proliferation of dermal collagen and expression of immunoglobulin binding protein/glucose related protein 78 (BiP/GRP78) in rats' skin and the mechanism of endoplasmic reticulum stress., Methods: Dorsal skin of 25 Wistar rats was divided into two parts equally after hair removal. Q-switched 1 064nm Nd:YAG laser was applied to treat rats' dorsal skin for 4 times at an interval of 2 days in the experiment part. The control part received no laser treatments. The rats' dorsal skin samples were taken on the 14th and 30th day after laser treatment to measure the dermis thickness and collagen bundles under HE stain and to measure the hydroxyproline content by alkaline hydrolysis method after laser treatment. The expression of BiP/GRP78 was also detected by immunohistochemical method. Statistics was used to analyze the data., Results: The dermis thickness increased by 29. 6% on the 14th day and 16.7% on the 30th day after laser treatment. The collagen bundles became thicker and denser. The hydroxyproline in the skin was also raised after laser treatment (P < 0.05). The immunohistochemical result showed the expression of BiP/GRP78 increased to 100% after laser treatment, showing a significant difference from the control group(X2 = 28.76, P < 0.01)., Conclusions: The Q-switched 1064 nm Nd:YAG laser treatment can induce endoplasmic reticulum stress, so as to enhance the protein folding and synthesizing precisely. The proliferation of dermis collagen is the base of effect of non-ablative skin rejuvenation.