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Objective: To explore the relationship between Helicobacter pylori (Hp) infection and asthma in children and its effect on immune function. Methods:95 children with asthma were randomly selected in our hospital from January 2013 to April 2016, defined as asthma group, and 100 healthy children were chose at the same time, defined as control group, then the status of Hp infection in two groups were tested. Venous blood of asthma group were collected, and compared the distribution situation of peripheral blood T lymphocyte subsets T helper cell 1 (Thl), T helper cell 2 (Th2), Th1/Th2, T helper cell 17 (Th17), Regulatory cells (Treg), Thl7/Treg and the serum cytokine content of interleukin 1-β(IL1-β), interleukin-2 (IL-2) and Interferon-γ (IFN-γ), interleukin-4(IL-4), interleukin-5 (IL-5), interleukin-17(IL-17), interleukin-25(IL-25) in children with Hp positive and Hp negative. Results: Hp infection rate was 36. 84％ in asthma group, which was higher than 16. 00％ in control group (P＜0.05);The Hp positive rate was 54. 10％ in asthma group at attacking period, which was higher than 5. 88％ in asthma group at remission period(P＜0.05). Th1 cells, Th1/Th2 ratio of Hp positive asthma children were significantly lower than Hp negative asthma children (P＜0.05), while the Th2 cells, Th17 cells, Th17/Treg ratio were significantly higher than Hp negative asthma children (P＜0.05). Contents of IL1-β, IL-4, IL-5, IL-17, IL-25 of Hp positive asthma children were significantly higher than Hp negative asthma children(P＜0.05), contents of and IL-2, IFN-γwere significantly lower than Hp negative asthma children (P＜0.05). Conclusion:Hp infection rate of children with asthma is higher than healthy children, and the Hp positive rate increase alone with the asthma progression, Hp infection can cause immune system dysfunction significantly, which lead to the occurrence or aggravate asthma, so attention should be paid in clinical work. [ABSTRACT FROM AUTHOR]

Objective To observe Helicobacter pylori (Hp) and CagA+-East Asian strains infection in different gastric mucosal lesions in Xi'an, and the influence of the infection on the expression of apoptosis proteins (Bax, Bcl-2, Fas and caspase-3) in different gastric mucosa lesions to elucidate the mechanism of gastric carcinogenesis related to Hp and CagA+-East Asian strains infection. Methods Gastric mucosal tissues were taken from patients with different gastric mucosal lesions in Xi'an. Confirmed by HE staining, including chronic superficial gastritis (CSG), chronic atrophic gastritis (CAG), chronic atrophic gastritis with dysplasia (Dys), and gastric cancer (GC). Hp infection and CagA+-East Asian strains infection were detected by fast urease test and immunohistochemical method. Expressions of Bax, Bcl-2, Fas and caspase-3 in each group were determined using immunohistochemical method. Results ① The infection rate of Hp and CagA+-East Asian strains was the highest in GC group and the lowest in CSG group (P<0.05). ② The expressions of Bax and Fas were significantly higher in Hp(+) group than in Hp(-) group (P<0.05). The expressions of Bax, Fas and caspase-3 were significantly higher in CagA+-East Asian Hp infection group than in non-CagA+-East Asian Hp infection group (P<0.05). However, Bcl-2 expression was lower than that in non-CagA+-East Asian Hp infection group (P<0.05). ③ The expressions of Bax, Fas and caspase-3 gradually decreased in CAG, Dys and GC groups, but Bcl-2 expression gradually increased. Conclusion Xi'an is an area with high Hp infection, and the majority of HP-infected persons carry East Asian CagA+ strains. East Asian CagA+ Hp infection can induce apoptosis of gastric mucosa epithelium and ultimately leads to gastric cancer when working with other factors. [ABSTRACT FROM AUTHOR]

Objective: To explore the possible angiogenesis mechanism of Huazhuo Jiedu Hewei Recipe (HJHR) in preventing and treating precancerous lesions of gastric cancer (PLGC)., Methods: Totally 66 Wistar rats were randomly divided into 6 groups, i.e., the normal control group, the model group, the retinoic acid (RA) group, the high dose HJHR group, the middle dose HJHR group, the low dose HJHR group, 11 in each group. PLGC model was duplicated by inserting a spring with Helicobacter. Corresponding medicines were administered to rats in each medicated group once daily by gastrogavage, 2 mL each time for 12 successive weeks. The effect of HJHR on hypoxia induced factor (HIF-1alpha) and vascular endothelial growth factor (VEGF) of PLGC in chronic atrophic gastritis (CAG) rats' gastric mucosa was observed by immunohistochemical assay and Western blot method., Results: Compared with the normal control group, the expression of VEGF and HIF-1alpha increased in the model group (P < 0.05). Compared with the model group, the expression of VEGF and HIF-1alpha decreased in each medicated group (P < 0.05). Besides, they were lower in the high and middle dose HJHR groups than in the RA group and the low dose HJHR group (P < 0. 05). There was no statistical difference between the low dose HJHR group and the RA group (P > 0.05)., Conclusion: HJHR could prevent and treat PLGC of CAG rats possibly through decreasing the expression of HIF-1alpha and VEGF in a dose-dependent manner.

Objective: To study the epidemiology, microbiology, pathogenicity, diagnosis and therapy of Helicobacter heilmannii (Hh) infection., Methods: Morphological methods, such as Gram staining of gastroscopic biopsy smears, Giemsa staining of histological slides and scanning electromicroscopy(SEM), and biochemistry techniques such as urease test(CLO), 14C urea breath test(UBT) were used to identify Hh infection in natural populations. The 16S rRNA gene of 4 strains of Hh were amplified, sequenced and analysed, using bacterial universal and Helicobacter genus-specific primers., Results: Twenty cases of Hh infection were found in 992 peoples with the positive rate of 2.0%. There existed the phenomena of intrafamily cluster of Hh infection. 14 case of the gastic tissues were CLO positive and 3 cases of these patients were UBT positive, the bacterium cannot be isolated in vitro. Sporadic invasion of lymphocyte/plasmic cell were found in gastric mucosa with Hh infection. Formation of lymph follicles and invasion of neutrophlic cell could be found too. The "old triple therapy" regimen could eradicate Hh easily. The homology of 16S rRNA gene among 4 strains was between 98.7%-99.7%, and can not be devided into two subtypes. The homology between Hh and H. felis or Hp were 95.6%-96.5% and 96.4%-97.1%, respectively., Conclusion: The prevalence of Hh in southern China is higher than that reported abroad. There may exist animal to person and person to person transmission mechanisms. Hh should be a new Helicobacter with lower activity of urease and weaker pathogenicity when compared with H. pylori. The infection of Hh is easy to eradicate through drug therapy.