Search

Objective: To investigate the therapeutic effect of L-Citrulline (L-Cit) on preeclampsia (PE) rat model and its effect on oxidative stress and trophoblast cell invasion. Methods: After establishing the PE rat model induced by N-nitro-L-arginine methyl ester (L-NAME), the rats were divided into Control group, PE group, PE+ low, middle and high dose L-Cit groups (L-L-Cit, M-L-Cit and H-L-Cit groups in sequence, with doses of 100, 200 and 500 mg/kg in sequence), n=6, continuous administration for 7 days. On the 21st day of pregnancy, systolic blood pressure (SBP), 24-hour urinary protein, serum superoxide dismutase (SOD) and malondialdehyde (MDA) were measured, and placental tissue was stained with hematoxylin-eosin (HE). The HTR-8/Svneo cells were divided into Control group, hypoxia/reoxygenation (H/R) group, H/R+L-Cit group and H/R+L-Cit+sc-221593 group. The cells were treated with H/R, respectively, and cultured with L-Cit (200 μg/mL) and the specific ERK1/2 inhibitor sc-221593 (10 μmol/L) for 48 h. Cell invasion was determined by Transwell assay. The protein expressions of total-ERK1/2, p-ERK1/2, total-p38, p-p38, matrix metalloproteinase (MMP)-2 and MMP-9 in placental tissues and cells were detected by Western blotting. Results: Compared with PE group, the SBP and 24 h urinary protein levels of the rats in L-L-Cit group, M-L-Cit group and H-L-Cit group were decreased (P<0.05); the placental tissue morphology was significantly improved; serum SOD was increased, while MDA was decreased (P<0.05); the phosphorylation levels of ERK1/2 and p38 and the protein expression levels of MMP-2 and MMP-9 in placental tissue were increased (P<0.05). Compared with H/R group, the number of invasive cells in H/R+L-Cit group increased (P<0.05); the phosphorylation levels of ERK1/2 and p38 and the protein expression levels of MMP-2 and MMP-9 increased (P<0.05). Compared with the H/R+L-Cit group, the above changes in the H/R+L-Cit+sc-221593 group were reversed (P<0.05). Conclusion: L-Cit may alleviate the symptoms of PE by reducing oxidative stress in PE and promoting trophoblast invasion by activating the ERK/JNK pathway. [ABSTRACT FROM AUTHOR]

Copyright of Xinjiang Agricultural Sciences is the property of Xinjiang Agricultural Sciences Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Arginine deiminase (ADI) was first high-efficient expressed in Corynebacterium crenatum SYPA 5-5. The ADI was purified by Ni-NTA affinity chromatography and SDS-PAGE analysis showed the molecular weight (MW) was 46.8 kDa. The optimal temperature and pH of ADI were 37 ℃ and 6.5 respectively. The Michaelis constant was 12.18 mmol/L and the maximum velocity was 0.36 μmol/(min·mL). Under optimal conditions, 300 g/L of arginine was transformed and the productivity reach 8 g/(L·h). The recombinant strain was cultivated in a 5-L fermentor and used for whole-cell transformation of 300 g/L arginine, under repeated-batch bioconversion, the cumulative production reached 1 900 g/L.