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Objectives. The objectives of the study were to develop a Czech version of the Cambridge depersonalization scale (CDS) and to examine its reliability, validity and discrimination quality as an instrument for screening depersonalization disorder. Methods. The CDS was translated from English into Czech and administered to 147 subjects along with Dissociation Experiences Scale (DES), Beck depression inventory II and Beck anxiety inventory. Thirteen subjects met ICD-10 criteria for depersonalization disorder, 69 for anxiety disorder and 65 were healthy controls. Statistical analysis. Cronbach's alpha and splithalf reliability was carried out to assess reliability. Spearman correlation analysis between CDS and DES depersonalization subscale and the Kruskal-Wallis test to compare scores of the CDS among the groups were carried out in order to examine criterion validity. Analysis of the receiver operating characteristics (ROC) was performed to examine discrimination quality of the CDS as an instrument for screening depersonalization disorder. Results. The Czech version of the CDS showed high internal consistency (Cronbach's alpha and split half reliability were both 0.95) and convergent validity (Spearman correlation with DES depersonalization subscale was 0.68). The scale was able to differentiate patients with ICD- 10 depersonalization disorder from the other groups. The area under the ROC curve was 0.975 and the best compromise between the true positive and false negative rate was at a cut-off point of 50, yielding a sensitivity of 1.00 and a specificity of 0.87 Conclusions. The Czech version of the CDS has good reliability, validity and discrimination quality, similar to the original instrument and can be used in both clinical research and practice. Study limitation. Results should be interpreted with caution given the relatively small sample size of the depersonalization disorder group. A larger sample in future study will be needed. [ABSTRACT FROM AUTHOR]