1. GENETICKÉ URČENÍ BARVY OČÍ U JEDINCŮ Z POHŘEBIŠTĚ ZNOJMO-HRADIŠTĚ.
- Author
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Lysáková, Petra, Brzobohatá, Kristýna, Drozdová, Eva, and Klíma, Bohuslav
- Subjects
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FOSSIL DNA , *DNA sequencing , *EYE color , *PHENOTYPES , *GENOTYPES , *HAIR dyeing & bleaching - Abstract
The identification of the individual phenotypic characteristics, most often the color of hair, eyes, and skin, applies both in forensic practice and in the research of ancient DNA. There are several types of approaches and protocols that can be applied according to the objectives and nature of the DNA to be investigated on the research sample. The main aim was the introduction of a method for the detection of rs12913832 polymorphism of the HERC2 gene, which is considered to be the strongest marker of the prediction of the eye color. Real-time PCR using a target-specific TaqMan® probe was used as it is the most suitable method for detecting polymorphism. This predesigned probe is characterized by a very short length of the sequence, making it ideal for research in ancient DNA. DNA sequences are often very short because of degradation and fragmentation. Allelic discrimination was chosen to detect the genotype. For the analysis, the skeleton material from the burial site Znojmo-Hradiště (Great Moravia Age) was used. From the whole set of 20 samples, 19 of them were successfully analyzed and genotyped. None of the detected genotypes was identified as the homozygous TT, which is characteristic for very dark shades of eyes. In contrast, the heterozygous assembly was detected in most samples. A genotype of TC, which is common in individuals with brown or hazel eyes, was determined in 15 samples. The light shade and the corresponding CC genotype were then determined in 4 individuals. [ABSTRACT FROM AUTHOR]
- Published
- 2018