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The descent and localization of eggs and embryos in individual segments of the reproductive tract of superovulated cows were studied in this work. For the induction of superovulation, serum gonadotropin (PMSG, Ivanovice in Haná) at a dose of 2,500-3,500 I.U. was used, in combination with 0.5 mg of Cloprostenol (Oestrophan, Spofa), administered 48 hours after gonadotropin treatment. The start of superovulation fell on days 9 to 12 of the sexual cycle and was conditioned by the presence of the corpus luteum (CL). After the onset of the heat, 2-3 inseminations were carried out using fresh semen. Donor cows were slaughtered 3, 4, 5, 6, and 7 days after the second insemination and isolated reproductive organs (Fig. 1) were divided into five segments (two on oviducts and three on uterine horns) by the applied ligature. In laboratory conditions superovulation response was determined accurately, the volume of ovaries was assessed according to water displacement and the segments of oviducts and uterus were rinsed with TCM 199 or PBS supplemented with FCS. 3, 4, 5, 6, and 7 days after insemination (Tab. I). 18.1 (+/- 3.55), 12.4 (+/- 0.91), 19.2 (+/- 2.86), 20 and 23 (+/- 2.44) CL on average were recorded, which corresponded to the ovulation of 64, 50, 56, 71 and 72 percent of stimulated follicles (Fig. 2). Within 3 to 7 days after insemination nearly triple enlargement of ovaries was also observed (Tab. I, Fig. 3). During the lavage of individual segments of the tubular reproductive tract, 38 per cent of eggs and embryos were detected in the uterus as early as 3 days after insemination (Tab. II). Unfertilized eggs and degenerated embryos were found in the 2nd and 3rd uterine segment, embryos at the stage of 8-16 blastomeres were localized in the 1st and 2nd segment of the uterus. Four days after insemination (Tab. III), about 64 per cent of eggs and embryos at the stage up to 16 blastomeres were found in the uterus, but embryos up to 32 blastomeres were still flushed out of the oviduct. On day 5 after insemination, 92 per cent of eggs and embryos were released into the uterus, being localized mostly in the cranial and medial part of the uterus (Tab. IV). 7.5 per cent of recovered eggs and embryos at the stage of early or compacted morulae were still detected in the oviducts.(ABSTRACT TRUNCATED AT 400 WORDS)

The results are analyzed of superovulation stimulation in the donors and the non-surgical embryo transfer in cattle. After superovulation induced by means of PMSG (Bioveta, Ivanovice in Haná) 6.2 and 6.3 ova and embryos were obtained, of which 66 to 70%, i. e. 4.2 and 4.3 embryos, respectively, were applicable in transfer. When the combination of PMSG with antigonadotropin (Antisergon, Bioveta, Ivanovice in Haná) was used, the embryos had somewhat better quality and their applicability was increased. After adjustment of the time of Antisergon administration the number of recovered ova and embryos was increased to 10.3 with 82.3% applicability (8.5 embryos per superovulation/donor). After super-ovulation stimulated with Folicotropin (Léciva, Praha) the yield of ova was 12.7, and 10.1 of the embryos (79.8%) were applicable. After the use of FSH-P (Burna-Biotec) 13.8 and 10.9 embryos were obtained per one superovulation. Non-surgical embryo transfer was performed in 1321 recipients over the last two years and a half. In 1984 the conception rate was 52.4%, in 1985 the average survival increased to 58.33% and in the first half of 1986 it reached 65.5%.