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The thesis deals with the design and subsequent construction of a special hermetic box for the treatment of biological substrates with the help of plasma discharge Gliding Arc. In the theoretical part of the thesis there is a summary of the basic information concerning the design of hermetic box, plasma, hexamethyldisiloxane as a precursor and wood as a lignocellulosic substrate. The experimental part of the thesis is focused on pilot measurements in which specimens were treated plasma and hexamethyldisiloxane. Furthermore, the effect of the treatment on the hydrophobic of the surface was investigated with the drop method. With the help of SEM microscopy was investigated the effect of modification on surface morphology