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A large part of the declining forest biodiversity (saproxylic insects, birds, fungi, bryophytes, lichens etc.) is linked to microhabitats that occur on structures typical of old-growth forests and are relatively rare in managed forests. In recent decades, alternative, more close-to- nature management practices have been slowly gaining ground, but their impact on microhabitats, and thus their potential value for forest biodiversity, has not yet been assessed. Therefore, I made a comparison of the occurrence and quality of microhabitats in 4 forest plots (8,3 to 42,5 ha) representing different forest management methods (traditional clearcutting management, alternative selective management and forest reservation at the time of data collection 65 years without intervention) in the natural conditions of the Central Bohemia. In the plots, using the adopted methodologies, I conducted an inventory of the presence of microhabitats on living trees and an inventory of deadwood and their assessment. The highest abundance and quality of microhabitats and deadwood were in the nature reserve, which at the same time completely exhausted the gamma diversity of all plots studied. The occurrence of microhabitats in clearcut and selective management forests was comparable and mostly low. The positive factors...