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Title: Metabolic response of indoor rock climbing and treadwall climbing Objectives: The goal of this work is to compare the differences between an indoor rock climbing and treadwall climbing in terms of the metabolic response of the organism. Methods: Forty-two climbers of three different performance levels participated in the research, who during seven days underwent in random order, separate measurements on an indoor wall and a climbing simulator at a speed of 4 m.min- 1 to a height of 19.5 meters with a slope of 90˚. The values of the metabolic response of the organism were monitored, specifically oxygen consumption (VO2), minute ventilation (VE), respiratory rate (DF), respiratory exchange ratio (RER), heart rate (HF), energy consumption (ES) and rating of perceived exertion (RPE). Results: Climbers of lower performance level were reaching average values of VO2, VE, DF, RER and ES higher than advanced climbers, both on the indoor wall and the an treadwall. In terms of average EC values, the indoor wall is more demanding for all groups (lower performance: treadwall 0.59 ± 0.07 kcal·kg-1 , indoor wall 0.69 ± 0.08 kcal·kg-1 ; intermediate: treadwall 0.57 ± 0.09 kcal·kg-1 , indoor wall 0.64 ± 0.11 kcal·kg-1 , advanced: treadwall 0.56 ± 0.08 kcal·kg-1 , indoor wall 0.62 ± 0.06 kcal·kg-1 )....

Breathing is essential part of our life. Breathing exercise can help acquirement of proper breathing. Thanks to this process, sports performance can be improved. The aim of our work is to determine differences in values and compare results of spiroergometric parameters from testing on a bicycle ergometer in the laboratory of functional load diagnostics. We obtained the results by an entrance test and a final test tested after eight weeks. Between testing, selected individuals performed a respiratory intervention program. Nine performance university athletes, especially focused on football, got involved in our research. Data at the rest position and load were crucial. We focused on dynamic ventilation parameters such as tidal volume, respiratory rate, minute ventilation or oxygen consumption. In the output test, we also recorded changes in values in the respiratory segments, abdominal, thoracic and subclavian areas. We verified the results from the point of view of material and statistical significances. Both of these significances were evaluated in this thesis. The materially and statistically significant differences in the results of measurement were improved in the oxygen consumption by our probands. The studied probands were able to improve in various phases of load, for example, the material significance was detected at the respiratory rate during the load of three watts per kilogram. Ascertained results were not improved in all monitored parameters. The most significant changes were discovered in the three-watt load during the respiratory rate from 32,8 +- 7,2 to 30,9 +- 5,1 breaths per minute. Probands reduced oxygen consumption by 14.8 percent.

Title: Comparison of internal loading on the organism by double poling and diagonal striding during the sprint on the roller ski Objectives: The aim of the diploma thesis is to compare the internal loading using the Cortex Metamax 3B and the SenzoStar GL devices in a two-hundred meters long section performed by the maximum effort between the double poling and diagonal striding about the 10.4% climb on roller skis. Methods: The diploma thesis is conceived as an empirical field research, the aim of which is to compare a double poling and diagonal striding in terms of internal loading on roller skis during sprint. The test subject will be a group of cross-country skiers and long-distance skiers. Results: Using the measured values of the internal loading and the measured time, we found differences during comparing the diagonal striding and double polling in specific values of SF, VO2 / kg, VE. the conclusion was that diagonal striding is faster than double poling to the uphill on roller skis. Keywords: XC-skiing, roller skis, Cortex MetaMax 3B, oxygen consumption, heart rate, lactate

Title: Climbing in children and youth: acute physiological responses to climbing and their implications for upper body strength. Objectives: The aim of this thesis was to determine the physiological responses of children and youth to climbing and its implications for upper body strength. Methods: One hundred and twelve children (aged 9,8 ± 1,4 years) participated across two studies. Study one (91 children) explored the effects of climbing on upper body strength, with the aid of a battery of climbing specific tests (bent arm hang on the trapeze, finger hang on the wooden bar and maximal hand grip with the manual dynamometer). These tests were designed for the measurement of the key muscle groups involved in climbing. Study two (21 children) assessed acute physiological response to climbing using the indirect calorimetry method. Energy expenditure was used as an indicator of the climbing skills and to express the total climbing work completed. Study two lasted for 16 weeks, during this time the children underwent three measurements. Instructors recorded the routes climbed and their difficulty during each session. The period of the study corresponded with the duration of the climbing course for children during the school year. The results of the acute physiological response of children during climbing...

Title: Comparison of the physiological response rate of the organism using different one-rope ascent techniques Objectives: The aim of this study was to compare the rate of the physiological response of the organism using 3 different one-rope ascent techniques during constant speed. Methods: It was an empirically based study of experimental character (quasi-experiment). The measured research group (n = 12) consisted of the military students (21 ± 1.1 years; 183 ± 4.9 cm; 80.6 ± 7.8 kg) of full-time study at the Faculty of Physical Education and Sport of Charles University (VO FTVS UK). The physiological response rate was measured with the Cortex Metamax 3b and Polar sporttester during one-rope ascending techniques ("using Prusik knot" = P", "using jümar = B", "using Garda knot = G") at a constant speed of 3 m·min-1 for 7 minutes. The rating of the perceived exertion was also recorded on the Borg RPE scale. The data were comparatively analyzed in the SPSS statistic program, furthermore the analysis of variance (ANOVA) was also employed. Results: The study demonstrated statistical differences (p ≤ 0.05) between techniques: while B is the easiest, the greatest differences were observed between technique B, P and G. The G technigue appeared to be the most difficult. The average HR was: 162 ± 9 bpm...

EFFECT OF DIFFERENT SIZES OF WHEELS OF IN-LINE SKATES ON ENERGY EXPENDITURE Objectives: The aim of this bachelor thesis is to determine the difference in energy expenditure when using different sizes of wheels of in-line skates. Methods: The research group consisted of 5 people in age 26,4 ± 2,2 years. This was an experiment. The calculation of energy expediture was based on the amount of oxygen consumption (VO2) and exhaled carbon dioxide (VCO2), i.e. indirect calorimetry. For the analysis of respiratory gases MetaMax 3B measuring device was used. Three sizes of wheels of the same hardness were tested. The rides were carried out on a 1,5 km section of a cycle path with the effect of the height difference of 2 mm/m. Probands completed one test ride (with a cadence of 70 rpm) on each size set of wheels. Test ride lasted for 5 minutes. For results were processed values of 3 minutes steady state VO2. Results: We found out a noticeable difference in energy output between 72 mm and 90 mm large wheels. The difference between driving on 72 mm and 80 mm large wheels and driving on 80 mm and 90 mm large wheels was, due to the standard error of measurement, insignificatnt. Conclusion: The energy expenditure reached a noticeable increase in driving with a larger diameter of wheels compared to driving on...

The aim of this thesis was to summarize current findings about exercise testing. The main goal was to evaluate protocols used in daily practice, their indications and benefits, comparability and parameters that can influence the results. The purpose of the practical part was to compare the maximal values of physiological parameters (heart rate, VO2, minute ventilation, RER, power output and rated perceived exertion) for the two most commonly used protocols for bicycle ergometry tests in the Czech Republic. We compared ramp (1/3W.kg-1 .min-1 ) and step (1W.kg-1 every 3 min) incremental protocols to exhaustion. Thirteen young, healthy and physically fit subjects (9 males and 4 women aged from 20 to 31 years) underwent two tests in separate occasions, during which they performed the protocols in random order. Paired t-test was used to statisticaly analyze the data. We found no statisticaly significant differences in these maximal values except for the exercise test time. We deduced some practical benefits of each protokol from obtained data.

The fluctuating course of tidal volume (VT), breathing frequency (DF) and minute ventilation (VE) during the cardio-pulmonary exercise test using a ramp incremental protocol occurs not only in patients, but relatively frequently also in healthy individuals. It can account for a number of irregularities in the course of the curves VO2, VCO2 and in particular of those of ventilatory equivalents for O2 and CO2 (EQO2, EQCO2) as well as curves of partial pressure of end-tidal oxygen and partial pressure of end-tidal carbon dioxide (PETO2, PETCO2), which are also used, inter alia, to establish ventilatory thresholds. The presence of exercise oscillatory ventilation (EOV) reflects the severity of heart failure and it is an independent predictor of the increased morbidity, cardiac and total mortality and sudden death caused by heart failure. However there is not a generally accepted universal definition of EOV available at present, as different criteria are used. We have not found a comparison which would indicate whether and how the "strength" of the prognostic criteria for EOV - established according to different methods - differs. Therefore it is very important to specify what method, or what criteria were used in the establishment of EOV.Key words: breathing pattern - EOV - exercise oscillatory ventilation - periodic breathing.

The article reviews the course of VO2 in the phase of recovery kinetics of oxygen uptake (VO2rec) cardiopulmonary exercise test. VO2rec kinetics cannot be completely defined using a single parameter. The article describes the three most frequently used methods: %VO2-REC2, T1/2 VO2rec and TC VO2, and provides detailed procedures of their analysis. The three aforementioned parameters are also at the heart of our classification of VO2rec. The method %VO2-REC2 is an essential one, in our view, since its prognostic significance is proven and it can be also easily applied only based on a data record of the cardiopulmonary exercise test. %VO2-REC2 should be seen, together with VO2peak, VE/VCO2 slope and exercise oscillatory ventilation (EOV) - as one of the four elementary prognostic markers establishing an advanced chronic heart failure.Key words: cardiopulmonary exercise test - chronic heart failure - prognosis - recovery kinetics of oxygen uptake.

Chronic Heart Failure (CHF) is accompanied by a whole range of symptoms, which significantly decrease the quality of life; these typically concern dyspnoea, fatigue and exercise intolerance. The objectification of the patients condition but must have an effective means of evaluation. One such resource is cardiopolmonary exercise (CPX) testing with it is functional parameters. CPX test with its functional parameters is one of such means. VO2peak is undoubtebly the parameter, which was used the most often in the past 25 years, however, it has many limitations. It is reason, why the evaluation of ventilation effectiveness using the VE/VCO2 slope is becoming more and more the centre of Czech and foreign interest of cardiologists abroad. Foreign studies have unambiguously proven that because of the absence of the fundamental limitations, such as in case of VO2peak, the VE/VCO2 slope is a stronger predictor of the amount of hospitalizations and of mortality in the population of patients suffering from CHF. This short overview intends to inform of the significant merit of this parameter. That is the reason why the VE/VCO2 should be classified as a standard criterion in indication of heart transplantations, but also in evaluation of seriousness and prognosis in population of patient suffering from HF.Key words: heart failure - mortality - prognosis - ventilation efficiency - VE/VCO2 slope.

Title: Energy expenditure of different size of mountain bike rims Objectives: The goal of this bachelor thesis is to gather and provide information on different energy expenditure (EE) of the organism when riding mountain bikes with different size of rims, while also considering different terrain. Methods: This thesis contains an experiment based on intra-individual measurements, which involved three participants of a similar age, weight, height and physical fitness. EE rate was determined based on the volume of oxygen consumption (VO2) and the amount of exhaled carbon dioxide (VCO2), i.e. by indirect calorimetry. For the measurement of respiratory gases device, we used Metamax 3B. The testing was performed with two wheel sizes, with diameters of 26 "and 29". The runs were carried out on natural circuit with different types of surface. Research participants completed two laps on both types of wheels. One ride lasted an average of 7:04 minutes. Results: We did not find a significant difference in energy expenditure (EE) when riding a mountain bike with the rims with a diameter of 26" and while riding on rims with a diameter of 29". The results of the experiment showed a slight difference in favour of the wheel diameter 29" however, regarding the standard error of measurement, this difference seems negligible...

Title: Comparison of physical load when moving in wet and dry clothes. Objectives: The aim of the this study was to compare the extent of the physical load when moving in wet and dry field uniforms pattern 95 at the speed of 5 km∙h-1 while changing the pitch angle of the treadmill. Methods: Quantitative research based on the interpersonal and intrapersonal comparative analysis Results: The measured data prove that the difference between the heart rates when moving in wet clothing and dry clothing is not significant. The average heart rate was 111 ± 21 beats per minute in the dry clothing and 108 ± 18 beats per minute in the wet clothing. During the average oxygen consumption a significant difference was observed only at the end of the measurement. At the end of the measurement in wet clothing, the measured values of oxygen consumption were on average 1,87 ± 0,69 mL∙min-1 ∙kg-1 higher than in dry clothing. The average value of oxygen consumption was 22 ± 8,20 mL∙min-1 ∙kg-1 in dry clothing and 22,96 ± 8,44 mL∙min-1 ∙kg-1 in wet clothing. Keywords: transfers, physical load, walking, heart rate, oxygen consumption, expiratory gases

Title: Effect of different types of bicycle tires on energy expenditure Objectives: The aim of this bachelor thesis is to determine the difference in energy expenditure when using different types of bicycle tires. Methods: The research group consisted of 5 males in age 26,4 ± 2,2 years. This was an experiment. The calculation of energy expediture was based on the amount of oxygen consumption (VO2) and exhaled carbon dioxide (VCO2), i.e. indirect calorimetry. For the analysis of respiratory gases MetaMax 3B measuring device was used. Three types of bicycle tires were tested. The test ride was carried out in laboratory on the cycling rollers. Probands completed one test ride (with a cadence of 80 rpm) on each type of tires. Test ride lasted on average 4:55 ± 1:50 min. For results were processed values of 4 minutes steady state VO2. Results: We found out the significant difference in energy expenditure between ride with road tires and ride with Fat Bike tires, and also between ride with trekking tires and ride with Fat Bike tires. The difference between ride with road and trekking tires was, due to the standard error of measurement, insignificant. Conclusion: The energy expenditure reached a significant increase while using tires with the largest contact patch compared to using tires with the smallest...

Aim of the search is to determine relations between results of the arm crank ergometry and overall fitness, to compare the results of cycle ergometry testing on one -legged ergomet er and two -legged ergometer. Adequate decision between use of one -legged ergometer or arm crank ergometer is the outcome. Powered by TCPDF (www.tcpdf.org)

The results of an experimental rearing of peled under different oxygen regimes are presented in this work. Four variants were tested in three repetitions (hypoxia 55-65 %, normoxia 85-95 %, permanent hyperoxia 145-155 % and alternate hyperoxia 145-155 % during the day and 85-95 % overnight). Also the oxygen consumption of fish with weight from 4.5 to 20 g at temperatures of 15, 19 and 23 °C was measured.

Title of master thesis Physiological response and physical activity during climbing wall in school age children Work objectives Determinaton of specific oxygen uptake during climbing in the climbing wall in children. Methods The study involved 10 boys and 9 girls. Their climbing ability was in range 4 to 5+ degree of UIAA (Union Internationale des Associations d'Alpinisme). Maximal oxygen consumption was measured on climbing wall. The climbers climbed two routes, the first vertical profile (90ř) and second overhanging profile (110˚). The participants climbed the wall with a self-selected speed. They climbed the route twice to steady state of physiological response during climbing and to simulate real average wall (15 m). To make conditions equal, everybody had 30 s to get down and start climbing again. Results The girls achieved average of specific oxygen consumption VO2peak 37,1 ± 4,8 ml·kg- 1 ·min-1 in the vertical profile and in the overhanging profile was 40,6 ± 11,5 ml·kg- 1 ·min-1 . The boys achieved slightly higher average of specific oxygen consumption in both profile 39,1 ± 4,8 ml·kg-1 ·min-1 in the vertical profile and 42,1 ± 2,6 ml·kg-1 ·min-1 in the overhanging profile. The girls had average peak heart rate 179 ± 10 beats·min-1 in the vertical profile and boys 170 ± 14 beats·min-1 ....

Title The energy performance at the growth rate in ski-mountaineering Objectives Measure group skialpinists of different ages and performance of the ski-mountaineering simulator with a gradient 21ř and speed 1,4 km/h to 5,4 km/h with increasing 0,4 km/h in every minute. Determine their energy expenditure in different speeds as the speed increases and with constant slope. Methods Seven ski-mountaineerers of different levels were testing in laboratory of ski-mountaineering simulator. We calculate of maximum oxygen consumption and anaerobic threshold. Results The probands attained average energy expenditure 8,18 kJ per minute at the end of the first minutes and they attained average energy expenditure 29,32 kJ per minute at the end of the 10th minutes. The average energy expenditure of probands increased by 2,35 kJ per minute an average with a standard deviation 0,69 kJ per minute. Keywords ski-mountaineering, heart rate, oxygen consumption, ski-mountaineer trainer.

Title: Metabolism of selected species of predatory aquatic insects. Target: The main objective of this study was to analyze metabolic rates of predatory aquatic insects, in my case dragonflies (order Odonata, suborder Anisoptera). Different instars of several species of dragonflies were observed. Another objective was to determine the dependence of the metabolic demands on the species and size of the individual, create tables and graphs summarizing the most important results and perform a basic statistical analysis. Methods: In my work I used the method of measuring the consumption of dissolved oxygen, using a polarographic determination of oxygen concentration with a membrane-covered oxygen sensor. Results: The rate of metabolism on the unit weight decreased with increasing weight and size, which corresponds to the assumptions of metabolic theory of ecology.

The aim of this thesis was to determine the effect of driving in a wheelchair and walking on crutches on the energy expenditure and cardiopulmonary system in people with lower limb amputation. For this measurement we used a device Metamax 3B made by Cortex, functioning by measuring the concentration of oxygen and carbon dioxide in the breathing air, and a sporttester made by Polar. On the basis of oxygen consumption is possible to determine an energy expenditure during the activity. Our study was attended by 11 probands (men), patient of long-term hospital in Motol Hospital. Nine probands were amputated from vascular causes, one from the traumatological causes and one from the other causes. Probands rode (or walked) for four minutes back and forth along the corridor thirty meters long. They were instructed to ride (walk) at a speed to fit their needstand to also keep the same pace for a defined period of time. Walking on crutches without prosthesis managed only three of the testing file. During the study, these values were measured: distance traveled, average speed, oxygen consumption (VO2, VO2/kg, VO2 peak), carbon dioxide output (VCO2), respiratory exchange ratio (RER), tidal volume (VT), breathing frequention (BF), minute ventilation (MV), heart rate (HR), energy expenditure and performance. In this work...

Aims of this thesis were to assesed the impact of feeding, temperature of water and fish size on ammonia excretion and oxygen consumption in Clarias gariepinus in recirculating system. Fish were divided in 8 weight categories from 21 to 2495 g. Experiments were performed under four temperatures: 22°C, 25°C, 28°C and 30°C. Fish were acclimatized, light conditions were: 14 hours of light period and 10 hours of dark period. Feeding was provided fed four times a day. Starved fish were measured. Measurements were held every two hours. Oxygen consumption was measured by multimeter and ammonia excretion using Nessler?s method. Average daily oxygen consumption in fed fish was dependent on temperature and average individual weight of fish, varied from 10.5 to 96.6 mg O2/kg/h and in hungry fish from 4.3 to 61.8 mg O2/kg/h. TAN excretion varied from 0 to 59.9 mg TAN/kg/h in fed fish and from 0 to 60.8 mg TAN/kg/h in hungry fish. Oxygen consumption increased with increasing temperature. Ammonia excretion was very unstable at all temperatures in all weight categories. Fish had higher ammonia excretion in light period of experiment.

The paper presents a protocol for the evaluation of energy metabolism in hepatocytes with the aid of high-resolution oxygraphy in permeabilized cells. Optimal conditions are suggested for the isolation of hepatocytes from the hepatic tissue of the rat and the proper technique for control of their structural integrity. Our work describes in detail the experimental schemes for oxygraphic evaluation of the individual enzyme complexes of the respiratory chain (Complex I, II, IV) and methods for the assessment of the mitochondrial function of phosphorylation using the respiratory control index and oligomycin inhibitory effect on ADP-activated respiration. On the example of oxidative damage of hepatocytes by tert-butylhydroperoxide, we demonstrate the utility of oxygraphy in the diagnostics of pathologic conditions caused by disturbances in energy metabolism of the cell.

THE PURPOSE OF THE STUDY was to verify whether rehabilitation programs improve both stress tolerance and peak oxygen consumption (pVO2) plus the consumption of oxygen at the level of anaerobe threshold (AT) in patients after myocardial infarction treated with beta-blockers. Our objective was to find out the differences in changes in the above indicators in elderly patients. THE SET of consisted of 2 groups of patients aged over 65 (56 +/- 6.1) aor = 65 let (69 +/- 4.7). The first group contained 96 and the second group 31 patients, respectively. Prior and after the rehabilitation program, stress echocardiography (SE) and symptom-limited spiroergometric test were performed in the patients.A statistically significant improvement in stress tolerance and in the oxygen consumptions indicators was recorded in the group of younger patients (65 years of age), both at the peak and at the anaerobe threshold levels (p0.001). Patients aged 65 or older recorded a statistically significant improvement in stress tolerance (p0.01) on the one hand, but only minor, statistically insignificant improvement in pVO2 and AT oxygen consumption on the other.A two-month rehabilitation program improves both stress tolerance and the peak oxygen consumption in patients after myocardial infarction treated with beta-blockers. The improvement is statistically insignificant in elderly patients. The above finding supports our opinion that elderly patients need long-term controlled training which should be performed at regular intervals and with the necessary intensity.

Aim of my work is influence of high level breeding of Euroasian perch on quality of water and operation of mechanicle drum filter and fluized bed biofilter. Euroasian perch is reared in recirculating aquaculture systeme. Fish were devide into six groups in dependence on their body weight. Groups of fish with avarage start weight (g) {$\pm$} SD; biomass (kg) 232,9 {$\pm$} 10,0; 15,14 (I), 196,4 {$\pm$} 8,1; 15,32 (II), 139,8 {$\pm$} 6,1; 15,10 (III), 102,4 {$\pm$} 5,5; 15,06 (IV), 59,4 {$\pm$} 5,5; 15,04 (V), 37,7 {$\pm$} 7,5; 15,00 (VI) were put into plastic tank (600l). Three measurement was with fed fish and three measurement with food-deprived fish. Fed day was organised like feeding period (light phase) 12 hours and 12 hours non-feeding period (dark phase). First part of my project was about impact mechanicle filtration to remove solids from water. Mechanical filter can keep the water quality on perfect level for fish farming. Second part was about evaluation of nitrifying biologickle filters. Biologicle filtration was did her function well, because levels of amonia, nitrite and nitrate were keep on optimum values. Third part of my project was about measurement of oxygen consumption and amonia excretion. Was observed significant diurnal changes of oxygen consumption and amonia excretion for fed fish. For food-deprived fish was observed significant diurnal changes of oxygen consuption. Changes of amonia excretion were not significant.

In recent years evidence is increasing on the usefulness of physical loads and controlled physical training in patients with chronic heart failure (CHSS). In the presented work the authors assessed changes of the functional capacity and muscular strength after training on a bicycle ergometer. The group comprised 38 patients with CHSS due to IHD or dilatation cardiomyopathy NYHA II-III, EF lower than 40%, with a peak oxygen consumption (pVO2) lower than 20 ml/kg/min. The group was subdivided in a random fashion to subjects participating in training (T) and the control group (K). The patients were subjected to clinical examination, examination by common laboratory methods, spiroergometry, dynamometry. By the puncture technique a specimen of the m. vastus lateralis was taken for histological and histochemical examination of the muscle. The patients trained on the bicycle ergometer three times per week for a period of eight weeks, one exercise session lasted 30 minutes and was at the level of the anaerobic threshold. After completion of the training period the examinations were repeated.Before the onset of training the groups did not differ in any indicators. After termination of training they increased in group T: pVO2 from 18.9 +/- 4.8 to 22.13 +/- 15.72 ml/kg/min. (p0.0004), the oxygen consumption at the level of the anaerobic threshold (VO2AT) from 13.4 +/- 3.4 to 15.96or = 3.75 ml/kg/min. (p0.0006), the respiratory quotient (RQ) from 0.93 +/- v0.09 to 0.97 +/- 0.006 (p0.05), the maximal tolerated load from 0.72 +/- 0.72 to 1.08 +/- 0.33 W/kg (p0.002), the maximal voluntary contraction of the femoral quadriceps muscle (MVC START) from 291.2 +/- 70.1 to 328.1 +/- 66.0 N (p0.01), the maximal voluntary contraction of this muscle after 20 mins. of repeated contractions (MVC END) from 157.6 +/- 109 to 290.1 +/- 64.9 N (p0.01), the decrease of the maximal contraction after 20 minutes of repeated contractions was from 52.8 +/- 32.1 to 12.4 +/- 5.0% (p0.01). After training there were statistically significant differences between groups in VO2AT (p0.01), in pVO2 (p0.03) and in the decrement of the maximal muscular contraction (p0.01). The authors found a trend towards normalization of the diameter of muscle fibres I and II and of their ratio. The ventilation equivalent for carbon dioxide VE/VCO2 during the maximal tolerated load correlated significantly with the systemic and pulmonary vascular resistance, with RQ, VO2AT, pVO2, with the maximal tolerated load and with the blood level of prostaglandin F.Controlled physical training in patients with CHSS was safe, led to a significant improvement of spiroergometric indicators, load tolerance and muscular strength. After training there was a trend towards normalization of pathological changes in skeletal muscle. Based on the authors' experience and findings of other authors it is advisable to recommend training as part of treatment of patients with CHSS.

Both the high body fatness and low aerobic fitness have been shown to be risk factors for cardiovascular disease and type 2 diabetes. It is still unclear, whether these factors are related to each other or if they are independent risk factors. The objective of this study was to assess the influence of body composition on aspects of aerobic fitness in obese women.Relative body fat was measured in a group of 31 obese women differing in age (25-54 years) by dual energy X-ray absorbtiometry (DEXA), in range of 40,8-58,8% of the body fat. Intracellular and extracellular fractions of the fat free mass were assessed by bioimpedance analysis. Maximal power output and maximal oxygen consumption were measured by stepwise load test on bicycle ergometer. Mean values of absolute and relative VO2max expressed per kg body mass and per kg fat-free mass were 2,09+/-0,38 l x min(-1), 22,82+/-3,79 ml x min(-1) kg(-1), and 44,05+/-7,03 ml x min(-1) x kg(-1), respectively. Absolute VO2max was positively related to body weight (R=0,4758; P0,01), BMI (R=0,5004; P0,01), fat-free mass (R=0,5138; P0,01) and body cell mass (R=0,4983; P0,01). No significant dependence of relative VO2max per kg body mass on the body fat was identified.Aerobic fitness, expressed by relative maximal oxygen consumption per kg of body weight, is not influenced by the percent of body fat in obese women. Body cell mass is markedly related to aerobic fitness, expressed by absolute maximal oxygen consumption in obese women.

The objective of the work was to evaluate the effect of eight-week combined training on the performance, aerobic capacity and basic haemodynamic parameters in patients with systolic dysfunction of the left ventricle and to assess its safety. The investigation comprised 26 patients, men mean age (x +/- SD) 61.8 +/- 11.1 years with coronarographically verified chronic ischaemic heart disease and with a left ventricular ejection fraction lower than 40% (EF 35 +/- 4%). Before the beginning and after completion of the rehabilitation programme (eight weeks) a spiroergometric examination was made, up to the symptom-limited maximum. Fitness elements were included after 2 weeks of aerobic training. The lesson lasted 60 mins. and included warming up (10 mins.), aerobic load on an ergometer with an intensity of the load at the level of the anaerobic threshold (20 mins.), the stage of fitness training on a combined training machine (20 mins) and the relaxation stage (10 mins). In the fitness stage the patients started to exercise at the 30% level, after two weeks at the 60% level 1-RM (one repetition maximum) The results showed after eight-week combined training a significant (p0.05) increase of the maximum achieved performance (from 104 +/- 27 to 132 +/- 32 W) in patients with systolic left ventricular dysfunction. There was a significant increase in the capacity of the transport system expressed by the value of the maximum oxygen uptake (from 1545 +/- 312 to 1740 +/- 359 ml.min-1) and MET (from 5.3 +/- 1.3 to 6.0 +/- 1.4). There was a significant decrease of the blood pressure at rest, systolic and diastolic, and of the baseline value of the heart rate at rest and of the "product rate, pressure"--RPP. Changes in the EF were not significant.

Regular physical activity can have a favourable impact on other risk factors of ischaemic heart disease (IHD) and associated diabetes (DM), such as obesity, hypertension, dyslipidaemia, insulin resistance and others. This important part of treatment of diabetes is frequently difficult to implement because of the lack of willingness ("adherence") of type 2 diabetics to practice regular exercise, and unequivocal data are lacking on the intensity of exercise which will influence effectively these risk factors and be at the same time safe, readily available and psychologically acceptable. The objective of the work was to find out whether walking, i.e. locomotor activity with a low to medium intensity can effectively influence parameters of aerobic capacity and blood lipids. The authors submit the results of two groups of type 2 diabetics. The experimental group B (n = 10, age 57 +/- 7 years, BMI 31 +/- 3, duration of DM 8 +/- 5 years) participated in a 12-week training programme of walking; at the beginning and at the end of this period indicators of aerobic capacity at the level of the anaerobic threshold (VO2ANP) were evaluated as well as at the level of the symptom limited maximum (VO2SL, TepO2SL), and the blood lipid levels. In the control group A (n = 6, age 58 +/- 7 years, BMI 32 +/- 4) indicators of aerobic capacity and blood lipids were assessed after a 12-week period of the usual habitual physical activity. In group B the 12-week walking training led to significant improvement of parameters of aerobic capacity at the level of the anaerobic threshold (ANP), oxygen pulse at the level of the symptom limits maximum (SL) and a significant reduction of total and LDL cholesterol. In the control group no significant changes occurred in aerobic capacity nor blood lipid values. The training programme where walking was selected as physical activity with a low to medium intensity can be considered suitable for everyday life of motivated patients with type 2 diabetics, preferably in the form of a domestic training programme. The prerequisite of success is its regular and frequent evaluation by health professionals.

The objective of the presented work is to evaluate to what extent the CO2 production and O2 utilization and energy metabolism at rest (REE) are influenced by an excessive nutrient intake and to what extent by the composition of enteral nutrition. REE, CO2 production and O2 utilization were investigated in 9 patients on complete enteral nutrition by indirect calorimetry in four modifications: I--nutrition with 40% kJ fat in a ration 1.2x the energy output at rest at the onset of the trial; II--40% kJ and high energy intake (2.4x energy output at rest; III--60% kJ fat in ration of 1.2x energy output at rest; IV--60% kJ fat and energy intake 2.4x energy output at rest. At 40% (I) and 60% (III) fat content in a caloriocally adequate diet the energy output at rest, the CO2 production and O2 utilization did not differ (mean +/- SD: 1438 +/- 264.1 kcal/24 h, 179 +/- 31.6 nl/min, 209 +/- 38.1 ml/min vs. 1431 +/- 342.7, 190 +/- 54.2, 207 +/- 46.5). Comparison of modifications II and IV revealed a significant (p0.05) increase of CO2 production on a diet rich in carbohydrates (218 +/- 52.0 vs. 202 +/- 42.3). The energy output at rest (1674 +/- 389.6 vs. 1661 +/- 378.7), nor O2 production (240 +/- 54.5 vs. 242 +/- 55.4) changed. Overfeeding with 40% fat (II) as compared with (I) led to a rise of the energy output at rest (p0.05), O2 utilization (p0.05) and CO2 production (p0.01). Overfeeding with lipids (IV) led as compared with III to a rise of the energy output at rest and O2 utilization; CO2 production did not change.The composition of enteral nutrition according to the described modification does not influences the energy output at rest, O2 utilization and CO2 production under conditions of an adequate energy intake. In case of an excessive nutrient intake nutrition with 60% fat does not lead to an increase of CO2 production.

The fully automatic non-radiometric cultivation system BACTEC MGIT 960 of Becton Dickinson is one of the systems using the so-called rapid metabolic method of mycobacteria cultivation. For detection of mycobacterial growth it uses the fluorescent technology. The objective of the present work is to compare the effectiveness of this system with classical cultivation on solid (egg) and liquid media. During the period from April 27, 1999 till April 28, 2000 in our department concurrently by both methods a total of 2144 samples were examined from which a total of 149 mycobacterial strains were isolated. It was revealed that the BACTEC MGIT 960 system is much more sensitive than classical cultivation (it detected 136 isolates as compared with 103 detected by the classical method): in isolation of Mycobacterium the difference in sensitivity is even more marked (from the total number of isolated strains of Mycobacterium tuberculosis the BACTEC MGIT 960 detected 105, classical cultivation only 76). The mean Time to Detection (TTD) of positive samples in the BACTEC MGIT 960 system was 13.2 days while for classical cultivation it was 33.6 days. It was thus confirmed that the BACTEC MGIT 960 achieves, as compared with classical cultivation, better results which applies to the sensitivity of the two methods as well as to the detection rate of positive samples.

Walking is at present the most frequently used means for purposes of rehabilitation as well as for maintenance or promotion of physical fitness. Recently we encounter with increasing frequency the use of walking as a diagnostic method for assessment of physical fitness under field conditions. The basis are motor tests of 1000 to 2000 m. The purpose of the submitted study was to prepare tables for the evaluation of the fitness level, using the 2000 m walking test and our standards of fitness.The basis of the majority of evaluations of the fitness level based on the motor test are relations between the intensity of motor activity and the maximal oxygen consumption. Using general relations between the average walking speed in the 2000 m test and the maximal oxygen consumption assessed on a treadmill in non-trained healthy men and women and base on our population standards (VO2max.kg-1) tables were elaborated. The fitness level is evaluated using the average walking speed in the 2000 m test and the appropriate maximal oxygen consumption. The tables have three grades and make it possible to evaluate men and women aged 14-65 years.The average speed in the 2000 m walking test makes it possible to evaluate by means of tables the physical fitness level in non-trained healthy subjects, provided the walking style is not of the racing type. The error of the estimate of physical fitness is about 15%.

Repeated exposure to cold leads to a decline in the intensity of physiological defence reactions. In the complex of adaptational responses metabolic, thermogenic and insulation reactions predominate. The objective of the present investigation was to provide evidence of the predominating type of response of sportsmen exposed repeatedly to cold in an aqueous environment and to elucidate metabolic processes which serve to meet the energy demands of a certain type of adaptation to cold.The examination was made in a group of volunteers (men, mean age 21.0 +/- 1.8 years, height 1.80 +/- 0.04 m, body weight 77.0 +/- 3.1 kg, body fat 13.2 +/- 1.7%, anaerobic capacity expressed as maximum oxygen consumption 4.5 +/- 0.43 l.min-1). The cold acclimation programme involved immersion of the body into water 14 +/- 1 degree C 3 times a week for 1 month. During cold exposure the median body temperature before the acclimation programme dropped by 3.9 degrees C, after the acclimation programme by 4.1 degrees C. The total metabolic turnover after the programme declined by a 0.23 multiple of the metabolism at rest, at the end of the programme merely by a multiple of 0.07. During immersion the oxygen consumption doubled regardless of the stage of adaptation and an insignificant rise of non-esterified fatty acids occurred before the onset of the programme as well as after its termination. After the first exposure to cold a significant drop of the blood sugar was observed, while after the acclimation programme it increased insignificantly. The serum level of the thyroid stimulating hormone and triiodothyronine were affected only insignificantly by exposure to cold, the thyroxine level only at the borderline of significance before the onset of the acclimation programme.From values of the blood sugar level, respiratory quotient RQ, the non-esterified fatty acid level and body temperature at the periphery we may conclude that there is a restriction of the carbohydrate component as regards meeting energy demands of cold exposure before adaptation and a mixed type of meeting energy demands after termination of the cold acclimation programme.

The authors compared two types of spiroergometric tests in 14 patients with chronic heart failure (NYHA II-III, ejection fraction40%). Test A: 0.25 W/kg 3 minutes, 1 minute break, increase by 0.25 W/kg á 3 minutes. Test B: 25 W 2 minutes without break, increase by 10 W á 2 minutes. The two tests did not differ as to the achieved heart rate, blood pressure reading, oxygen consumption and biochemical parameters at the end of the load, even at the level of the anaerobic threshold. There was a significant statistical difference in the duration of the load: test A lasted 16.4 minutes, test B 9.7 minutes (p0.001). The length of the test B correlated with the peak oxygen consumption per 1 kg body weight (p0.001, r 0.9866). The authors recommend for common practice test B with a defined period of the load as sufficient.

The authors investigated and compared the response of 26 men with insulin dependent diabetes mellitus to a physical load in the laboratory (bicycle spiroergometry with a work load graded up to a maximum) and recommended 20 min. walking or running under field conditions (constant speed beneath the "anaerobic threshold"). The mean maximal oxygen uptake per minute in the laboratory (2.81 +/- 0.47 l) was insignificantly lower than reference values and combined with the maximal minute heart rate (mean 175 +/- 17) very variable. During the load under field conditions the oxygen uptake practically did not change (1.64 +/- 0.59 l/min.) but the heart rate increased due to the declining stroke volume. The blood sugar level in the laboratory and during field exercise declined significantly. In the laboratory the base deficit and lactacidaemia increased markedly; under field conditions their concentration increased slightly and did not pass the anaerobic threshold level. The pyruvate, acetoacetate and beta-OH-butyrate concentrations did not change much and were consistent with the response of well controlled diabetic patients.

The energy requirement of walking, expressed indirectly by the oxygen consumption per kg body weight was assessed during different speeds of walking on a treadmill within the range of 3-12 km.h-1 in 87 untrained healthy men aged 17.5 to 60 years. The dependence of VO2.kg-1 on the speed of walking is non-linear in the mentioned range. Most suitable is the two-component linear model which in the range of 3-7 km.h-1 has the shape of VO2.kg-1 (ml.kg-1.min-1 = 3.207.v/km.h-1 - 1.777, r = 0.932 with a mean predicting error of 1.5.ml.kg-1.min-1, in the range of load of 7-12 km.h-1 VO2 = 7.120.v - 29.168, r = 0.941 with a error predicting of 3.73. The polynomic model of relations in the entire range of 3-12 km.h-1 is VO2 = 4.503 - 0.108.v + 0.379.v2, r = 0.922 with a predicting error of 4.43 ml.kg-1.min-1, and finally the exponential model has the shape VO2 = 4.360 exp (0.223.v), r = 0.861 with a mean predicting error of 6.84 ml.kg-1.min-1 in the entire range of load intensities. The justification to express the relationship between the oxygen consumption and the rate of walking by a two-component linear model as well as by non-linear models is confirmed also by the high correlation coefficient (p0.001 in all instances). The error of assessment of VO2.kg-1 from the speed of walking is 10% or less in all models. The mentioned models, in particular the linear one, can be used for evaluation of physical activities involving walking outdoors.

The authors investigated the development and interrelations of respiratory and biochemical parameters of normal neonates during the adaptation period from 15 minutes to the 7th day after delivery. They found that the correlations of oxygen consumption, carbon dioxide output, respiratory volume and minute ventilation are positive and independent on the time of assessment. These respiration parameters are independent on the glucose, lactate and pyruvate levels, on the respiratory quotient and pH and pCO2. The authors characterize the similarity of the trend of individual parameters and the variability of normal values.

In the past some authors revealed that data assembled during examinations of the central haemodynamics, echocardiography, subjective complaints, physical findings and load tolerance in patients with chronic heart failure are not mutually consistent. The authors examined therefore comprehensively 40 patients with different grades of cardiac failure. They revealed very loose relations between the grade of functional classification, echocardiographic and invasive haemodynamic parameters at rest on the one hand and spiroergometric indicators on the other hand. Closer relations with spiroergometric findings were obtained with values of invasive haemodynamic parameters after a load, in particular values of the cardiac index, and systemic vascular resistance. The authors conclude that for clinical manifestations of chronic cardiac failure the peripheral circulation and tissue metabolism is at least equally important as changes of central haemodynamics. Moreover, the importance of the right ventricular function and diastolic cardiac function is not sufficiently appreciated.

The reliability and validity of assessment of the maximal oxygen consumption as a criterion of aerobic fitness by the 2000 m walking test was established in 38 students of the military secondary school (mean age 17.8 +/- 0.4 years). The reliability of the 2000 m walking test is very high, during repeated estimations in the course of one week we find a correlation coefficient of r = 0.82 (p0.001). There is also a close correlation of 2000 m walking time and 2000 m running time (r = 0.67, p0.001). A group of 18 students selected at random from the above group was examined in the laboratory on a treadmill up to vita maxima. A close correlation was found between the results during walking and the maximal oxygen consumption (VO2max.kg-1) (r = -0.59 and -0.60) and also the 2000 m running time and VO2max.kg-1 (r = 0.59), p0.01 in all instances. The standard error of the mean of the maximal oxygen consumption estimate from the 2000 m walk is 3.44 ml.kg-1 x min-1 (5.7%) and from the running time of the same distance 3.49 ml.kg-1 x min-1 (5.8%). These results justify the conclusion that the 2000 m rapid walking test (normal style) is a simple alternative way for estimating the maximal oxygen consumption and thus also for evaluating the level of aerobic fitness with an accuracy which matches that, when running is used for the test.

The submitted paper is devoted to some aspects of metabolic and immunity reactions of the organism to extracorporeal circulation. The authors focused attention in particular on a correlation of the whole-body oxygen consumption and the blood sugar level. Surprising findings comprise the rise of the whole-body oxygen consumption which occurs before the tissue temperature rises. As far as toxic oxygen radicals are concerned, their release is individual. It is, however, important that during the perfusion proper the activation of the immune system is suppressed. The course of the extracorporeal circulation was satisfactory from the clinical aspect; despite that it cannot be considered physiological, as also apparent from some biochemical findings.

The bicycle ergometer is the most frequently used equipment for loading tests for the purpose of functional diagnosis and for the purpose of locomotor rehabilitation. The energy required to meet this locomotor activity is within a wide range of load intensities which depends linearily on the intensity of pedalling. The steepness of this linear relationship which characterizes the degree of adaptation of the organism to this type of locomotor activity is the smaller the more the investigated subjects are adapted to the load. In general it depends on the degree of training, age, sex, strength, pedalling frequency, on the load protocol and on the biomechanical conditions during pedalling (e.g. the height of the saddle). Based on our measurements in a group of untrained women of different age and in trained women of different age it can be demonstrated that all individual relations between energy characterized by means of VO2 and the intensity of the load - P is within the zone where the upper borderline is formed by the equation VO2(ml) = 11.00.P (W) + 403 and the lower borderline by VO2(ml) = 9.90.P (W) + 252. The basic relationship, independent on the degree of training, age and strength, valid in the zone of pedalling frequencies of 60-80 rot. min-1 and in the zone of load intensities of 75-325 W has the shape of VO2(ml) = 10.60.P(W) + 269. The maximum error of assessment of VO2 by means of the load intensity is 9% or less.

The authors investigated in a group of 83 patients with chronic obstructive pulmonary disease the prognostic impact of some indicators of pulmonary function, haemodynamics and oxygen transport. They demonstrated that the prognosis is less favourable in patients with a lower one-second vital capacity with a higher median pressure in the pulmonary artery, with a lower oxygen tension in the arterial blood and higher haemoglobin concentration. They did not reveal significant differences between those who died and those who survived for more than five years, after the examination, as regards oxygen supply of tissues, oxygen consumption, coefficient of oxygen extraction and values of oxygen tension in mixed venous blood. Oxygen inhalation led to an increased oxygen supply of tissues and increased oxygen tension in mixed venous blood to normal values in all examined groups. This observation may be one of the explanations of the more favourable prognosis of patients with respiratory insufficiency, associated with chronic pulmonary obstructive disease, who have permanent oxygen therapy during domiciliary care.