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Triacylglycerols are among the most abudant classes of lipid. Their chemical, physical and biological characteristics depend on degree of unsaturation and positions of double bonds in acyls. This thesis was focused on localization of double bonds using tandem mass spectrometry with atmospheric pressure chemical ionization. We studied 14 standards and 44 standard mixtures with different number of double bonds and lenght of hydrocarbob chain in acyls. Standards were obtained from commercial sources and prepared of randomizations reaction in microscale. Localization of double bonds was deduced from fragments of molecular aduct with C3H5N+· ([M+55]+·) created in presence of acetonitrile in the mobile phase. This metod was applied for HPLC/MS analysis of natural mixtures of triacylglycerols. Key words: triacylglycerols, randomization, localization of double bonds, atmospheric pressure chemical ionization, mass spectrometry, high performance liquid chromatography with mass detection

The topic will be divided into two parts {--} theoretical and practical ones {--} and a conclusion with an evaluation of the project. The theoretical part will include the basic data concerning developmental psychology together with a deeper insight into a period of adulthood and middle- and post-middle ages. It will be an outlook of a healthy population that deals with common situations of everyday life, with a period of their and their children´s maturity, with a choice of their first partner for life, with a departure of the partner and finally with their children´s leaving and arranging their own lives. My work will include some systematic procedures and casuistics among the selected clients. I will focuse on solving the common situations of everyday life in a Simulation studio together with an effort to approach a so called {\clqq}Healthy Rožnov Drawing``. The practical part will analyse the client´s work. In a conclusion I want to summarise my long-term activities with the above mentioned clients.