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The Body Condition Score (BCS) is an important tool for the evaluation of the horse's nutritional status during the welfare assessment [1]. However, its main limitation is its subjective nature [2]. This problem could be overcome with the use of objective techniques. Microwave human body imaging technique is applied to analyse reflections from skin, fat and muscle tissue borders. Therefore, the aims of the present study are: (i) to obtain preliminary results on the feasibility of the use of microwave frequencies on different ex vivo horse tissue samples; (ii) to characterize the complex permittivity of the horse tissues