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The translatability of research is highly dependent on models that recapitulate human tissues and organs. Here, we describe a procedure for the generation of human epidermis organotypic cultures (HEOCs) from primary keratinocytes isolated from foreskin and adult skin as well as from an immortalized keratinocyte cell line (KerTr). We tested several media conditions to develop a defined HEOC growing and expansion media. We characterized the HEOCs and show that in optimal culture conditions they express the proliferation marker Ki67, the basement membrane protein collagen 17 (col17) and the epidermal differentiation markers keratin 15 (K15), keratin 14 (K14), keratin 5 (K5), keratin 10 (K10), keratin 1 (K1), transglutaminase 1 (TGM1), transglutaminase 3 (TGM3) and filaggrin (FLG). Thus, they recapitulate the human epidermis and are stratified from the basal layer to the stratum corneum. These HEOC can be generated reproducibly on a large scale, making it an invaluable model for screening therapeutic compounds and also for the study of pathologies affecting the epidermis. [ABSTRACT FROM AUTHOR]

The objective of this article is to determine how Indian law can resolve the conflict of dual loyalties faced by nominee directors. We first study nominee directors' duties towards their nominator in commercial practice, and directors' duties under the stakeholder-oriented provision of section 166(2) in Indian Companies Act 2013. We then take stock of the judicial approaches towards nominee directors in the United Kingdom and in India. The Companies Act 2013 is presently inadequate to resolve the identified conflict. We suggest codifying a pragmatic approach by allowing nominee directors to pursue their nominator's interests, subject to the company's interests. To determine the company's interests, we refer to the Entity Maximisation and Sustainability Approach proposed by Andrew Keay that allows for a plurality of interests to be considered by a director, while providing a clear objective. In concluding, we call for further deliberation on the enforcement of the nominee directors' duties. [ABSTRACT FROM AUTHOR]

In the article, the authors present a case of a 16-year-old Hindu rural woman of middle socioeconomic status who was rushed to a clinic due to complaint of mutism to discuss the condition called anorexia nervosa with catatonia. Also cited are the symptoms of anorexia nervosa like very low body weight, disturbed body perception and intense fear of weight gain, and her prescription of lorazepam with significant improvement on her condition.