Search

In this study, a pH-responsive polycaprolactone (PCL)–copper peroxide (CuO2) composite antibacterial coating was developed by suspension flame spraying. The successful synthesis of CuO2 nanoparticles and fabrication of the PCL-CuO2 composite coatings were confirmed by microstructural and chemical analysis. The composite coatings were structurally homogeneous, with the chemical properties of PCL well maintained. The acidic environment was found to effectively accelerate the dissociation of CuO2, allowing the simultaneous release of Cu2+ and H2O2. Antimicrobial tests clearly revealed the enhanced antibacterial properties of the PCL-CuO2 composite coating against both Escherichia coli and Staphylococcus aureus under acidic conditions, with a bactericidal effect of over 99.99%. This study presents a promising approach for constructing pH-responsive antimicrobial coatings for biomedical applications. [ABSTRACT FROM AUTHOR]

To avoid the unexpected aggregation and reduce the cytotoxicity of nanomaterials as optical probes in cell imaging applications, we propose a programmed DNA-cube as a carrier for silver nanoparticles (Ag NPs) to construct a specific hydrogen sulfide (H2S) responsive platform (Ag NP@DNA-cube) for diagnosing colorectal cancer (CRC) in this study. The DNA-cube maintains good dispersion of Ag NPs while providing excellent biocompatibility. Based on the characteristic overexpression of endogenous H2S in CRC cells, the Ag NPs are etched by H2S within target cells into silver sulfide quantum dots, thereby selectively illuminating the target cells. The Ag NP@DNA-cube exhibits a specific fluorescence response to CRC cells and achieves satisfactory imaging. [ABSTRACT FROM AUTHOR]

Intensive economic and human activities present challenges to the carbon storage capacity of terrestrial ecosystems, particularly in arid regions that are sensitive to climate change and ecologically fragile. Therefore, accurately estimating and simulating future changes in carbon stocks on the northern slope economic belt of Tianshan Mountains (NSEBTM) holds great significance for maintaining ecosystem stability, achieving high-quality development of the economic belt, and realizing the goal of "carbon neutrality" by 2050. This study examines the spatiotemporal evolution characteristics of the NSEBTM carbon stocks in arid regions from 1990 to 2050, utilizing a combination of multi-source data and integrating the Patch-generating Land use Simulation (PLUS) and Integrated Valuation of Ecosystem Services and Trade-offs (InVEST) models. Additionally, an attribution analysis of carbon stock changes is conducted by leveraging land use data. The findings demonstrate that (1) the NSEBTM predominantly consists of underutilized land, accounting for more than 60% of the total land area in the NSEBTM. Unused land, grassland, and water bodies exhibit a declining trend over time, while other forms of land use demonstrate an increasing trend. (2) Grassland serves as the primary reservoir for carbon storage in the NSEBTM, with grassland degradation being the leading cause of carbon loss amounting to 102.35 t over the past three decades. (3) Under the ecological conservation scenario for 2050 compared to the natural development scenario, there was a net increase in carbon storage by 12.34 t; however, under the economic development scenario compared to the natural development scenario, there was a decrease in carbon storage by 25.88 t. By quantitatively evaluating the land use change in the NSEBTM and its impact on carbon storage in the past and projected for the next 30 years, this paper provides scientific references and precise data support for the territorial and spatial decision making of the NSEBTM, thereby facilitating the achievement of "carbon neutrality" goals. [ABSTRACT FROM AUTHOR]

Based on data from the Gurbantunggut Desert, the largest fixed/semi-fixed desert in China, and ERA5-Land reanalysis data, the long-term variations and spatial surface heat source (SHS) differences in the Gurbantunggut Desert are discussed herein. The results show the following: (1) The hourly SHS at the Kelameili station during the 2013–2021 period was a weak heat source at night; contrastingly, it was a strong heat source during the day. The duration of the hourly SHS increased gradually from January to July, but it decreased gradually from July to December. The daily SHS showed obvious seasonal variation, reaching the maximum in summer and the minimum in winter. The ERA5-Land reanalysis can reproduce all the variation characteristics of the SHS well. (2) The climatology (i.e., multi-year mean) of the monthly SHS intensity was lower than 50 W/m2 during the January–March and September–December periods in the Gurbantunggut Desert, indicating a weak heat source. On the other hand, the climatology recorded in April–August was higher than 50 W/m2, with a strong heat source. From the perspective of spatial distribution, the eastern and western regions of the Gurbantunggut Desert show strong heat sources, while the central region shows weak heat sources. The spatial distribution of the first and second modes of the empirical orthogonal function (EOF) decomposition reflected the consistent spatial variability and a north–south (or east–west) polarity variation of the monthly SHS in the Gurbantunggut Desert, respectively. (3) The yearly SHS showed negative anomalies during the 1950–1954, 1964–1982 and 2004–2015 periods, and positive anomalies during the 1955–1963, 1983–2003 and 2016–2021 periods in the Gurbantunggut Desert. Additionally, the time series of the SHS anomalies was positively correlated with the Interdecadal Pacific Oscillation (IPO) index. During the negative IPO phase, the yearly SHS showed a negative anomaly in the Gurbantunggut Desert, while the yearly SHS showed a positive anomaly during the positive IPO phase in most regions of the Gurbantunggut Desert. [ABSTRACT FROM AUTHOR]

Urban heat islands have garnered significant attention due to their potential impact on human life. Previous studies on urban heat islands have focused on characterizing temporal and spatial variations over longer periods of time. In this study, we investigated the urban heat island (UHI) in Birmingham from September 2013 to August 2014 using higher temporal resolution SEVIRI satellite surface temperature data along with data from the Birmingham Urban Climate Laboratory (BUCL) meteorological station and the UK Meteorological Office meteorological station. Our aim was to characterize the diurnal variations in the surface urban heat island intensity (SUHII) and canopy urban heat island intensity (CUHII) and to explore their relationship under the influence of three factors (day/nighttime, season, and wind speed) using regression analysis. Our findings reveal that SUHII and CUHII exhibit relatively stable patterns at night but vary significantly during the day with opposite diurnal trends. In addition, SUHII and CUHII were more variable in spring and summer but less variable in winter. During the nighttime, SUHII represents CUHII with high confidence, especially during spring and summer, but less so during the cold season. In addition, SUHII represents CUHII with greater confidence under low-wind conditions. This study deepens our understanding of the diurnal dynamics of urban heat islands and the influence of atmospheric conditions on the relationship between surface and canopy heat islands in urban areas. The results of this study can be used for heat island studies in cities that lack high-precision observation networks and to guide sustainable urban development. [ABSTRACT FROM AUTHOR]

Grassland ecosystems are an important component of global terrestrial ecosystems and play a crucial role in the global carbon cycle. Therefore, it is important to study the carbon dioxide (CO2) process in the Middle Tien Shan grassland ecosystem, which can be regarded as a typical representative of the mountain grasslands in Xinjiang. Eddy covariance (EC) and the global carbon fluxes dataset (GCFD) were utilized to continuously monitor the Middle Tien Shan grassland ecosystem in Xinjiang throughout the 2018 growing season. The findings revealed notable daily and monthly fluctuations in net ecosystem exchange (NEE), gross primary productivity (GPP), and ecosystem respiration (Reco). On a daily basis, there was net absorption of CO2 during the day and net emission during the night. The grassland acted as a carbon sink from 6:00 to 18:00 and as a carbon source for the remaining hours of the day. On a monthly scale, June and July served as carbon sinks, whereas the other months acted as carbon sources. The accumulated NEE, GPP, and Reco during the growing season were −329.49 g C m−2, 779.04 g C m−2, and 449.55 g C m−2, respectively. On the half-hourly and daily scales, soil temperature (Ts) was the main contributor to CO2 fluxes and had the greatest influence on the variations in CO2 fluxes. Additionally, air temperature (Ta) showed a strong correlation with CO2 fluxes. The grassland ecosystems exhibited the strongest CO2 uptake, reaching its peak at soil temperatures of 25 °C. Moreover, as the air temperatures rose above 15 °C, there was a gradual decrease in NEE, while CO2 uptake increased. The applicability of GCFD data is good in the grassland ecosystem of the Middle Tien Shan Mountains, with correlations of 0.59, 0.81, and 0.73 for NEE, GPP, and Reco, respectively, compared to field observations. In terms of remote sensing spatial distribution, the Middle Tien Shan grassland ecosystem exhibits a carbon sink phenomenon. [ABSTRACT FROM AUTHOR]

Land–atmosphere interactions are influenced by the earth's complex underlying subsurface, which in turn indirectly affects atmospheric motion and climate change. Human activities are increasingly exerting an influence on desert ecosystems, and artificial green land with clear functional orientation has been established in many desert areas. Consequently, the previously dominant, shifting, sand-covered, underlying surface in these desert regions is gradually transforming. This transformation has significant implications for the characteristics of land–atmosphere interactions, causing them to deviate from their original state. At present, existing studies still have not presented a systematic understanding of this change and have ignored the impact of human activities on land–atmosphere interactions in artificial green land. To address these research gaps, this study specifically targets artificial green land in the Tazhong region of Taklamakan Desert. We carried out observation experiments on land–atmosphere interactions in three different functional units from outside to inside: natural shifting sands, the shelter forest, and the living area. We also analyzed the differences and attribution of land–atmosphere interactions characteristics of different functional units. Compared with the natural shifting sands, the daily average maximum values of wind speed in the shelter forest decreased by 78%, and the daily average maximum air temperature and soil (0 cm) temperature decreased by 2.6 °C and 7 °C, respectively. Additionally, the soil moisture level was significantly increased throughout the green land due to the shelter forest. The surface albedo experienced a decrease, with an annual average of 0.21. Furthermore, the aerodynamic roughness and bulk transport coefficient increased by two orders of magnitude. The daily average maximum values of sensible heat flux and soil heat flux (G05) decreased by 18.7% and 75%, respectively, and the daily average maximum value of latent heat flux increased by 70.3%. This effectively improved the microclimate environment of the green land. The living area was greatly reduced by the shelter forest coverage and influenced by the buildings. Consequently, the environmental improvement was not as large as it was inside the shelter forest. However, it still provided a good shelter for production and living in the desert area. Throughout the year, a total of 4.60 × 105 t water was consumed through evapotranspiration in the artificial green land. The findings of this study have the potential to enhance our comprehension of land–atmosphere interactions in desert regions, thereby offering valuable insights for the establishment and effective management of artificial desert green lands. [ABSTRACT FROM AUTHOR]

High quality processing key to improving product quality and enterprise benefits. In this work, an adaptive network–based fuzzy inference system (ANFIS) was combined with milling experiments to understand the effects of tool geometry and milling parameters on the surface quality of wood fibre–reinforced magnesium oxide composite (WRMC). Specifically, changes in surface roughness (Ra) and damage of WRMC at different milling conditions were assessed using ANFIS and micro-analysis methods. Development of ANFIS models were confirmed to be reliable for predicting surface roughness. Changes in surface roughness at different milling conditions were determined, and the lowest surface roughness was obtained at the highest rake angle, highest cutting speed, and smallest milling depth. Furthermore, pitting-type damage irregularly distributed on the machined surface is attributed to the pulling out and debonding of wood fibres. Overall, high cutting speed, shallow cutting depth, and high rake angle is recommended for fine machining of WRMC where a smooth surface is desired. This study showcases how neuro-fuzzy models can be combined with conventional micro-analysis to optimize milling parameters for WRMC to minimize surface damage, and paves the way for future studies to optimize cutting tool life and energy consumption. [ABSTRACT FROM AUTHOR]

Beech wood is a material commonly used for furniture, and cutting performance is the key to improving product quality and enterprise benefits. In this work, beech milling experiments using diamond cutters were carried out, and the changes in cutting power, temperature, and surface roughness were examined using the factor analysis method. The main results of this work are listed as follows: Firstly, a higher cutting speed and depth led to higher cutting power, temperature, and surface roughness. Meanwhile, cutting power and surface roughness were negatively related to the rake angle; however, cutting temperature first increased and then decreased with the increase in rake angle. Furthermore, cutting depth had greatest impact on the cutting power and surface roughness, followed by rake angle and cutting speed. Cutting speed had the greatest contribution to the cutting temperature, followed by cutting depth and rake angle. Only the cutting depth had a significant contribution to both cutting power, temperature, and surface roughness. Finally, optimal cutting parameters were determined to be a rake angle of 15°, cutting speed of 54 m/s, and depth of 0.5 mm. These values best meet the multiple objectives of lower cutting power, temperature, and surface roughness, which relate to superior product quality and enterprise benefits. [ABSTRACT FROM AUTHOR]

The exchange of energy between the land surface and atmosphere is dependent upon crucial parameters, including surface roughness, emissivity, bulk transfer coefficients for momentum (CD) and heat (CH). These parameters are calculated through site observation data and remote sensing data. The following conclusions are drawn: (1) the aerodynamic roughness of the Gurbantunggut Desert measures 1.1 × 10−2 m, which is influenced by the varying conditions of the underlying surface. The roughness decreases as wind speed increases and is seen to be directly proportional to the growth of vegetation. From April to June, the aerodynamic roughness increases with increasing vegetation cover, but begins to gradually decrease after July. Spatially, the middle regions show higher roughness values than the eastern and western areas. In the central part of the desert, the roughness is between 2.37 × 10−2 m and 2.46 × 10−2 m from April to November. The northwest and northeast regions measure 1.41 × 10−2 m–2.04 × 10−2 m and 1.53 × 10−2 m–2.39 × 10−2 m, respectively. (2) The surface emissivity is 0.93, and it varies depending on the snow and vegetation present in the underlying area. (3) CD and CH exhibit an inverse relationship with wind speed. When wind speed falls below 6 m/s, the CD declines rapidly as wind speed increases. In contrast, once wind speed surpasses 6 m/s, the propensity for the CD to decrease with increasing wind speed slows down and approaches stability. [ABSTRACT FROM AUTHOR]

Cutting force and temperature are critical indicators for improving cutting performance and productivity. This study used an up-milling experiment to ascertain the effect of tool tooth number, cutting speed, and depth on the machinability of bamboo–plastic composite. We focused on the changes in the resultant force and cutting temperature under different milling conditions. A response surface methodology was used to build prediction models for the resultant force and temperature. A verification test was conducted to prove the model's reliability. The empirical findings suggested that the number of tool teeth had the most significant impacts on both the resultant force and the cutting temperature, followed by the depth of cut and the cutting speed. Moreover, the resultant force and cutting temperature showed increasing trends with decreasing numbers of tool teeth and increasing cut depths. However, cutting speed had a negative relationship with the resultant force and a positive relationship with temperature. We also determined the optimal milling conditions with the lowest force and temperature: four tool teeth, 300 m/min cutting speed, and 0.5 mm depth. This parameter combination can be used in the industrial manufacture of bamboo–plastic composite to improve tool life and manufacturing productivity. [ABSTRACT FROM AUTHOR]

Wind profile light detection and ranging (LiDAR) is an important tool for observing features within the atmospheric boundary layer. Observations of the wind field and boundary layer height from coherent Doppler wind LiDARs (CDWLs) under sandy and dusty weather conditions were evaluated using observations from two CDWLs and one GTS radio sounding located at the northern edge of the Tibetan plateau from 1 May to 30 August 2021. The results showed that CDWL has good applicability in reproducing wind fields in dust, precipitation, and in clear-sky conditions, and that it is superior to the v wind field for real measurements of the u wind fields. In terms of the planetary boundary layer height (PBLH), the validity of the inversion of PBLH in dusty weather was higher than that under clear-sky conditions. It was found that the PBLH retrieved by the CDWL at 20:00 (BJT) was better than that at 08:00 (BJT). The diurnal variation amplitude of the PBLH before the occurrence of a sandstorm was larger than the diurnal variation amplitude of the PBLH occurring during a sandstorm. [ABSTRACT FROM AUTHOR]

Background: Tobacco use is a global public health concern due to its association with a myriad of health hazards and costs. Previous studies have found that the disinhibiting influence of alcohol consumption may lead to an increase in tobacco use. In this study, we investigated why alcohol consumption is associated with tobacco use among older adults in Western China, and we attempted to explain the mechanisms that underlie the link between alcohol consumption and tobacco use. Methods: In this cross-sectional study, we employed a representative sample of 812 urban individuals aged 60 and older in 2017 in Western China. Results: We found that alcohol consumption (OR = 2.816, 95%CI: 1.770–4.479) was positively associated with tobacco use. Additionally, other factors, including gender (OR = 0.023, 95%CI: 0.009–0.060), age (OR = 0.758, 95%CI: 0.634–0.906), health literacy (OR = 0.977, 95%CI: 0.959–0.995) and functional impairment (OR = 0.871, 95%CI: 0.767–0.988) were also strongly related to tobacco use. Conclusions: Consistent with the results of existing studies, our findings demonstrated the disinhibiting influence of alcohol consumption on tobacco use, which confirmed the theoretical mechanisms that underlie the alcohol–tobacco link and implied the co-occurrence and co-use of alcohol and tobacco, which are used as "social lubricants" in the Chinese context. The importance of other factors related to tobacco use were also discussed. As the tobacco epidemic is combatted, joint interventions tailored to concurrent control over alcohol and tobacco need to be performed, and corresponding male-specific and age-specific health education and the reinforcement of health literacy must be considered as important priorities. [ABSTRACT FROM AUTHOR]

The emission, transport, and deposition of dust are frequently the focus of dust aerosol studies. However, owing to limited observation data, few studies have examined the relationships between the key parameters of dust transport, especially in typical dust source areas. Therefore, investigating the relationship between near-surface horizontal dust fluxes and dust depositions in typical desert source areas can further help us to understand the movement and transport patterns of dust aerosols. Based on observational experiments on two key transport parameters, this study focused on the quantitative relationship between the horizontal dust flux (Q) and dust deposition (D). A 13-month long dust sample collection experiment was conducted from August 2017 to August 2018 at Xiaotang Station (hereafter XT,40°48′ N, 84°18′ E) and Tazhong Station (hereafter TZ, 39°00′ N, 83°40′ E). The results show that the observed D and Q peaks coincided with periods of high dust storm incidence, with the greatest accumulation observed in spring. Moreover, both Q and D decreased with increasing height in XT, and this phenomenon remained on a monthly scale. In contrast, no clear decreasing pattern in Q and D with increasing height was observed in TZ. Additionally, relatively coarse particles, primarily from local sources, dominated dust depositions in both locations. The proportion of deposited dust particles with a size <20 μm was slightly higher in XT than that in TZ. Specifically, dust depositions in XT contained finer local dust particles and more dust from long-distance transport. Furthermore, D and Q had a significantly positive linear correlation in XT; however, no significant correlation was observed in TZ. Analysis of the wind dynamics and surface dust particle size indicated that topographic differences between the two stations caused these differences in correlation. [ABSTRACT FROM AUTHOR]

The business environment is increasingly uncertain due to the rapid development of disruptive information technologies, the changing global economy, and the COVID-19 pandemic. This brings great uncertainties to investors to predict the performance changes and risks of companies. This research proposes a sequential data-based framework that aggregates data from multiple sources including both structured and unstructured data to predict the performance changes. It leverages data generated from the early risk warning system in China stock market to measure and predict organization performance changes based on the risk warning status changes of public companies. Different from the models in existing literature that focus on the prediction of risk warning of companies, our framework predicts a portfolio of organization performance changes, including business decline and recovery, thus helping investors to not only predict public company risks, but also discover investment opportunities. By incorporating sequential data, our framework achieves 92.3% macro-F1 value on real-world data from listed companies in China, outperforming other static models. [ABSTRACT FROM AUTHOR]

Background. EF-hand domain-containing protein D2 (EFHD2) has recently been reported to participate in initiation of cancer. More evidence indicates that EFHD2 plays an important role in tumors, but the pan-cancer analysis of EFHD2 is still very limited. Methods. In this study, we downloaded the original mRNA expression data and SNP data of 33 kinds of tumor data. The gene expression data of different tissues were downloaded from the GTEX database, combined with TCGA data and corrected to calculate the difference of gene expression. The data of total survival time (OS) and progression-free survival (PFS) of TCGA patients were downloaded from the Xena database to further survey the relationship between the EFHD2 expression and prognosis. The CIBERSORT algorithm was used to analyze the RNA-seq data of 33 kinds of cancer patients in different subgroups. In this study, NCI-60 drug sensitivity data and RNA-seq data were downloaded to explore the relationship between genes and common antineoplastic drug sensitivity through correlation analysis. In this study, GSEA analysis was carried out from the Molecular Signature database through the packages of "clusterprofiler" and "enrichplot." By comparing the differences of signal pathways between high and low gene expression groups, the possible molecular mechanism of prognostic differences among 33 kinds of tumors was determined. Results. Our results indicated that EFHD2 was highly expressed in 23 kinds of tumors. In addition, EFHD2 was associated with stage in many kinds of tumors. The expression of EFHD2 was closely related to the OS of 12 kinds of cancer patients. In addition, Kaplan-Meier- (KM-) plot survival analysis indicated that the high expression of EFHD2 was related to the poor OS of 5 kinds of cancer, and the expression of EFHD2 was closely related to the PFI of 5 kinds of cancer patients. The expression of EFHD2 was closely related to immune infiltration, among which 18 cancers were significantly correlated with CD8T cells, 14 cancers were significantly correlated with T regulatory (Tregs) cells, 15 cancers were significantly correlated with CD4 memory activated Tcells, and EFHD2 was significantly correlated with common tumor-related regulatory genes such as TGF beta signaling, TNFA signaling, hypoxia, scorch death, DNA repair, autophagy, and iron death-related genes. The expression level of EFHD2 was significantly correlated with each tumor of TMB, including STAD, SARC, ACC, THYM, KICH, THCA, and TGCT. In MSI, there were significant differences in THYM, STAD, THCA, and TGCT. We used the CellMiner database to explore the sensitivity between EFHD2 gene and common antineoplastic drugs and found that the prediction of high expression of EFHD2 was related to the resistance of many antineoplastic drugs. In renal cell carcinoma, the high expression of EFHD2 is mainly concentrated in ALLOGRAFT_REJECTION, REACTIVE_OXYGEN_SPECIES_PATHWAY, INTERFERON_GAMMA_RESPONSE, IL6_JAK_STAT3_SIGNALING, INTERFERON_ALPHA_RESPONSE, and other signal pathways. GO results showed that the genes were mainly enriched in response to interferon-gamma, antigen processing and presentation, cellular response to interferon-gamma, and other pathways. KEGG results demonstrated that EFHD2 was mainly rich in phagosome, Epstein-Barr virus infection, Staphylococcus aureus infection, and other pathways. The results of Kaplan-Meier survival analysis demonstrated that the high expression of EFHD2 was significantly related to the poor prognosis. Conclusion. Our findings highlight the predictive value of EFHD2 in cancer and provide a potential research direction for elucidating the role of EFHD2 in tumorigenesis and drug resistance. [ABSTRACT FROM AUTHOR]

In a DC microgrid, the anti‐interference ability of bus voltage can easily become vulnerable due to low inertia caused by a large number of power electronic converters. This has already challenged the system stability and practicability in DC girds. In this study, a flexible virtual capacitance (FVC) control strategy with multiple constraints (MCs) is proposed to guarantee the security and stability of the DC microgrid, where the system stability, dynamic characteristics, actual operation requirements and realisability are considered as MCs. The FVC controller, which mainly modifies the reference current in voltage source converter by flexibly regulating virtual capacitance value, is given to provide inertial support and improve voltage quality. The stable operating boundary calculation based on small signal model and key control parameters design are further introduced into the control loop. This can ameliorate the dynamic response of the microgrid and obtain the better engineering practicability. Finally, experiment tests on a controller‐level hardware‐in‐the‐loop simulation platform are carried out to verify the validity of the proposed strategy. [ABSTRACT FROM AUTHOR]

Polybrominated biphenyl ethers (PBDEs) are a group of persistent organic pollutants with endocrine-disrupting, neurotoxic, tumorigenic and DNA-damaging activities. They are hydroxylated by human liver microsomal CYP enzymes, however, their mutagenicity remains unknown. In this study, 2,2′,4,4′-tetrabromobiphenyl ether (BDE-47, relatively abundant in human tissues) was investigated for micronuclei induction and DNA damage in mammalian cells. The results indicated that BDE-47 up to 80 μM under a 6 h/18 h (exposure/recovery, covering 2 cell cycles) regime did not induce micronuclei in V79-Mz and V79-derived cell lines expressing human CYP1A1 or 1A2, while it was moderately positive in human CYP2B6-, 2E1-and 3A4-expressing cell lines (V79-hCYP2B6, V79-hCYP2E1-hSULT1A1 and V79-hCYP3A4-hOR, respectively). Following 24 h exposure, BDE-47 induced micronuclei in V79-hCYP2E1-hSULT1A1 and V79-hCYP3A4 cells at increased potencies. In the human hepatoma (HepG2) cells BDE-47 (48 h exposure) was inactive up to 40 μM, however, pretreatment of the cells with ethanol (0.2%, v:v, inducer of CYP2E1) or rifampicin (10 μM, inducer of CYP3A4) led to significant micronuclei formation by BDE-47; pretreatment with bisphenol AF (100 nM) also potentiated BDE-47-induced micronuclei formation (which was blocked by a CYP2E1 inhibitor trans -1,2-dichloroethylene or a CYP3A inhibitor (ketoconazole). Immunofluorescent staining of centromere protein B with the micronuclei formed by BDE-47 in HepG2 cells pretreated with ethanol or rifampicin demonstrated selective formation of centromere-containing micronuclei. The increased phosphorylation of both histones H2AX and H3 in HepG2 by BDE-47 also indicated an aneugenic potential. Therefore, this study suggests that BDE-47 is an aneugen activated by several human CYP enzymes. [Display omitted] • 2,2′,4,4′-Tetrabromobiphenyl ether (BDE-47) is aneugenic in mammalian cells. • The aneugenicity of BDE-47 depends on metabolic activation by human CYP enzymes. • Human CYP3A4 and 2E1 might be the major enzymes activating BDE-47. [ABSTRACT FROM AUTHOR]

The Taklimakan Desert is a typical arid area. Due to the needs of production and life, a total of 2 km2 of constructed green land (hereinafter referred to as CGL) has been formed in the sand dune, resulting in the uniform underlying surface of the desert having been changed, which has led to the change in the near-surface energy distribution pattern and the formation of a local climate of the CGL that is obviously different from that of the desert climate. Therefore, it is necessary to study the varied interval of the threshold of meteorological factors and the regional climate characteristics of the CGL under the background of desert. The main results are as follows. Firstly, from sunrise to noon, the increasing rate of temperature in natural sandy land (hereafter, NSL) was higher than that in CGL, and the opposite results occurred between noon and sunset. The peak temperature of CGL was 2 h later than that of NSL. At night, the temperature at the boundary of the CGL was generally higher than that of the NSL and the central area of the CGL. In addition, the results show that under the combined influence of underlying conditions, local circulation and small terrain, the CGL (middle) daily range of temperature > NSL (west) > CGL (east) > CGL (west). The positive temperature change period of CGL was significantly shorter than that of NSL in all seasons. However, temperature inversion occurred at night in all seasons. The intensity of the temperature inversion was strongest in winter, with a maximum temperature difference of 12.8 °C, followed by autumn, spring, and summer, with a maximum difference of 6.4 °C. Secondly, the wind speed in the daytime was higher than that at night, and the wind speed in NSL was higher than that in CGL. In summer, if the average wind speed of the NSL was quantified as 1.0 m/s, the wind speed lapse rate reached 30% at the boundary of the CGL. Similarly, in the central area of CGL, the wind speed lapse rate reached 71%. [ABSTRACT FROM AUTHOR]

1-Methylpyrene (1-MP) is a ubiquitous environmental pollutant and rodent carcinogen. Its mutagenic activity depends on sequential activation by various CYP and sulfotransferase (SULT) enzymes. Previously we have observed induction of micronuclei and mitotic arrest by 1-MP in a Chinese hamster (V79)-derived cell line expressing both human CYP1A2 and SULT1A1 (V79-hCYP1A2-hSULT1A1), however, the mode of chromosome damage and the involvement of mitotic tubulin structures have not been clarified. In this study, we used immunofluorescent staining of centromere protein B (CENP-B) with the formed micronuclei, and that of β- and γ-tubulin reflecting the structures of mitotic spindle and centrioles, respectively, in V79-hCYP1A2-hSULT1A1 cells. The results indicated that 1-MP induced micronuclei in V79-hCYP1A2-hSULT1A1 cells from 0.125 to 2 μM under a 24 h/0 h (exposure/recovery) regime, while in the parental V79-Mz cells micronuclei were induced by 1-MP only at concentrations ≥ 8 μM; in both cases, the micronuclei induced by 1-MP were predominantly CENP-B positive. Following 54 h of exposure, 1-MP induced mitotic spindle non-congression and centrosome amplification (multipolar mitosis) in V79-hCYP1A2-hSULT1A1 cells, and anaphase/telophase retardation, at concentrations ≥ 0.125 μM with concentration-dependence; while in V79-Mz cells it was inactive up to 8 μM. This study suggests that in mammalian cells proficient in activating enzymes 1-MP may induce chromosome loss and mitotic disturbance, probably by interfering with the mitotic spindle and centrioles. 1-Methylpyrene is potently aneugenic in metabolism-proficient mammalian cells. 1-Methylpyrene may disrupt mitotic apparatus by interfering with tubulin. 1-Methylpyrene may retard mitotic progression. [ABSTRACT FROM AUTHOR]

Nanodiamonds are metastable allotropes of carbon. Based on their high hardness, chemical inertness, high thermal conductivity, and wide bandgap, nanodiamonds are widely used in energy and engineering applications in the form of coatings, such as mechanical processing, nuclear engineering, semiconductors, etc., particularly focusing on the reinforcement in mechanical performance, corrosion resistance, heat transfer, and electrical behavior. In mechanical performance, nanodiamond coatings can elevate hardness and wear resistance, improve the efficiency of mechanical components, and concomitantly reduce friction, diminish maintenance costs, particularly under high-load conditions. Concerning chemical inertness and corrosion resistance, nanodiamond coatings are gradually becoming the preferred manufacturing material or surface modification material for equipment in harsh environments. As for heat transfer, the extremely high coefficient of thermal conductivity of nanodiamond coatings makes them one of the main surface modification materials for heat exchange equipment. The increase of nucleation sites results in excellent performance of nanodiamond coatings during the boiling heat transfer stage. Additionally, concerning electrical properties, nanodiamond coatings elevate the efficiency of solar cells and fuel cells, and great performance in electrochemical and electrocatalytic is found. This article will briefly describe the application and mechanism analysis of nanodiamonds in the above-mentioned fields., (© 2024 Wiley‐VCH GmbH.)

To avoid the unexpected aggregation and reduce the cytotoxicity of nanomaterials as optical probes in cell imaging applications, we propose a programmed DNA-cube as a carrier for silver nanoparticles (Ag NPs) to construct a specific hydrogen sulfide (H 2 S) responsive platform (Ag NP@DNA-cube) for diagnosing colorectal cancer (CRC) in this study. The DNA-cube maintains good dispersion of Ag NPs while providing excellent biocompatibility. Based on the characteristic overexpression of endogenous H 2 S in CRC cells, the Ag NPs are etched by H 2 S within target cells into silver sulfide quantum dots, thereby selectively illuminating the target cells. The Ag NP@DNA-cube exhibits a specific fluorescence response to CRC cells and achieves satisfactory imaging.

Bisphenol (BP) compounds are endocrine-disrupting organic pollutants. BPs may increase the messenger RNA (mRNA) transcripts of nuclear receptors (NRs) regulating the expression of xenobiotic-metabolizing cytochrome P450 (CYP) enzymes. Their impact on the genotoxicity of metabolically activated carcinogens, however, remains unknown. In this study, effects of the bisphenols A, F, S, and AF on the expression of the aryl hydrocarbon receptor (AhR), the pregnane X receptor (PXR), the constitutive androstane receptor, and individual xenobiotic-metabolizing CYP enzymes in a human hepatoma (HepG2) cell line were investigated, along with in silico binding studies of BPs to each receptor. The results indicated that each BP at 1 to 100 nM concentrations increased the mRNA transcripts and protein levels of AhR, PXR, CYP1A1, 1A2, 1B1, 2E1, and 3A4. The predicted affinities of the BPs for binding AhR were comparable to those of potent agonists. Pretreatment of HepG2 cells with each BP potentiated the induction of micronuclei by benzo[ a ]pyrene, aflatoxin B1, benzene, and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone; this effect was abolished/reduced by inhibitors of NRs and/or CYPs. Our study suggests that BPs at human exposure levels may aggravate chromosome damage by several impactful carcinogens in human cells by inducing relevant CYP enzymes, mostly via NR modulation.

Bisphenols (BPs) are environmental pollutants with relevant DNA damage in human population; however, they are generally inactive in standard mutagenicity assays, possibly due to insufficient metabolic activation. In this study, induction of micronuclei and double-strand DNA breaks by BPA, BPF, and BPS in Chinese hamster V79-derived cell lines expressing various human CYP enzymes and a human hepatoma (C3A) (metabolism-proficient) cell line were investigated. Molecular docking of BPs to human CYPs indicated some substrate-enzyme potentials, including CYP1A1 for each compound, which did not induce micronuclei in V79-derived cell lines expressing human CYP1A2, 2E1, or 3A4 but became positive in human CYP1A1-expressing (V79-hCYP1A1) cells. In V79-hCYP1A1 and C3A cells, all compounds induced double-strand DNA breaks and micronuclei formation, which were blocked/significantly attenuated by 1-aminobenzotriazole (CYP inhibitor) or 7-hydroxyflavone (selective CYP1A1 inhibitor). Coexposure of C3A cells to pentachlorophenol (sulfotransferase 1 inhibitor) or ketoconazole (UDP-glucuronosyltransferase 1A inhibitor) potentiated micronuclei induction by each compound, with thresholds lowered from 2.5-5.0 to 0.6-1.2 μM. Immunofluorescence staining of centromere protein B with micronuclei formed in C3A cells by each compound indicated pure clastogenic effects. In conclusion, BPs are potently clastogenic in mammalian cells, which require activation primarily by human CYP1A1 and are negatively modulated by phase II metabolism.