7 results on '"Wang, Meiqiao"'
Search Results
2. In Vitro Diagnostic Assay to Detect SARS-CoV-2-Neutralizing Antibody in Patient Sera Using Engineered ACE-2 Mini-Protein.
- Author
-
Pereira de Jesus, Bruna Andersen, Gomes, Anderson Albino, Clark, Alex E., Rodrigues, Tayse Andrade, Ledgerwood-Lee, Melissa, Van Zant, Westley, Brickner, Howard, Wang, Meiqiao, Blum, David L., Cassera, Maria B., Carlin, Aaron F., Aronoff-Spencer, Eliah S., da Silva, Gustavo Felippe, Magalhães, Maria de Lourdes Borba, and Ray, Partha
- Subjects
IMMUNOGLOBULINS ,SARS-CoV-2 ,CELL receptors ,MEDICAL personnel - Abstract
The recent development and mass administration of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) vaccines allowed for disease control, reducing hospitalizations and mortality. Most of these vaccines target the SARS-CoV-2 Spike (S) protein antigens, culminating with the production of neutralizing antibodies (NAbs) that disrupt the attachment of the virus to ACE2 receptors on the host cells. However, several studies demonstrated that the NAbs typically rise within a few weeks after vaccination but quickly reduce months later. Thus, multiple booster administration is recommended, leading to vaccination hesitancy in many populations. Detecting serum anti-SARS-CoV-2 NAbs can instruct patients and healthcare providers on correct booster strategies. Several in vitro diagnostics kits are available; however, their high cost impairs the mass NAbs diagnostic testing. Recently, we engineered an ACE2 mimetic that interacts with the Receptor Binding Domain (RBD) of the SARS-2 S protein. Here we present the use of this engineered mini-protein (p-deface2 mut) to develop a detection assay to measure NAbs in patient sera using a competitive ELISA assay. Serum samples from twenty-one patients were tested. Nine samples (42.8%) tested positive, and twelve (57.1%) tested negative for neutralizing sera. The data correlated with the result from the standard commercial assay that uses human ACE2 protein. This confirmed that p-deface2 mut could replace human ACE2 in ELISA assays. Using bacterially expressed p-deface2 mut protein is cost-effective and may allow mass SARS-CoV-2 NAbs detection, especially in low-income countries where economical diagnostic testing is crucial. Such information will help providers decide when a booster is required, reducing risks of reinfection and preventing the administration before it is medically necessary. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
3. Switching Adaptive Controller for the Nonlinear Systems With Uncertainties From Unknown Powers.
- Author
-
Wang, Meiqiao, Liu, Yungang, and Man, Yongchao
- Subjects
- *
NONLINEAR systems , *UNCERTAIN systems , *CLOSED loop systems , *STATE feedback (Feedback control systems) , *PROGRAMMABLE controllers , *INTEGRATORS - Abstract
This paper considers the global stabilization for a class of uncertain nonlinear systems with unknown powers, unknown control directions, and unknown nonlinearities. Mainly due to the presence of the unknown powers which have not known upper bound, no existing methods are applicable to the control problem to be solved. In this paper, to compensate the serious system uncertainties and particularly to overcome the major obstruction from unknown powers, we appeal to the mechanism of switching adaptive feedback. By flexibly combining the domination and the method of adding a power integrator, we propose a new adaptive controller design, whose design parameters are tuned online based on a switching logic. The designed controller will become operative as long as finite switchings happen and will not lead to Zeno phenomenon, and can guarantee the global boundedness as well as ultimate convergence of the resulting closed-loop system. Two numerical examples are given to demonstrate the effectiveness of the developed design scheme. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
4. TlR2 and TlR4 are involved in the treatment of rheumatoid arthritis synovial fibroblasts with a medicated serum of asarinin through inhibition of Th1/Th17 cytokines.
- Author
-
Dai, Qiaomei, Li, Yaozhang, Wang, Meiqiao, Li, Yang, and Li, Ji
- Subjects
RHEUMATOID arthritis ,T helper cells ,FIBROBLASTS ,HIGH performance liquid chromatography ,SERUM - Abstract
Asarinin is one of the main active chemical components isolated from Xixin, a Chinese medicine. To investigate the role of asarinin in rheumatoid arthritis (RA), the present study investigated the effect of an asarinin-medicated serum on human fibroblast-like synoviocytes in vitro. An asarinin-medicated serum was generated and analyzed by high-performance liquid chromatography. Fibroblast-like synoviocytes were isolated from patients with osteoarthritis and RA. The third generation of the rheumatoid synoviocytes was used in the experimental research and the third generation of osteoarthritic synoviocytes was used as control cells. Trypan blue staining was performed to detect the viability of RA synovial fibroblasts (RASFs). ELISA, reverse transcription-quantitative (RT-q) PCR and western blotting were also performed to detect the expression of various cytokines. Additionally, RT-qPCR was employed to detect Toll-like receptor (TLR) 2 and TLR4. The results revealed that medicated asarinin serum inhibited the viability of RASFs in a dose- and time-dependent manner. The serum also suppressed the expression of interleukin (IL)-17A, tumor necrosis factor-α, interferon-γ, IL-6, TLR2 and TLR4. The inhibitory effect of asarinin drug serum on RASFs may be achieved by inhibition of T helper cell (T
h )1/Th 17 cytokines through suppression of TLR2 and TLR4. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF
5. Distributed adaptive control for nonlinear multi-agent systems with nonlinear parametric uncertainties.
- Author
-
Wang M and Li W
- Abstract
This paper considers the distributed tracking control problem for a class of nonlinear multi-agent systems with nonlinearly parameterized control coefficients and inherent nonlinearities. The essential of multi-agent systems makes it difficult to directly generalize the existing works for single nonlinearly parameterized systems with uncontrollable unstable linearization to the case in this paper. To dominate the inherent nonlinearities and nonlinear parametric uncertainties, a powerful distributed adaptive tracking control is presented by combing the algebra graph theory with the distributed backstepping method, which guarantees that all the closed-loop system signals are global bounded while the range of the tracking error between the follower's output and the leader's output can be tuned arbitrarily small. Finally, a numerical example is provided to verify the validity of the developed methods.
- Published
- 2023
- Full Text
- View/download PDF
6. TlR2 and TlR4 are involved in the treatment of rheumatoid arthritis synovial fibroblasts with a medicated serum of asarinin through inhibition of T h 1/T h 17 cytokines.
- Author
-
Dai Q, Li Y, Wang M, Li Y, and Li J
- Abstract
Asarinin is one of the main active chemical components isolated from Xixin, a Chinese medicine. To investigate the role of asarinin in rheumatoid arthritis (RA), the present study investigated the effect of an asarinin-medicated serum on human fibroblast-like synoviocytes in vitro . An asarinin-medicated serum was generated and analyzed by high-performance liquid chromatography. Fibroblast-like synoviocytes were isolated from patients with osteoarthritis and RA. The third generation of the rheumatoid synoviocytes was used in the experimental research and the third generation of osteoarthritic synoviocytes was used as control cells. Trypan blue staining was performed to detect the viability of RA synovial fibroblasts (RASFs). ELISA, reverse transcription-quantitative (RT-q) PCR and western blotting were also performed to detect the expression of various cytokines. Additionally, RT-qPCR was employed to detect Toll-like receptor (TLR) 2 and TLR4. The results revealed that medicated asarinin serum inhibited the viability of RASFs in a dose- and time-dependent manner. The serum also suppressed the expression of interleukin (IL)-17A, tumor necrosis factor-α, interferon-γ, IL-6, TLR2 and TLR4. The inhibitory effect of asarinin drug serum on RASFs may be achieved by inhibition of T helper cell (T
h )1/Th 17 cytokines through suppression of TLR2 and TLR4., (Copyright: © Dai et al.)- Published
- 2020
- Full Text
- View/download PDF
7. Amelioration of CIA by Asarinin Is Associated to a Downregulation of TLR9/NF-κB and Regulation of Th1/Th2/Treg Expression.
- Author
-
Dai Q, Wang M, Li Y, and Li J
- Subjects
- Animals, Anti-Inflammatory Agents pharmacology, Arthritis, Experimental immunology, Arthritis, Experimental pathology, Cytokines immunology, Dioxoles pharmacology, Down-Regulation drug effects, Knee Joint drug effects, Knee Joint pathology, Lignans pharmacology, Male, Mice, Inbred DBA, NF-kappa B immunology, T-Lymphocytes, Regulatory drug effects, T-Lymphocytes, Regulatory immunology, Th1 Cells drug effects, Th1 Cells immunology, Th2 Cells drug effects, Th2 Cells immunology, Toll-Like Receptor 9 immunology, Anti-Inflammatory Agents therapeutic use, Arthritis, Experimental drug therapy, Dioxoles therapeutic use, Lignans therapeutic use
- Abstract
To study the role of asarinin on collagen-induced arthritis (CIA) and its treatment mechanism on dendritic cells (DCs) and T cells. Before the onset of arthritis, asarinin were given orally to CIA mouse. Macroscopic scoring and micrometer caliper measurement were used to assess arthritis. The occurrence of cartilage destruction and bone erosion were assessed by histology of knee. Sandwich enzyme-linked immunosorbent assay (ELISA) and PCR were used to assess the level of cytokines in hindpaw and arthritic joint. The CD11c MicroBeads were employed to isolate CD11c+ cells from the spleen. Quantitative PCR was used to determine DCs surface molecules of spleen. Macroscopic score and the frequency of arthritis were inhibited by asarinin. Swelling of hindpaws, inflammatory cell infiltration in the synovium, cartilage destruction, and bone erosion were delayed with asarinin. Asarinin treatment suppressed the expression of T helper type 1 (Th1) cytokines and increased the levels of Th2 cytokines (interleukin (IL)-10), transforming growth factor (TGF)-β and Foxp3 in the synovium and hindpaw, however T-bet mRNA levels in synovium decreased. Lower expression of toll-like receptor 9 (TLR9) and nuclear factor-kappaB (NF-κB) were found in DCs after asarinin treatment. There was no difference in the expression of intercellular cell adhesion molecule-1(ICAM-1), OX40-L, and 4-1BBL in spleen DCs between the asarinin group and model control group. Asarinin can treat CIA. TLR9/NF-κB pathway may be involved in the asarinin treatment of CIA by skewing the balance of Th1/Th2/regulatory T (Treg) to a Th2 type.
- Published
- 2019
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.