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Teaching about biodiversity and its conservation could be an effective way to teach the importance of different species and human dependence on ecological support systems. This study compares the effectiveness of a digital and a paper-based dichotomous identification key for teaching pre-service teachers about plant species diversity. Twenty-four Slovenian teachers in pre-service training used a digital version of the key on tablet computers and twenty-nine used a paper-based version to identify woody species in the garden near their university faculty. Both keys contained the same species, identical photographs and the same sequence of steps to identify the species. The accuracy and time taken to identify the five species were measured. The participants also completed a questionnaire about the usability of the identification key. Overall, both versions of the key were equally good for determining species names. The digital version of the key was found to be more time-effective than the paper version only when multiple identification steps were required to identify species. The results confirm that those who scored better on the identification tasks have a better general opinion about the usability of the key; especially students using digital version of the key. Implications for teachers using or creating identification keys are discussed in the conclusion.

We run stochastic simulations of the spatial version of the rock-paper-scissors game, considering that individuals use sensory abilities to scan the environment to detect the presence of enemies. If the local dangerousness level is above a tolerable threshold, individuals aggregate instead of moving randomly on the lattice. We study the impact of the locally adaptive aggregation on the organisms' spatial organisation by measuring the characteristic length scale of the spatial domains occupied by organisms of a single species. Our results reveal that aggregation is beneficial if triggered when the local density of opponents does not exceed 30%; otherwise, the behavioural strategy may harm individuals by increasing the average death risk. We show that if organisms can perceive further distances, they can accurately scan and interpret the signals from the neighbourhood, maximising the effects of the locally adaptive aggregation on the death risk. Finally, we show that the locally adaptive aggregation behaviour promotes biodiversity independently of the organism's mobility. The coexistence probability rises if organisms join conspecifics, even in the presence of a small number of enemies. We verify that our conclusions hold for more complex systems by simulating the generalised rock-paper-scissors models with five and seven species. Our discoveries may be helpful to ecologists in understanding systems where organisms' self-defence behaviour adapts to local environmental cues., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Antipredator behavior is a self-preservation strategy present in many biological systems, where individuals join the effort in a collective reaction to avoid being caught by an approaching predator. We study a nonhierarchical tritrophic system, whose predator-prey interactions are described by the rock-paper-scissors game rules. We perform a set of spatial stochastic simulations where organisms of one out of the species can resist predation in a collective strategy. The drop in predation capacity is local, which means that each predator faces a particular opposition depending on the prey group size surrounding it. Considering that the interference in a predator action depends on the prey's physical and cognitive ability, we explore the role of a conditioning factor that indicates the fraction of the species apt to perform the antipredator strategy. Because of the local unbalancing of the cyclic predator-prey interactions, departed spatial domains mainly occupied by a single species emerge. Unlike the rock-paper-scissors model with a weak species because of a nonlocal reason, our findings show that if the predation probability of one species is reduced because individuals face local antipredator response, the species does not predominate. Instead, the local unbalancing of the rock-paper-scissors model results in the prevalence of the weak species' prey. Finally, the outcomes show that local unevenness may jeopardize biodiversity, with the coexistence being more threatened for high mobility.

Recognizing surrounding situations, such as enemy attacks, which can be realized by predator-prey relationships, is one of the common behaviors of the population in ecosystems. In this paper, we explore the relationship between such species' behavior and biodiversity in the spatial rock-paper-scissors game by employing the ecological concept "vigilance." In order to describe the vigilance process, we adopt a multiplex structure where two distinct layers describe virtual and physical interactions. By investigating the process of evolution in species, we also found that species with different vigilance go together. In addition, by utilizing the dynamic time warping method, we found that species with the same vigilance have consistent behavior, but species with different vigilance have diverse behavior. Our findings may lead to broader interpretations of mechanisms promoting biodiversity via vigilance in species ecosystems.

This study characterized the microfauna community during treatment of pulp and paper wastewater in a UNOX system aerated with pure oxygen, and with a high organic loading rate (0.4±0.06kg BOD/kg MLSSday), low sludge retention time (3.73±0.33 day), and high oxygen concentration (≤20mg O 2 /L) in comparison to municipal treatment systems. In the aeration tank, temperatures were high, averaging 35.7°C (March-May), then 38.9°C (June-August). Effluent quality was acceptable: 180±22mg COD/L, 7.2±2.1mg BOD 5 /L, and 33±5mg TSS/L. At 35.7°C, 5 taxa were identified in the activated sludge: small flagellates (flagellates <20μm), attached ciliates (Vorticella infusionum, Vorticella octava), crawling ciliates (Chilodonella uncinata) and free-swimming ciliates (Sathrophilus muscorum). During this period, the SBI (Sludge Biotic Index) was 6-7, corresponding to Quality Class II. At 38.9°C, two taxa co-dominated (Vorticella infusionum, Sathrophilus muscorum). When Vorticella infusionum dominated, the SBI was 5 or 7 (Quality Class III/II); when Sathrophilus muscorum dominated, the SBI was 0 (Quality Class IV). Slight changes in the abundance of two opposing Madoni keygroups impeded proper classification of activated sludge. However, effluent quality remained the same, showing that these indicators of activated sludge quality do not always reflect effluent quality., (Copyright © 2017 Elsevier GmbH. All rights reserved.)

Papers including articles that are produced because of the activities of LifeWatch ERIC, in the context of its second implementation period (2022 - 2026) and through the implementation of its new Strategic Working Plan, are published in this special collection. The articles include data papers, papers describing the development and functioning of analytical services and papers describing any other research outcome, produced either by LifeWatch ERIC or by any collaboration with any other ERIC, Research Infrastructure, global aggregator or other legal entity.

Paper mills processing recycled paper suffer from biofouling causing problems both in the mill and final product. The total bacterial community composition and identification of specific taxa in the process water and biofilms at the stock preparation and paper machine areas in a mill with recycled paper pulp was described by using a DNA-based approach. Process water in a similar mill was also analyzed to investigate if general trends can be found between mills and over time. Bacterial community profiles, analyzed by terminal-restriction fragment length polymorphism (T-RFLP), in process water showed that the dominant peaks in the profiles were similar between the two mills, although the overall composition was unique for each mill. When comparing process water and biofilm at different locations within one of the mills, we observed a separation according to location and sample type, with the biofilm from the paper machine being most different. 16S rRNA gene clone libraries were generated and 404 clones were screened by RFLP analysis. Grouping of RFLP patterns confirmed that the biofilm from the paper machine was most different. A total of 99 clones representing all RFLP patterns were analyzed, resulting in sequences recovered from nine bacterial phyla, including two candidate phyla. Bacteroidetes represented 45% and Actinobacteria 23% of all the clones. Sequences with similarity to organisms implicated in biofouling, like Chryseobacterium spp. and Brevundimonas spp., were recovered from all samples even though the mill had no process problems during sampling, suggesting that they are part of the natural paper mill community. Moreover, many sequences showed little homology to as yet uncultivated bacteria implying that paper mills are interesting for isolation of new organisms, as well as for bioprospecting.

The rock-paper-scissors (RPS) game is known as one of the simplest cyclic dominance models. This game is key to understanding biodiversity. Three species, rock (R), paper (P) and scissors (S), can coexist in nature. In the present paper, we first present a metapopulation model for RPS game with mutation. Only mutation from R to S is allowed. The total population consists of spatially separated patches, and the mutation occurs in particular patches. We present reaction-diffusion equations which have two terms: reaction and migration terms. The former represents the RPS game with mutation, while the latter corresponds to random walk. The basic equations are solved analytically and numerically. It is found that the mutation induces one of three phases: the stable coexistence of three species, the stable phase of two species, and a single-species phase. The phase transitions among three phases occur by varying the mutation rate. We find the conditions for coexistence are largely changed depending on metapopulation models. We also find that the mutation induces different paradoxes in different patches., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Formation of microbial biofilms and slimes is a general and serious problem in the operation of paper machines. Studies of microbial populations in paper machine-derived biofilms have been conducted using standard microbiological procedures; however, the bacterial genera present in this type of samples as well as their diversity are quite poorly known. Here, the bacterial diversity of 38 process water and 22 biofilm samples from four different Finnish paper machines were analyzed by length heterogeneity analysis of PCR-amplified 16S ribosomal DNA (LH-PCR). In addition, sequencing of the amplified 16S rRNA gene from 69 clones was conducted for characterization of the bacterial genera present in biofilm and slime samples. The LH-PCR profiles of both the free-living (process waters) and immobilized (biofilms) bacteria were diverse at all stages of the papermaking process. Out of the 69 sequenced clones, 44 belonged to alpha-Proteobacteria, most of which were close to the nitrogen-fixing root nodule genera Sinorhizobium, Rhizobium and Azorhizobium. Other clones were assigned to beta- and gamma-Proteobacteria and the phylum Bacteroidetes. In addition, eight of the clones were assigned to a yet uncultivated phylum, TM7. Finally, epifluorescence microscopy revealed that Gram-negative bacteria were predominant in both the biofilm (65%) and process water (54%) samples and a small coccoid cell morphology was most common in all samples. Together, our results show that the analysis of microbial samples from paper machines using modern molecular biology techniques adds valuable information and should, therefore, be useful as a more specific and sensitive microbiological method for the paper industry. This information could further be applied, e.g., in the development of more specific and environmental friendly antimicrobial agents for paper mills.

Miller & Wiens (2017) claim that low marine as compared with terrestrial diversity results from more frequent extinctions and insufficient time for diversification in marine clades. Their data on marine amniotes are unrepresentative of marine diversity, their analysis of clade dynamics is flawed, and they ignore previously proposed explanations for the diversity difference., (© 2018 John Wiley & Sons Ltd/CNRS.)

Plant genome sequences catalogue genes and the genetic elements that regulate their expression. Such inventories further research aims as diverse as mapping the molecular basis of trait diversity in domesticated plants or inquiries into the origin of evolutionary innovations in flowering plants millions of years ago. The transformative technological progress of DNA sequencing in the past two decades has enabled researchers to sequence ever more genomes with greater ease. Pangenomes - complete sequences of multiple individuals of a species or higher taxonomic unit - have now entered the geneticists' toolkit. The genomes of crop plants and their wild relatives are being studied with translational applications in breeding in mind. But pangenomes are applicable also in ecological and evolutionary studies, as they help classify and monitor biodiversity across the tree of life, deepen our understanding of how plant species diverged and show how plants adapt to changing environments or new selection pressures exerted by human beings., (© 2024. Springer Nature Limited.)

Environmental DNA (eDNA) monitoring, a rapidly advancing technique for assessing biodiversity and ecosystem health, offers a noninvasive approach for detecting and quantifying species from various environmental samples. In this review, a comprehensive overview of current eDNA collection and detection technologies is provided, emphasizing the necessity for standardization and automation in aquatic ecological monitoring. Furthermore, the intricacies of water bodies, from streams to the deep sea, and the associated challenges they pose for eDNA capture and analysis are explored. The paper delineates three primary eDNA survey methods, namely, bringing back water, bringing back filters, and bringing back data, each with specific advantages and constraints in terms of labor, transport, and data acquisition. Additionally, innovations in eDNA sampling equipment, including autonomous drones, subsurface samplers, and in-situ filtration devices, and their applications in monitoring diverse taxa are discussed. Moreover, recent advancements in species-specific detection and eDNA metabarcoding are addressed, highlighting the integration of novel techniques such as CRISPR-Cas and nanopore sequencing that enable precise and rapid detection of biodiversity. The implications of environmental RNA and epigenetic modifications are considered for future applications in providing nuanced ecological data. Lastly, the review stresses the critical role of standardization and automation in enhancing data consistency and comparability for robust long-term biomonitoring. We propose that the amalgamation of these technologies represents a paradigm shift in ecological monitoring, aligning with the urgent call for biodiversity conservation and sustainable management of aquatic ecosystems., (© 2024. Science China Press.)

The European Union Biodiversity Strategy 2030 (EUBDS) aims to regain biodiversity through enhanced forest conservation and protection, which may lead to increased timber harvest in non-EU countries. We aimed to identify the potential leakage of biodiversity risks as induced by the EUBDS. We created an indicator framework that allows one to quantify vulnerability of forest biodiversity. The framework is based on 26 biodiversity indicators for which indicator values were publicly available. We weighted single indicator values with countrywise modeled data on changed timber production under EUBDS implementation. Nearly 80% of the indicators pointed to higher vulnerability in the affected non-EU countries. Roundwood production was transferred to countries with, on average, lower governance quality (p = 0.0001), political awareness (p = 0.548), forest coverage (p = 0.034), and biomass (p = 0.272) and with less sustainable forest management (p = 0.044 and p = 0.028). These countries had more natural habitats (p = 0.039) and intact forest landscapes (p = 0.0001) but higher risk of species extinction (p = 0.006) and less protected area (p = 0.0001) than the EU countries. Only a few indicators pointed to lower vulnerability and biodiversity risks outside the EU. Safeguards are needed to ensure that implementation of EUBDS does not cause harm to ecosystems elsewhere. The EU regulation on deforestation-free supply chains might have limited effects because the sustainable management of existing and even expanding forests is not well considered. Sustained roundwood production in the EU is needed to avoid placing more pressure on more vulnerable ecosystems elsewhere. Decreasing species and habitat indicator values nevertheless call for global conservation and protection schemes. The EUBDS helped pave the way to the Kunming-Montreal Biodiversity Framework. Yet, lower values for the indicators mean governance and biodiversity engagement in non-EU countries suggest that this global framework might not sufficiently prevent leakage of risks to biodiversity. Effective land-use planning is necessary to balance conservation schemes with roundwood production., (© 2024 The Authors. Conservation Biology published by Wiley Periodicals LLC on behalf of Society for Conservation Biology.)

Climate changes may affect ecosystems destabilising relationships among species. We investigate the spatial rock-paper-scissors models with a regional unevenness that reduces the selection capacity of organisms of one species. Our results show that the regionally weak species predominates in the local ecosystem, while spiral patterns appear far from the region, where individuals of every species play the rock-paper-scissors game with the same strength. Because the weak species controls all local territory, it is attractive for the other species to enter the local ecosystem to conquer the territory. However, our stochastic simulations show that the transitory waves formed when organisms of the strong species reach the region are quickly destroyed because of local strength unbalance in the selection game rules. Computing the effect of the topology on population dynamics, we find that the prevalence of the weak species becomes more significant if the transition of the selection capacity to the area of uneven rock-paper-scissors rules is smooth. Finally, our findings show that the biodiversity loss due to the arising of regional unevenness is minimised if the transition to the region where the cyclic game is unbalanced is abrupt. Our results may be helpful to biologists in comprehending the consequences of changes in the environmental conditions on species coexistence and spatial patterns in complex systems.

Global species extinction rates are orders of magnitude above the background rate documented in the fossil record. However, recent data syntheses have found mixed evidence for patterns of net species loss at local spatial scales. For example, two recent data meta-analyses have found that species richness is decreasing in some locations and is increasing in others. When these trends are combined, these papers argued there has been no net change in species richness, and suggested this pattern is globally representative of biodiversity change at local scales. Here we reanalyze results of these data syntheses and outline why this conclusion is unfounded. First, we show the datasets collated for these syntheses are spatially biased and not representative of the spatial distribution of species richness or the distribution of many primary drivers of biodiversity change. This casts doubt that their results are representative of global patterns. Second, we argue that detecting the trend in local species richness is very difficult with short time series and can lead to biased estimates of change. Reanalyses of the data detected a signal of study duration on biodiversity change, indicating net biodiversity loss is most apparent in studies of longer duration. Third, estimates of species richness change can be biased if species gains during post-disturbance recovery are included without also including species losses that occurred during the disturbance. Net species gains or losses should be assessed with respect to common baselines or reference communities. Ultimately, we need a globally coordinated effort to monitor biodiversity so that we can estimate and attribute human impacts as causes of biodiversity change. A combination of technologies will be needed to produce regularly updated global datasets of local biodiversity change to guide future policy. At this time the conclusion that there is no net change in local species richness is not the consensus state of knowledge., (© 2016 by the Ecological Society of America.)

The rock-paper-scissors game is a paradigmatic model for biodiversity, with applications ranging from microbial populations to human societies. Research has shown, however, that mobility jeopardizes biodiversity by promoting the formation of spiral waves, especially if there is no conservation law in place for the total number of competing players. First, we show that even if such a conservation law applies, mobility still jeopardizes biodiversity in the spatial rock-paper-scissors game if only a small fraction of links of the square lattice is randomly rewired. Secondly, we show that zealots are very effective in taming the amplitude of oscillations that emerge due to mobility and/or interaction randomness, and this regardless of whether the later is quenched or annealed. While even a tiny fraction of zealots brings significant benefits, at 5% occupancy zealots practically destroy all oscillations regardless of the intensity of mobility, and regardless of the type and strength of randomness in the interaction structure. Interestingly, by annealed randomness the impact of zealots is qualitatively the same as by mobility, which highlights that fast diffusion does not necessarily destroy the coexistence of species, and that zealotry thus helps to recover the stable mean-field solution. Our results strengthen the important role of zealots in models of cyclic dominance, and they reveal fascinating evolutionary outcomes in structured populations that are a unique consequence of such uncompromising behavior.

The possibility of analyzing the general and functional diversity of the ecosystem is considered, as is unambiguous interpretation of the results obtained based on our work experience and previously obtained data. The prospects for studying small mammals without removal from the wild are discussed. We discuss distinguishing in a sample the resident component, which makes it possible to characterize local resource flows, and the nonresident component, dependent on the state of the territory as a whole. A description of the capture–mark–recapture protocol, methods for assessing spatial activity, calculating the density of the resident population per unit area, and the completeness of species richness estimate is provided. The estimates of animal numbers per unit of trapping effort in pooled sample, the population density of residents, and the index of the nonresident flow are compared. The possibility of analyzing the resource flows in "historical," "novel," and "hybrid" ecosystems, as well as using data to analyze the resilience of the ecosystem and detect the threshold point, is considered. [ABSTRACT FROM AUTHOR]

Biodiversity continues to decline despite protected area expansion and global conservation commitments. Biodiversity losses occur in existing protected areas, yet common methods used to select protected areas ignore postimplementation threats that reduce effectiveness. We developed a conservation planning framework that considers the ongoing anthropogenic threats within protected areas when selecting sites and the value of planning for costly threat-mitigating activities (i.e., enforcement) at the time of siting decisions. We applied the framework to a set of landscapes that contained the range of possible correlations between species richness and threat. Accounting for threats and implementing enforcement activities increased benefits from protected areas without increasing budgets. Threat information was valuable in conserving more species per spending level even without enforcement, especially on landscapes with randomly distributed threats. Benefits from including threat information and enforcement were greatest when human threats peaked in areas of high species richness and were lowest where human threats were negatively associated with species richness. Because acquiring information on threats and using threat-mitigating activities are costly, our findings can guide decision-makers regarding the settings in which to pursue these planning steps., (© 2023 Society for Conservation Biology.)

Native biodiversity loss and invasions by nonindigenous species (NIS) have massively altered ecosystems worldwide, but trajectories of taxonomic and functional reorganization remain poorly understood due to the scarcity of long-term data. Where ecological time series are available, their temporal coverage is often shorter than the history of anthropogenic changes, posing the risk of drawing misleading conclusions on systems' current states and future development. Focusing on the Eastern Mediterranean Sea, a region affected by massive biological invasions and the largest climate change-driven collapse of native marine biodiversity ever documented, we followed the taxonomic and functional evolution of an emerging "novel ecosystem", using a unique dataset on shelled mollusks sampled in 2005-2022 on the Israeli shelf. To quantify the alteration of observed assemblages relative to historical times, we also analyzed decades- to centuries-old ecological baselines reconstructed from radiometrically dated death assemblages, time-averaged accumulations of shells on the seafloor that constitute natural archives of past community states. Against expectations, we found no major loss of native biodiversity in the past two decades, suggesting that its collapse had occurred even earlier than 2005. Instead, assemblage taxonomic and functional richness increased, reflecting the diversification of NIS whose trait structure was, and has remained, different from the native one. The comparison with the death assemblage, however, revealed that modern assemblages are taxonomically and functionally much impoverished compared to historical communities. This implies that NIS did not compensate for the functional loss of native taxa, and that even the most complete observational dataset available for the region represents a shifted baseline that does not reflect the actual magnitude of anthropogenic changes. While highlighting the great value of observational time series, our results call for the integration of multiple information sources on past ecosystem states to better understand patterns of biodiversity loss in the Anthropocene., (Global Change Biology© 2024 The Authors. Global Change Biology published by John Wiley & Sons Ltd.)

Prompted by the increasing public focus on environmental policy and the continuous inability of States to reach environmental targets agreed upon in the context of the United Nations and the European Union, we explore the development of discourses within the Danish public agenda regarding nature management 2016-2021. This is done through a mixed-methods framework of discourse analysis and structural topic modeling based on documents from the Danish Parliament's Environmental committee 2016-2021, estimating topic prevalence, and analyzing the discourses within each topic, resulting in a qualitative overview of 21 identified topics and their associated discourses and an overview of how the different topic proportions changed over time. A shift in the public agenda was found: a change from discussions about untouched forest focused on trade-offs between timber extraction and biodiversity, to a discussion about different understandings of animal welfare in the context of large grazers in nature national parks in Denmark., (© 2023. The Author(s).)

The benthic biome of the deep-sea floor, one of the largest biomes on Earth, is dominated by diverse and highly productive heterotrophic protists, second only to prokaryotes in terms of biomass. Recent evidence suggests that these protists play a significant role in ocean biogeochemistry, representing an untapped source of knowledge. DNA metabarcoding and environmental sample sequencing have revealed that deep-sea abyssal protists exhibit high levels of specificity and diversity across local regions. This review aims to provide a comprehensive summary of the known heterotrophic protists from the deep-sea floor, their geographic distribution, and their interactions in terms of parasitism and predation. We offer an overview of the most abundant groups and discuss their potential ecological roles. We argue that the exploration of the biodiversity and species-specific features of these protists should be integrated into broader deep-sea research and assessments of how benthic biomes may respond to future environmental changes., (© 2024 The Authors. Environmental Microbiology published by Applied Microbiology International and John Wiley & Sons Ltd.)

The spatiotemporal arrangement of interacting populations often influences the maintenance of species diversity and is a subject of intense research. Here, we study the spatiotemporal patterns arising from the cyclic competition between three species in two dimensions. Inspired by recent experiments, we consider a generic metapopulation model comprising "rock-paper-scissors" interactions via dominance removal and replacement, reproduction, mutations, pair exchange, and hopping of individuals. By combining analytical and numerical methods, we obtain the model's phase diagram near its Hopf bifurcation and quantitatively characterize the properties of the spiraling patterns arising in each phase. The phases characterizing the cyclic competition away from the Hopf bifurcation (at low mutation rate) are also investigated. Our analytical approach relies on the careful analysis of the properties of the complex Ginzburg-Landau equation derived through a controlled (perturbative) multiscale expansion around the model's Hopf bifurcation. Our results allow us to clarify when spatial "rock-paper-scissors" competition leads to stable spiral waves and under which circumstances they are influenced by nonlinear mobility.

Feedback is critically important to student learning, but the reduced frequency of assignments combined with isolated or stand-alone tasks reduces the opportunity for students to engage with feedback and use it effectively to enhance their learning. Here, I evaluate student attainment during a module consisting of eight iterated tasks where the task itself is the same but the academic content differs. At the end of the module, students then self-assess their eight submissions and select two for summative assessment. I demonstrate that achievement increases over the course of the module, and that choice is valuable in allowing students to achieve higher summative marks for the course than their formative marks would suggest. Students who performed more weakly at the start of the module saw the greatest benefits from practice and choice, suggesting that these students particularly can benefit from repeated cycles of feedback and increase their marks.

Background and Objectives: The use of endophytic fungi for management of phenol residue in paper and pulp industries has been shown as cost-effective and eco-friendly approach. In this study, isolation of endophytic fungi from roots, stems, and leaves of Hibiscus sabdariffa was conducted. Additionally, the isolated fungi were examined for their ability to degrade phenol and its derivatives in paper and pulp industrial samples, using different growth conditions. Materials and Methods: Out of 35 isolated endophyitc fungi, 31 were examined for their phenol biodegradation capacity using Czapek Dox broth medium containing Catechol and Resorcinol as a sole carbon source at final concentrations of 0.4, 0.6 and 0.8%. Results: A total of 35 fungal species belonging to 18 fungal genera were isolated and identified from different parts of H. sabdariffa plants. All strains have the capability for degrading phenol and their derivatives with variable extents. The optimum condition of degrading phenol in paper and pulp effluent samples by Fusarium poae11r7 were at pH 3-5, temperature at 28-35°C, good agitation speed at no agitation and 100 rpm. Conclusion: All endophytic fungal species can utilize phenol and its derivatives as a carbon source and be the potential to degrade phenol in industrial contaminants.

The spatial rock-paper-scissors ecosystem, where three species interact cyclically, is a model example of how spatial structure can maintain biodiversity. We here consider such a system for a broad range of interaction rates. When one species grows very slowly, this species and its prey dominate the system by self-organizing into a labyrinthine configuration in which the third species propagates. The cluster size distributions of the two dominating species have heavy tails and the configuration is stabilized through a complex spatial feedback loop. We introduce a statistical measure that quantifies the amount of clustering in the spatial system by comparison with its mean-field approximation. Hereby, we are able to quantitatively explain how the labyrinthine configuration slows down the dynamics and stabilizes the system.

Anthropogenic alterations have paramount impacts on the alpha and beta diversity of aquatic resources, and fishes are predominantly susceptible to such impacts. Mahanadi River, one of the major peninsular rivers of India, has abundant fish resources, which play a significant role in supporting the fishers' livelihoods. The exploratory study in the river conducted for three consecutive years recorded 148 species under 53 families. Cyprinids dominated the fish diversity with 41 species, followed by Bagrids (9) and Sciaenids (7). One hundred-one species under 29 families were reported from the freshwater stretch. With a total of 111 species reported under 48 families, the estuarine and tidal freshwater stretch was more speciose, due to marine migrant species which advent the estuarine and tidal freshwaters stretch for breeding and feeding purposes. Tikarpara, a conserved site within a sanctuary, was the most species-diverse as well as a species-even site. The study also recorded the extension of the distributional range of 3 fish species and also 4 exotic species from the river. The seasonal variations in diversity indicated that the deviations were not prominent in freshwater sites, whereas in tidal brackish water sites, species richness was relatively higher in post-monsoon, and species evenness was higher during monsoon. Taxonomic distinctness test showed that the average taxonomic distinctness was high for tidal estuarine locations as they harbour taxonomically distant fishes. The hierarchical clustering of sites showed the inordinate effect of river gradient and fragmentation on the fish community structure. Analyzing the key drivers of the assemblage structure of the entire river, salinity was the major deterministic factor, and within the freshwater stretch, the major influences were depth, transparency, and specific conductivity. The study concluded that, despite all of its ecological stresses, Mahanadi still supports rich fish diversity, yet there is a notable shift in the fish community structure. There is a need for integrating molecular and morphological tools for the taxonomic revision of many genera and species for proper in situ and ex situ conservation measures and to formulate future biodiversity management plans addressing to reduce the impacts of the ecological threats., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Background: Free and open access to primary biodiversity data is essential for informed decision-making to achieve conservation of biodiversity and sustainable development. However, primary biodiversity data are neither easily accessible nor discoverable. Among several impediments, one is a lack of incentives to data publishers for publishing of their data resources. One such mechanism currently lacking is recognition through conventional scholarly publication of enriched metadata, which should ensure rapid discovery of 'fit-for-use' biodiversity data resources., Discussion: We review the state of the art of data discovery options and the mechanisms in place for incentivizing data publishers efforts towards easy, efficient and enhanced publishing, dissemination, sharing and re-use of biodiversity data. We propose the establishment of the 'biodiversity data paper' as one possible mechanism to offer scholarly recognition for efforts and investment by data publishers in authoring rich metadata and publishing them as citable academic papers. While detailing the benefits to data publishers, we describe the objectives, work flow and outcomes of the pilot project commissioned by the Global Biodiversity Information Facility in collaboration with scholarly publishers and pioneered by Pensoft Publishers through its journals Zookeys, PhytoKeys, MycoKeys, BioRisk, NeoBiota, Nature Conservation and the forthcoming Biodiversity Data Journal. We then debate further enhancements of the data paper beyond the pilot project and attempt to forecast the future uptake of data papers as an incentivization mechanism by the stakeholder communities., Conclusions: We believe that in addition to recognition for those involved in the data publishing enterprise, data papers will also expedite publishing of fit-for-use biodiversity data resources. However, uptake and establishment of the data paper as a potential mechanism of scholarly recognition requires a high degree of commitment and investment by the cross-sectional stakeholder communities.

In 2006, after a visual inspection of the Leonardo da Vinci's Atlantic Codex by a scholar, active molds were reported to have been present on Codex pages showing areas of staining. In the present paper, molecular methods were used to assess the current microbiological risk to stained pages of the manuscript. Bacterial and fungal communities were sampled by a non-invasive technique employing nitrocellulose membranes. Denaturing gradient gel electrophoresis of 16 S rRNA gene and internal transcribed space regions were carried out to study the structure of the bacterial and fungal communities and band patterns were analyzed by the multivariate technique of principal component analysis. Any relationship between the presence of an active microbial community and staining was excluded. The presence of potential biodeteriogens was evaluated by constructing bacterial and fungal clone libraries, analyzing them by an operational taxonomic unit (OTU) approach. Among the bacteria, some OTUs were associated with species found on floors in clean room while others were identified with human skin contamination. Some fungal OTU representatives were potential biodeteriogens that, under proper thermo-hygrometric conditions, could grow. The retrieval of these potential biodeteriogens and microorganisms related to human skin suggests the need for a continuous and rigorous monitoring of the environmental conditions, and the need to improve handling procedures.

Cyclic dominance of species is a potential mechanism for maintaining biodiversity. The author investigates the generalised scenario when the cyclic dominance of three or more interacting species is described by a non-symmetric matrix game that has multiple Nash equilibria. Modified Lotka-Volterra equations are proposed to incorporate the effects of swarming, and the condition for biodiversity is derived. The species are modelled using replicator equations, where each member of the species is assigned a fitness value. The authors show, for the first time, that the 'swarming effect' has an important role to play in the maintenance of biodiversity. The authors have also discovered the existence of a critical value of the swarming parameter for a given mobility, above which there is a high probability of existence of biodiversity.

Biodiversity is essential to the viability of ecological systems. Species diversity in ecosystems is promoted by cyclic, non-hierarchical interactions among competing populations. Central features of such non-transitive relations are represented by the 'rock-paper-scissors' game, in which rock crushes scissors, scissors cut paper, and paper wraps rock. In combination with spatial dispersal of static populations, this type of competition results in the stable coexistence of all species and the long-term maintenance of biodiversity. However, population mobility is a central feature of real ecosystems: animals migrate, bacteria run and tumble. Here, we observe a critical influence of mobility on species diversity. When mobility exceeds a certain value, biodiversity is jeopardized and lost. In contrast, below this critical threshold all subpopulations coexist and an entanglement of travelling spiral waves forms in the course of time. We establish that this phenomenon is robust; it does not depend on the details of cyclic competition or spatial environment. These findings have important implications for maintenance and temporal development of ecological systems and are relevant for the formation and propagation of patterns in microbial populations or excitable media.

We examined the entanglement of biodiversity conservation, human-animal interactions, zootherapy, and local beliefs among Sumatran Healers and their local community by completing an ethnography of 43 Indigenous Healers across 8 tribes in Bengkulu Province, Sumatra, Indonesia. Data collection tools were interviews, observations, videos, photographs, and a researcher journal. Of the 43 Healers, 30 used animals and mentioned 62 species. Of the animals identified, the International Union for Conservation of Nature Red List lists 34% (n = 21) as endangered, decreasing, or vulnerable, including Sumatran tiger (Panthera tigris sumatrae), Sumatran elephant (Elephas maximus sumatranus), and Sumatran rhinoceros (Dicerorhinus sumatrensis). Of the 30 Healers using animals, 50% (n = 15) practiced healing with at least one endangered, decreasing, or vulnerable animal. We defined 3 personas: Healer self-persona, Healer-imposed persona, and community-imposed persona. A persona represented a group's opinions and sentiments related to Healers killing animals for medicinal purposes. Using an iterative data analysis process, we grouped the data across the 3 personas into 5 themes: ease of killing and preparing animals, emotions related to killing animals, animal value, relationship to religion, and Healers are tricksters. The complexity of merging the identities of Healers and the community within an actor-network embodies the relationality of actions, interactions, and feelings among Healers, between Healers and animals, and between Healers and the community. Conservationists should be cognizant of Healers' medicinal use of animals, views of human-animal interactions, and zootherapy from all social and emotional perspectives. The data led to defining Indigenous Healer ecological knowledge components of zootherapy, human-animal interactions, and biodiversity conservation., (© 2024 Society for Conservation Biology.)

The challenge of managing aquatic connectivity in a changing climate is exacerbated in the presence of additional anthropogenic stressors, social factors, and economic drivers. Here we discuss these issues in the context of structural and functional connectivity for aquatic biodiversity, specifically fish, in both the freshwater and marine realms. We posit that adaptive management strategies that consider shifting baselines and the socio-ecological implications of climate change will be required to achieve management objectives. The role of renewable energy expansion, particularly hydropower, is critically examined for its impact on connectivity. We advocate for strategic spatial planning that incorporates nature-positive solutions, ensuring climate mitigation efforts are harmonized with biodiversity conservation. We underscore the urgency of integrating robust scientific modelling with stakeholder values to define clear, adaptive management objectives. Finally, we call for innovative monitoring and predictive decision-making tools to navigate the uncertainties inherent in a changing climate, with the goal of ensuring the resilience and sustainability of aquatic ecosystems., (© 2024 His Majesty the King in Right of Canada, Crown Copyright, Institute of Marine Research and The Authors. Journal of Fish Biology published by John Wiley & Sons Ltd on behalf of Fisheries Society of the British Isles. Reproduced with the permission of the Minister of Fisheries and Oceans Canada. This article is published with the permission of the Controller of HMSO and the King's Printer for Scotland.)