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In this study, fresh harvested high moisture wheat was placed in a simulated warehouse for ventilation drying experiment, and the moisture content change in each layer of the wheat grain pile, the wheat crude protein, wet gluten content, flour properties, falling value, etc. were measured. The results showed that the longitudinal ventilation technology could reduce the wheat moisture content to safe moisture content in a short time for the wheat with moisture content more than 20% in the simulated warehouse, and had no significant adverse effect on the processing quality of wheat. The use of ventilation technology not only reduced the wheat moisture content effectively, but also restrained the deterioration of high moisture wheat quality, providing a theoretical basis for the actual warehouse drying of high moisture wheat.

The complexation behaviors of pagoda[n]arenes (n = 4, 5) with ferrocene, ferrocenium and analogues cobaltocenium were studied. The inclusion complexes of pagoda[n]arenes can protect ferrocenium from oxidation in organic solvents and improve the stability and oxygen resistance of ferrocenium. [ABSTRACT FROM AUTHOR]

Light harvesting is one of the fundamental steps in photosynthesis, and developing artificial light harvesting systems (LHSs) with high energy transfer efficiency is still a challenging task. Herein, we designed and constructed a novel fluorescent tetragonal Pd(II)-metallacycle (DDPH-Pd) as a new platform for the construction of artificial LHSs. 6,15-bis(pyridine)-9,12-bis(dodecyloxy)-substituted benzo[5]helicene (DDPH) and ethylenediamine palladium nitrate were coordinated to generate self-assembled DDPH-Pd. Carmosine and Nile red were chosen as acceptors due to their good spectral overlap with that of DDPH-Pd , which is essential for fabricating light-harvesting system. The artificial LHSs show good energy transfer efficiency (61%) and antenna efficiency (5.0) in Carmosine@ DDPH-Pd with a 50:1 donor/acceptor ratio. [Display omitted] • The new fluorescent metallacycles is assembled by the bipyridine ligand DDPH and ethylene-diamine palladium nitrate. • Amphipathic metallacycles further aggregate in water to form nano spheres. • The Pd(II)-metallacycles are used as a new platform for the construction of artificial light harvesting systems. • LHSs have good energy transfer efficiency (61%) and antenna efficiency (5.0) with donor/acceptor ratio of 50:1. [ABSTRACT FROM AUTHOR]

Background: Given the poor prognosis of lung squamous cell carcinoma (LUSC), the aim of this study was to screen for new prognostic biomarkers., Methods: The TGCA&#95;LUSC dataset was used as the training set, and GSE73403 was used as the validation set. The genes involved in necroptosis-related pathways were acquired from the KEGG database, and the differential genes between the LUSC and normal samples were identified using the GSEA. A necroptosis signature was constructed by survival analysis, and its correlation with patient prognosis and clinical features was evaluated. The molecular characteristics and drug response associated with the necroptosis signature were also identified. The drug candidates were then validated at the cellular level., Results: The TCGA&#95;LUSC dataset included 51 normal samples and 502 LUSC samples. The GSE73403 dataset included 69 samples. 159 genes involved in necroptosis pathways were acquired from the KEGG database, of which most showed significant differences between two groups in terms of genomic, transcriptional and methylation alterations. In particular, CHMP4C, IL1B, JAK1, PYGB and TNFRSF10B were significantly associated with the survival ( p < 0.05) and were used to construct the necroptosis signature, which showed significant correlation with patient prognosis and clinical features in univariate and multivariate analyses ( p < 0.05). Furthermore, CHMP4C, IL1B, JAK1 and PYGB were identified as potential targets of trametinib, selumetinib, SCH772984, PD 325901 and dasatinib. Finally, knockdown of these genes in LUSC cells increased chemosensitivity to those drugs., Conclusion: We identified a necroptosis signature in LUSC that can predict prognosis and identify patients who can benefit from targeted therapies.

Herein, host-guest complexation between pagoda[ n ]arenes ( n = 4, P4 ; n = 5, P5 ) and tropylium cation ( G ) was investigated in detail. It was found that both P4 and P5 showed surprisingly strong binding affinities toward the tropylium cation with association constants of more than 10 7 M -1 for the 1:1 host-guest complexes. The theoretical calculations showed different host-guest complexion ways for complexes G @ P4 and G @ P5 and the strong π···π interactions and multiple C-H···π interactions play a very important role in the formation of these stable complexes, respectively. Moreover, the switchable processes of guest binding and release in the complexes can be effectively controlled by redox stimuli, and they can be also visible by the color and fluorescence changes.

Background: Spindle and kinetochore-associated complex subunits 1-3 (SKA1-3) stabilize the kinetochore-attached spindle microtubules in metaphase. Due to the dysregulation in multiple cancers, SKA1-3 is considered a predictor for the prognosis of the patients. However, the potential clinical applications of SKA1-3, particularly in hepatocellular carcinoma (HCC) prognosis and progression, have completely unknown yet., Methods: For the analysis of SKA1-3 expression and applications in clinics in HCC patients, several databases, such as STRING, UALCAN, GEO, and TCGA, were searched. In addition, the underlying mechanisms of SKA for the regulation of HCC occurrence, development, and progression were also explored., Results: Compared to the normal controls, HCC patients showed dramatically elevated SKA1-3 expression at the mRNA level, and the values of the area under the curve (AUC) were 0.982, 0.887, and 0.973, respectively. Increased SKA1-3 expression levels were associated with the clinical stage, age, body mass index, tumor grade, tissue subtype, and Tp53 mutation status in HCC patients. The analyses of Kyoto Encyclopedia of Genes and Genome (KEGG) and Gene ontology (GO) demonstrated that SKA1-3 are enriched mainly in the Fanconi anemia, homologous recombination, spliceosome, DNA replication, and cell cycle signaling pathways. The hub genes, such as CDK1, CCNB1, CCNA2, TOP2A, BUB1, AURKB, CCNB2, BUB1B, NCAPG , and KIF11 , were identified in protein-protein interactions (PPIs). The expression levels of hub genes were increased in HCC patients and predictive of a poor prognosis. Finally, the expression levels of SKA1-3 were determined using the GEO database., Conclusions: SKA1-3 are potential prognostic biomarkers of and targets for HCC. In addition, SKA1-3 may affect HCC prognosis via the Fanconi anemia pathway, homologous recombination, spliceosome, DNA replication, and cell cycle signaling pathway., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Song, He, Ji, Duan, Zhang and Hu.)

Gecko ( Gekko japonicus ) extracts have been used in traditional Chinese medicine for many years. It has been proven that the gecko polypeptide mixture (GPM) extracted from gecko can inhibit the growth of multiple types of tumor cells. In order to investigate the possible anti-tumor molecular mechanisms of GPM, we used RNA-seq technology to identify the differentially expressed genes (DEGs) of human hepatocellular carcinoma (HCC) HepG2 cells treated with or without GPM. MTT assay was used to detect the viability of HepG2 cells. DAPI fluorescence staining was performed to observe morphological changes in the nuclei of HepG2 cells. Western blot analysis was applied to observe the expressions of apoptosis-related and endoplasmic reticulum stress (ERS)-related proteins in HepG2 cells. Flow cytometry assay was performed to detect the apoptosis and reactive oxygen species (ROS) in HepG2 cells. Our results showed that GPM inhibited HepG2 cells proliferation and induced the apoptosis of HepG2 cells. RNA-seq analysis suggested that the ER-nucleus signaling pathway involved in the anti-cancer molecular mechanism of GPM. Therefore, GPM may induce apoptosis in HepG2 cells via the ERs pathway., Competing Interests: Competing Interests: The authors have declared that no competing interest exists.