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The escalating levels of carbon dioxide in flue gas and the resultant greenhouse effect have heightened the need for adsorbents with high CO 2 sorption and separation capabilities. However, Traditional heated synthesis methods are energy-intensive and often involve toxic solvents, which are not viable for widespread industrial use. This study introduces a room-temperature, eco-friendly synthesis technique to produce UiO-66-(OH) 2 with varying amounts of PVP (0/0.1/0.2 g), effectively adjusting the particle morphology. Results confirm that this method maintains robust CO 2 adsorption and selectivity. Notably, the addition of 0.1 g of PVP led to a reduction in pore size and adsorption capacity of CO 2 , yet remarkably enhanced its CO 2 /N 2 separation performance sixfold, outperforming traditional CO 2 adsorbents. Dynamic breakthrough experiments further validated the effectiveness of UiO-66-(OH) 2 in separating binary mixtures of CO 2 /N 2 (v/v = 15/85). The separation performance of UiO-66-(OH) 2 on CO 2 /N 2 was significantly improved after the addition of PVP at room temperature. [Display omitted] • OH-functionalized UiO-66 has been successfully synthesized using green room temperature technology. • The addition of PVP can effectively prevent the agglomeration of UiO-66-(OH) 2 , forming dispersed and uniform particles. • UiO-66-(OH) 2 maintains robust CO 2 adsorption and selectivity, which was synthesized at room temperature. • The CO 2 /N 2 separation of UiO-66-(OH) 2 with the addition of PVP increased to 724. [ABSTRACT FROM AUTHOR]

[Display omitted] In this work, we present an effective approach for promoting the immobilization of anionic dyes on the collagen fibers of the leather matrix via introducing layered double oxide (LDO), which is obtained by calcining layered double hydroxides (LDH), inspired by incorporating their memory effect and charge effect. The results indicate that the calcination increases specific surface area, oxygen vacancies, and Al3+ defects of LDH nanosheets, and the structure of LDH nanosheets can be reconstructed by rehydration. Diffusion behavior of both LDH and LDO nanosheets into the collagen fibers follows the Langmuir model. The LDO nanosheets can penetrate into the collagen fibers more easily and evenly than that of the LDH nanosheets. Moreover, the formation of ionic bonds, hydrogen bonds, and coordination bonds between the nanosheets and the collagen stabilizing the collagen microstructures can endow the collagen fibers with improved thermal stability. Increased porosity of the collagen fibers results in enhanced adsorption and immobilization capacity for anionic dyes on the collagen fibers of the leather matrix in leather post-tanning process. Furthermore, adsorption behavior of anionic dye on the collagen fibers can be well accorded with pseudo-second-order and Langmuir model, exhibiting a monolayer adsorption process. This established cooperative approach will be helpful to extend the application of clay for improving the dyeing performance of leather matrix towards eco-leather manufacture and effectively reduce emission of dyes from the source in leather manufacturing. [ABSTRACT FROM AUTHOR]

Numerous efforts have been made in last decade on fully Automated Operational Modal Analysis (AOMA) with field measurement. Considerable user interaction is required especially when parametric system identification methods are involved with Stability Diagrams (SD) for the modal identification. This paper proposes an improved procedure with multi-stage clustering to address this issue. It is generally applicable to any method that relies on SD for the modal identification. The proposed clustering framework offers advantages over traditional AOMA with reduced reliance on the threshold setting in the hierarchical clustering. It relies on hierarchical clustering to identify only one cluster center. This cluster center is then used as input for Max-min distance clustering to achieve adaptive clustering. The proposed Modal Evaluation Index can be employed to eliminate the effect of subjective bias to small dimensional clusters and to assess the validity of each representative mode identified from the cluster. Measured datasets from the Z24 bridge benchmark and the Yingxian wooden pagoda serve to illustrate the performance and effectiveness of this automation strategy. [ABSTRACT FROM AUTHOR]

Toxic Microcystis blooms are widespread in aquatic bodies, posing major threats to aquatic and human life. Recently, bioflocculants have attracted considerable attention as a promising biomaterial for Microcystis management. In search of a novel organism that can produce an efficient bioflocculant for controlling harmful algae sustainably, the native gastropod Cipangopaludina chinensis was co-cultured continuously with toxic Microcystis under different initial algal cell densities. The bioflocculation effect of snail mucus on toxic Microcystis , microcystin removal, and toxin accumulation in snails was investigated. In addition, the properties of the adhesive mucus were characterized using microscopic, X-ray diffraction, infrared spectroscopy, and polysaccharide and proteome analyses. Microcystis cells were captured and flocculated by the snail mucus; removal efficiencies of up to 89.9% and 84.8% were achieved for microalgae and microcystin-leucine arginine (MC-LR), respectively, when co-cultured with C. chinensis for only one day. After nine-day exposure, less than 5.49 µg/kg DW microcystins accumulated in the snails, indicating safety for human consumption. The snail mucus contained 104.3 µg/mg protein and 72.7 µg/mg carbohydrate, which provide several functional groups beneficial for Microcystis bioflocculation. The main monosaccharide subunits of polysaccharides are galactose, galactosamine, glucosamine, fucose, glucose, and mannose. Most of them are key components of polysaccharides in many bioflocculants. Gene Ontology analysis indicated the protein enrichment in binding processes and catalytic activity, which may account for Microcystis bioflocculation via protein binding or enzymatic reactions. The findings indicate that native C. chinensis secretes adhesive mucus that can act as bioflocculant for toxic Microcystis from ambient water and can be an effective and eco-friendly tool for Microcystis suppression. [Display omitted] • Bioflocculants are a promising biomaterial for Microcystis management. • Cipangopaludina chinensis secretes adhesive mucus that can act as bioflocculant. • Effective bioflocculation may be because of active functional groups. • Snail mucus captured and flocculated Microcystis cells, achieving up to 89.9% removal efficiency. [ABSTRACT FROM AUTHOR]

In light of the documented elevated concentrations of total mercury (Hg) and methylmercury (MeHg) in poultry originating from Hg-contaminated sites, a knowledge gap persists regarding the levels of Hg found in home-produced eggs (HPEs) and the associated dietary exposure risks in regions affected by Hg mining. To address this knowledge gap, a comprehensive investigation was undertaken with the primary objectives of ascertaining the concentrations of THg and MeHg in HPEs and evaluating the potential hazards associated with the consumption of eggs from the Wanshan Hg mining area in Southwest China. The results showed that THg concentrations in HPEs varied within a range of 10.5–809 ng/g (with a geometric mean (GM) of 64.1 ± 2.7 ng/g), whereas MeHg levels spanned from 1.3 to 291 ng/g (GM, 23.1 ± 3.4 ng/g). Remarkably, in half of all eggs, as well as those collected from regions significantly impacted by mining activities, THg concentrations exceeded the permissible maximum allowable value for fresh eggs (50 ng/g). Consumption of these eggs resulted in increased exposure risks associated with THg and MeHg, with GM values ranging from 0.024 to 0.17 µg/kg BW/day and 0.0089–0.066 µg/kg BW/day, respectively. Notably, the most substantial daily dosage was observed among children aged 2–3 years. The study found that consuming HPEs could result in a significant IQ reduction of 34.0 points for the whole mining area in a year. These findings highlight the potential exposure risk, particularly concerning MeHg, stemming from the consumption of local HPEs by residents in mining areas, thereby warranting serious consideration within the framework of Hg exposure risk assessment in mining locales. [Display omitted] • Elevated THg and MeHg concentrations were detected in HPEs from Hg mining areas. • Nearly half of the HPEs samples exhibited higher THg concentrations than 50 ng/g. • HPEs is a significant pathway for human exposure to MeHg in mining areas. • HPEs eating can cause certain intelligence impairment in children in mining areas. [ABSTRACT FROM AUTHOR]

The Acipenseriformes, as one of the earliest extant vertebrates, plays an important role in the evolution of fishes and even the whole vertebrates. Here we collected and analyzed all complete mitochondrial genomes of Acipenseriformes species. Phylogenetic analyses demonstrated that the polytomous branch included Acipenseridae and Polyodontidae formed five clades. The Polyodontidae clade and the Scaphirhynchus clade both were monophyletic group, whereas the Acipenser species and the Huso species both were polyphyletic group. The Bayesian divergence times showed that the origin time for Acipenseriformes was at 318.0 Mya, which was similar to the some previous results of 312.1 Mya, 346.9 Mya and 389.7 Mya. The result was in good consistent with the paleontological data available and the split time of the Pacific and Atlantic Oceans from the Jurassic to the Cretaceous (Laurasia splits in North America and Eurasia). The dN/dS ratios showed the evolutionary rates gradually slow down in five major Acipenseriformes clades from the Clade A (the Pacific sturgeons species) to Clade C (the genus Scaphirhynchus), which was related to the process of geographical formation. • The Polyodontidae and the Scaphirhynchus were monophyletic group, whereas the Acipenser species and the Huso species both were polyphyletic group. • The origin time for Acipenseriformes was at 318.0 Mya, which was in good consistent with the paleontological data available and the split time of the Pacific and Atlantic Oceans from the Jurassic to the Cretaceous. • The evolutionary rates of the Acipenseriformes was slow down from the Clade A to the Clade C gradually, which was consistent with the process of geographical formation. [ABSTRACT FROM AUTHOR]

Doxorubicin (DOX) is a potent anti-neoplastic agent with cumulative cardiotoxicity. DOX-induced cardiotoxicity has been shown to depend on the different dosing times. However, the basis for determining the dosing time to minimize DOX-induced cardiotoxicity and the underlying mechanisms remain incompletely understood. Here we first showed that SIRT3, the major mitochondrial deacetylase, is negatively correlated to DOX-induced cardiotoxicity through the regulation of ATP production, mitochondrial membrane potential (MMP) level and ROS level in human pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Then, we used in vivo experiments to demonstrate that DOX significantly reduced the SIRT3 expression and the SIRT3 activity as reflected by the increased AcK68MnSOD/MnSOD ratio in rats after six weeks of treatment. Notably, the activity of SIRT3 had an obvious diurnal rhythm pattern in the myocardium of healthy rats. More importantly, an obvious lower AcK68MnSOD/MnSOD ratio was observed in rat hearts with DOX administrated at Zeitgeber time (ZT) 9 (ZT 0 was the time lights were turned on) than ZT1, which represent the peak and trough of SIRT3 activity. Moreover, DOX ZT9 reduced the body weight loss, extended the survival period, improved the heart function and alleviated the myocardial lesions compared to DOX ZT1. Mechanistic investigations demonstrated that DOX ZT1 significantly reduced ATP production, oxygen consumption rate (OCR) at various respiration states, MMP level and MnSOD activity and enhanced the H 2 O 2 level compared with CON ZT1, whereas there was no significant effect for DOX ZT9 compared with CON ZT9. Taken together, dosing at the peak time of SIRT3 activity reduced DOX-induced cardiotoxicity, which may be related to the increased endogenous tolerance against the mitochondrial dysfunction and oxidative stress caused by DOX. • SIRT3 plays a critical role in doxorubicin-induced cardiotoxicity in hiPSC-CMs. • Dosing depending on SIRT3 activity attenuates doxorubicin-induced cardiotoxicity. • Dosing depending on SIRT3 activity renders tolerance against mitochondrial dysfunction and oxidative stress. [ABSTRACT FROM AUTHOR]

Bile acids receptor TGR5 and its agonist INT-777, which has been found to be involved in the NLRP3 inflammasome pathway, play an important role in inflammatory diseases. However, the role of INT-777 in acute pancreatitis (AP) has not been reported. In this present study, we found that TGR5 was expressed in pancreatic tissue and increased after AP onset induced by caerulein and further evaluated the impact of INT-777 on the severity of AP. The results showed that INT-777 could reduce the severity of AP in mice, which was manifested as decreased pancreatic tissue damage as well as the decrease of serum enzymes (amylase and lipase), pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) and the expression of necrosis related proteins (RIP3 and p-MLKL). Furthermore, we found that INT-777 reduced the reactive oxygen species (ROS) production in pancreatic acinar cells and inhibited the activation of NLRP3 inflammasome pathway. In conclusion, our data showed that INT-777 could protect pancreatic acinar cell against necrosis and reduce the severity of AP, which may be mediated by inhibiting ROS/NLRP3 inflammasome pathway. [ABSTRACT FROM AUTHOR]

Induced electric field (IEF), as an alternative non-conventional processing technique, is utilized to sterilize liquid foods. In this study, the survival and sublethal injury of S. aureus under IEF were investigated in 0.85% normal saline, and the inactivation mechanism of IEF was expounded. The plate count results showed that the sublethal injury rates remained above 90% after IEF treatment for more than 8.4 s, and 7.1 log CFU/mL of S. aureus was completely inactivated after 14 s IEF treatment. Scanning electron microscopy and transmission electron microscope images showed that IEF caused the destruction of cell membrane and internal substructure, and the damage to intracellular substructure was more severe. Altered membrane integrity or permeability was demonstrated through flow cytometry and confocal laser scanning microscope analysis, and the different damage to cells was quantified by propidium iodide & 5-carboxy fluorescein diacetate single and double staining. In addition, IEF treatment also decreased the membrane potential and esterase activity of S. aureus cells. Putative inactivation mechanism of IEF against S. aureus is a complex process, and its apoptosis is the result of the combination of several factors, which provide a basis for understanding the inactivation mechanism of IEF. [Display omitted] • Germicidal efficacy and inactivation mechanism of IEF on S. aureus were explored. • Sublethal injury rates remained above 90% after IEF treatment for more than 8.4 s. • IEF caused the destruction of cell membrane, substructure, and permeability. • IEF decreased the membrane potential and esterase activity of S. aureus cells. • Putative inactivation mechanism of IEF against S. aureus is a complex process. [ABSTRACT FROM AUTHOR]

[Display omitted] Aqueous gel supercapacitors, as an important component of flexible energy storage devices, have received widespread attention for their fast charging/discharging rates, long cycle life and high electrochemical stability under mechanical deformation condition. However, the low energy density of aqueous gel supercapacitors has greatly hindered their further development due to the narrow electrochemical window and limited energy storage capacity. Therefore, different metal cation-doped MnO 2 /carbon cloth-based flexible electrodes herein are prepared by constant voltage deposition and electrochemical oxidation in various saturated sulphate solutions. The influence of different metal cations as K+, Na+ and Li+ doping and deposition conditions on the apparent morphology, lattice structure and electrochemical properties are explored. Furthermore, the pseudo-capacitance ratio of the doped MnO 2 and the voltage expansion mechanism of the composite electrode are investigated. The specific capacitance and pseudo-capacitance ratio of the optimized δ-Na 0.31 MnO 2 /carbon cloth as MNC-2 electrode could be reached 327.55 F/g at 10 mV/s and 35.56% of the pseudo-capacitance, respectively. The flexible symmetric supercapacitors (NSCs) with desirable electrochemical performances in the operating range of 0–1.4 V are further assembled with MNC-2 as the electrodes. The energy density is 26.8 Wh/kg at the power density of 300 W/kg, while the energy density can still reach 19.1 Wh/kg when the power density is up to 1150 W/kg. The energy storage devices with high-performance developed in this work can provide new ideas and strategic support for the application in portable and wearable electronic devices. [ABSTRACT FROM AUTHOR]

UDP-glucuronosyltransferases (UGTs) are the primary phase II enzymes catalyzing the conjugation of glucuronic acid to the xenobiotics with polar groups for facilitating their clearance. The UGTs belong to a superfamily that consists of diverse isoforms possessing distinct but overlapping metabolic activity. The abnormality or deficiency of UGTs in vivo is highly associated with some diseases, efficacy and toxicity of drugs, and precisely therapeutic personality. Despite the great effects and fruitful results achieved, to date, the expression and functions of individual UGTs have not been well clarified, the inconsistency of UGTs is often observed in human and experimental animals, and the complex regulation factors affecting UGTs have not been systematically summarized. This article gives an overview of updated reports on UGTs involving the various regulatory factors in terms of the genetic, environmental, pathological, and physiological effects on the functioning of individual UGTs, in turn, the dysfunction of UGTs induced disease risk and endo- or xenobiotic metabolism-related toxicity. The complex cross-talk effect of UGTs with internal homeostasis is systematically summarized and discussed in detail, which would be of great importance for personalized precision medicine. [ABSTRACT FROM AUTHOR]

An experimental system based on the o-core transformer structure was established and used to extract pectin from orange peel waste. Unlike other electric-field-assisted techniques, it avoids the use of powered electrodes. The acidic solvent acts as the secondary coil connected to a purpose-made glass chamber which forms a closed loop. Then the induced electric field in the system appears to be under the influence of alternating magnetic flux based on Faraday's law of induction . We found that an increase in the excitation voltage causes enhancement of the pectin yield. Increasing frequency from 20 to 200 kHz had a negative impact on pectin yield partially due to the increased impedance of the primary coil. The ionic conduction was enhanced at the same excitation voltage since there are more free ions in the mixture at lower pH. This means that a lower impedance in the mixture is conducive to extraction. This multidisciplinary technique combines the transformer concept with the electric-field-assisted process, thus providing a reference for the application into agricultural by-products treatment. [ABSTRACT FROM AUTHOR]

Bacteria possess protective mechanisms against excess Mn(Ⅱ) to reduce its toxicity. Stenotrophomonas sp. MNB17 showed high Mn(Ⅱ) removal capacity (92.24–99.16 %) by forming Mn-precipitates (MnCO 3 and Mn-oxides), whose Mn-oxides content increased with increasing Mn(Ⅱ) concentrations (10–50 mM). Compared with 0 mM Mn(Ⅱ)-stressed cells, transcriptomic analysis identified genes with the same transcriptional trends in 10 mM and 50 mM Mn(Ⅱ)-stressed cells, including genes involved in metal transport, cell envelope homeostasis, and histidine biosynthesis, as well as genes with different transcriptional trends, such as those involved in oxidative stress response, glyoxylate cycle, electron transport, and protein metabolism. The upregulation of histidine biosynthesis and oxidative stress responses were the most prominent features of these metabolisms under Mn(Ⅱ) stress. We confirmed that the increased level of reactive oxygen species was one of the reasons for the increased Mn-oxides formation at high Mn(Ⅱ) concentrations. Metabolite analysis indicated that the enhanced histidine biosynthesis rather than the tricarboxylic acid cycle resulted in an elevated level of α-ketoglutarate, which helped eliminate reactive oxygen species. Consistent with these results, the exogenous addition of histidine significantly reduced the production of reactive oxygen species and Mn-oxides and enhanced the removal of Mn(Ⅱ) as MnCO 3. This study is the first to correlate histidine biosynthesis, reactive oxygen species, and Mn-oxides formation at high Mn(Ⅱ) concentrations, providing novel insights into the molecular regulatory mechanisms associated with Mn(Ⅱ) removal in bacteria. • Strain MNB17 strain handles increased Mn(Ⅱ) stress by producing more Mn-oxides. • Reactive oxygen species contribute to the Mn-oxides generation. • Mn(Ⅱ) stress induces significant upregulation of histidine biosynthesis in MNB17. • Strain MNB17 diverts histidine towards α-ketoglutarate to combat oxidative stress. • Exogenous histidine reduces Mn-oxides formation and promotes Mn(Ⅱ) removal as MnCO 3. [ABSTRACT FROM AUTHOR]

Phenanthrene (PHE) is one of the most abundant polycyclic aromatic hydrocarbon compounds (PAHs) in the aquatic environment. The loaches were exposed at concentrations of 0.30、1.00、3.00 mg L−1 for 60 days. The effects of PHE on the testis development were evaluated by calculating the survival rate, observing the structure of testis and analyzing transcriptome. Firstly, PHE markedly decreased the survival rate in a dose-dependent manner. Then, the number and density of spermatogonia, primary spermatocytes, secondary spermatocytes and spermatids were substantially reduced under PHE exposure. The space in the seminiferous tubule obviously increased in the high PHE concentration group. Meanwhile, transcriptome comparative analysis identified 5329 differentially expressed genes (DEGs) including 2928 up-regulated and 2401 down-regulated in the testis of loach exposed PHE for 60 days. Meiotic cell cycle, arganelle fission, ATPase activity and adenylate nucleotide binding were significantly differences by GO (Gene Ontology) enrichment. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis revealed that TNF (Tumor Necrosis Factor) signaling pathway, CAMs (Cell Adhesion Molecules), cytochrome P450 and lipid metabolism were markedly regulated. In addition, eight DEGs were randomly selected from the testis transcriptomics results for qPCR verification, the results were consistent with RNA-Seq. Finally, related genes (piwil2 , dmc1 , vasa , ubr2, dnd , rnf17, plcb2, c-fos, gpx4) of testis development were further confirmed and they were differentially regulated after PHE exposure. In summary, a survey of the mechanism of loach testis response to PHE was performed, and a large number of gene expression levels regarding metabolism, spermatogenesis and immunity genes were acquired from RNA-seq. This study provide informations for elucidating the molecular mechanism of PHE affected the testis development of loach. • PHE markedly decreased the survival rate in a dose-dependent manner. • The number and density of spermatogonia, primary spermatocytes, secondary spermatocytes and spermatids were substantially reduced under PHE exposure. • 5329 differentially expressed genes (DEGs) were identified in the RNA-seq. • Metabolic pathways and immune process were significantly enriched in DEGs. • Related genes of testis development were differentially regulated after PHE exposure. [ABSTRACT FROM AUTHOR]

The combination of a rotating perpendicular magnetic field and a flowing Ca 2+ solution accelerated the calcium uptake of fresh-cut apple cubes and shortened the time needed for calcium equilibration between the Ca 2+ solution and samples. The influence of operational parameters (Reynolds number of the flowing Ca 2+ solution, magnetic flux density of the magnetic field, rotating frequency of the magnetic field, and temperature) on the calcium uptake rate were investigated. Negative exponential models were used to sufficiently describe the calcium uptake rate of samples. Magnetic flux density and temperature positively affected the calcium uptake rate. Higher Reynolds number and rotating frequency both accelerated the calcium diffusion, but excessively high levels (turbulent flowing at Re = 4134 or rotating frequency at 10 Hz) decreased the calcium content. Neither the flowing Ca 2+ solution alone, nor the magnetic field alone, accelerated the calcium diffusion. This study provides an alternative methodology for the mineral fortification of fresh-cut fruits under the joint action of a rotating magnetic field and a flowing electrolyte solution. [ABSTRACT FROM AUTHOR]

A method for determining the total solids and fat content of liquid whole egg products based on the principle of transformer was developed. The electrical parameters (terminal voltage and λ value) in the secondary circuit, where the sample acted as the coil, were investigated under the action of alternating magnetic flux in the frequency range from 60 to 400 Hz. Findings indicated that subtle changes of quality indices could be detected by electrical parameters based on the transformer properties that relate to the amount of free ions in the samples. As the inductive voltage was fixed, terminal voltage was negatively correlated with quality indices of total solids and fat content. Therefore the decrement of impedance in the secondary circuit caused the increment of inductive current according to Ohm's law. Linear prediction models for the aforementioned quality indices based on these electrical parameters were also established. Judging from coefficients of determination ( R 2 ) and the root mean square error of prediction (RMSEP) of these models, the λ value was found to be a better predictor for quality indices of liquid whole egg products than terminal voltage. It is believed that this method has the potential to evaluate the quality of liquid food materials over a wider frequency and temperature range. [ABSTRACT FROM AUTHOR]

Potato protein (PP) was modified with laccase or tyrosinase to obtain covalent cross-linking structures, and then the blends of native/enzyme-treated protein and potato starch (PS) with different ratios were prepared. The FT-IR results suggested the interactions between protein and starch strengthened after enzyme treatment. The relative crystalline raised to 26–29% after the protein mixing with starch. The thermal degradation temperatures of the blends of enzyme-treated protein and PS (296.71–312.91 °C) were apparently lower than that of the blends of PP:PS (312.31–312.76 °C). Compared to the blends of PP:PS, the peak viscosity, trough viscosity and final viscosity of the blends of enzyme-treated protein and PS decreased by 12.7–41.1%. The blends of enzyme-treated protein and PS had a lower storage modulus than native blends. The outcome would provide a view on the theoretical basis of interactions between protein and starch in exploring favorable potato staple foods. •Covalent cross-linking structure in potato protein was formed by enzyme treatment. •The native/enzyme-treated protein was blended with potato starch with different ratios. •The blends of enzyme-treated protein and starch had lower thermostability. •The blends of enzyme-treated protein and starch had lower paste viscosity. •The blends of enzyme-treated protein and starch had lower storage and loss moduli. [ABSTRACT FROM AUTHOR]

The high detected frequencies of hexaconazole (Hex) and arsenic (As) increased the probabilities of their co-existence in agricultural products. However, the combined toxicity effect and mechanism of action for these two pollutants were still unclear. In this study, an untargeted metabolomics method with ultra high performance liquid chromatography and tandem mass spectrometry (UPLC-MS/MS) was developed to monitor the changes of endogenous metabolites and metabolism pathways in mice liver. Our study revealed that significant differences in metabolomics profiles were observed after Hex, As, and Hex+As exposure for 90 d. Hex exposure altered 54 metabolites and 11 pathways significantly which were mainly lipid-related. For As exposure, 63 metabolites and 9 pathways were affected most of which were amino acid-related. Hex+As induced 93 metabolites changes with 34% was lipids and lipid-like molecules and 22% was organic acids and derivatives. Hex+As exposure shared the pathways that altered by Hex and As indicated that the interaction of Hex and As might be independent action. The results of this study could provide an important insight for understanding the mechanism of combined toxicity for Hex and As and be helpful for evaluating their health risk to human. [Display omitted] • Hexaconazole exposure mainly altered lipid-related metabolites and pathways. • Arsenic exposure mainly changed amino acid-related metabolites and pathways. • Hexaconazole+arsenic shared most metabolites and pathways with hexaconazole or arsenic. • The interaction of hexaconazole and arsenic was independent action. [ABSTRACT FROM AUTHOR]

Ni-VN heterojunction grown on nickel foam is used for efficient electrolysis of water to produce hydrogen. [Display omitted] • The promotional effect of inert nitrides for metal catalysts in electrolysis was explored. • A facile approach was developed for synthesizing heterostructured Ni-VN catalysts. • The Ni-VN catalysts exhibited excellent alkaline HER performance exceeding that of Pt/C. • The electron transfer between Ni-VN interfaces was proved beneficial to the high activity. • DFT calculation revealed the high activity of Ni-VN interfaces for HER. The promotional effects of inert nitrides for metal catalysts in the electrolysis are rarely reported. Recently, we reported an efficient Ni-VN/NF (that NF represents Ni foam) composite by nitriding treatment of NiV-layered double hydroxides (NiV-LDH) precursor that was in-situ hydrothermal growth on nickel foam. The optimal Ni-VN/NF exhibited outstanding electrocatalytic performance for hydrogen evolution reaction (HER) with a small overpotential of 39 mV at 10 mA cm−2 and strong durability for 100 h without degradation. The optimized electronic structure and local charge density at the hetero-interface of Ni-VN, evidenced by both experiment and DFT results, were significantly modulated by the electron transfer from Ni to V-N bond at the interfaces, leading to moderate H* adsorption energy and diminished barrier for H 2 O dissociation, synergistically promoted basic HER. This work highlights the design principle of strong metal-nitride interactions for advanced HER catalysts. [ABSTRACT FROM AUTHOR]

The quorum-sensing (QS) system between the phages and their hosts is important for the phage lysis-lysogeny decision. In Vibrio cholerae , the QS system consists of a LuxR-type receptor VqmA (VqmA Vc) and an autoinducer molecule 3,5-dimethylpyrazin-2-ol (DPO). A VqmA homolog encoded by vibriophage VP882 (VqmA Phage) can intervene the host QS system via binding to both the host-produced DPO and its cognate promoter (P qtip) to induce the phage lysogeny-to-lysis transition, whereas VqmA Vc cannot influence the VqmA Phage -induced pathway, suggesting an asymmetry regulation. In this study, we report the crystal structure of VqmA Phage -DPO complex at 2.65 Å and reveal that the mechanism of DPO recognition is conserved in VqmA homologs. Besides, we identify a non-classical palindrome sequence in P qtip , which can be effectively recognized by VqmA Phage but not VqmA Vc. The sequence contains an interval longer than that in the vqmR promoter recognized by VqmA Vc. In addition, the two DBD regions in the VqmA Phage dimer exhibit more relaxed architecture than that of the reported VqmA Vc , which is likely to be in the conformation that may easily bind to target promoter containing a longer interval. In summary, our findings provide a structural and biochemical basis for the DBD-dependent DNA recognition in different promoter regions in the phage lysogeny-to-lysis decision communication system, and provide clues for developing phage therapies against Vibrio cholerae infection. • The crystal structure of VqmA Phage -DPO complex was solved. • The molecular mechanism of DPO recognition is conserved in VqmA homologs. • DBD domain of VqmA Phage is important in qtip promoter recognition. • The target qtip promoter region of VqmA Phage was identified. • Demonstrates the difference of DNA sites recognized by VqmA Phage and VqmA Vc. [ABSTRACT FROM AUTHOR]

Abstract The aim of this study was to compare effects of linear dextrans (backbones of α-(1 → 6) linkages) varying with weight-average molecular weights (Mw: T10, T70, T250, T750, T2000) on the quality of wheat breads. The firmness of fresh bread crumb was decreased with increasing dextran Mw and the highest Mw dextran (T2000) lowered the staling rate of stored breads. Dough rheology clarified that only the specific dextran (T70) apparently changed dough viscoelastic, strengthening the viscous behavior. Significant decreases in breakdown and setback viscosity of starch slurry were observed by addition of dextrans, especially dextran T2000. Analyses in the long-term retrogradation of starch and starch-gluten systems indicated that the promising antistaling influence of dextran T2000 was caused by either inhibitions of amylopectin retrogradation or beneficial variations induced by gluten. Positive effects of starch-gluten and dextran-gluten associations on amylopectin retrogradation were revealed. These results suggested that the highest Mw dextran (T2000) possessed the potential for increasing consumer acceptance of bread products. Highlights • Dextran T2000 decreased the formation of amylose crystallites. • Dextran T2000 inhibited amylopectin recrystallization. • Retardation of dextrans on the long-term retrogradation of amylopectin was highlighted by gluten. • Starch-gluten and dextran-gluten associations played a powerful role in amylopectin reordering. [ABSTRACT FROM AUTHOR]

Aims Phospholamban (PLB) regulates the cardiac Ca 2 + -ATPase (SERCA2a) in sarcoplasmic reticulum (SR). However, the localization of PLB at subcellular sites outside the SR and possible contributions to Ca 2 + cycling remain unknown. We examined the intracellular distribution of PLB and tested whether a pool of PLB exists in the nuclear envelope (NE) that might regulate perinuclear/nuclear Ca 2 + (nCa 2 + ) handling in cardiomyocytes (CMs). Methods and results Using confocal immunofluorescence microscopy and immunoblot analyses of CMs and CM nuclei, we discovered that PLB was highly concentrated in NE. Moreover, the ratio of PLB levels to SERCA levels was greater in NE than in SR. The increased levels of PLB in NE were a consistent finding using a range of antibodies, tissue samples, and species. To address a possible role in affecting Ca 2 + handling, we used Fluo-4 based confocal Ca 2 + imaging, with scan-lines across cytosol and nuclei, and evaluated the effects of PLB on cytosolic and nCa 2 + uptake and release in mouse CMs. In intact CMs, isoproterenol increased amplitude and decreased the decay time of Ca 2 + transients not only in cytosol but also in nuclear regions. In saponin-permeabilized mouse CMs ([Ca 2 + ] i = 400 nM), we measured spontaneous Ca 2 + waves after specific reversal of PLB activity by addition of the Fab fragment of an anti-PLB monoclonal antibody (100 μg/ml). This highly selective immunological reagent enhanced Ca 2 + uptake (faster decay times) and Ca 2 + release (greater intensity) in both cytosol and across the nuclear regions. Conclusions Besides SR, PLB is concentrated in NE of CMs, and may be involved in modulation of nCa 2 + dynamics. [ABSTRACT FROM AUTHOR]

Drosophila Zeste is a DNA binding protein important for chromatin-targeted regulation of gene expression. It is best studied in the context of transvection—a mechanism of interallelic gene regulation involving paired chromosomes—and repression of the expression of white by Zeste mutants. Both of these functions depend on the DNA binding and self-association properties of Zeste, but the underlying structural basis remains unknown. Here we report the crystal structure of the DNA binding domain of Zeste in complex with a 19-bp DNA duplex containing the consensus recognition sequence motif. The structure reveals a helix–turn–helix Myb/homeodomain-like fold with the Zeste-specific insertion sequence forming a short helix and a long loop. Direct base contacts by the major groove binding helix principally account for the sequence-specific recognition, and backbone contacts via the Zeste-specific insertion are mainly responsible for the length requirement and the orientation of DNA. Our structural and biochemical characterizations of the DNA binding property of Zeste uncover an altered DNA binding modality of homeodomain-like proteins, and the structural information should facilitate the unraveling of the intricate mechanism of Zeste in regulation of gene expression. [ABSTRACT FROM AUTHOR]

The effects of mung bean starch (MS) on physicochemical properties of rice flour (RF) and the quality of extruded rice noodles were investigated. Results showed that the amylose content, retrogradation degree, storage modulus (G′) and loss modulus (G″) increased gradually as addition levels of MS increased, while the solubility, swelling power and pasting viscosities decreased with increasing MS content. Acceptable cooking quality and textural properties in the extruded rice noodles were obtained when MS content was up to 5 g/100 g. Sensory evaluation results showed that the scores of firmness, elasticity, slipperiness and chewiness increased gradually as addition levels of MS increased. Scanning electron micrographs of raw extruded rice noodles showed that the surface was smoother and the network of cross-section was more compacted for noodles made from blends of 95 g/100 g RF and 5 g/100 g MS than those from pure RF. This study provides a crucial basis for understanding the interaction between MS and RF and the quality improvement in the extruded rice noodles. [ABSTRACT FROM AUTHOR]

Non-conventional heating technology based on electric fields can be utilized to process liquid foods. In this study, the induced electric field (IEF) was investigated to clarify its inactivation mechanism on E.coli. Staining results show that inactivation of E.coli by IEF can be attributed to the reversible destruction of the cell membrane, followed by the denaturation of intracellular enzymes, and finally the irreversible rupture of the cell membrane. The increased levels of extracellular proteins and nucleic acids were also observed. IEF treatment at 400 Hz and 800 V (or 53 V/cm) results in a reduction of 4.5 log CFU·mL−1 in the number of E.coli. Storage life analysis shows that IEF treatment can improve the stability of apple juice and the content of bioactive components. Thus, IEF is a potential technique for liquid food processing. • The inactivation mechanism of induced electric field on E.coli was investigated. • A reduction of 4.5 log CFU·mL−1 of E.coli was realized by applying the IEF. • IEF treatment retains bio-active components in fresh apple juice. • The storage stability of apple juice was improved through IEF treatment. [ABSTRACT FROM AUTHOR]

Halomonas spp. are moderately halophilic bacteria with the ability to tolerate various heavy metals. However, the role of basic cellular metabolism, particularly amino acid metabolism, has not been investigated in Halomonas spp. under excess Mn(Ⅱ). The strain Halomonas sp. MNB13 was isolated from a deep-sea ferromanganese nodule and can tolerate 80 mM Mn(Ⅱ). To comprehensively explore the mechanisms underlying its resistance to excess Mn(Ⅱ), we conducted a comparative proteome analysis. The data revealed that both 10 mM and 50 mM Mn(Ⅱ) significantly up-regulated the expression of proteins involved in Mn(Ⅱ) transport (MntE), oxidative stress response (alkyl hydroperoxide reductase and the Suf system), and amino acid metabolism (arginine, cysteine, methionine, and phenylalanine). We further investigated the role of cysteine metabolism in Mn(Ⅱ) resistance by examining the function of its downstream product, H 2 S. Consistent with the up-regulation of cysteine desulfurase, we detected an elevated level of H 2 S in Halomonas sp. MNB13 cells under Mn(Ⅱ) stress, along with increased intracellular levels of H 2 O 2 and O 2 •−. Upon exogenous addition of H 2 S, we observed a significant restoration of the growth of Halomonas sp. MNB13. Moreover, we identified decreased intracellular levels of H 2 O 2 and O 2 •− in MNB13 cells, which coincided with a decreased formation of Mn-oxides during cultivation. In contrast, in cultures containing NaHS, the residual Mn(Ⅱ) levels were higher than in cultures without NaHS. Therefore, H 2 S improves Mn(Ⅱ) tolerance by eliminating intracellular reactive oxygen species rather than decreasing Mn(Ⅱ) concentration in solution. Our findings indicate that cysteine metabolism, particularly the intermediate H 2 S, plays a pivotal role in Mn(Ⅱ) resistance by mitigating the damage caused by reactive oxygen species. These findings provide new insights into the amino acid mechanisms associated with Mn(Ⅱ) resistance in bacteria. • High levels of Mn(Ⅱ) affected the metabolism of certain amino acids in MNB13. • Cysteine desulfurase responsible for H 2 S production was significantly up-regulated. • Increased amounts of H 2 S, H 2 O 2 , and O 2.•− were detected in MNB13 under Mn(Ⅱ) stress • H 2 S restored the growth of MNB13 and reduced H 2 O 2 and O 2.•− intracellular levels • H 2 S decreased the formation of Mn(Ⅱ)-oxides by MNB13. [ABSTRACT FROM AUTHOR]

Volatile organic compounds (VOCs) undergo substantial photochemical losses during their transport from emission sources to receptor sites, resulting in serious implications for their source apportionment and ozone (O 3) formation. Based on the continuous measurements of VOCs in suburban Jinan in August 2022, the effects of photochemical losses on VOC source contributions and O 3 formation were evaluated in this study. The observed and initial concentrations of total VOCs (TVOC) were 12.0 ± 5.1 and 16.0 ± 7.4 ppbv, respectively. Throughout the observation period, alkenes had the most prominent photochemical losses (58.2%), followed by aromatic hydrocarbons (23.1%), accounting for 80.6% and 6.9% of the total losses, respectively. During high O 3 episodes, the photochemical loss of VOCs was 6.9 times higher than that during the cleaning period. Alkene losses (exceeding 67.3%), specifically losses of isoprene, propylene, ethylene, and n-butene, dominated the total losses of VOCs during the O 3 increase period. Eight sources of VOCs were identified by positive matrix factorization (PMF) based on the observed and initial concentration data (OC-PMF and IC-PMF, respectively). Concentrations of all emission sources in the OC-PMF were underestimated by 2.4%–57.1%. Moreover, the contribution of each emission source was over- or underestimated compared with that in case of the IC-PMF. The contributions of biogenic and motor vehicle exhaust emissions were underestimated by 5.3 and 2.8 percentage points, respectively, which was associated with substantial oxidation of the emitted high-reactive species. The contributions of coal/biomass burning and natural gas were overestimated by 2.4 and 3.9 percentage points, respectively, which were related to the emission of low-reactive species (acetylene, ethane, and propane). Based on our results, the photochemical losses of VOCs grossly affect their source apportionment and O 3 formation. Thus, photochemical losses of VOCs must be thoroughly accounted to establish a precise scientific foundation for air-pollution control strategies. [Display omitted] • Photochemical losses contributed 58.2% of initial alkenes and 23.1% of initial aromatics. • Photochemical loss of VOCs during high ozone period was 6.9 times higher than that during cleaning period. • Source apportionment based on measured VOCs might lead to biased conclusions. [ABSTRACT FROM AUTHOR]

Hierarchical MnO 2 -based fiber-in-tube and particle-in-tube nanostructures with unique chemical compositions and gradient pores have been rationally designed and constructed using a simple self-template method for high-performance asymmetric supercapacitors, which deliver extraordinarily excellent energy density. • MnO 2 -based fiber-in-tube and particle-in-tube nanostructures are designed. • The hierarchical architectures are fabricated via a simple self-template approach. • The novel nanotubes yield superior specific capacity as supercapacitor electrodes. • The asymmetric supercapacitors deliver extraordinarily excellent energy density. Manganese dioxide (MnO 2) promises for high-performance asymmetric suprecapacitors, owing to its high theoretical capacity, abundant source, and low cost. However, insufficient practically-achievable capacity and relatively narrow voltage window in alkaline electrolyte are blocking high energy density of MnO 2 -based supercapacitors, where strategies for activating its capacitive performance and widening voltage window are the top priorities to solve the bottleneck problems. Herein, both the fiber-in-tube (NCCM-FiT) and particle-in-tube (NCCM-PiT) nanostructures coulping active NiCoO x nanoparticles and conductive carbon with MnO 2 tubes have been purposely fabricated, using the electrospun nickel cobalt oxides/carbon nanofibers (NCO/CNFs) as the self-template agents for enhanced energy density of MnO 2 -based supercapacitors. These hierarchical hollow nanotubes with gradient pores and unique compositions yield excellent capacitive properties, in terms of a competitive capacity (431.7 F g−1 or 431.7 C g−1, 0.5 A g−1), which is 2.7 times that of the MnO 2 nanotubes-based electrodes. A maximum energy density of 46.4 Wh kg−1 is obtained at the power density of 400 W kg−1 for the asymmetric device assembled with the NCCM-PiT-based positive electrode and the electrospun CNFs-based negative electrode. The remarkable energy density demonstrated by these hierarchical hollow nanotubes exemplifies a novel and effective design in electrode materials for the asymmetric supercapacitors (ASCs) with superior performance. [ABSTRACT FROM AUTHOR]

Acute pancreatitis (AP) is one of the most common digestive tract diseases, but effective drug therapy is still lack. Regenerating gene protein 3α (Reg3α) administration significantly reduced the severity of AP in mice. HTD4010 is a new 15 amino acid long synthetic peptide and its biological activities are similar to Reg3α. This study aimed to explore whether HTD4010 could protect pancreatic acinar cells against necrosis and decrease the inflammatory response in AP, and thus to explore underlying mechanisms. It was shown that administration of HTD4010 alleviated significantly the severity of biliary AP (BAP), characterized as less degree of pancreatic histological damage and acinar cell injury (both apoptosis and necroptosis), lower levels of serum amylase and pro-inflammatory cytokines. Moreover, HTD4010 down-regulated the expression of toll-like receptor 4 (TLR4) protein, and TLR4 deficiency eliminated the protective effect of HTD4010 on BAP in mice. In conclusion, these results showed that HTD4010 could alleviate the severity of pancreatitis, reduce the acinar cells necrosis and inflammatory response possibly by TLR4 signaling pathway in AP. • HTD4010 is a new 15 amino acid long synthetic peptide. • HTD4010 reduced the inflammatory response and acinar cells necrosis in BAP mice. • HTD4010 could alleviate the severity of AP by TLR4 signaling pathway. [ABSTRACT FROM AUTHOR]

The study proposes a method for measuring the electrical properties of orange juice by using two magnetic fluxes with the same frequency (or synchronous magnetic fluxes) at 400–700 Hz. The juice was passed through two spiral glass tubes, which formed the secondary coils of the transformer with different connection modes. Five measurement points ( a , b , o , − a , and − b ) were arranged at different terminals of the two coils to evaluate output voltages ( U − aa , U oa , U − bb , and U ob ) under the fluxes. Control parameters included the excitation voltage ( U P ), frequency, and phase difference. Results indicated that the output voltage of the juice increased linearly with increasing excitation voltage at all points. In-phase output voltages were higher than reverse-phase output voltages. The value for λ − aa ( U − aa / U P ) remained stable as the excitation voltage increased. In addition, different physicochemical properties of orange juice caused a change in the output voltages, which was consistent with Ohm's law . Soluble solids content and U − bb were linearly correlated, showing R 2 values at 0.875 and a root-mean-square error of 0.702 Brix° at 20 V and 700 Hz. The method showed potential for the rapid determination of the quality of liquid foods by using magnetic flux-induced electrical parameters. [ABSTRACT FROM AUTHOR]

Trichinellosis is caused by Trichinella spiralis , a meat-borne zoonotic disease transmitted to humans through the consumption of infected undercooked or raw meat. Surveillance using safe and precise diagnostic tools to diagnose T. spiralis in sheep is needed to assess the incidence and probability of transmission from sheep to humans. In this study, we developed a real-time PCR assay to detect T. spiralis DNA in ovine muscle samples that can be used as an alternative surveillance tool to ensure food safety using newly designed primers. The assay is specific for the Scfld4 gene of Trichinella (T1) and enables the detection of larvae in ovine muscle tissue samples with high sensitivity and specificity. Trichuris ovis, Oesophagostomum dentatum, Haemonchus contortus, and Bunostomum trigonocephalum showed no nonspecific amplification. The assay could detect Trichinella DNA concentrations as low as 0.0026 ng/μL, equivalent to 0.0064 larvae, indicating a high sensitivity for T. spiralis detection. We used this real-time PCR to detect 73 ovine muscle samples from an ovine abattoir, and five samples tested positive via real-time PCR but negative via microscopy. This assay may provide a more specific and sensitive method for rapidly detecting Trichinella larvae in ovine muscle tissues. [Display omitted] • Trichinella larvae can be detected quickly in ovine muscle tissues using qPCR. • The qPCR is an alternative food safety surveillance tool. • The qPCR is more sensitive and specific to other methods of detection. • No specific amplification signal for other parasites, including T. ovis, O. dentatum, H. contortus, and B. trigonocephalum. [ABSTRACT FROM AUTHOR]

The combined effect of a gelatin-derived cryoprotective peptide (Pro–Ala–Gly–Tyr) and a non-peptide antioxidant (caffeic acid) in a fish mince model system was investigated. The physicochemical properties (peroxide value, thiobarbituric acid-reactive substances, total sulfhydryl group content and protein carbonyl content), water distribution and thermal properties of Japanese sea bass mince were determined before and after 6 freeze-thaw cycles. Mince supplemented with a combination of a cryoprotective tetrapeptide (12.5 ppm) and 50 ppm caffeic acid had the lowest peroxide value and comparable thiobarbituric acid-reactive substances to 100 ppm caffeic acid. The cryoprotective tetrapeptide at 12.5 ppm also showed the greatest effect when combined with caffeic acid in lowering protein oxidation, in protecting the total sulfhydryl group content and in having the lowest protein carbonylation, which led to least myosin heavy chain denaturation. The state of water was studied using low-field nuclear magnetic resonance. The tetrapeptide reduced the formation of free water in the mince, especially in conjunction with caffeic acid. Thus, the cryoprotective tetrapeptide worked with caffeic acid in retarding the quality losses in the mince induced by freezing and thawing. [ABSTRACT FROM AUTHOR]

Nowadays, new snacks using alternative raw materials and ingredients are the critical driving forces for the snack food industry development. Wheat is an abundant cereal resource with the potential for chips making. In the present study, protease-treated wheat flour improved the quality of wheat chips by weakening the gluten strength of dough and reducing the chips' hardness from 6019 ± 306 N to 785 ± 200 N. The optimal enzymatic hydrolysis parameters were established as 1.44 × 104 U bromelain/100 g flour at 50 °C for 60 min. Bromelain significantly reduced the polymeric proteins content (from 15.81 ± 0.52% to 0.82 ± 0.03%) and glutenin macropolymer (GMP, from 14.95 ± 0.23% to 2.06 ± 0.05%), swelling power, gelatinization enthalpy (from 8.82 ± 0.39 to 7.31 ± 0.27 J/g), and viscosities, but increased the solubility (from 3.9 ± 0.1% to 12.8 ± 0.7%) of wheat flour. Moreover, protease hydrolysis significantly decreased the elastic modulus and viscous modulus (P < 0.05); values and the gluten network in the protease-treated dough showed different degradation degrees. The reconstituted wheat chips proved that GMP was the main factor affecting the chip hardness. The present study suggests that protease hydrolysis, especially bromelain, has great potential as a flour modification technology for producing baked wheat chips. • Wheat flour treated with protease improved the quality of wheat chips. • The optimal enzymatic hydrolysis parameters were: 1.44 × 104 U/100 g flour at 50 °C for 60 min. • Protease hydrolysis weakened the gluten strength and changed the starch properties. • Bromelain showed stronger hydrolytic effect on wheat proteins and rheological properties. • Glutenin macropolymer played a key role in the hardness of wheat chips. [ABSTRACT FROM AUTHOR]

Abstract: In this study, a glucose oxidase (GOX), papain and xylanase combination was developed for fresh whole wheat dough for both browning inhibition and rheological improvement. Measurements of carotenoids extracted from enzyme-treated doughs showed that 0.001% (w/w) GOX could catalyze the oxidization of 40.0% carotenoids and thus cause a decrease of browning index (BI) by 5.20 during dough preparation. For 24 h browning inhibition, 0.010% (w/w) xylanase and papain individuals were able to separately act on the phenolic compounds and polyphenol oxidase, leading to lower BI rises (5.36 and 7.04, respectively) of doughs as compared to the BI rise (13.53) of the control dough; however, 0.020% GOX caused a higher BI rise (16.60) than control although it made BI decrease by 6.34 at 0 h. Rheological investigations on enzyme-treated doughs revealed that both xylanase and papain led to decreases of elastic (G′) and viscous modulus (G″) of doughs while GOX caused increases of G′ and G″. Therefore, an optimal combination composed of 0.010% (w/w) xylanase, 0.005% (w/w) papain and 0.002% (w/w) GOX was carried out using orthogonal experimental design by comparing BI rises in 24 h, which was also proved as a rheological improver for fresh whole wheat dough. [Copyright &y& Elsevier]

The magnetic field is an emerging physical method used for food preservation, characterized by its high penetrability in food materials. The effect of a static magnetic field (MF, 2 mT) on frozen dough upon freeze-thaw treatments (3, 7, and 10 cycles) was investigated, with conventional freeze-thaw (CF) treatments being used as control. Results showed that the magnetic field ensured the baked bread made from dough a 17.59% larger specific volume and a 30.87% smaller crumb hardness. It accelerated the time for the dough to pass through the maximum ice crystal formation zone by 7 min and reduced the ice melting enthalpy from 63.55 J/g to 56.67 J/g after 10 freeze-thaw cycles. Nuclear magnetic resonance analysis suggested that the conversion of bound water to immobilized water and free water was inhibited. The yeast viability and activity were also better maintained, precisely as the observed increase of 18.18% and 42.23% in its survival and gassing power. After 10 freeze-thaw cycles, the free disulfide (SH) content was determined to be 1.642 μmol/g for CF doughs and 1.624 μmol/g for MF ones, respectively. A reduction of 24.26% in glutenin macropolymer depolymerization was achieved, indicated a more stable gluten structure under the magnetic field. [Display omitted] • Magnetic field improved the quality of frozen dough bread. • Lower depolymerization degree was detected in gluten using magnetic field. • Yeast survival and gassing power were better preserved under magnetic field. • Magnetic field inhibited water migrations and status change. [ABSTRACT FROM AUTHOR]

This study aimed to efficiently and selectively hydrolyze maize starch via induced electric field (IEF). The role of electrical conductivity in regulating hydrolysis efficiency and selectivity was investigated, and the structural and physicochemical properties of hydrolyzed products were characterized. Compared with water-bath heating, IEF induced the rapid and ordered migration of charged species, thus increasing the hydrolysis rate and narrowing the molecular weight distribution of hydrolyzed starch. The addition of KCl enhanced IEF-assisted hydrolysis due to the absorption of additional electrical energy into the reaction system. After hydrolysis, the molecular chains of maize starch exhibited cleavage, the granular surface became rough, the crystallinity increased, the gelatinization enthalpy slightly increased, the viscosity of starch paste reduced, the hardness of starch gel declined, and the re-crystallization showed no significant improvement. Overall, this study extends the potential applications of electro-techniques in preparing hydrolyzed starch with narrow molecular weight distribution in food and material fields. [Display omitted] • Induced electric field (IEF) improved the hydrolysis efficiency of maize starch. • IEF increased the hydrolysis selectivity of maize starch. • The efficiency and selectivity enhanced with the increasing conductivity. • IEF-assisted hydrolysis roughened granular surface and increased crystallinity. • IEF-assisted hydrolysis decreased viscosity but showed no effect on re-crystallization. [ABSTRACT FROM AUTHOR]