1. Atranones from Stachybotrys chartarum and their antitumor activities in MG-63 human osteosarcoma cells.
- Author
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Qin, Ya-Dong, Fang, Feng-Man, Zhou, Juan-Juan, Wang, Rong-Bin, Xu, Sheng-Xiang, Li, Lin-hua, and Zhang, Han-Lin
- Subjects
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ANTINEOPLASTIC agents , *APOPTOSIS , *CELL lines , *CELL physiology , *FLOW cytometry , *FUNGI , *MASS spectrometry , *OSTEOSARCOMA , *RESEARCH funding , *SPECTRUM analysis , *DESCRIPTIVE statistics , *PHARMACODYNAMICS - Abstract
Two new atranones T and U (1 and 2), and three known analogues atranone B (3), atranone Q (4), and stachatranone C (5) were isolated from the toxigenic fungus Stachybotrys chartarum. Their structures and absolute configurations were elucidated by spectroscopic data and calculated ECD analyses. The cytotoxicities of all the atranones (1 – 5) were evaluated against MG-63 human osteosarcoma cell lines. Compound 4 exhibited significant cytotoxic effect against MG-63 with IC 50 value of 8.6 μM, being more active than the positive control, 5-FU (IC 50 10.4 μM). Morphological features of apoptosis activities were evaluated in 4 -treated MG-63 cells. Compound 4 effectively induced apoptosis of MG-63, which was associated with G 0 /G 1 -phase cell cycle arrest. Flow cytometric analysis showed that the treatment by 4 significantly induced MG-63 cell apoptosis in a dose-dependent manner. Unlabelled Image [ABSTRACT FROM AUTHOR]
- Published
- 2020
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