53 results on '"Wang Rong"'
Search Results
2. Correlation between Insulin Resistance and Microalbuminuria Creatinine Ratio in Postmenopausal Women.
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Na, Han, Wang, Rong, Zheng, Hai-Long, Chen, Xiao-Pan, and Zheng, Lin-Yang
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POSTMENOPAUSE , *INSULIN resistance , *DIASTOLIC blood pressure , *GLYCOSYLATED hemoglobin , *SYSTOLIC blood pressure - Abstract
Objective. To study the relationship between insulin resistance and urinary microalbumin creatinine ratio in postmenopausal women. Methods. The selected research group comprised 104 postmenopausal women with type 2 diabetes who were admitted to the Department of Endocrinology in the green card center at the First Affiliated Hospital of Hainan Medical University between 2017 and 2019 inclusive. Ninety-eight postmenopausal women with the normal blood glucose metabolism hospitalized in the same period were used as the control group. The age, body mass index, systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate, fasting blood glucose, fasting insulin (FINS), glycosylated hemoglobin (HbA1c), total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C), and urinary albumin-creatinine ratio (UACR) were analyzed. The insulin resistance index (HOMR-IR) was calculated, and the correlation between IR and UACR was analyzed. Results. Levels of HOMA-IR, SBP, HbA1c, HDL-C, LDL-C, TC, TG, FPG, FINS, and UACR in the study group were higher than those in the control group, and a significant difference was found between the groups (P < 0.05). The level of DBP in the study group was lower than that in the control group, and the difference was statistically significant (P < 0.05). Pearson correlation analysis showed that UACR was positively correlated with HOMA-IR and HbA1c (r = 0.254, r = 0.565, P < 0.01). Multiple linear stepwise regression analysis further showed that HOMA-IR and age were positively correlated with UACR (P < 0.05). Conclusion. There is a correlation between IR and UACR in postmenopausal women. IR is an independent risk factor for UACR. [ABSTRACT FROM AUTHOR]
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- 2022
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3. miR-29b Regulates Lung Cancer Progression by Downregulating FEM1B and Inhibiting the FOX01/AKT Pathway.
- Author
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Zhang, Huanrong, Wang, Rong, and Deng, Qiuhua
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LUNG cancer , *VASCULAR endothelial growth factors , *NON-small-cell lung carcinoma , *CANCER invasiveness , *SMALL molecules , *CANCER cells - Abstract
Purpose. Lung cancer is a relatively common type of cancer, and the incidence rate has been on the rise in recent years. MicroRNAs are a class of endogenous small RNA molecules, which are essential for the posttranscriptional regulation of genes. miR-29b is closely related to the occurrence and development of tumors, including prostate cancer, colon cancer, and breast cancer. However, few studies have been performed to explore the expression and pathway of miR-29b in non-small-cell lung cancer (NSCLC). Methods. Using bioinformatics analysis, we found that patients with low relative expression of the miR-29b gene have a low long-term survival rate. The results of in vitro research showed that when miR-29b expression was upregulated, the invasion, migration, and proliferation of A549 and NCI-H-1792 cells was inhibited, and the apoptosis was accelerated. Results. The results showed that FEM1B is a miR-29b target gene, and the expressions of FEM1B and miR-29b were negatively correlated. Like the upregulation of miR-29b expression, silencing the FEM1B expression could also impair the invasion, migration, and proliferation abilities of A549 and NCI-H-1792 cells. When FEM1B expression was restored, the inhibitory effect of miR-29b could be reversed. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot (WB) analysis showed that overexpression of miR-29b could inhibit the expression of FEM1B, AKT, vascular endothelial growth factor (VEGF), and Sirt3 in A549 and NCI-H-1792 cells and upregulate the expression of FOXO1 protein. Conclusion. The results of this study indicate that miR-29b inhibits the proliferation and deterioration of NSCLC cells by targeting FEM1B and inhibiting the activation of the FOXO1/AKT pathway. miR-29b may become a new target for the clinical diagnosis and treatment of lung cancer, and it is expected to become a new inhibitor of NSCLC. [ABSTRACT FROM AUTHOR]
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- 2022
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4. Pantograph Catenary Performance Detection of Energy High-Speed Train Based on Machine Vision.
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Wang, Rong, Li, Wan, Tan, Lizhi, Liu, Haiyu, Le, Qiqing, Jiang, Songyun, and Nguyen, Kevin T.
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COMPUTER vision , *HIGH speed trains , *CATENARY , *PANTOGRAPH , *SYSTEM failures , *LOW vision - Abstract
With the rapid development of high-speed rail in China, addressing the issue of safety assurance during the operation of the train is very important. A very important part of a train's power supply system is the pantograph and catenary system, which consists of a pantograph and a catenary. Failure of the pantograph-catenary system can cause significant damage to the normal operation of the train. The dynamic performance of the pantograph-catheter system must be detected in real time during the operation of the train. This paper is based on the study and analysis of pantograph-catheter dynamic performance parameters and developed a system for real-time detection of pantograph-catheter dynamic performance parameters based on a car visual system. The results are as follows: based on this detection method, the visual error is low and the accuracy is high. The machine-based directional height detection module developed in this paper has a good detection effect and high test accuracy; the arcing detection module designed in this paper can effectively detect the arcing and store the arcing pictures and can display the duration of single arcing and the arcing rate of the section in real-time. The practical application effect is good. The results show that the focal length of the camera lens is 16 mm, and the error of the machine vision system is low. The system designed in this paper may make a great contribution to the operation condition monitoring and fault diagnosis of the pantograph-catenary system of a high-speed train in the future. [ABSTRACT FROM AUTHOR]
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- 2022
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5. Effectiveness of Chinese Native Culture Education for Improving Undergraduate Nursing Students' Transcultural Self-Efficacy.
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Wang, Rong, Duan, GongXiang, Wu, YuanYuan, Su, YinHua, Li, JianZhi, Liao, Li, and Xu, Huilan
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NURSING students , *UNDERGRADUATES , *SELF-efficacy , *CULTURAL competence ,UNDERGRADUATE education - Abstract
Aim. The aim of this study is to evaluate the impact of Chinese native culture education based on Chinese native culture on the intercultural competence of undergraduate nursing students. Method. A quasi-experimental design with pretest and posttest was used. We recruited nursing students from 4 classes of the School of Nursing in our hospital in 2016 as research subjects. Undergraduate nursing students (n = 79) who completed one semester of education in indigenous Chinese culture completed a demographic questionnaire and the transcultural self-efficacy tool (TSET). Chinese native culture education is the topics related to nursing, consistent with the culture of Chinese patients under the background of Chinese native culture, including the dietary habits, taboos, religions, values, particularly Chinese medicine, and specific diseases. The control group (n = 91) was students who did not participate in Chinese native culture education and completed the instrument during the same time frame. Result. Students who participated in Chinese native cultural education significantly improved their transcultural self-efficacy in three dimensions: cognition, emotion, and practice. Compared with the control group, the students in the cultural education group had higher change scores in three sizes of transcultural self-efficacy. Conclusion. When strengthening cultural education for undergraduate nursing students, adding content related to Chinese native culture can improve their transcultural self-efficacy and meet the growing cultural needs of patients. [ABSTRACT FROM AUTHOR]
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- 2022
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6. Liquid-Phase Synthesis of Nano-Zinc Oxide and Its Photocatalytic Property.
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Wang, Rong
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PRECIPITATION (Chemistry) , *ZINC oxide , *OXIDES , *SURFACE area , *PROBLEM solving , *PERFORMANCE theory - Abstract
To solve the problem of liquid-phase synthesis of nano-ZnO, a photocatalytic performance study was proposed. In this study, the microwave homogeneous precipitation method was used to add different types and amounts of surfactants. The synthesis of nano-zinc oxide was controlled by changing the reaction system conditions. The photocatalytic properties of nano-zinc oxide for degrading three water-soluble dyes were preliminarily studied and discussed. The results show that the photocatalytic performance of nano-ZnO is closely related to its size, morphology, specific surface area, and even crystallographic direction. [ABSTRACT FROM AUTHOR]
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- 2022
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7. Development of Attenuated Total Reflectance Mid-Infrared (ATR-MIR) and Near-Infrared (NIR) Spectroscopy for the Determination of Resistant Starch Content in Wheat Grains.
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Wang, Rong, Wei, Xia, Wang, Hongpan, Zhao, Linshu, Zeng, Cengli, Wang, Bingrui, Zhang, Wenying, Liu, Luxiang, and Xu, Yanhao
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STARCH content of grain , *ATTENUATED total reflectance , *WHEAT , *STANDARD deviations , *WHEAT breeding , *SPECTROMETRY , *MID-infrared spectroscopy , *NEAR infrared spectroscopy - Abstract
The chemical method for the determination of the resistant starch (RS) content in grains is time-consuming and labor intensive. Near-infrared (NIR) and attenuated total reflectance mid-infrared (ATR-MIR) spectroscopy are rapid and nondestructive analytical techniques for determining grain quality. This study was the first report to establish and compare these two spectroscopic techniques for determining the RS content in wheat grains. Calibration models with four preprocessing techniques based on the partial least squares (PLS) algorithm were built. In the NIR technique, the mean normalization + Savitzky–Golay smoothing (MN + SGS) preprocessing technique had a higher coefficient of determination (R c 2 = 0.672; R p 2 = 0.552) and a relative lower root mean square error value (RMSEC = 0.385; RMSEP = 0.459). In the ATR-MIR technique, the baseline preprocessing method exhibited a better performance regarding to the values of coefficient of determination (R c 2 = 0.927; R p 2 = 0.828) and mean square error value (RMSEC = 0.153; RMSEP = 0.284). The validation of the developed best NIR and ATR-MIR calibration models showed that the ATR-MIR best calibration model has a better RS prediction ability than the NIR best calibration model. Two high grain RS content wheat mutants were screened out by the ATR-MIR best calibration model from the wheat mutant library. There was no significant difference between the predicted values and chemical measured values in the two high RS content mutants. It proved that the ATR-MIR model can be a perfect substitute in RS measuring. All the results indicated that the ATR-MIR spectroscopy with improved screening efficiency can be used as a fast, rapid, and nondestructive method in high grain RS content wheat breeding. [ABSTRACT FROM AUTHOR]
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- 2021
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8. Coupling Coordination and Prediction Research of Tourism Industry Development and Ecological Environment in China.
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Yang, Yun, Wang, Rong, and Tan, Junlan
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TOURISM , *ECOTOURISM , *TOURISM research , *COUPLES - Abstract
Based on the data relevant to China's tourism industry and ecological environment from 2005 to 2018, the entropy method and the coupling coordination degree model were used to analyze the coupling degree and coupling coordination degree of the tourism industry's development and the ecological environment, and the GM (1.1) gray model was used to predict these values in the future. The model provided predictions and illustrated the trend in the changes in the degree of coordination between the development of China's tourism industry and the ecological environment for the next five years. The results show the following. (1) The coupling degree between China's tourism industry and the ecological environment was between 0.3 and 0.5. It was in the low-level coupling stage in 2005 and 2006 and generally low in 2005–2009, lower than the average level of 0.4204. In 2018, the coupling degree between the development of China's tourism industry and the ecological environment was mainly in a stage of antagonism, reaching a peak of 0.4998 in 2013. The coupling degree declined slightly from 2013 to 2015 and then began to show an upward trend again in 2016. (2) The average coupling coordination level of China's tourism industry development and ecological environment was 0.4266, which is in the medium coupling coordination stage. From 2007 to 2013, it was mainly in the phase of moderate coupling and coordination and high coupling and coordination from 2014 to 2018. (3) From the prediction results, the coupling degree will enter the coupling degree running-in stage in 2021 and the extreme coordination stage in 2023, but the coupling growth level during the running-in period will be significantly lower than the coupling coordination degree. Coordinated development of the two systems will take a long time, and active economic policies need to be adopted to promote their coordinated development. [ABSTRACT FROM AUTHOR]
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- 2021
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9. A Novel Inertial-Aided Star Pattern Matching Method for Distributed Multiple FoVs.
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Wang, Rong, Cao, Yuxuan, Liu, Jianye, and Xiong, Zhi
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PATTERN matching , *HIGH-speed aeronautics , *INFORMATION measurement - Abstract
An aerospace vehicle in high-speed flight involves distributed multiple fields of view (FoVs) of a star sensor, and the maneuvering of the vehicle requires inertial-aided star pattern matching. Accordingly, an inertial-aided star pattern matching method for distributed multiple FoVs is proposed. First, the observation and fusion principles of distributed multiple FoVs are illustrated. Then, an inertial-aided star pattern matching scheme is designed based on the space-time distribution of the line-of sight (LoS) vector and inertial measurement information. Next, an LoS vector estimation method assisted by inertial information and the dynamic establishment of a distributed local catalog are proposed. Finally, in a simulation, the dynamic establishment of the part catalog improves the efficiency of star pattern matching by reducing the catalog scale, and the usability of inertial-aided star pattern matching is analyzed for different gyro and accelerometer precisions. [ABSTRACT FROM AUTHOR]
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- 2020
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10. A Novel Inertial-Aided Star Pattern Matching Method for Distributed Multiple FoVs.
- Author
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Wang, Rong, Cao, Yuxuan, Liu, Jianye, and Xiong, Zhi
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PATTERN matching , *HIGH-speed aeronautics , *INFORMATION measurement - Abstract
An aerospace vehicle in high-speed flight involves distributed multiple fields of view (FoVs) of a star sensor, and the maneuvering of the vehicle requires inertial-aided star pattern matching. Accordingly, an inertial-aided star pattern matching method for distributed multiple FoVs is proposed. First, the observation and fusion principles of distributed multiple FoVs are illustrated. Then, an inertial-aided star pattern matching scheme is designed based on the space-time distribution of the line-of sight (LoS) vector and inertial measurement information. Next, an LoS vector estimation method assisted by inertial information and the dynamic establishment of a distributed local catalog are proposed. Finally, in a simulation, the dynamic establishment of the part catalog improves the efficiency of star pattern matching by reducing the catalog scale, and the usability of inertial-aided star pattern matching is analyzed for different gyro and accelerometer precisions. [ABSTRACT FROM AUTHOR]
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- 2020
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11. Research on China's Ecological Welfare Performance Evaluation and Improvement Path from the Perspective of High-Quality Development.
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Wang, Rong and Feng, Yue
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PERFORMANCE evaluation , *DATABASES - Abstract
Based on the data of China's ecological environment from 2006 to 2018, the paper uses the super-efficiency DEA and Malmquist index methods to evaluate China's ecological welfare performance from a static and dynamic perspective. Based on this, the Theil index is used to analyze the group's ecological welfare performance. The internal and intergroup differences show that, from the static evaluation results, China's ecological welfare performance is in a situation of "high in the east, low in the west, and average in the central region." There is not much difference between the eastern and central regions, while the ecological welfare performance in the western region is low. From the results of dynamic evaluation, the overall level of regional ecological welfare performance in China has improved in recent years, and the average Malmquist index has exceeded 1, indicating that the growth pattern of ecological welfare performance has shifted to high quality, but the degree of increase in each region is different. There is still much room for improvement in ecological welfare performance; from the perspective of intragroup and intergroup differences, the intragroup differences and intergroup differences in the three major regions have generally maintained a continuous downward trend, and the contribution of the differences in ecowelfare performance between group rate has a clear advantage. Finally, corresponding suggestions are put forward based on the empirical results of the paper. [ABSTRACT FROM AUTHOR]
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- 2020
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12. Passivity-Based Control for Rocket Launcher Position Servo System Based on ADRC Optimized by IPSO-BP Algorithm.
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Wang, Rong-lin, Lu, Bao-chun, Hou, Yuan-long, and Gao, Qiang
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SERVOMECHANISMS , *ARTIFICIAL intelligence , *AUTOMATION , *INTELLIGENT agents , *HAMILTONIAN systems - Abstract
In order to achieve better motion accuracy and higher robustness of the shipborne rocket launcher position servo system driven by a permanent magnet synchronous motor (PMSM), a passivity-based controller based on active disturbance rejection control (ADRC) optimized by improved particle swarm optimization-back propagation (IPSO-BP) algorithm is proposed in this paper. The convenient method of interconnection and damping assignment and passivity-based control (IDA-PBC) is adopted to establish the port controlled Hamiltonian system with dissipation (PCHD) model of PMSM. To further enhance the robustness and adaptability of traditional ADRC, an BP algorithm is introduced to on-line update the proportional, integral, and derivative gains of ADRC. Furthermore, to improve the learning capability, the improved PSO algorithm is adopted to optimize the learning rates of the back propagation neural networks. The results of numerical simulation and prototype test indicate that the proposed IPSO-BP-ADRC-PBC controller has better static and dynamic performance than the ADRC-PBC and BP-ADRC-PBC controller with fixed learning rate. [ABSTRACT FROM AUTHOR]
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- 2018
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13. Effects of Zusanli and Ashi Acupoint Electroacupuncture on Repair of Skeletal Muscle and Neuromuscular Junction in a Rabbit Gastrocnemius Contusion Model.
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Yu, Zhan-ge, Wang, Rong-guo, Xiao, Cheng, Zhao, Jun-yun, Shen, Qian, Liu, Shou-yao, Xu, Qian-wei, Zhang, Qing-xi, and Wang, Yun-ting
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Objective. To explore the effects of electroacupuncture (EA) at ST36 (EA-ST36) and at Ashi acupoints (EA-Ashi) on skeletal muscle repair. Methods. Seventy-five rabbits were randomly divided into five groups: normal, contusion, EA-Ashi, EA-ST36, and EA at Ashi acupoints and ST36 (EA-AS). EA (0.4 mA, 2 Hz, 15 min) was applied after an acute gastrocnemius contusion. The morphology of myofibers and neuromuscular junctions (NMJs) and expressions of growth differentiation factor-8 (GDF-8), acetylcholinesterase (AChE), Neuregulin 1 (NGR1), and muscle-specific kinase (MuSK) were assessed 7, 14, and 28 days after contusion. Results. Compared with that in contusion group, there was an increase in the following respective parameters in treatment groups: the number and diameter of myofibers, the mean staining area, and continuities of NMJs. A comparison of EA-Ashi and EA-ST36 groups indicated that average myofiber diameter, mean staining area of NMJs, and expressions of AChE and NRG1 were higher in EA-Ashi group, whereas expression of GDF-8 decreased on day 7. However, increases in myofiber numbers, expressions of MuSK and AChE, as well as decreases in GDF-8 expression, and the discontinuities were observed in EA-ST36 group on the 28th day. Conclusion. Both EA-ST36 and EA-Ashi promoted myofiber regeneration and restoration of NMJs. EA-Ashi was more effective at earlier stages, whereas EA-ST36 played a more important role at later stages. [ABSTRACT FROM AUTHOR]
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- 2016
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14. Effects of Caloric Intake on Learning and Memory Function in Juvenile C57BL/6J Mice.
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Xu, Bao-Lei, Wang, Rong, Ma, Li-Na, Dong, Wen, Zhao, Zhi-Wei, Zhang, Jing-Shuang, Wang, Yu-Lan, and Zhang, Xu
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DIET , *LEARNING in animals , *ANIMAL memory , *ADOLESCENCE , *MAZE tests , *DOWNREGULATION , *PEROXISOME proliferator-activated receptors , *SPATIAL memory - Abstract
Dietary composition may influence neuronal function as well as processes underlying synaptic plasticity. In this study, we aimed to determine the effect of high and low caloric diets on a mouse model of learning and memory and to explore mechanisms underlying this process. Mice were divided into three different dietary groups: normal control (n = 12), high-caloric (HC) diet (n = 12), and low-caloric (LC) diet (n = 12). After 6 months, mice were evaluated on the Morris water maze to assess spatial memory ability. We found that HC diet impaired learning and memory function relative to both control and LC diet. The levels of SIRT1 as well as its downstream effectors p53, p16, and peroxisome proliferator-activated receptor γ (PPARγ) were decreased in brain tissues obtained from HC mice. LC upregulated SIRT1 but downregulated p53, p16, and PPARγ. The expressions of PI3K and Akt were not altered after HC or LC diet treatment, but both LC and HC elevated the levels of phosphorylated-cAMP response element-binding protein (p-CREB) and IGF-1 in hippocampal CA1 region. Therefore, HC diet-induced dysfunction in learning and memory may be prevented by caloric restriction via regulation of the SIRT1-p53 or IGF-1 signaling pathways and phosphorylation of CREB. [ABSTRACT FROM AUTHOR]
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- 2015
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15. Integrated Navigation Method of Aerospace Vehicle Based on Rank Statistics.
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Kang, Jun, Xiong, Zhi, Wang, Rong, and Zhang, Xinrui
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RANKING (Statistics) , *DISTRIBUTION (Probability theory) , *HIGH-speed aeronautics , *GAUSSIAN distribution , *RELIABILITY in engineering , *NAVIGATION - Abstract
The large dynamic and high-speed flight of aerospace vehicle will bring unpredictable conditions to its navigation system, resulting in that its system random noise probability distribution will no longer meet the preconditions of Gaussian distribution preset by the existing filter algorithm, thus reducing the accuracy of the navigation system. So, it is very important to propose an effective method to solve the filter problem of the navigation system in non-Gaussian distribution to improve the accuracy of the navigation system. Therefore, an integrated navigation method of aerospace vehicle based on rank statistics (LRF) has been proposed in this paper. Firstly, based on the flight characteristics of aerospace vehicles, an accurate gravity calculation model has been established to improve the accuracy of system modelling. Then, the state equation and measurement equation of integrated navigation system have been established. In combination with the rank filter algorithm as well as the determined weights, sampling points are calculated and nonlinearly propagated through the transition matrix to achieve an accurate estimation about the predicted values of the state quantities and measurement quantities and the covariance matrix. In turn, it simulates the probability distribution of the system state effectively. Therefore, when the system random noise probability distribution of the aerospace vehicle does not meet the Gaussian distribution due to various interference factors in the actual flight process, the algorithm can simulate the probability distribution of the actual system to the greatest extent, to improve the accuracy of the integrated navigation system and enhance the reliability of the navigation system ultimately. [ABSTRACT FROM AUTHOR]
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- 2023
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16. Hsa-let-7d-5p Promotes Gastric Cancer Progression by Targeting PRDM5.
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Gao, Xiang, Liu, Huiqi, Wang, Rong, Huang, Mingyu, Wu, Qiong, Wang, Yang, Zhang, Wei, and Liu, Yongnian
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STOMACH cancer , *CANCER invasiveness , *DIGESTIVE organs , *PROTEIN domains , *CELL proliferation - Abstract
Gastric cancer (GC) is a common malignant tumor in the digestive system and a significant health burden worldwide. In this study, we found that hsa-let-7d-5p was upregulated in GC cells, promoted GC cell proliferation, migration, and invasion, and reduced apoptosis. Moreover, we found that the expression of PRDM5 (PR domain protein 5) was downregulated in GC cells and upregulated in GC cells treated with hsa-let-7d-5p inhibitor. Further investigation showed that hsa-let-7d-5p was the target of PRDM5, and the functions of hsa-let-7d-5p on GC progression were rescued by PRDM5 overexpression in GC cells. Collectively, our findings suggested that hsa-let-7d-5p promoted the development of GC by targeting PRDM5, indicating that hsa-let-7d-5p could be a promising therapeutic molecule for the treatment of gastric cancer. [ABSTRACT FROM AUTHOR]
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- 2022
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17. Backward Trajectory and Multifractal Analysis of Air Pollution in Zhengzhou Region of China.
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Wang, Qizhen, Zhao, Tong, Wang, Rong, and Zhang, Ling
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AIR pollution , *AIR pollution potential , *AIR analysis , *SUSTAINABLE urban development , *POLLUTION , *SUSTAINABLE development - Abstract
With the continuous promotion of industrialization and urbanization, China's environmental pollution is becoming increasingly serious, which has caused considerable damage to the natural balance. Air pollution seriously harms people's physical and mental health, the ecological environment, and the social sustainable development of society. In this study, the backward trajectory model and multifractal methods were adopted to analyze air pollution in Zhengzhou. The backward trajectory analysis showed that most clusters of air pollution were from southern Hebei, eastern Shandong, and mid-western Henan, which were then transported to Zhengzhou. For the PSCF and CWT analyses, we selected four representative cities to explore how close the air pollution of Zhengzhou is to other areas on the basis of air polluted concentration. The results of several multifractal methods indicated that multifractality existed in the AQI time series of Zhengzhou and cross-correlations between Zhengzhou and each of the four cities. The widths of multifractal spectra showed that the air pollution in Zhengzhou was closest to that in Jinan, followed by Shijiazhuang, Zibo, and Luoyang. The CDFA analysis showed that carbon monoxide (CO), nitrogen dioxide (NO2), and inhalable particulate matter (PM10) had important influences on air pollution in Zhengzhou. These findings offer a useful reference for air pollution sources and their potential contributions in Zhengzhou, which can support policy makers in environmental governance and in achieving sustainable urban development. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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18. Bioinformatic Analysis of Immune Significance of RYR2 Mutation in Breast Cancer.
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Xu, Zhiquan, Xiang, Ling, Wang, Rong, Xiong, Yongfu, Zhou, He, Gu, Haitao, Wang, Jijian, and Peng, Linglong
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BREAST cancer prognosis , *STATISTICS , *GENETIC mutation , *MACROPHAGES , *BIOINFORMATICS , *CELLULAR signal transduction , *IMMUNITY , *SURVIVAL analysis (Biometry) , *KAPLAN-Meier estimator , *GENE expression profiling , *T cells , *TUMOR markers , *ALGORITHMS - Abstract
Background. Currently, immunotherapy is widely used for breast cancer (BC) patients, and tumor mutation burden (TMB) is regarded as a valuable independent predictor of response to immunotherapy. However, specific gene mutations and their relationship with TMB and tumor-infiltrating immune cells in BC are not fully understood. Methods. Comprehensive bioinformatic analyses were performed using data from The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) datasets. Survival curves were analyzed via Kaplan-Meier analysis. Univariate and multivariate Cox regression analyses were used for prognosis analysis. Gene set enrichment analysis (GSEA) was performed to explore regulatory mechanisms and functions. The CIBERSORT algorithm was used to calculate the tumor-infiltrating immune cell fractions. Results. We analyzed somatic mutation data of BC from TCGA and ICGC datasets and found that 19 frequently mutated genes were reported in both cohorts, namely, SPTA1, TTN, MUC17, MAP3K1, CDH1, FAT3, SYNE1, FLG, HMCN1, RYR2 (ryanodine receptor 2), GATA3, MUC4, PIK3CA, KMT2C, TP53, PTEN, ZFHX4, MUC16, and USH2A. Among them, we observed that RYR2 mutation was significantly associated with higher TMB and better clinical prognosis. Moreover, GSEA revealed that RYR2 mutation-enriched signaling pathways were related to immune-associated pathways. Furthermore, based on the CIBERSORT algorithm, we found that RYR2 mutation enhanced the antitumor immune response by enriching CD8+ T cells, activated memory CD4+ T cells, and M1 macrophages. Conclusion. RYR2 is frequently mutated in BC, and its mutation is related to increased TMB and promotes antitumor immunity; thus, RYR2 may serve as a valuable biomarker to predict the immune response. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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19. Bioinformatic Analysis of Immune Significance of RYR2 Mutation in Breast Cancer.
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Xu, Zhiquan, Xiang, Ling, Wang, Rong, Xiong, Yongfu, Zhou, He, Gu, Haitao, Wang, Jijian, and Peng, Linglong
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BREAST cancer prognosis , *STATISTICS , *GENETIC mutation , *MULTIVARIATE analysis , *REGRESSION analysis , *CANCER patients , *BIOINFORMATICS , *CELLULAR signal transduction , *GENOMICS , *KAPLAN-Meier estimator , *GENE expression profiling , *CONSORTIA , *BREAST tumors - Abstract
Background. Currently, immunotherapy is widely used for breast cancer (BC) patients, and tumor mutation burden (TMB) is regarded as a valuable independent predictor of response to immunotherapy. However, specific gene mutations and their relationship with TMB and tumor-infiltrating immune cells in BC are not fully understood. Methods. Comprehensive bioinformatic analyses were performed using data from The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) datasets. Survival curves were analyzed via Kaplan-Meier analysis. Univariate and multivariate Cox regression analyses were used for prognosis analysis. Gene set enrichment analysis (GSEA) was performed to explore regulatory mechanisms and functions. The CIBERSORT algorithm was used to calculate the tumor-infiltrating immune cell fractions. Results. We analyzed somatic mutation data of BC from TCGA and ICGC datasets and found that 19 frequently mutated genes were reported in both cohorts, namely, SPTA1, TTN, MUC17, MAP3K1, CDH1, FAT3, SYNE1, FLG, HMCN1, RYR2 (ryanodine receptor 2), GATA3, MUC4, PIK3CA, KMT2C, TP53, PTEN, ZFHX4, MUC16, and USH2A. Among them, we observed that RYR2 mutation was significantly associated with higher TMB and better clinical prognosis. Moreover, GSEA revealed that RYR2 mutation-enriched signaling pathways were related to immune-associated pathways. Furthermore, based on the CIBERSORT algorithm, we found that RYR2 mutation enhanced the antitumor immune response by enriching CD8+ T cells, activated memory CD4+ T cells, and M1 macrophages. Conclusion. RYR2 is frequently mutated in BC, and its mutation is related to increased TMB and promotes antitumor immunity; thus, RYR2 may serve as a valuable biomarker to predict the immune response. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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20. Research on the Risk Assessment of New Energy Automobile Industry Based on Entropy Weight-Cloud Model in China's Jiangsu Province.
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Cao, Yanqiu, Bian, Yijie, Wang, Rong, and Wang, Lingjun
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ENERGY industries , *AUTOMOBILE industry , *RISK assessment , *ENTROPY (Information theory) , *ENERGY development - Abstract
The development of the new energy vehicle industry is necessary for its advantages of saving energy and reducing greenhouse-gas emissions. However, the industry is currently facing risks with regard to, for example, technology, market, and the policy. Most existing studies of industry risk focused on analyzing and evaluating risk factors and summarizing and interpreting risk phenomena. In this study, systematic classification and quantitative analysis for the risk of the new energy vehicle industry were investigated, in which the entropy weight method and cloud model were combined to evaluate and quantify the industry risk. The formation mechanism of the industry risk from endogenous and exogenous perspectives was analyzed to screen out risk-evaluation factors. Combining the expert-investigation and fuzzy-statistics methods, a risk-evaluation index system with six primary indicators and twenty-four secondary indicators was constructed. On the basis of the entropy weight-cloud model, the risk of the new energy automobile industry of Jiangsu province in July 2019 was evaluated. Results indicated that the impact of exogenous risk on the industry was greater than that of endogenous risk, and industry risk was higher than medium risk, which was close to a higher medium level. A series of suggestions are given for preventing industry risk, such as improving the industry's own ability to resist risk and building the industry's soft environment. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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21. Multiobjective Evaluation of Coevolution among Innovation Populations Based on Lotka–Volterra Equilibrium.
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Wang, Sheng-Yuan, Chen, Wan-Ming, Wang, Rong, and Wu, Xiao-Lan
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GOAL programming , *COEVOLUTION , *EQUILIBRIUM , *SENSITIVITY analysis , *EVALUATION methodology - Abstract
The collaborative evaluation of enterprise innovation populations is a hot issue. The Lotka–Volterra model is a mature method used to evaluate the interaction mechanism of populations and is widely used in innovation ecology research studies. The Lotka–Volterra model mainly focuses on the quantitative characteristics of the interactive populations. The growth mechanisms cannot explain all the synergy mechanisms of the innovative populations. The collaborative evaluation between enterprise innovation populations is a typical multiobjective evaluation problem. The multichoice goal programming model is a mature method to solve multiobjective optimization problems. This paper combines the Lotka–Volterra model and multichoice goal programming method to construct a three-stage multiobjective collaboration evaluation method based on Lotka–Volterra equilibrium. An evaluation example is used to illustrate the application process of this method. The method proposed in this paper has excellent performance in computing, parameter sensitivity analysis, and model stability analysis. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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22. Neferine Exerts Antioxidant and Anti-Inflammatory Effects on Carbon Tetrachloride-Induced Liver Fibrosis by Inhibiting the MAPK and NF-κB/IκBα Pathways.
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Wang, Yuanyuan, Wang, Shaozhan, Wang, Rong, Li, Shengnan, and Yuan, Yongfang
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ANTI-inflammatory agents , *PHOSPHOTRANSFERASES , *ANIMAL experimentation , *EPIDERMAL growth factor , *IMMUNOHISTOCHEMISTRY , *WESTERN immunoblotting , *ISOQUINOLINE , *ANTIOXIDANTS , *FIBROSIS , *LIVER diseases , *HYDROCARBONS , *RATS , *OXIDATIVE stress , *MALONDIALDEHYDE , *CELLULAR signal transduction , *DNA-binding proteins , *PHOSPHORYLATION , *PHARMACODYNAMICS - Abstract
Reversible liver fibrosis is the consequence of diverse liver injuries. Oxidative stress combined with inflammation is the primary cause of carbon tetrachloride- (CCl4-) induced liver fibrosis. Neferine is a bibenzyl isoquinoline alkaloid, which has strong anti-inflammatory and antioxidant properties. The present study attempted to find its antiliver fibrosis effect and explore the potential mechanism to relieve oxidative stress and inflammation in rats with CCl4-induced liver fibrosis. Herein, we found that neferine noticeably mitigated fibrosis and improved liver function. Furthermore, neferine increased the activity of antioxidant enzymes, such as superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and catalase (CAT), but decreased the level of malondialdehyde (MDA). Neferine also decreased the levels of alpha-smooth muscle actin (α-SMA), transforming growth factor β1 (TGF-β1), and inflammatory factors. These results may demonstrate that neferine could effectively inhibit oxidative stress and inflammation in liver fibrosis. To account for the potential mechanism by which neferine relieves oxidative stress and inflammation in liver fibrosis rats, immunohistochemistry analyses and western blotting were performed. The results showed that neferine inhibited the mitogen-activated protein kinase (MAPK) pathway, as evidenced by the reduced phosphorylation of p38 MAPK, ERK 1/2, and JNK. And it inhibited the nuclear factor- (NF-) κB/IκBα pathway, as evidenced by preventing the translocation of NF-κB into nuclei. Our findings indicated a protective role for neferine, acting as an antioxidant and anti-inflammatory agent in CCl4-induced liver fibrosis. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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23. Multiantenna Clustering Collaboration for WPCNs Based on Nonlinear EH.
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Yuan, Lina, Chen, Huajun, Zhou, Anran, Wang, Rong, Wen, Xianli, and Lazaro, Antonio
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OPTIMIZATION algorithms , *ANTENNAS (Electronics) , *ENERGY harvesting , *POWER transmission , *TELECOMMUNICATION systems - Abstract
This article considers a wireless-powered communication network (WPCN) composed of a multiantenna hybrid access point (HAP) based on nonlinear energy harvesting (EH). To improve some distant WDs' throughput performance, one of them is allowed to be selected as a cluster head (CH) to help transfer information from other cluster members (CMs). Nevertheless, the proposed clustering collaboration's performance is essentially restricted by the CH's energy-intensive consumption (EC), which requires to transfer every WDs' information, covering its own. In order to figure out the question, the HAP's energy beamforming (EB) capability with multiple antennas is utilized that can concentrate greater transmission power into the CH to equilibrate its EC to assist other WDs. To be specific, each WD's throughput performance is firstly derived under the proposed approach. A high-efficiency optimization algorithm for addressing cooperative optimization problem is put forward. In addition, the simulations are carried out in the actual network environment, and the results demonstrate that our proposed clustering collaboration with multiple antennas can validly enhance the WPCN's throughput fairness based on nonlinear EH. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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24. Preparation of Cholesteryl-Modified Aminated Pullulan Nanoparticles to Evaluate Nanoparticle of Hydrophobic Degree on Drug Release and Cytotoxicity.
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Yuan, Huabing, Zhong, Wu, Wang, Rong, Zhou, Ping, Nie, Yu, Hu, Wei, Tao, Xiaojun, and Yang, Ping
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INFRARED spectroscopy , *NANOPARTICLES , *LIGHT scattering , *SURFACE properties , *ZETA potential , *FOURIER transforms - Abstract
The formation of nanoparticles (NPs) and surface properties such as size and charge are affected by the amphiphilic property of polymer, which is vital for evaluating their function. Here, we synthesized cholesteryl-modified aminated pullulan polymers (CHPNs) with different amounts of cholesterol succinate (CHS). We characterized the three hydrophobically modified polymers (CHPN1, CHPN2, and CHPN3) (CHS: Pu ‐ N H 2 = 1 / 5 , 2 / 5 , 3 / 5) by Fourier transform infrared spectrometry. Dynamic light scattering (DLS) was used to measure particle size and zeta potential of CHPN NPs. The particle sizes of the three NPs CHPN1, CHPN2, and CHPN3 were 178.0, 144.4, and 97.8 nm, respectively. The particle size was related to the cholesteryl substitution of polymers to a certain extent: the stronger the hydrophobicity, the smaller the particle size. In 48 h, the drug release for CHPN3 and CHPN1 NPs was 57.8% and 72.7%. Thus, the NPs showed good sustained drug release: the greater the degree of hydrophobic substitution, the better the sustained release. The cytotoxicity findings were reversed: CHPN1 NPs, with low hydrophobic substitution, showed the best inhibition of Lewis lung cancer cells. [ABSTRACT FROM AUTHOR]
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- 2020
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25. Design, Synthesis, and Neurotrophic Effect of Arg-Glu-Arg-Met-Ser-(3,5)-Dimethyladamantan-1-Amine In Vitro Evaluations as a Potential NMDAR Antagonist.
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Wang, Xiaozhen, Li, Ze, Zhang, Xiaoyi, Kang, Meimei, Lv, Caizhen, Zhang, Xu, Zhao, Zhiwei, Wu, Yanchuan, Song, Penghui, and Wang, Rong
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SURFACE plasmon resonance , *METHYL aspartate receptors , *ALZHEIMER'S disease , *MOLECULAR dynamics , *RECEPTOR-ligand complexes - Abstract
Methyl-D-aspartate receptor (NMDAR) is an ionotropic glutamate receptor and plays an important role in neuronal degradation of Alzheimer's disease (AD). According to molecular modeling docking studies, we have designed the compound Arg-Glu-Arg-Met-Ser-(3,5)-dimethyladamantan-1-amine (RERMS-MEM), consisting of an AβPP 5-mer peptide (RERMS) and memantine (MEM). This compound could dock into the active sites of N-methyl-D-aspartate receptor type 2B (NMDAR2B) with a −64.14 kcal/mol CDOCKER interaction energy. The stability of RERMS-MEM was evaluated through a 50 ns molecular dynamics simulation. The results revealed that the docked ligand-receptor complex was stable. Furthermore, surface plasmon resonance (SPR) revealed that the RERMS-MEM binding affinity to the NMDAR2B fragment exhibited over 15-fold enhancement compared to MEM. The SH-SY5Y cell assays showed that RERMS-MEM or RERMS at concentrations of 0.1, 1, 10, or 50 μM could enhance the metabolic rate, and MEM showed no difference compared to the control and indicated cytotoxic effects at 50 μM. RERMS-MEM at concentrations of 0.01, 0.1, 1, 10, or 50 μM increased the number of viable cells and reduced the release of lactate dehydrogenase (LDH). RERMS at concentrations of 10 or 50 μM was similar to RERMS-MEM for increasing viable cells, and MEM showed no difference compared to the control and decreased the number of viable cells at 50 μM. RERMS-MEM or RERMS at concentrations of 10 or 50 μM could antagonize Aβ25-35-induced cytotoxicity, and MEM at 50 μM strengthened the cytotoxicity effects. The results revealed that RERMS-MEM showed a strong NMDAR-blocking activity as a potential NMDAR antagonist, enhancing the neurotrophic effect and cellular growth in SH-SY5Y cells. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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26. Neuroprotective Effects of Cerebral Ischemic Preconditioning in a Rat Middle Cerebral Artery Occlusion Model: The Role of the Notch Signaling Pathway.
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Chen, Li, Huang, Kuan, Wang, Rong, Jiang, Qiong, Wu, Zhenghua, Liang, Weidong, Guo, Rui, and Wang, Lifeng
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ANIMAL experimentation , *APOPTOSIS , *CALCIUM-binding proteins , *CELL receptors , *CELLULAR signal transduction , *CEREBRAL arteries , *GENE expression , *RATS , *REPERFUSION , *STEM cells , *TREATMENT effectiveness , *IN vitro studies , *IN vivo studies , *ISCHEMIC preconditioning ,CEREBRAL ischemia treatment - Abstract
Cerebral ischemia-reperfusion (I/R) injury is a major problem worldwide. The Notch signaling pathway plays an important role in neural progenitor cell differentiation and in the inflammatory response after central nervous system injury. This study evaluated whether the neuroprotective effect of cerebral ischemic preconditioning (cIPC) is mediated by the preactivation of the Notch signaling pathway. A rat middle cerebral artery occlusion/reperfusion (MCAO/R) model and glucose deprivation/reoxygenation (OGD/R) cell model were constructed to detect the neuroprotective effects of cIPC. In in vivo experiments, cIPC reduces the neurological functional deficit, cerebral infarction, and cellular apoptosis in the hippocampus induced by middle cerebral artery occlusion/reperfusion (MCAO/R), thus indicating that cIPC can improve neurologic function. Moreover, cIPC can reveal the expression peak of Jagged1, Notch1, NICD, and Hes1 protein, thereby indicating that cIPC can preactivate Notch signaling. However, cIPC-induced improvements in neurologic function are compromised by the γ-secretase inhibitor N-(N-(3,5-difluorophenacetyl)-1-alanyl)-S-phenylglycine t-butyl ester (DAPT). In in vitro experiments, OGD preconditioning (OGDPC) can clearly upregulate Notch1 expression in the OGD/R-treated neuron and neural stem cell. Notch1 pre-overexpression can decrease neuron death and apoptosis under OGD/R treatment. Notch1 pre-overexpression can decrease the percentage of G1 stage cells and increase the percentage of S stage cells in OGD/R-treated neural stem cell. Furthermore, Notch1 pre-knockdown has the opposite effect on cell survival, apoptosis, and cycle in both OGD/R-treated neuron and neural stem cell. In conclusion, our results demonstrate that the neuroprotective effects of cIPC in a rat MCAO/R model are mediated by the preactivation of the Notch signaling pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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27. Prediction and Validation of Hub Genes Associated with Colorectal Cancer by Integrating PPI Network and Gene Expression Data.
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Xiong, Yongfu, You, Wenxian, Wang, Rong, Peng, Linglong, and Fu, Zhongxue
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COLON tumors , *GENE expression , *GENOMICS , *GENETICS ,RECTUM tumors - Abstract
Although hundreds of colorectal cancer- (CRC-) related genes have been screened, the significant hub genes still need to be further identified. The aim of this study was to identify the hub genes based on protein-protein interaction network and uncover their clinical value. Firstly, 645 CRC patients’ data from the Tumor Cancer Genome Atlas were downloaded and analyzed to screen the differential expression genes (DEGs). And then, the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis was performed, and PPI network of the DEGs was constructed by Cytoscape software. Finally, four hub genes (CXCL3, ELF5, TIMP1, and PHLPP2) were obtained from four subnets and further validated in our clinical setting and TCGA dataset. The results showed that mRNA expression of CXCL3, ELF5, and TIMP1 was increased in CRC tissues, whereas PHLPP2 mRNA expression was decreased. More importantly, high expression of CXCL3, ELF5, and TIMP1 was significantly associated with lymphatic invasion, distance metastasis, and advanced tumor stage. In addition, a shorter overall survival was observed in patients with increased CXCL3, TIMP1, and ELF5 expression and decreased PHLPP2 expression. In conclusion, the four hub genes screened by our strategy could serve as novel biomarkers for prognosis prediction of CRC patients. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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28. Potential Targets and Mechanisms of Jiedu Quyu Ziyin Decoction for Treating SLE-GIOP: Based on Network Pharmacology and Molecular Docking.
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Li, Jie, Chang, Run-yu, Chen, Lin-feng, Qian, Su-hai, Wang, Rong-yun, Lan, Ji-le, Huang, Lin, and Ding, Xing-hong
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MOLECULAR docking , *MOLECULAR pharmacology , *IMMUNOREGULATION , *SYSTEMIC lupus erythematosus , *PROTEIN-protein interactions - Abstract
Background. Systemic lupus erythematosus (SLE) is characterized by poor regulation of the immune response leading to chronic inflammation and multiple organ dysfunction. Glucocorticoid (GC) is currently one of the main treatments. However, a high dose or prolonged use of GC may result in glucocorticoid-induced osteoporosis (GIOP). Jiedu Quyu Ziyin decoction (JP) is effective in treating SLE and previous clinical studies have proved that JP can prevent and treat SLE steroid osteoporosis (SLE-GIOP). We aim to examine JPs main mechanism on SLE-GIOP through network pharmacology and molecular docking. Methods. TCMSP and TCMID databases were used to screen potential active compounds and targets of JP. The SLE-GIOP targets are collected from GeneCards, OMIM, PharmGkb, TTD, and DrugBank databases. R software was used to obtain the cross-targets of JP and SLE-GIOP and to perform GO and KEGG enrichment analysis. Cytoscape software was used to make the Chinese Medicines-Active Ingredient-Intersection Targets network diagram. STRING database construct protein–protein interaction network and obtain the core targets. Auto Dock Tools and Pymol software were used for docking. Results. Fifty eight targets overlapped between JP and SLE-GIOP were suggested as potential targets of JP in the treatment of SLE-GIOP. Network topology analysis identified five core targets. GO enrichment analysis was obtained 1,968 items, and the top 10 biological process, closeness centrality, and molecular function were displayed. A total of 154 signaling pathways were obtained by KEGG enrichment analysis, and the top 30 signaling pathways were displayed. JP was well bound by MAPK1, TP53, and MYC according to the molecular docking results. Conclusion. We investigated the potential targets and signaling pathways of JP against SLE-GIOP in this study. It shows that JP is most likely to achieve the purpose of treating SLE-GIOP by promoting the proliferation and differentiation of osteoblasts. A solid theoretical foundation will be provided for the future study of clinical and experimental topics. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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29. High Efficient Construction and Comprehensive Evaluation of Superhydrophobic Filter Screen Coatings.
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Hou, Keke, Meng, Yaowei, Chen, Xinhua, Li, Yanan, and Wang, Rong
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OIL spill cleanup , *CONTACT angle , *SCANNING electron microscopy , *X-ray spectroscopy , *CORROSION resistance , *WEAR resistance , *CORROSION & anti-corrosives - Abstract
The preparation of environment-friendly and efficient superhydrophobic filter screen still faces great challenges. Herein, a compact, solid cerium stearate superhydrophobic-coated stainless steel mesh (SSSM) was prepared by a simple, efficient, and environmentally friendly one-step electrodeposition process. Fourier transform infrared (FTIR) spectroscopy, energy-dispersive X-ray spectroscopy (EDS), scanning electron microscopy (SEM), and contact angle measurement were used to characterize the surface coating (composition, morphology, and contact angle) of SSSM. The results show that the SSSM surface is a dense mastoid cerium stearate micronanostructure coating, and its static contact angle with water can reach 162.73°. In addition, it has excellent self-cleaning performance for conventional simulated pollutants, and the contact angle can still reach 161° after 28 days of storage. In the wear resistance and corrosion resistance tests, the contact angle can still be kept above 150° after 600 mm abrasion under the load of 19.6 kPa, the corrosion current of SSSM is reduced by two orders of magnitude, and its corrosion inhibition efficiency reaches 99.74%. In addition, the oil/water separation performance of SSSM is also very good, and the separation efficiency for different oil/water mixtures exceeds 97%. This study will provide important data support and method reference for the preparation of metal anticorrosion superhydrophobic material coating and the development of environment-friendly and efficient oil–water separation materials. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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30. MultiP-Apo: A Multilabel Predictor for Identifying Subcellular Locations of Apoptosis Proteins.
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Wang, Xiao, Li, Hui, Wang, Rong, Zhang, Qiuwen, Zhang, Weiwei, and Gan, Yong
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CELL death , *PHYSIOLOGICAL effects of proteins , *CELL physiology , *APOPTOSIS , *JACKKNIFE (Statistics) , *PREVENTION - Abstract
Apoptosis proteins play an important role in the mechanism of programmed cell death. Predicting subcellular localization of apoptosis proteins is an essential step to understand their functions and identify drugs target. Many computational prediction methods have been developed for apoptosis protein subcellular localization. However, these existing works only focus on the proteins that have one location; proteins with multiple locations are either not considered or assumed as not existing when constructing prediction models, so that they cannot completely predict all the locations of the apoptosis proteins with multiple locations. To address this problem, this paper proposes a novel multilabel predictor named MultiP-Apo, which can predict not only apoptosis proteins with single subcellular location but also those with multiple subcellular locations. Specifically, given a query protein, GO-based feature extraction method is used to extract its feature vector. Subsequently, the GO feature vector is classified by a new multilabel classifier based on the label-specific features. It is the first multilabel predictor ever established for identifying subcellular locations of multilocation apoptosis proteins. As an initial study, MultiP-Apo achieves an overall accuracy of 58.49% by jackknife test, which indicates that our proposed predictor may become a very useful high-throughput tool in this area. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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31. Reproductive Toxicity of Zishen Yutai Pill in Rats: The Fertility and Early Embryonic Development Study (Segment I).
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Zhou, Li, Huang, Qiuling, Wang, Rong, Zhou, Jie, Ma, Aicui, Chong, Liming, Wu, Yubing, Wang, Yong, Xu, Li, Chen, Ying, Jia, Yuling, Gui, Bo, and Sun, Zuyue
- Subjects
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ANIMAL experimentation , *FERTILITY , *MALE reproductive organs , *HERBAL medicine , *CHINESE medicine , *RATS , *SPERMATOZOA , *SPERM motility , *TOXICITY testing , *FETAL development , *CYCLOPHOSPHAMIDE , *SPERM count , *DRUG administration , *DRUG dosage - Abstract
Purpose. This study was aimed to investigate the reproductive toxicity of Zishen Yutai Pill (ZYP) on fertility and early embryonic development in rats. Methods. SD rats were randomly divided into 5 groups: vehicle control group (distilled water, i.g.), positive control group (80 mg/kg of cyclophosphamide, i.p.), and three ZYP-treated groups (3, 6, and 12 g/kg/d, i.e., 12x, 24x, and 48x clinical doses, i.g.). The high dose was set as the maximum gavage dosage. Results. Cyclophosphamide showed diverse hazards, such as decreased weight of male reproductive organs and sperm density (P<0.05). However, there were no obvious effects of ZYP on physical signs, animal behavior, and survival rate, as well as on weight and food intake during the premating and gestation periods. Importantly, there were no significant adverse effects of ZYP on indexes of copulation, fecundity and fertility indexes, weights and coefficients of male reproductive organs, epididymal sperm number and motility, estrous cycle, preimplantation loss rate, and implantation rate. Besides, the numbers of live and resorbed fetuses per litter were not significantly altered. Conclusions. ZYP had no reproductive toxicities on fertility and early embryonic development in rats at 48x equivalent clinical doses. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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32. Resveratrol Enhances the Radiosensitivity by Inducing DNA Damage and Antitumor Immunity in a Glioblastoma Rat Model under 3 T MRI Monitoring.
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Qian, Liping, Mao, Lihua, Mo, Weixing, Wang, Rong, and Zhang, Yunlong
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BIOLOGICAL models , *ANIMAL experimentation , *GLIOMAS , *MAGNETIC resonance imaging , *RESVERATROL , *RATS , *IMMUNITY , *DNA damage - Abstract
Glioblastoma (GBM) is the most common intracranial tumor with characteristic of malignancy. Resveratrol, a natural originated polyphenolic compound, has been reported to act as a potential radiosensitizer in cancer therapy. Magnetic resonance imaging (MRI) is the first choice for the diagnosis, pathological grading, and efficacy evaluation of GBM. In this study, MRI was applied to observe whether resveratrol could intensify the anti-GBM tumor effect by enhancing antitumor immunity during radiotherapy. We established an intracranial C6 GBM model in SD rats, treated with radiation and resveratrol. The increased body weight, the inhibition on mortality, and tumor volume in radiated- GBM rats were further enhanced by resveratrol addition, while the pathological damage of brain was alleviated. The modulation of radiation on inflammation, cell cycle, and apoptosis was strengthened by resveratrol; and Ki-67, PD-L1, and cell cycle- and apoptosis-related protein expressions were also improved by cotreatment. Besides, cotreatment attenuated DNA damage and induced G0/G1-phase cell arrest of GBM rats, accompanied with the changed expression of ATM-AKT-STAT3 pathway-related proteins. Moreover, the percentages of CD3+CD8+T cells and IFN-γ+CD8+T cells were enhanced, while (CD4+CD25+Foxp3)/CD4+T cells were decreased by radiation or resveratrol, which was strengthened by cotreatment. The modulation effect of cotreatment on CD3, Foxp3, and IFN-γ levels was also stronger than radiation or resveratrol alone. To conclude, resveratrol enhanced the effect of radiotherapy by inducing DNA damage and antitumor immunity in the intracranial C6 GBM. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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33. Experimental Study on Shield Tunneling Control in Full Section Water-Rich Sand Layer of Collapsible Loess.
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Mei, Yuan, Zhang, Xinyue, Zhang, Shumin, Wang, Rong, Yang, Tong, and Zhang, Yuhang
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TUNNEL design & construction , *LOESS , *BENTONITE , *SAND , *RAILROAD crossings , *GROUTING , *SLURRY - Abstract
Taking China's first application of domestic shield tunneling in a water-rich sand layer for crossing a high-speed railway as research background, the influence of changes in the shield tunneling parameters on ground settlement is analyzed based on field tests, and a construction control method suitable for shield tunneling under a risk source in a water-rich sand layer is proposed. The test results show that the use of 10% sodium bentonite as the soil modifier for soil pressure balance shield tunneling into the water-rich sand layer has advantages, while adding 50% loess into the bentonite slurry in the gravel sand layer can greatly improve the impermeability of the soil; the settlement of soil can be effectively reduced by using the special segment with grouting holes for deep grouting and applying the adaptive transformation of the shield cutter. Based on the statistical analysis results, a reasonable range for the cutter head torque, cutter head speed, chamber pressure, bentonite injection volume, and advanced speed in the water-rich sand layer can provide a construction control basis for similar projects and provide data support for the compilation of relevant specifications. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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34. Orthodontic Rubber Band-Assisted Endoscopic Submucosal Dissection: An Efficient Method for Treating Superficial Colorectal Tumors.
- Author
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Zheng, Linfu, Wang, Wen, Li, Dazhou, Chen, Junguo, Chen, Longping, Wang, Rong, Jiang, Chuanshen, Zhang, Guanpo, Hou, Yaping, Zheng, Jin, and Bai, Yang
- Subjects
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COLON tumors , *PROPENSITY score matching , *RUBBER , *DISSECTION , *COLORECTAL cancer , *ORTHOPEDIC traction , *CORRECTIVE orthodontics - Abstract
Background. Colorectal endoscopic submucosal dissection (ESD) is a complex operation. Effective traction is crucial. We have successfully used an orthodontic rubber band (ORB) combined with the clip traction method to assist ESD (ORB-ESD). The aim of this retrospective study is to describe the method and to compare the efficacy and safety of ORB-ESD versus conventional ESD in the treatment of superficial colorectal tumors. Methods. We retrospectively analyzed the data of patients with superficial colorectal tumor (with diameter ≥ 20 mm) who received either ORB-ESD (n = 34) or conventional ESD (n = 90) between January 2019 and September 2020. Propensity score matching (PSM) was used to match the clinical data of 31 pairs of patients in each group. Results. Operation time was significantly shorter for ORB-ESD than for conventional ESD (34.5 minutes vs. 56 minutes, P ≤ 0.001). In the propensity-matched cohorts, the operation time remained significantly shorter in the ORB-ESD patients (35 minutes vs. 50 minutes, P = 0.001). Postoperative adverse events, en bloc resection rate, and R0 resection rate were comparable between the two groups (P > 0.05), both before and after propensity score matching. In the ORB subgroup analysis, the trainee and expert ESD operation times were similar (37 (26–53) vs. 33.5 (26–37) minutes, respectively; P = 0.274). Conclusion. ORB-ESD appears to be an effective technique for ESD of colorectal cancer. Our findings need to be confirmed in large prospective multicenter studies. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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35. A hybrid cooperative navigation method for UAV swarm based on factor graph and Kalman filter.
- Author
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Chen, Mingxing, Xiong, Zhi, Xiong, Jun, and Wang, Rong
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AERONAUTICAL navigation , *KALMAN filtering , *GLOBAL Positioning System , *MONTE Carlo method - Abstract
Navigation plays an important role in the task execution of the micro-unmanned aerial vehicle (UAV) swarm. The Cooperative Navigation (CN) method that fuses the observation of onboard sensors and relative information between UAVs is a research hotspot. Aiming at the efficiency and accuracy problems of previous studies, this article proposes a hybrid-CN method for UAV swarm based on Factor Graph and Kalman filter. A global Factor Graph is used to combine Global Navigation Satellite System (GNSS) and ranging information to provide position estimations for modifying the distributed Kalman filter; distributed Kalman filter is established on each UAV to fuse inertial information and optimized position estimation to modify the navigation states. In order to provide time-consistent GNSS position information for the Factor Graph, a time synchronization filter is designed. The proposed method is tested and verified using standard Monte Carlo simulations, simulation results show that it can provide a more precise and efficient CN solution than traditional CN methods. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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- View/download PDF
36. Investigation of the Mechanisms of Chuankezhi Injection in the Treatment of Asthma Based on the Network Pharmacology Approach.
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Zhu, Hao, Shi, Yuhuan, Jiang, Shanshan, Jiao, Xiuxiu, Zhu, Hui, Wang, Rong, and Yuan, Yongfang
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DRUG therapy for asthma , *CYTOKINES , *HERBAL medicine , *INJECTIONS , *FLAVONOIDS , *PHARMACOLOGY , *GROWTH factors , *CELLULAR signal transduction , *DNA-binding proteins , *TRANSFERASES , *CHEMOKINES , *CHINESE medicine , *DRUG administration , *DRUG dosage - Abstract
Background. Chuankezhi injection (CKZI) was an effective traditional Chinese medicine (TCM) injection in adjuvant bronchial asthma therapy. In this report, we used a network pharmacology method to reveal the mechanisms of CKZI for the treatment of asthma. Methods. The candidate compounds in CKZI were determined by searching the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and China National Knowledge Infrastructure website (CNKI). The targets of candidate compounds were searched in the TCMSP, DrugBank 5.0, and SwissTargetPrediction. The disease targets were screened from the Online Mendelian Inheritance in Man (OMIM) and GeneCards. The overlapping gene symbols between candidate compounds and disease were filtered via a Venn diagram and were considered as potential targets. A protein-protein interaction (PPI) network and disease-related candidate compound-target-pathway (DC-T-P) network were visualized by Cytoscape 3.6.1. Gene Ontology (GO) functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed by metascape to determine the pathways related to asthma. Results. A total of 70 overlapping gene symbols were recognized as potential targets. Cytokines (IL6, TNF, and IL1B) and chemokines (CXCL8 and CCL2) could be recognized as hub genes. Asthma-related candidate compounds were mainly flavonoids, such as quercetin, luteolin, and kaempferol. The cytokine-mediated signaling pathway, cytokine receptor binding, and membrane craft were the most significant biological process (BP), molecular function (MF), and cellular component (CC) of GO function results, respectively. The relevant pathways of CKZI against asthma mainly include IL-17, NF-kappa B, HIF-1, calcium, and PI3K-Akt signaling pathways. Conclusion. Our research provided a theoretical basis for further investigating the mechanisms of CKZI in the treatment of asthma. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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37. Molecular Targets and Mechanisms of Scutellariae radix-Coptidis rhizoma Drug Pair for the Treatment of Ulcerative Colitis Based on Network Pharmacology and Molecular Docking.
- Author
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Niu, Kai, Li, Qifang, Liu, Yuan, Qiao, Yi, Li, Bingbing, Wei, Chao, Wang, Kunrui, Cui, Lu'an, Zheng, Canlei, Wang, Rong, Zhang, Li, Zhang, Honghua, Sun, Bing, and Yu, Bin
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ULCERATIVE colitis , *INTERLEUKINS , *LIPOPOLYSACCHARIDES , *MEDICINAL plants , *GINGER , *COMBINATION drug therapy , *PHARMACOLOGY , *MOLECULAR biology , *CELLULAR signal transduction , *GENE expression , *TUMOR necrosis factors , *MOLECULAR structure , *CHINESE medicine - Abstract
This study aims to analyze the targets of the effective active ingredients of Scutellariae radix-Coptidis rhizoma drug pair (SCDP) in ulcerative colitis (UC) by network pharmacology and molecular docking and to explore the associated therapeutic mechanism. The effective active ingredients and targets of SCDP were determined from the TCMSP database, and the drug ingredient-target network was constructed using the Cytoscape software. The disease targets related to UC were searched in GeneCards, DisGeNET, OMIM, and DrugBank databases. Then, the drug ingredient and disease targets were intersected to construct a protein-protein interaction network through the STRING database. The Metascape database was used for the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of the predicted targets of SCDP for UC. The Autodock software was used for molecular docking between the main active ingredient and the core target to evaluate the binding ability. SCDP has 43 effective active ingredients and 134 intersection targets. Core targets included AKT1, TP53, IL-6, VEGFA, CASP3, JUN, TNF, MYC, EGFR, and PTGS2. GO functional enrichment analysis showed that biological process was mainly associated with a cytokine-mediated signaling pathway, response to an inorganic substance, response to a toxic substance, response to lipopolysaccharide, reactive oxygen species metabolic process, positive regulation of cell death, apoptotic signaling pathway, and response to wounding. KEGG enrichment analysis showed main pathway concentrations were related to pathways in cancer, AGE-RAGE signaling pathway in diabetic complications, bladder cancer, IL-17 signaling pathway, apoptosis, p53 signaling pathway, and PI3K-Akt signaling pathway. The drug active ingredient-core target-key pathway network contains 41 nodes and 108 edges, of which quercetin, wogonin, baicalein, acacetin, oroxylin A, and beta-sitosterol are important active ingredients; PTGS2, CASP3, TP53, IL-6, TNF, and AKT1 are important targets; and the pathways involved in UC treatment include pathways in cancer, PI3K-Akt signaling pathway, AGE-RAGE signaling pathway in diabetic, apoptosis, IL-17 signaling pathway and herpes simplex infection. The active ingredient has a good binding capacity to the core target. SCDP key active ingredients are mainly quercetin, wogonin, baicalein, acacetin, oroxylin A, and beta-sitosterol, which function mainly by regulating targets, such as PTGS2, CASP3, TP53, IL-6, TNF, and AKT1, and are associated with multiple signaling pathways as pathways in cancer, PI3K-Akt signaling pathway, apoptosis, IL-17 signaling pathways. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
38. Based on Network Pharmacology to Explore the Molecular Targets and Mechanisms of Gegen Qinlian Decoction for the Treatment of Ulcerative Colitis.
- Author
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Wei, Meiqi, Li, He, Li, Qifang, Qiao, Yi, Ma, Qun, Xie, Ruining, Wang, Rong, Liu, Yuan, Wei, Chao, Li, Bingbing, Zheng, Canlei, Sun, Bing, and Yu, Bin
- Subjects
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CELLULAR signal transduction , *COMPUTER software , *GENES , *HERBAL medicine , *CHINESE medicine , *METABOLISM , *MOLECULAR structure , *ULCERATIVE colitis , *PLANT extracts , *MOLECULAR dynamics , *FLAVONOLS , *DESCRIPTIVE statistics , *MOLECULAR docking - Abstract
Background. Gegen Qinlian (GGQL) decoction is a common Chinese herbal compound for the treatment of ulcerative colitis (UC). In this study, we aimed to identify its molecular target and the mechanism involved in UC treatment by network pharmacology and molecular docking. Material and Methods. The active ingredients of Puerariae, Scutellariae, Coptis, and Glycyrrhiza were screened using the TCMSP platform with drug ‐ like properties DL ≥ 0.18 and oral availability OB ≥ 30 %. To find the intersection genes and construct the TCM compound-disease regulatory network, the molecular targets were determined in the UniProt database and then compared with the UC disease differential genes with P value < 0.005 and ∣ log 2 fold change ∣ > 1 obtained in the GEO database. The intersection genes were subjected to protein-protein interaction (PPI) construction and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. After screening the key active ingredients and target genes, the AutoDock software was used for molecular docking, and the best binding target was selected for molecular docking to verify the binding activity. Results. A total of 146 active compounds were screened, and quercetin, kaempferol, wogonin, and stigmasterol were identified as the active ingredients with the highest associated targets, and NOS2, PPARG, and MMP1 were the targets associated with the maximum number of active ingredients. Through topological analysis, 32 strongly associated proteins were found, of which EGFR, PPARG, ESR1, HSP90AA1, MYC, HSPA5, AR, AKT1, and RELA were predicted targets of the traditional Chinese medicine, and PPARG was also an intersection gene. It was speculated that these targets were the key to the use of GGQL in UC treatment. GO enrichment results showed significant enrichment of biological processes, such as oxygen levels, leukocyte migration, collagen metabolic processes, and nutritional coping. KEGG enrichment showed that genes were particularly enriched in the IL-17 signaling pathway, AGE-RAGE signaling pathway, toll-like receptor signaling pathway, tumor necrosis factor signaling pathway, transcriptional deregulation in cancer, and other pathways. Molecular docking results showed that key components in GGQL had good potential to bind to the target genes MMP3, IL1B, NOS2, HMOX1, PPARG, and PLAU. Conclusion. GGQL may play a role in the treatment of ulcerative colitis by anti-inflammation, antioxidation, and inhibition of cancer gene transcription. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
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39. Brain Functional Network in Chronic Asymptomatic Carotid Artery Stenosis and Occlusion: Changes and Compensation.
- Author
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He, Shihao, Liu, Ziqi, Xu, Zongsheng, Duan, Ran, Yuan, Li, Xiao, Chu, Yi, Zhe, and Wang, Rong
- Subjects
- *
CEREBRAL hemispheres , *CEREBRAL ischemia , *MAGNETIC resonance , *NEUROPSYCHOLOGICAL tests ,CAROTID artery stenosis - Abstract
Asymptomatic carotid artery stenosis (CAS) and occlusion (CAO) disrupt cerebral hemodynamics. There are few studies on the brain network changes and compensation associated with the progression from chronic CAS to CAO. In the current study, our goal is to improve the understanding of the specific abnormalities and compensatory phenomena associated with the functional connection in patients with CAS and CAO. In this prospective study, 27 patients with CAO, 29 patients with CAS, and 15 healthy controls matched for age, sex, education, handedness, and risk factors underwent neuropsychological testing and resting-state functional magnetic resonance (rs-fMRI) imaging simultaneously; graph theoretical analysis of brain networks was performed to determine the relationship between changes in brain network connectivity and the progression from internal CAS to CAO. The global properties of the brain network assortativity (p = 0.002), hierarchy (p = 0.002), network efficiency (p = 0.011), and small-worldness (p = 0.009) were significantly more abnormal in the CAS group than in the control and CAO groups. In patients with CAS and CAO, the nodal efficiency of key nodes in multiple brain regions decreased, while the affected hemisphere lost many key functional connections. In this study, we found that patients with CAS showed grade reconstruction, invalid connections, and other phenomena that impaired the efficiency of information transmission in the brain network. A compensatory functional connection in the contralateral cerebral hemisphere of patients with CAS and CAO may be an important mechanism that maintains clinical asymptomatic performance. This study not only reveals the compensation mechanism of cerebral hemisphere ischemia but also validates previous explanations for brain function connectivity, which can help provide interventions in advance and reduce the impairment of higher brain functions. This trial is registered with Clinical Trial Registration-URL http://www.chictr.org.cn and Unique identifier ChiCTR1900023610. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
40. The Diagnosis Performance of the TCM Syndromes of Irritable Bowel Syndrome by Gastroenterologists Based on Modified Simple Criteria Compared to TCM Practitioners: A Prospective, Multicenter Preliminary Study.
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Song, Jun, Yang, Ling, Su, Shuai, Piao, Mei-Yu, Li, Bao-Li, Liang, Lie-Xin, Zuo, Guo-Wen, Tang, Zhi-Min, Long, Yan-qin, Chen, Xiao-Li, Dai, Ning, Mo, Jian-Ling, Yu, Yue, Yu, Wen-Yong, Zhang, Mei, Wang, Rong-Quan, Chen, Jing, and Hou, Xiao-Hua
- Subjects
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IRRITABLE colon diagnosis , *CLINICAL competence , *GASTROENTEROLOGISTS , *LONGITUDINAL method , *MEDICAL cooperation , *CHINESE medicine , *PROFESSIONS , *RESEARCH , *DESCRIPTIVE statistics , *TERTIARY care - Abstract
Purpose. Traditional Chinese medicine (TCM) including Chinese patent medicine has been widely used to treat irritable bowel syndrome (IBS). Syndrome differentiation is the essence of TCM. However, the diagnostic ability of gastroenterologists to detect TCM syndromes in IBS in China remains unknown. The aim of this study was to investigate the ability of gastroenterologists to diagnose the TCM syndromes of IBS based on modified simple criteria compared with TCM practitioners. Methods. Patients meeting the Rome III criteria for IBS-D or IBS-C were recruited from six tertiary referral centers between January 2016 and December 2017. After learning the diagnosis criteria of the TCM syndromes in IBS, gastroenterologists first diagnosed the syndromes of the enrolled patients. Subsequently, the patients were diagnosed by TCM practitioners. The rate of agreement between the gastroenterologists and TCM practitioners was analyzed. In addition, demographic data and the distribution of TCM syndrome types in IBS were also analyzed. Results. A total of 178 patients (93 males and 85 females), including 131 patients with IBS-D and 47 patients with IBS-C, were enrolled in this study. The rate of agreement of the syndrome diagnosis between the gastroenterologists and TCM practitioners was 84.3%. The diagnosis consistency rates among IBS-D patients and IBS-C patients were 87.0% and 76.5%, respectively. The most common TCM syndrome type in IBS-D patients was liver depression and spleen deficiency syndrome (27.5%), followed by spleen-yang deficiency syndrome (19.8%). Dryness and heat in intestine syndrome was the most common TCM syndrome in IBS-C patients (57.4%). Conclusions. Gastroenterologists had good diagnostic agreement with TCM practitioners for diagnosing TCM syndrome types in IBS after learning the diagnostic criteria. This knowledge can aid gastroenterologists in selecting suitable Chinese patent medicine to treat IBS. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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41. Blockchain-Based Intelligent Transportation: A Sustainable GCU Application System.
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Du, Xiaomin, Gao, Yang, Wu, Chia-Huei, Wang, Rong, and Bi, Datian
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SUSTAINABLE transportation , *INTELLIGENT transportation systems , *BLOCKCHAINS , *SET theory , *SOCIAL problems - Abstract
The purpose of this study is to explore how to apply blockchain technology to intelligent transportation, create a hierarchical theoretical framework of intelligent transportation, and explore a sustainable application system of intelligent transportation under the blockchain. However, not only this hierarchical theoretical framework must consider unnecessary attributes and the interrelationships between the aspects and the criteria, but also the sustainable application system must be in consideration in multiple stakeholders. Hence, fuzzy set theory is used for screening out the unnecessary attributes, a decision-making trial and evaluation laboratory (DEMATEL) is proposed to manage the complex interrelationships among the aspects and attributes, and interpretive structural modeling (ISM) is used to divide the hierarchy and construct a hierarchical theoretical framework. Finally, the research develops a sustainable GCU application system for intelligent transportation under the blockchain. The results show that (1) solving social problems is the primary link, (2) economic tasks are mainly focused on smart contracts and affected by the social problems, (3) the continuous improvement of environmental issues requires a solution to social problems, and (4) the application system of blockchain in intelligent transportation needs to be built from three levels including the government layer, the company layer, and the user layer. This theoretical hierarchical framework aims to guide intelligent transportation toward the application of blockchain. This study also proposes the engagement of stakeholders for establishing a sustainable application system. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
42. Relationship between Urinary Alzheimer-Associated Neuronal Thread Protein and Apolipoprotein Epsilon 4 Allele in the Cognitively Normal Population.
- Author
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Li, Yuxia, Kang, Meimei, Sheng, Can, Chen, Guanqun, Li, Taoran, Wang, Jun, Cai, Yanning, Wang, Rong, and Han, Ying
- Subjects
- *
ALLELES , *CORONARY disease , *HEART diseases , *THREAD , *PROTEINS - Abstract
We investigated the relationship between urinary Alzheimer-associated neuronal thread protein (AD7c-NTP) levels and apolipoprotein epsilon 4 (ApoE ɛ4) alleles, as well as other factors that cause cognitive decline, in the cognitively normal population. We recruited 329 cognitively normal right-handed Han Chinese subjects who completed ApoE gene testing and urinary AD7c-NTP testing. There was no significant difference in urinary AD7c-NTP levels between the normal control and subjective cognitive decline groups. Urinary AD7c-NTP levels were significantly higher in subjects with ApoE ɛ3/4 and 4/4 [0.6074 (0.6541) ng/mL] than in subjects without ApoE ɛ4 [0.4368 (0.3392) ng/mL and 0.5287 (0.3656) ng/mL], and urinary AD7c-NTP levels positively correlated with ApoE genotype grade (r = 0.165 , p = 0.003). There were significant differences in urinary AD7c-NTP levels between subjects with and without a history of coronary heart disease or diabetes. Urinary AD7c-NTP levels were not related to years of education, nature of work, family history of dementia, a history of hypertension, stroke, anemia, or thyroid dysfunction. Urinary AD7c-NTP levels were positively correlated with ApoE grade in the cognitively normal population. The relationship between risk factors of cognitive decline and urinary AD7c-NTP levels provides a new way for us to understand AD and urinary AD7c-NTP. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
43. Amino Acid Profiling Study of Psidium guajava L. Leaves as an Effective Treatment for Type 2 Diabetic Rats.
- Author
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Xu, Chang, Li, Xin, Zeng, Debin, Liu, Ying, Gao, Yuhang, Tsunoda, Makoto, Deng, Shiming, Xie, Xi, Wang, Rong, Li, Lu-shuang, Song, Yanting, and Zhang, Yingxia
- Subjects
- *
AMINO acids , *AMINOGLYCOSIDES , *ANIMAL experimentation , *BLOOD sugar , *DISCRIMINANT analysis , *GUAVA , *LEAVES , *LEUCINE , *LIPIDS , *METABOLISM , *METHIONINE , *TYPE 2 diabetes , *PHENYLALANINE , *RATS , *TRYPTOPHAN , *TYROSINE , *VALINE , *CYSTEINE , *ISOLEUCINE - Abstract
Type 2 diabetes mellitus (T2DM) has become a major disease threatening human health worldwide. At present, the treatment of T2DM cannot cure diabetes and is prone to many side effects. Psidium guajava L. leaves have been reported to possess hypoglycemic activity, and they have been widely used in diabetes treatment in the folk. However, the antidiabetic mechanism has not been clearly explained. Also, the change in amino acid profile can reflect a metabolic disorder and provide insights into system-wide changes in response to physiological challenges or disease processes. The study found that P. guajava L. leaves can decrease fasting blood glucose and lipid levels in type 2 diabetic rats induced by streptozotocin. Through the analysis of amino acid profiling following 20 days of gavage administration, the concentration data were modeled by principal component analysis and orthogonal partial least squares discriminant analysis to find the different metabolites and related metabolic pathways (including cysteine and methionine metabolism, valine, leucine, and isoleucine biosynthesis, phenylalanine, tyrosine, and tryptophan biosynthesis) for the explanation of the hypoglycemic mechanism of P. guajava L., which provides an experimental and theoretical basis for diabetes prediction and for the development of new drugs for the treatment of diabetes. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
44. Interaction of Mitoxantrone-Loaded Cholesterol Modified Pullulan Nanoparticles with Human Serum Albumin and Effect on Drug Release.
- Author
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Yuan, Liming, Guo, Bu, Zhong, Wu, Nie, Yu, Yao, Xiaoyan, Peng, Xiaofeng, Wang, Rong, Yu, Hongyuan, Yang, Shanyi, He, Chunlian, Tao, Xiaojun, and Zhang, Qiufang
- Subjects
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PHARMACOLOGY , *ISOTHERMAL titration calorimetry , *SERUM albumin , *CIRCULAR dichroism , *FLUORESCENCE spectroscopy , *TRANSMISSION electron microscopy - Abstract
To clarify nanoparticle-protein interaction and their action characteristics, the interactions between MTO-CHP NPs and human serum albumin (HSA) were studied by isothermal titration calorimetry (ITC), fluorescence spectroscopy, dynamic light scattering (DLS), and circular dichroism spectroscopy (CD). Hydrophobically modified pullulan (CHP) nanoparticles (NPs) loaded with mitoxantrone (MTO) were prepared (MTO-CHP NPs) with size 166.9 nm. The spherical shape was verified by transmission electron microscopy (TEM). The ITC results demonstrated an interaction between MTO-CHP NPs mainly by hydrophobic interaction force, electrostatic force, and hydrogen bonding. The mean binding constant KA was 0.832×104 M−1 and mean HSA coverage 0.939±0.302. MTO-CHP NPs could quench the fluorescence intensity of HSA, which gradually decreased to be balanced in 9 h and indicated the completion of the complexation. The size and zeta potential changes of the combined particle were dynamically detected with DLS at 0, 3, 6, 9, 12, 15, and 18 h. When the reaction was completed at 9 h, the particle size and potential remained stable, accompanied by a size change from 89.91 to about 145 nm and potential change from -15 to -3 mV, respectively. The results of CD measurement showed that the change in ellipticity of HSA at 208 nm was similar to the fluorescence spectra and DLS measurements with MTO-CHP NPs combined with HSA. At the beginning of the reaction, the proportion of α-helix was 52.3% to 43.7%, which decreased by 39.1% at compound stabilization. The release of MTO from MTO-CHP NPs at pH=5.6 was significantly accelerated, whereas that of MTO from HSA-MTO-CHP NPs was significantly reduced, and the drug release was significantly slowed down even under acidic conditions, which indicates the beneficial effect of HSA on the persistence and stability of the HSA-MTO-CHP NP compound. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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45. Correlation between Soluble α-Klotho and Renal Function in Patients with Chronic Kidney Disease: A Review and Meta-Analysis.
- Author
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Wang, Qinglian, Su, Wenyan, Shen, Zhenwei, and Wang, Rong
- Subjects
- *
KIDNEY physiology , *CHRONIC kidney failure , *ONLINE information services , *PUBLICATION bias , *STATISTICS , *MEDICAL information storage & retrieval systems , *CONFIDENCE intervals , *META-analysis , *KIDNEY function tests , *SYSTEMATIC reviews , *STATISTICAL correlation , *MEDLINE , *DATA analysis - Abstract
Objective. Over decades, numerous inconsistent studies are reported on the relationship between soluble α-Klotho and renal function in patients with chronic kidney disease (CKD). This study aims to perform a meta-analysis to figure out the correlations between soluble α-Klotho and renal function in patients with CKD. Materials and Methods. We searched medical and scientific literature databases, PubMed and EMBASE (from the inception to October 2017), for publications that reported studies on associations between soluble α-Klotho and renal function in patients with CKD. Only publications in English were extracted. Summary correlation coefficient (r) values were extracted from each study, and 95% confidence intervals (CIs) were calculated. Publication bias was tested, and sensitivity and subgroup analyses were performed to investigate potential heterogeneity. Results. Of 611 studies, 9 publications with 1457 patients were included into the analysis. The following data were extracted from the literature: first author, year of publication, research region, research index, sample size, average age and Pearson or Spearman correlation coefficient, study design, the αKlotho/FGF23 assays utilized, full length, or the C-terminal fragment of FGF23. The pooled r between α-Klotho and estimated glomerular filtration rate (eGFR), FGF-23 were 0.35 (95%CI, 0.23~0.46, and P<0.05), -0.10 (95%CI, -0.19~-0.01, and P<0.05) with remarkable significance, indicating moderate heterogeneity. There was no significant heterogeneity between subgroups in analyses of α-Klotho and eGFR stratified by research region, mean age, and eGFR, but heterogeneity exists in analyses of α-Klotho and FGF-23 stratified by research region. There was no significant correlation between a-klotho and Ca and PTH and PHOS. There was no evidence of publication bias with Egger’s test (p=0.360) or with Begg’s test (p=0.902) and the distribution of funnel plots was symmetrical in all of our analysis. Conclusions. There exists a significant positive correlation between soluble α-Klotho and eGFR in patients with CKD. Also, a significant negative correlation between α-Klotho and FGF23 levels is proven. This raises hope to employ αKlotho and FGF23 as early biomarkers of CKD. However, further large prospective follow-up researches are needed to validate this hypothesis and to explore whether maintaining or elevating the Klotho level could improve renal function and complications in CKD patients. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
46. Predicting Subcellular Localization of Apoptosis Proteins Combining GO Features of Homologous Proteins and Distance Weighted KNN Classifier.
- Author
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Wang, Xiao, Li, Hui, Zhang, Qiuwen, and Wang, Rong
- Subjects
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ALGORITHMS , *AMINO acids , *APOPTOSIS , *STATISTICAL correlation , *GENETIC research , *PROTEINS , *RESEARCH funding , *DESCRIPTIVE statistics ,RESEARCH evaluation - Abstract
Apoptosis proteins play a key role in maintaining the stability of organism; the functions of apoptosis proteins are related to their subcellular locations which are used to understand the mechanism of programmed cell death. In this paper, we utilize GO annotation information of apoptosis proteins and their homologous proteins retrieved from GOA database to formulate feature vectors and then combine the distance weighted KNN classification algorithm with them to solve the data imbalance problem existing in CL317 data set to predict subcellular locations of apoptosis proteins. It is found that the number of homologous proteins can affect the overall prediction accuracy. Under the optimal number of homologous proteins, the overall prediction accuracy of our method on CL317 data set reaches 96.8% by Jackknife test. Compared with other existing methods, it shows that our proposed method is very effective and better than others for predicting subcellular localization of apoptosis proteins. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
47. Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro.
- Author
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Lv, Zhimei, Hu, Mengsi, Ren, Xiaoxu, Fan, Minghua, Zhen, Junhui, Chen, Liqun, Lin, Jiangong, Ding, Nannan, Wang, Qun, and Wang, Rong
- Subjects
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GLUCOSE in the body , *CYTOSKELETON , *PROTEIN-tyrosine kinases , *PHOSPHORYLATION , *CELLULAR signal transduction , *IN vitro studies - Abstract
Fyn, a member of the Src family of tyrosine kinases, is a key regulator in cytoskeletal remodeling in a variety of cell types. Recent studies have demonstrated that Fyn is responsible for nephrin tyrosine phosphorylation, which will result in polymerization of actin filaments and podocyte damage. Thus detailed involvement of Fyn in podocytes is to be elucidated. In this study, we investigated the potential role of Fyn/ROCK signaling and its interactions with paxillin. Our results presented that high glucose led to filamentous actin (F-actin) rearrangement in podocytes, accompanied by paxillin phosphorylation and increased cell motility, during which Fyn and ROCK were markedly activated. Gene knockdown of Fyn by siRNA showed a reversal effect on high glucose-induced podocyte damage and ROCK activation; however, inhibition of ROCK had no significant effects on Fyn phosphorylation. These observations demonstrate that in vitro Fyn mediates high glucose-induced actin cytoskeleton remodeling of podocytes via promoting ROCK activation and paxillin phosphorylation. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
48. Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro.
- Author
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Lv, Zhimei, Hu, Mengsi, Ren, Xiaoxu, Fan, Minghua, Zhen, Junhui, Chen, Liqun, Lin, Jiangong, Ding, Nannan, Wang, Qun, and Wang, Rong
- Subjects
- *
SRC gene , *PROTEIN-tyrosine kinases , *GLUCOSE , *CYTOSKELETON , *CELLULAR signal transduction , *PHOSPHORYLATION , *IN vitro studies - Abstract
Fyn, a member of the Src family of tyrosine kinases, is a key regulator in cytoskeletal remodeling in a variety of cell types. Recent studies have demonstrated that Fyn is responsible for nephrin tyrosine phosphorylation, which will result in polymerization of actin filaments and podocyte damage. Thus detailed involvement of Fyn in podocytes is to be elucidated. In this study, we investigated the potential role of Fyn/ROCK signaling and its interactions with paxillin. Our results presented that high glucose led to filamentous actin (F-actin) rearrangement in podocytes, accompanied by paxillin phosphorylation and increased cell motility, during which Fyn and ROCK were markedly activated. Gene knockdown of Fyn by siRNA showed a reversal effect on high glucose-induced podocyte damage and ROCK activation; however, inhibition of ROCK had no significant effects on Fyn phosphorylation. These observations demonstrate that in vitro Fyn mediates high glucose-induced actin cytoskeleton remodeling of podocytes via promoting ROCK activation and paxillin phosphorylation. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
49. Luoyutong Treatment Promotes Functional Recovery and Neuronal Plasticity after Cerebral Ischemia-Reperfusion Injury in Rats.
- Author
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Wang, Ning-qun, Wang, Li-ye, Zhao, Hai-ping, Liu, Ping, Wang, Rong-liang, Song, Jue-xian, Gao, Li, Ji, Xun-ming, and Luo, Yu-min
- Abstract
Luoyutong (LYT) capsule has been used to treat cerebrovascular diseases clinically in China and is now patented and approved by the State Food and Drug Administration. In this retrospective validation study we investigated the ability of LYT to protect against cerebral ischemia-reperfusion injury in rats. Cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion followed by reperfusion. Capsule containing LYT (high dose and medium dose) as treatment group and Citicoline Sodium as positive control treatment group were administered daily to rats 30 min after reperfusion. Treatment was continued for either 3 days or 14 days. A saline solution was administered to control animals. Behavior tests were performed after 3 and 14 days of treatment. Our findings revealed that LYT treatment improved the neurological outcome, decreased cerebral infarction volume, and reduced apoptosis. Additionally, LYT improved neural plasticity, as the expression of synaptophysin, microtubule associated protein, and myelin basic protein was upregulated by LYT treatment, while neurofilament 200 expression was reduced. Moreover, levels of brain derived neurotrophic factor and basic fibroblast growth factor were increased. Our results suggest that LYT treatment may protect against ischemic injury and improve neural plasticity. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
50. Autocrine Human Urotensin II Enhances Macrophage-Derived Foam Cell Formation in Transgenic Rabbits.
- Author
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Zhao, Sihai, Li, Yafeng, Gao, Shoucui, Wang, Xiaojing, Sun, Lijing, Cheng, Daxing, Bai, Liang, Guan, Hua, Wang, Rong, Fan, Jianglin, and Liu, Enqi
- Subjects
- *
GLUCOSE analysis , *ADIPOSE tissues , *ANIMAL experimentation , *ATHEROSCLEROSIS , *BIOLOGICAL models , *CELL physiology , *CHOLESTEROL , *COMPARATIVE studies , *CHOLESTEROL content of food , *GENETIC techniques , *HIGH density lipoproteins , *LOW density lipoproteins , *MACROPHAGES , *PEPTIDE hormones , *RABBITS , *SMOOTH muscle , *TRIGLYCERIDES , *DESCRIPTIVE statistics - Abstract
Circulating urotensin II (UII) is involved in the development of atherosclerosis. However, the role of autocrine UII in the development of atherosclerosis remains unclear. Here, we tested the hypothesis that autocrine UII would promote atherosclerosis. Transgenic rabbits were created as a model to study macrophage-specific expressing human UII (hUII) and used to investigate the role of autocrine UII in the development of atherosclerosis. Transgenic rabbits and their nontransgenic littermates were fed a high cholesterol diet to induce atherosclerosis. Comparing the transgenic rabbits with their nontransgenic littermates, it was observed that hUII expression increased the macrophage-positive area in the atherosclerotic lesions by 45% and the positive area ratio by 56% in the transgenic rabbits. Autocrine hUII significantly decreased the smooth muscle cell-positive area ratio in transgenic rabbits (by 54%), without affecting the plasma levels of total cholesterol, triglycerides, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, and glucose and adipose tissue contents. These results elucidated for the first time that autocrine UII plays an important role in the development of atherosclerosis by increasing the accumulation of macrophage-derived foam cell. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
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