1. Could inducible protein-10 and heparin-binding hemagglutinin improve the detection of Mycobacterium tuberculosis-infected subjects in a country with low incidence of tuberculosis?
- Author
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Molicotti, Paola, Bua, Alessandra, Cubeddu, Marina, Ruggeri, Melania, Mura, Maria Stella, Pirina, Pietro, and Zanetti, Stefania
- Subjects
MYCOBACTERIUM tuberculosis ,HEPARIN ,CARRIER proteins ,HEMAGGLUTININ ,DISEASE incidence ,BIOMARKERS ,INTERFERON gamma release tests ,DIAGNOSIS ,DIAGNOSTIC errors ,TUBERCULIN test ,TUBERCULOSIS ,CONTACT tracing ,ODDS ratio - Abstract
Background: This study aimed to evaluate inducible protein-10 (IP-10) as a biomarker besides interferon-gamma (IFN-?) to improve the identification of active tuberculosis (TB) and latent tubercular infection (LTBI) in a country with a low incidence of TB. Methods: Whole blood from Mycobacterium tuberculosis-infected subjects was stimulated with region-of-difference-1 (RD1)-specific peptides and with heparin-binding hemagglutinin (HBHA) to determine the release of IP-10 and IFN-?. Results: No statistically significant difference was observed between positive rates of IP-10 and IFN-? after RD1-specific peptide stimulation in the TB and LTBI groups; a different response was detected in QuantiFERON TB-gold test-negative (QFT-) subjects. A significantly different proportion of positive responses was observed between IP-10 and IFN-? following HBHA stimulation in the TB group and in the QFT- group but not in the LTBI group. Conclusions: The IP-10 test seemed to identify false-negative QFT results in some subjects with a positive IFN-?/IP-10/HBHA pattern. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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