1. Low expression of miR-142-3p promotes intervertebral disk degeneration.
- Author
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Xue J, Hu B, Xing W, Li F, Huang Z, Zheng W, Wang B, Zhu Y, and Yang X
- Subjects
- ADAMTS5 Protein genetics, ADAMTS5 Protein metabolism, Apoptosis genetics, Autophagy genetics, Cell Movement genetics, Cell Survival genetics, Cells, Cultured, Down-Regulation genetics, Humans, Matrix Metalloproteinase 13 genetics, Matrix Metalloproteinase 13 metabolism, MicroRNAs metabolism, Gene Expression, Genetic Association Studies, Intervertebral Disc Degeneration genetics, MicroRNAs genetics, MicroRNAs physiology
- Abstract
Background: Intervertebral disk degeneration (IDD) is a degenerative disease characterized by cytoplasm loss and extracellular matrix degradation. Numerous evidence reported that miRNAs participated in IDD development. Nevertheless, the function of miR-142-3p in IDD development remains unknown. This study mainly explored the potential role and function of miR-142-3p in IDD development., Methods: One percent fetal bovine serum was used to induce the degeneration of ATDC5 cells, and miR-142-3p level was examined by qRT-PCR. Then, miR-142-3p mimic/inhibitor and its corresponding negative control were transfected into ATDC5 normal and degenerative cells. Viability, migration, invasion, apoptosis, cycle, Bax, Bcl-2, P62, and Beclin1 expression levels were assessed using CCK8, wound healing assay, annexin V-FITC/PI staining, western blot, and qRT-PCR, respectively., Results: The results revealed that the expression levels of MMP13, ADAMTS5, MMP3, and Col-X were increased as well as the expression levels of SOX-9 and Col-II were reduced in ATDC5 degenerative cells, indicating the degeneration model was constructed. We observed that miR-142-3p was decreased in ATDC5 degenerative cells and its suppression could promote ATDC5 cell degeneration. However, miR-142-3p overexpression could reverse the cell viability inhibition, as well as apoptosis and autophagy enhancement in ATDC5 degenerative cells., Conclusions: Our results proved that miR-142-3p may play an important role in disk degeneration. Further animal study is needed to illustrate the role of the miR-142-3p in IDD development.
- Published
- 2021
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