1. Two cyanoethylene-based fluorescence probes for highly efficient cyanide detection and practical applications in drinking water and living cells.
- Author
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Peng, Ting, Li, Shining, Zhou, Yuping, Liu, Ruiyuan, and Qu, Jinqing
- Subjects
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CYANIDES , *STOKES shift , *FLUORESCENCE , *DRINKING water , *ACRYLONITRILE , *FLUORESCENCE spectroscopy - Abstract
Cyanide detection methods are urgently needed due to the highly lethal to human beings. Herein, we report two fluorescence probes (Probe 1 and Probe 2) based on cyanoethylene group for cyanide anion (CN−) detection. The selective recognition for CN− was confirmed by the completely opposite green fluorescence of Probe 1 and red fluorescence of Probe 2 observed by fluorescence spectra and naked eyes. The probes take advantages of the large Stokes shift (~160 nm), rapid response (30 s), anti-interference performance and low detection limit (Probe 1 : 12.4 nM, Probe 2 : 101 nM). The sensing mechanism is certificated to the nucleophilic attack of CN− to electron-deficient cyanoethylene group of probes, which was demonstrated by 1H NMR titration, HR-MS, Job's plot and IR spectroscopy. Density functional theory (DFT) calculations were carried out to analyze the mechanism in theory. Further, practical applications were studied. Easy-to-use test strips treated with Probe 1 or Probe 2 are capable of CN− detection in pure drinking water. The good biocompatibility and membrane penetrability have achieved the bioimaging capability of Probe 1 and Probe 2 in living HepG-2 cells, making the probes promising for use in real lives. [Display omitted] • Probes show high selectivity towards cyanide (CN−) and good anti-interference ability against other competing anions. • Probes show high reactivity in aqueous media, making them can be used in actual environment and biological system. • Probes are capable of CN− detection by easy-to-use test in pure drinking water. • Probes have large Stokes shifts and low cytotoxicity so that they can be utilized as CN− sensors in living cells. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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