126 results on '"Feng, Haichao"'
Search Results
2. Temperature field analysis and equal-amplitude temperature rise constraint current control method under open-circuit fault-tolerant operation for five-phase permanent magnet motor
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Jiang, Siyuan, Gao, Mengzhen, Niu, Junheng, Feng, Haichao, Ai, Liwang, and Xu, Xiaozhuo
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- 2024
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3. Analysis and multi-objective optimization design of sinusoidal DSEM with two different excitation modes based on Gray-Fuzzy-Taguchi method
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Jiang, Siyuan, Gao, Mengzhen, Wang, Jiyong, Liu, Junzhe, Feng, Haichao, and Xu, Xiaozhuo
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- 2024
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4. Plant commensal type VII secretion system causes iron leakage from roots to promote colonization
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Liu, Yunpeng, Shu, Xia, Chen, Lin, Zhang, Huihui, Feng, Haichao, Sun, Xiting, Xiong, Qin, Li, Guangqi, Xun, Weibing, Xu, Zhihui, Zhang, Nan, Pieterse, Corné M. J., Shen, Qirong, and Zhang, Ruifu
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- 2023
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5. Mover structure optimization and performance improvement for halbach consequent-pole PM synchronous linear motors with flux barrier
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Xu, Xiaozhuo, Jia, Liumeng, Jiang, Siyuan, and Feng, Haichao
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- 2024
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6. Chemical communication in plant–microbe beneficial interactions: a toolbox for precise management of beneficial microbes
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Xu, Zhihui, Liu, Yunpeng, Zhang, Nan, Xun, Weibing, Feng, Haichao, Miao, Youzhi, Shao, Jiahui, Shen, Qirong, and Zhang, Ruifu
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- 2023
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7. RIN enhances plant disease resistance via root exudate-mediated assembly of disease-suppressive rhizosphere microbiota
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Yang, Keming, Fu, Ruixin, Feng, Haichao, Jiang, Gaofei, Finkel, Omri, Sun, Tianyu, Liu, Mingchun, Huang, Baowen, Li, Shan, Wang, Xiaofang, Yang, Tianjie, Wang, Yikui, Wang, Shimei, Xu, Yangchun, Shen, Qirong, Friman, Ville-Petri, Jousset, Alexandre, and Wei, Zhong
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- 2023
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8. Potassium phosphite enhanced the suppressive capacity of the soil microbiome against the tomato pathogen Ralstonia solanacearum
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Su, Lv, Feng, Haichao, Mo, Xingxia, Sun, Juan, Qiu, Pengfei, Liu, Yunpeng, Zhang, Ruifu, Kuramae, Eiko E., Shen, Biao, and Shen, Qirong
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- 2022
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9. Rhizosphere microbes enhance plant salt tolerance: Toward crop production in saline soil
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Liu, Yunpeng, Xun, Weibing, Chen, Lin, Xu, Zhihui, Zhang, Nan, Feng, Haichao, Zhang, Qiang, and Zhang, Ruifu
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- 2022
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10. Extracellular proteins of Trichoderma guizhouense elicit an immune response in maize ( Zea mays ) plants
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Xu, Yu, Zhang, Jian, Shao, Jiahui, Feng, Haichao, Zhang, Ruifu, and Shen, Qirong
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- 2020
11. Optimization Design of Thrust Characteristics of Switched Flux Linear Motor.
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Feng, Haichao, Yang, Dongqing, Xu, Xiaozhuo, Gao, Caixia, and Ai, Liwang
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PERMANENT magnet motors , *MAGNETIC flux leakage , *SWITCHED reluctance motors , *THRUST , *PERMANENT magnets , *TAGUCHI methods - Abstract
The structure of permanent magnet flux‐switching linear motor (PMFSLM) with permanent magnet and coil as short primary mover and slotted core as long secondary stator can solve the problem of high cost in the long‐stroke transportation field, but PMFSLM has the phenomenon of top magnetic flux leakage. The permanent magnet on the mover side of U‐shaped permanent magnet flux‐switching linear motor (UPM‐FSLM) is arranged in a U shape, which improves the utilization rate of the permanent magnet of the motor. In order to reduce the edge effect and suppress the thrust ripple, based on the size parameters of PMFSLM prototype, a UPM‐FSLM with arc‐shaped assistant permanent magnet teeth structure is proposed, its specific structure and operation mechanism are introduced; In order to obtain the optimal thrust characteristics, the Taguchi method is used to screen the arc‐shaped assistant permanent magnets teeth sensitive parameters, and then the NSGA II algorithm based on the response surface method is used for comprehensive multi‐objective optimization. The results show that compared with PMFSLM, the average thrust of the optimized motor is increased by 16.09%, and the thrust ripple is reduced by 91.78%. © 2023 Institute of Electrical Engineer of Japan and Wiley Periodicals LLC. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Induced root-secreted d-galactose functions as a chemoattractant and enhances the biofilm formation of Bacillus velezensis SQR9 in an McpA-dependent manner
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Liu, Yunpeng, Feng, Haichao, Fu, Ruixin, Zhang, Nan, Du, Wenbin, Shen, Qirong, and Zhang, Ruifu
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- 2020
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13. Listening to plant's Esperanto via root exudates: reprogramming the functional expression of plant growth‐promoting rhizobacteria.
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Feng, Haichao, Fu, Ruixin, Luo, Jiayu, Hou, Xueqin, Gao, Kun, Su, Lv, Xu, Yu, Miao, Youzhi, Liu, Yunpeng, Xu, Zhihui, Zhang, Nan, Shen, Qirong, Xun, Weibing, and Zhang, Ruifu
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PLANT growth-promoting rhizobacteria , *PLANT exudates , *GENE expression , *PLANT growth , *PLANT succession , *PLANT growth promoting substances , *SOIL microbiology - Abstract
Summary: Rhizomicrobiome plays important roles in plant growth and health, contributing to the sustainable development of agriculture. Plants recruit and assemble the rhizomicrobiome to satisfy their functional requirements, which is widely recognized as the 'cry for help' theory, but the intrinsic mechanisms are still limited.In this study, we revealed a novel mechanism by which plants reprogram the functional expression of inhabited rhizobacteria, in addition to the de novo recruitment of soil microbes, to satisfy different functional requirements as plants grow. This might be an efficient and low‐cost strategy and a substantial extension to the rhizomicrobiome recruitment theory.We found that the plant regulated the sequential expression of genes related to biocontrol and plant growth promotion in two well‐studied rhizobacteria Bacillus velezensis SQR9 and Pseudomonas protegens CHA0 through root exudate succession across the plant developmental stages. Sixteen key chemicals in root exudates were identified to significantly regulate the rhizobacterial functional gene expression by high‐throughput qPCR.This study not only deepens our understanding of the interaction between the plant–rhizosphere microbiome, but also provides a novel strategy to regulate and balance the different functional expression of the rhizomicrobiome to improve plant health and growth. [ABSTRACT FROM AUTHOR]
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- 2023
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14. Novel fatty acids-governed cannibalism in beneficial rhizosphere Bacillus enhances biofilm formation via a two-component system OmpS/R and toxin transporter
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Huang, Rong, Li, Qing, Wang, Dandan, Feng, Haichao, Zhang, Nan, Shao, Jiahui, Shen, Qirong, Xu, Zhihui, and Zhang, Ruifu
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- 2023
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15. Fault detection and classification for complex processes using semi-supervised learning algorithm
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Wang, Xiaogang, Feng, Haichao, and Fan, Yunpeng
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- 2015
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16. pheS * , an effective host-genotype-independent counter-selectable marker for marker-free chromosome deletion in Bacillus amyloliquefaciens
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Zhou, Chaoyang, Shi, Lingling, Ye, Bin, Feng, Haichao, Zhang, Ji, Zhang, Ruifu, and Yan, Xin
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- 2017
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17. Sustained Inhibition of Maize Seed‐Borne Fusarium Using a Bacillus‐Dominated Rhizospheric Stable Core Microbiota with Unique Cooperative Patterns.
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Xun, Weibing, Ren, Yi, Yan, He, Ma, Aiyuan, Liu, Zihao, Wang, Lingling, Zhang, Nan, Xu, Zhihui, Miao, Youzhi, Feng, Haichao, Shen, Qirong, and Zhang, Ruifu
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FUSARIUM ,SUSTAINABLE agriculture ,RHIZOSPHERE ,FUNCTIONAL status ,RHIZOBACTERIA - Abstract
Seed‐borne pathogens can inhabit the rhizosphere and infect the plant after germination. The rhizosphere microbiome plays critical roles in defending against seed‐borne pathogens. However, the assembly of a core rhizosphere microbiome to suppress seed‐borne pathogens is unknown. Here, the root‐associated microbiome is infested with seed‐borne Fusarium in sterile environment, while the root‐associated microbiome is not infested when it interacts with the native soil microbiome across maize cultivars, suggesting that a core rhizosphere microbiome assembles to suppress seed‐borne Fusarium. Two strategies of progressive dilution and rhizodepositional attraction are applied to identify the core rhizobacteria. A synthetic microbiota (SynM) is constructed using the isolates of the core rhizobacteria and optimized according to superior community stability and Fusarium‐suppression capability, which surpasses the single strain and randomly formed microbiota. The optimized SynM (OptSynM) presents a distinctive cooperative pattern in which a key strain harbors the Fusarium suppression function by synthesizing the antagonistic substance fengycin, while other members intensify the functional performance by promoting the growth and the expression of the antagonistic and plant‐growth‐promoting related genes of the key strain. This study demonstrates innovative approaches to construct stable and minimal microbiota for sustainable agriculture and proposes a unique cooperative pattern to sustain community stability and functionality. [ABSTRACT FROM AUTHOR]
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- 2023
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18. A novel online method for locating fault coil in direct‐drive permanent magnet synchronous motor with inter‐turn short‐circuit fault using search coil array.
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Chen, Hao, Zhang, Nan, Gao, Caixia, Xu, Xiaozhuo, Feng, Haichao, Si, Jikai, and Hu, Yihua
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FAULT location (Engineering) ,ELECTROMOTIVE force ,SIGNAL processing ,PERMANENT magnet motors ,ELECTRIC fault location - Abstract
This study presents a fault coil localisation approach for a direct‐drive permanent magnet synchronous motor (DDPMSM) with inter‐turn short‐circuit fault (ISF) using the search coil (SC) array. The SCs are wound around specific stator tooth of DDPMSM through the proposed SC array arrangement principle, which reduces the invasiveness of the fault coil localisation method. The SCs are classified as several search coil groups (SCGs), the differential between SC back electromotive forces (EMFs) in the same SCGs (δ1) and SC residual back EMF (δ2) are used as fault indicators for ISF localisation. The peak values extracted from δ1 and δ2 are the basis for diagnosing ISF. Hence, the proposed approach does not need a complicated signal processing algorithm, and it is less complex. First, the SC back EMF analytical model is developed to investigate the mapping relationship between the back EMF of each SC and fault coil location. Second, fault coil localisation indicators for ISF are analysed based on the proposed analytical model, and the exact ISF localisation approach is presented. Finally, two basic installation units and the arrangement principle of SC array in DDPMSM are proposed. Simulation and experimental results justify the effectiveness of the selected fault indicators and the feasibility of the proposed SC array for diagnosing ISF. [ABSTRACT FROM AUTHOR]
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- 2023
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19. Multi‐objective optimisation design of a suspension‐guided permanent magnet synchronous linear motor for ropeless elevator.
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Xu, Xiaozhuo, Zhang, Yangyang, Feng, Haichao, Zhao, Yunji, and Zhou, Menglin
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SYNCHRONOUS electric motors ,PERMANENT magnets ,MAGNETIC levitation vehicles ,ELEVATORS ,FINITE element method ,MATHEMATICAL optimization - Abstract
In this article, a suspension‐guided permanent magnet synchronous linear motor (SG‐PMSLM) for ropeless elevator is proposed, which can meet the requirements of high thrust and high thrust density for ropeless elevator, and reduce vibration noise and wheel‐rail wear during motor operation. The structure, working principle and electromagnetic characteristics of SG‐PMSLM are analysed. In order to improve the overall performance of SG‐PMSLM, this article aims at improving the average thrust and thrust density, reducing the thrust fluctuation of SG‐PMSLM. Firstly, the sensitivity analysis of the key structural parameters of SG‐PMSLM is conducted, and the parameters that have significant influence on the three optimisation objectives are selected as the optimisation variables. Secondly, the response surface model of three optimisation objectives is built, and the Particle Swarm Optimisation algorithm is combined to conduct the multi‐objective optimisation design for SG‐PMSLM. Thirdly, the optimised solution set is obtained for three objectives. Finite Element Analysis is used to verify that the optimised solution set has better diversity and can effectively improve SG‐PMSLM overall performance compared with the initial design. Finally, the electromagnetic performance of SG‐PMSLM is verified by experiments. [ABSTRACT FROM AUTHOR]
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- 2023
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20. Performance analysis and optimization of permanent magnet flux switched linear motor for ropeless elevator.
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Xu, Xiaozhuo, Zhang, Wei, Feng, Haichao, Zhao, Yunji, and Ai, Liwang
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PERMANENT magnets ,ELEVATORS ,ELECTROMAGNETS ,SYNCHRONOUS electric motors ,THRUST - Abstract
Permanent magnet linear synchronous motor (PMLSM) is one of the ideal driving sources of ropeless elevators. However, such motors, whether moving magnetic or moving coil, will produce the problem of excessive cost under long travel. The permanent magnet flux switched linear motor (PMFSLM) with permanent magnets (PMs) and copper coils on the mover side greatly reduces the cost. A U‐type PMFSLM is proposed in the paper. First, a PMFSLM is proposed and investigated, quantitatively comparing with PMLSM for the ropeless elevator system. Second, aiming at the problem of flux leakage at the top of PMFSLM, a U‐type PM (UPM) structure is presented. The electromagnetic performance of PMFSLM and UPM‐FSLM have been compared and summarised. The comparison results show that UPM‐FSLM solves the defect of top flux leakage, increases thrust by 4.34%, reduces thrust ripple by 28.10%, and has better electromagnetic performance than PMFSLM. Afterwards, the elevator passenger capacity of PMFSLM and UPM‐FSLM are simulated and calculated and the feasibility is analysed. Finally, a prototype of PMFSLM has been built and measured. [ABSTRACT FROM AUTHOR]
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- 2023
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21. Research on Carbon Emission Characteristics of Rural Buildings Based on LMDI-LEAP Model.
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Feng, Haichao, Wang, Ruonan, and Zhang, He
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CARBON emissions , *ENERGY consumption of buildings , *COMMERCIAL building energy consumption , *ENERGY intensity (Economics) , *ENERGY conservation , *ELECTRIC power consumption - Abstract
Based on the emission factor method and LMDI-LEAP model, this paper systematically studies the current situation, influencing factors and changing trend of carbon emissions from rural buildings in a typical village located in southern China. The results showed that (1) the per capita carbon emissions generated by the energy consumption of rural buildings is 2.58 tCO2/a. Carbon emissions from electricity consumption in buildings account for about 96.07%; (2) the per capita building area, building area energy intensity, population size, population structure and carbon emission coefficient affect rural building carbon emissions, with contribution rates of 70.13%, 31.27%, 0.61%, −1.21% and −0.80%, respectively; (3) from 2021 to 2060, the carbon emissions of rural buildings are expected to increase first and then decrease. In 2021, the base year, carbon emissions from buildings were 2755.49 tCO2. The carbon emissions will peak at 5275.5 tCO2. Measures such as controlling the scale of buildings and improving the utilization rate of clean energy can effectively reduce carbon emissions, in which case the peak can be reduced to 4830.06 tCO2. Finally, the countermeasures and suggestions about rural building energy saving and emission reduction are proposed, including improving the construction management, raising energy efficiency standards in buildings, increasing the proportion of clean energy and raising residents' awareness of energy conservation. [ABSTRACT FROM AUTHOR]
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- 2022
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22. Levitation behaviours of an eccentric SC-PM system with multi-degree-of-freedom motion.
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Ai, Liwang, Miao, Sen, Li, Na, Gao, Caixia, Feng, Haichao, and Xu, Xiaozhuo
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HIGH temperature superconductors ,LEVITATION ,MAGNETIC suspension ,FINITE element method - Abstract
The high temperature superconducting (HTS) magnetic levitation is actually a self-stable levitation with multi-degree-of-freedom (DoF). However, the research of HTS magnetic levitation focuses on the axial or radial levitation behaviour with single-DoF motion independently. This article mainly discusses the 3-D modelling and the levitation behaviours of an eccentric superconductor-permanent magnet (SC-PM) system with multi-DoF motion. Firstly, the Power-Law E − J relation is used to obtain the relationship of equivalent conductivity-electric field strength for the superconductor. Then, a 3-D finite element model (FEM) of SC-PM system is established and calculated in software MagNet. At the same time, the result of the simulation is verified by the experimental measurements. The axial levitation behaviour is analysed by establishing and solving the 3-D FEM in eccentric SC-PM system with different multi-DoF motions. The results show that the maximum axial levitation force decreases with the increase of the static eccentricity distance. When the PM rotor exhibits dynamic eccentricity, the axial levitation force presents periodic fluctuations of which the amplitude and period are related to the dynamic eccentric distance and speed. [ABSTRACT FROM AUTHOR]
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- 2022
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23. Torque ripple reduction of brushless DC motor with convex arc‐type permanent magnets based on robust optimization design.
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Feng, Haichao, Zhang, Sheng, Wei, Jinsong, Xu, Xiaozhuo, Gao, Caixia, and Ai, Liwang
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ROBUST optimization , *PERMANENT magnets , *AIR gap flux , *BRUSHLESS electric motors , *MAGNETIC pole , *MAGNETIC structure - Abstract
The mass production of the motors causes tolerance of shape and dimension, deviation of permanent magnet remanence and rotor eccentricity error, which affect the cogging torque and torque ripple amplitude and performance consistency. In order to reduce the torque ripple of the motor in the actual condition, the robustness optimization is performed in the paper. Firstly, an improving convex arc type permanent magnet structure is adopted to improve air gap flux density and suppress the cogging torque. Secondly, the structure parameters of the magnetic pole are selected as optimization variables, and the magnetization angle, remanence and position of the permanent magnet, static and dynamic rotor eccentricity are considered as noise factors. To improve the overall robustness of the motor under different operating conditions, the dynamic Taguchi method is used to optimize the robustness of the motor, and the test data is processed through the relation analysis method to obtain the optimal combination of control factors. Finally, the prototype is manufactured for the experiment. Compared with the single‐operating conditions robust optimization results, the robust optimization method improves the robustness of the motor. The cogging torque amplitude is reduced by 39.2%. The torque ripple is 38.4% lower than that before optimization. [ABSTRACT FROM AUTHOR]
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- 2022
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24. Analysis and testing of a superconducting maglev submersible cryogenic liquid pump.
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Ai, Liwang, Zhang, Guomin, Xu, Xiaozhuo, Feng, Haichao, and Cao, Wenping
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SUPERCONDUCTING magnets ,PERMANENT magnets ,ELECTROMAGNETS ,SUPERCONDUCTORS ,LIQUID nitrogen - Abstract
This paper studies a superconducting maglev submersible cryogenic liquid pump (CLP) using superconducting magnetic bearing (SMB). A new structure is proposed for the self‐stability of high‐temperature superconducting magnetic levitation, and the compact structure of disk motor‐pump. The rotor of the axial‐flux disk motor is connected with a centrifugal impeller to form an impeller‐rotor of the proposed superconducting CLP. Supported by a SMB system, the impeller‐rotor rotates frictionlessly with self‐stabilisation as a suspension rotor. The SMB system includes a radial‐type SMB, an auxiliary permanent magnet bearing, and an electromagnetic bearing composed of the stator and the rotor of disk motor. Theoretical calculations and experimental measurements are performed to examine the axial and radial levitation force behaviours of the SMB system. Then, a kinetic model of the suspension rotor is established and analysed to assure force balance and stable levitation. Finally, a prototype pump is fabricated and an experimental setup for liquid nitrogen transmission is constructed for validation purposes. The experimental tests on the pump head and the flow rate are carried out, which demonstrate the effectiveness of the proposed superconducting maglev submersible CLP. [ABSTRACT FROM AUTHOR]
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- 2022
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25. The volatile cedrene from Trichoderma guizhouense modulates Arabidopsis root development through auxin transport and signalling.
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Li, Yucong, Shao, Jiahui, Fu, Yansong, Chen, Yu, Wang, Hongzhe, Xu, Zhihui, Feng, Haichao, Xun, Weibing, Liu, Yunpeng, Zhang, Nan, Shen, Qirong, Xuan, Wei, and Zhang, Ruifu
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ROOT development ,AUXIN ,PLANT roots ,ROOT formation ,TRICHODERMA ,ROOT growth - Abstract
Rhizosphere microorganisms interact with plant roots by producing chemical signals that regulate root development. However, the distinct bioactive compounds and signal transduction pathways remain to be identified. Here, we showed that sesquiterpenes are the main volatile compounds produced by plant‐beneficial Trichoderma guizhouense NJAU4742. Inhibition of sesquiterpene biosynthesis eliminated the promoting effect of this strain on root growth, indicating its involvement in plant‐fungus cross‐kingdom signalling. Sesquiterpene component analysis identified cedrene, a highly abundant sesquiterpene in strain NJAU4742, to stimulate plant growth and root development. Genetic analysis and auxin transport inhibition showed that the TIR1 and AFB2 auxin receptors, IAA14 auxin‐responsive protein, and ARF7 and ARF19 transcription factors affected the response of lateral roots to cedrene. Moreover, the AUX1 auxin influx carrier and PIN2 efflux carrier were also found to be indispensable for cedrene‐induced lateral root formation. Confocal imaging showed that cedrene affected the expression of pPIN2:PIN2:GFP and pPIN3:PIN3:GFP, which might be related to the effect of cedrene on root morphology. These results suggested that a novel sesquiterpene molecule from plant‐beneficial T. guizhouense regulates plant root development through the transport and signalling of auxin. Summary Statement: From the volatile compounds produced by a plant beneficial fungus, we identified a novel component cedrene that can promote plant root development. We revealed the role of auxin signalling and transport in the process of cedrene‐promoted root development. This study complements the lack of identification of distinct bioactive compounds from microbial sources and provides a helpful tool to study and decipher the underlying cellular and molecular reactions and mechanism occurring in microbe‐plant interactions. [ABSTRACT FROM AUTHOR]
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- 2022
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26. Feasibility analysis and optimization design of PMSM with 120° phase belts toroidal windings for electric vehicles.
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Si, Jikai, Gao, Mengzhen, Yang, Xiangyu, Gao, Caixia, Feng, Haichao, and Hu, Yihua
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TOROIDAL magnetic circuits ,SYNCHRONOUS electric motors ,RELUCTANCE motors ,ELECTROMAGNETIC actuators ,ELECTRIC motors - Abstract
A permanent magnet synchronous motor (PMSM) is introduced with 120° phase belts toroidal windings (PBTWs) (120°PBTWPMSM), in which its armature reaction field principle is elaborated. Then, the experiment concerning the 120°PBTWPMSM is conducted to verify the validity of the related analysis and the feasibility of the 120°PBTWPMSM. In addition, a method of shifting the magnetic poles (SMP) is adopted to suppress the cogging torque and the torque ripple. After this, the 120°PBTWPMSM after SMP is further optimised by the Taguchi method to obtain the structural parameters of the motor with optimal comprehensive performance. Finally, the performance of the original motor, the 120°PBTWPMSM after SMP and the optimal motor are compared, which verify the effectiveness of the optimization methods. [ABSTRACT FROM AUTHOR]
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- 2021
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27. Bacillus velezensis tolerance to the induced oxidative stress in root colonization contributed by the two‐component regulatory system sensor ResE.
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Zhang, Huihui, Liu, Yunpeng, Wu, Gengwei, Dong, Xiaoyan, Xiong, Qin, Chen, Lin, Xu, Zhihui, Feng, Haichao, and Zhang, Ruifu
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PLANT colonization ,OXIDATIVE stress ,PLANT growth-promoting rhizobacteria ,REACTIVE oxygen species ,DISEASE resistance of plants ,CUCUMBERS ,MUTAGENESIS - Abstract
Efficient root colonization of plant growth‐promoting rhizobacteria is critical for their plant‐beneficial functions. However, the strategy to overcome plant immunity during root colonization is not well understood. In particular, how Bacillus strains cope with plant‐derived reactive oxygen species (ROS), which function as the first barrier of plant defence, is not clear. In the present study, we found that the homolog of flg22 in Bacillus velezensis SQR9 (flg22SQR9) has 78.95% identity to the typical flg22 (flg22P.s.) and induces a significant oxidative burst in cucumber and Arabidopsis. In contrast to pathogenic or beneficial Pseudomonas, live B. velezensis SQR9 also induced an oxidative burst in cucumber. We further found that B. velezensis SQR9 tolerated higher H2O2 levels than Pst DC3000, the pathogen that harbours the typical flg22, and that it possesses the ability to suppress the flg22‐induced oxidative burst, indicating that B. velezensis SQR9 may exploit a more efficient ROS tolerance system than DC3000. Further experimentation with mutagenesis of bacteria and Arabidopsis showed that the two‐component regulatory system, ResDE, in B. velezensis SQR9 is involved in tolerance to plant‐derived oxidative stress, thus contributing to root colonization. This study supports a further investigation of the interaction between beneficial rhizobacteria and plant immunity. The beneficial rhizobacterium Bacillus velezensis SQR9 or its flg22 peptides triggered oxidative burst in cucumber and Arabidopsis. The two‐component regulatory system, ResDE, in B. velezensis SQR9 is involved in tolerance to plant‐derived oxidative stress, thus contributing to root colonization. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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28. Participating mechanism of a major contributing gene ysnE for auxin biosynthesis in Bacillus amyloliquefaciens SQR9.
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Shao, Jiahui, Li, Yucong, Li, Zunfeng, Xu, Zhihui, Xun, Weibing, Zhang, Nan, Feng, Haichao, Miao, Youzhi, Shen, Qirong, and Zhang, Ruifu
- Subjects
BACILLUS amyloliquefaciens ,PLANT growth-promoting rhizobacteria ,PLANT growth ,BIOSYNTHESIS ,AUXIN - Abstract
The phytohormone indole‐3‐acetic acid (IAA) has been demonstrated to contribute to the plant growth‐promoting effect of rhizobacteria, but the IAA biosynthesis pathway in rhizobacteria remains unclear. The ysnE gene, encoding a putative tryptophan acetyltransferase, has been demonstrated to be involved in and strongly contribute to IAA production in Bacillus, but the mechanism is unknown. In this study, to investigate how ysnE participates in IAA biosynthesis in the plant growth‐promoting rhizobacterium Bacillus amyloliquefaciens SQR9, differences in the produced IAA biosynthesis intermediates between wild‐type SQR9 and ΔysnE were analyzed and compared, and the effects of different intermediate compounds on the production of IAA and the accumulation of other intermediates were also investigated. The results showed that the mutant ΔysnE produced more indole‐3‐lactic acid (ILA) and tryptamine (TAM) than the SQR9 wild‐type strain (nearly 1.6‐ and 2.1‐fold), while the production of tryptophol (TOL) was significantly decreased by 46%. When indole‐3‐pyruvic acid (IPA) served as the substrate, the concentration of ILA in the ΔysnE fermentation broth was much higher than that of the wild type, while IAA and TOL were significantly lower, and ΔysnE was lower than SQR9 in IAA and TOL with the addition of TAM. The TOL content in the ΔysnE fermentation broth was much lower than that in the wild‐type SQR9 with the addition of ILA. We suggest that ysnE may be involved in the IPA and TAM pathways and play roles in indole acetaldehyde (IAAld) synthesis from IPA and TAM and in the conversion of ILA to TOL. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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29. Overcoming diverse homologous recombinations and single chimeric guide RNA competitive inhibition enhances Cas9‐based cyclical multiple genes coediting in filamentous fungi.
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Miao, Youzhi, Xia, Yanwei, Kong, Yanqiong, Zhu, Han, Mei, Huiling, Li, Pan, Feng, Haichao, Xun, Weibing, Xu, Zhihui, Zhang, Nan, Liu, Dongyang, Shen, Qirong, and Zhang, Ruifu
- Subjects
FILAMENTOUS fungi ,GENES ,GENOME editing ,FUNGAL genetics ,RNA ,DNA - Abstract
Summary: Deciphering the complex cellular behaviours and advancing the biotechnology applications of filamentous fungi increase the requirement for genetically manipulating a large number of target genes. The current strategies cannot cyclically coedit multiple genes simultaneously. In this study, we firstly revealed the existence of diverse homologous recombination (HR) types in marker‐free editing of filamentous fungi, and then, demonstrated that sgRNA efficiency‐mediated competitive inhibition resulted in the low integration of multiple genetic sites during coediting, which are the two major obstacles to limit the efficiency of cyclically coediting of multiple genes. To overcome these obstacles, we developed a biased cutting strategy by Cas9 to greatly enhance the desired HR type and applied a new selection marker labelling strategy for multiple donor DNAs, in which only the donor DNA with the lowest sgRNA efficiency was labelled. Combined with these strategies, we successfully developed a convenient method for cyclically coediting multiple genes in different filamentous fungi. In addition, diverse HRs resulted in a useful and convenient one‐step approach for gene functional study combining both gene disruption and complementation. This research provided both a useful one‐step approach for gene functional study and an efficient strategy for cyclically coediting multiple genes in filamentous fungi. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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30. Volatile compounds from beneficial rhizobacteria Bacillus spp. promote periodic lateral root development in Arabidopsis.
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Li, Yucong, Shao, Jiahui, Xie, Yuanming, Jia, Letian, Fu, Yansong, Xu, Zhihui, Zhang, Nan, Feng, Haichao, Xun, Weibing, Liu, Yunpeng, Shen, Qirong, Xuan, Wei, and Zhang, Ruifu
- Subjects
ROOT formation ,PLANT growth-promoting rhizobacteria ,RHIZOBACTERIA ,BACILLUS amyloliquefaciens ,FREQUENCIES of oscillating systems ,ARABIDOPSIS ,PLANT growth - Abstract
Lateral root formation is coordinated by both endogenous and external factors. As biotic factors, plant growth‐promoting rhizobacteria can affect lateral root formation, while the regulation mechanism is unclear. In this study, by applying various marker lines, we found that volatile compounds (VCs) from Bacillus amyloliquefaciens SQR9 induced higher frequency of DR5 oscillation and prebranch site formation, accelerated the development and emergence of the lateral root primordia and thus promoted lateral root development in Arabidopsis. We demonstrated a critical role of auxin on B. amyloliquefaciens VCs‐induced lateral root formation via respective mutants and pharmacological experiments. Our results showed that auxin biosynthesis, polar transport and signalling pathway are involved in B. amyloliquefaciens VCs‐induced lateral roots formation. We further showed that acetoin, a major component of B. amyloliquefaciens VCs, is less active in promoting root development compared to VC blends from B. amyloliquefaciens, indicating the presence of yet uncharacterized/unknown VCs might contribute to B. amyloliquefaciens effect on lateral root formation. In summary, our study revealed an auxin‐dependent mechanism of B. amyloliquefaciens VCs in regulating lateral root branching in a non‐contact manner, and further efforts will explore useful VCs to promote plant root development. The volatile compounds from Bacillus spp. increase the frequency of prebranch site formation and further accelerated the emergence of lateral root primordia (LRP) from the primary root (PR) in Arabidopsis, resulting in a denser lateral root (LR) along the PR spatially. This process is dependent on auxin signaling pathway; fully functional machinery of YUCs‐mediated auxin biosynthesis and polar auxin transport are also required. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
31. Fault coil location of inter‐turn short‐circuit for direct‐drive permanent magnet synchronous motor using knowledge graph.
- Author
-
Lv, Ke, Gao, Caixia, Si, Jikai, Feng, Haichao, and Cao, Wenping
- Abstract
Inter‐turn short‐circuit fault (ISF) degrades its reliability and may cause serious catastrophes for direct‐drive permanent magnet synchronous motor (DDPMSM). Fault location technology can reduce maintenance time, increase the mean time between failure (MTBF), and then improve the reliability of DDPMSM. Hence, an intelligent fault locating system for DDPMSM is proposed in this paper. This system proposes a knowledge graph (KG) based diagnostic tool for detection and location of the fault coil. First, the fault model of the DDPMSM with multiple branches parallel winding is established, which is used to analyze the fault characteristics of motor. Second, the BDC and BRC are proposed as the fault indicator. The effectiveness and robustness of fault indicator are analyzed. Then, the KG system are designed and established according to the relationship between fault indicator and location of fault coil. Finally, the system is tested by data under different fault and operation conditions. The test results showed that the proposed fault locating system can detect and locate the fault coil in early stage. The minimum ratio of shorted turns to branch turns that can be detected is 0.52%. The minimum ratio of shorted turns to branch turns that can be located is 6.25%. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
32. Novel modelling method based on winding sub‐element of direct‐drive permanent magnet synchronous motor.
- Author
-
Gao, Caixia, Lv, Ke, Si, Jikai, Feng, Haichao, and Gan, Chun
- Abstract
Owing to its many benefits, including convenient and short computing time, the analytical model (AM) is widely used in the performance analyse of the permanent magnet synchronous motor (PMSM). The existing analytical models based on phase winding cannot sufficiently speculate about the contribution of the coil to the performances of direct‐drive PMSM (DDPMSM) with series coils and multiple branches. Hence, in this work, a novel AM for the DDPMSM is proposed. This model, which is based on the winding sub‐element, can analyse the influence of coil on motor performance. First, the structure and key parameters of the DDPMSM are reported. Second, an inductance matrix, which considers the spatial disposition of coils and the characteristics of large self‐inductance and small mutual inductance, is constructed. Then, the influence of the coil on motor performance is analysed based on the proposed AM. Finally, the results of AM, finite‐element model and experiment are compared. The results validate the correctness of the proposed AM. Furthermore, the proposed AM can speculate about the contribution of a coil in the DDPMSM performances with satisfactory accuracy. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
33. Characteristics Analysis of the Motions of the Two-Degree-of-Freedom Direct Drive Induction Motor.
- Author
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Xie, Lujia, Si, Jikai, Hu, Yihua, Feng, Haichao, and Ni, Kai
- Subjects
MOTION analysis ,PERMANENT magnet motors ,RELUCTANCE motors ,MAGNETIC fields ,INDUCTION motors - Abstract
The 2-degree-of-freedom direct drive induction motor (2DoFDDIM) is capable of implementing rotary linear or helical motion, which has the definite advantages of integrated structure, high material utilization, and so on. In this paper, the characteristics analysis of the three kinds of motions is presented. The air-gap magnetic field distributions and mechanical characteristics of the 2DoFDDIM with three motions are derived respectively. The results show that there is super synchronous speed or velocity phenomenon for rotary or linear motion. Moreover, the coupling effects between the rotating and travelling wave magnetic fields and that between the two mechanical motions will deteriorate the performance of the three-dimensional (3-D) helical motion compared with the two-dimensional (2-D) motion (rotary or linear). The expressions of the resistant torque and force caused by the coupling effect are derived, which are verified by 3-D finite-element models (FEMs). The results regarding the weakening of the output torque of helical motion and interacting magnetic field obtained from FEMs are then verified by experimental tests. Finally, the skewed slot method is applied to 2DoFDDIM, which successfully improves the performance of helical motion aiming to compensate the coupling resistant torque and force. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
34. Dynamic magnetic‐coupling effect of two‐degrees‐of‐freedom direct drive induction motor.
- Author
-
Feng, Haichao, Si, Jikai, Wang, Peixin, Hua, Wei, Cheng, Zhiping, and Li, Zhongwen
- Subjects
- *
INDUCTION machinery , *MAGNETIC coupling , *MAGNETIC fields , *FINITE element method , *INDUCTION motors , *PERMANENT magnet generators - Abstract
The special structure of the two‐degrees‐of‐freedom direct drive induction motor (2DoFDDIM) gives rise to the magnetic‐coupling between the rotary and linear magnetic fields. In this article, the dynamic magnetic‐coupling effect, which is against the static coupling effect and indicates the special phenomenon caused by coupling magnetic fields in helical motion, is exposed and investigated based on the 3D finite‐element model (3D FEM). The control variable method is adopted and only the coupling magnetic field in helical motion is considered in this research. The performance changes caused by the dynamic magnetic‐coupling effect of the motor are simulated and analyzed. The relationship between the coupling magnetic field and the dynamic magnetic‐coupling effect is disclosed. To validate the 3D FEM and the magnetic‐coupling effect of 2DoFDDIM, a prototype and an experimental platform were built and measured. For the induced voltages caused by the magnetic‐coupling effect, the finite element analysis results are in agreement with the experimental results. © 2019 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
35. Heat dissipation analysis and multi-objective optimization of Raspberry Pi CPU by natural convection.
- Author
-
Wei, Yin, Yao, Junkai, Feng, Haichao, Wu, Qiaoyun, Li, Yuefeng, Zou, Jun, and Zheng, Hao
- Abstract
AbstractWith the high-speed and high-frequency operation of CPU chips, as well as miniaturization and dense assembly of integrated circuits, the heat generated by the Raspberry Pi CPU is constantly increasing. To address the urgent need for efficient Raspberry Pi CPU heat dissipation and ensure normal operation within a safe temperature range, this study first established a model to simulate the CPU's thermal physics parameters during operation using CFD simulation software. An experimental platform was then set up to validate the CFD simulation results. Based on the validated CFD model, initial heat dissipation design was carried out. On this basis, the heat dissipation system was optimized by altering the heat sink’s length, width, fin height, thickness, spacing, and the thermal grease’s thermal conductivity between the heat sink and CPU chip. A surrogate model was established using the response surface methodology, and the NSGA-II genetic algorithm was employed for multi-objective optimization of the heat dissipation system’s cooling performance. Compared to the initial solution, the chip temperature was reduced by 6.19 °C, but the heat sink mass was increased by 0.65 g. By conducting multi-objective optimization design and making reasonable choices for the parameters, a suitable and an optimum design, in terms of both economics and efficiency, is obtained. These results demonstrate that this research can provide theoretical guidance and technical support for practical production. Furthermore, the methods and findings from this study can be applied to the design of heat dissipation systems for similar applications. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
36. Equivalent Circuit Model of Novel Solid Rotor Induction Motor with Toroidal Winding Applying Composite Multilayer Theory.
- Author
-
Feng, Haichao, Cui, Xu, Si, Jikai, Gao, Caixia, and Hu, Yihua
- Subjects
INDUCTION motors ,FINITE element method ,ROTORS ,WINDS ,MAGNETIC fields - Abstract
In this work, a novel solid rotor induction motor with toroidal winding (N-TWSRIM) is proposed and its structure and main structural parameters are given. The operating principle is analyzed in accordance with the movement of the armature magnetic field at different times. An equivalent circuit model (ECM) of the N-TWSRIM is established based on composite multilayer theory to analyze and calculate motor performance quickly and accurately. Electromagnetic performance, which includes output torque, stator current, and power factor under different slip, is calculated with ECM, and its results are compared with those of the finite element method. A prototype of the N-TWSRIM is built and experimented on to validate the correctness of the operating principle and ECM. Experimental results on stator current and torque are consistent with the finite element and analytical results. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
37. Helical Motion Analysis of the 2-Degree-of-Freedom Split-Stator Induction Motor.
- Author
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Xie, Lujia, Si, Jikai, Hu, Yihua, and Feng, Haichao
- Subjects
STATORS ,INDUCTION machinery ,MOTION analysis ,EQUATIONS of motion ,PERMANENT magnet motors ,RELUCTANCE motors - Abstract
In this paper, the characteristics of the helical motion of the rotatory-linear 2-degree-of-freedom split-stator induction motor (2DoFSSIM) are investigated, which cannot be regarded as the simple superimposition of the 1DoF rotary and linear motions. A set of torque and force equations of the helical motion is deduced. It illustrates that the interacting electromagnetic torque and force are opposite to the directions of rotary and axial movements, respectively. For the rotating movement, the interacting torque is related to the axial velocity. Moreover, the axial velocity significantly influences the output torque performances and no-load speed. Similar conclusions can be obtained for axial movement of the helical motion. The 3-D finite-element method is applied to calculate the exact output performance considering the interacting influence. Furthermore, the prototype machine is manufactured and tested to verify the analytical results. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
38. Improved Fuzzy-Based Taguchi Method for Multi-Objective Optimization of Direct-Drive Permanent Magnet Synchronous Motors.
- Author
-
Guo, Youquan, Si, Jikai, Gao, Caixia, Feng, Haichao, and Gan, Chun
- Subjects
PERMANENT magnet motors ,TAGUCHI methods ,FUZZY numbers ,FUZZY systems ,SIGNAL-to-noise ratio - Abstract
The original fuzzy-based Taguchi method is restricted with the number of objective functions. Aiming to solve this problem, this paper describes an improved fuzzy-based Taguchi method (IFBTM) coupled with finite-element analysis (FEA) for the multi-objective optimization of direct-drive permanent magnet synchronous motors (DDPMSMs). To reduce the number of dimensions in the fuzzy rule base, the IFBTM uses a factor stratification method to establish multilayer fuzzy rule base. The fuzzy inference system with multilayer fuzzy rule base is introduced to translate the multiple optimization objectives to a single objective. After optimal selection analysis, the best combination of motor structure factors is obtained with small FEA computation. Finally, the performances of DDPMSM are analyzed and compared with those of IFBTM. The results certify the superiority and validity of IFBTM. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
39. Equivalent Circuit Parameter Calculations and Characteristics Analysis of 2-DoF Direct Drive Induction Motor with a Slotted Solid Rotor.
- Author
-
Feng, Haichao, Si, Jikai, Wu, Wei, Dong, Lianghui, and Cheng, Zhiping
- Subjects
INDUCTION machinery ,INDUCTION motors ,FINITE element method ,CURRENT distribution ,ROTORS - Abstract
In this paper, a modified equivalent circuit model (ECM), which considers the effects of an arc-shaped stator structure and saturation, is presented to calculate the characteristics of a two-degree-of-freedom direct drive induction motor (2DoFDDIM). The motor has a novel slotted solid rotor (SSR), which is slotted along the axial and circumferential directions, and copper is cast in the slots. The SSR is equivalent to a cage rotor by analyzing the current distribution using the circuit diagram. The corrected ECM parameter expressions of a cage rotor are proposed to calculate the SSR parameters. The characteristics obtained by ECM and finite element method are compared to verify the accuracy of the modified ECM. This paper provides reference for calculating the parameters of the induction motor with SSR. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
40. Recognition of dominant attractants by key chemoreceptors mediates recruitment of plant growth‐promoting rhizobacteria.
- Author
-
Feng, Haichao, Zhang, Nan, Fu, Ruixin, Liu, Yunpeng, Krell, Tino, Du, Wenbin, Shao, Jiahui, Shen, Qirong, and Zhang, Ruifu
- Subjects
- *
CHEMORECEPTORS , *RHIZOBACTERIA , *BACILLUS subtilis , *CHEMOTAXIS , *PLANT exudates - Abstract
Summary: Chemotaxis to plant root exudates is supposed to be a prerequisite for efficient root colonization by rhizobacteria. This is a highly multifactorial process since root exudates are complex compound mixtures of which components are recognized by different chemoreceptors. Little information is available as to the key components in root exudates and their receptors that drive colonization related chemotaxis. We present here the first global assessment of this issue using the plant growth‐promoting rhizobacterium (PGPR) Bacillus velezensis SQR9 (formerly B. amyloliquefaciens). This strain efficiently colonizes cucumber roots, and here, we show that chemotaxis to cucumber root exudates was essential in this process. We conducted chemotaxis assays using cucumber root exudates at different concentrations, individual exudate components as well as recomposed exudates, taking into account their concentrations detected in root exudates. Results indicated that two key chemoreceptors, McpA and McpC, were essential for root exudate chemotaxis and root colonization. Both receptors possess a broad ligand range and recognize most of the exudate key components identified (malic, fumaric, gluconic and glyceric acids, Lys, Ser, Ala and mannose). The remaining six chemoreceptors did not contribute to exudate chemotaxis. This study provides novel insight into the evolution of the chemotaxis system in rhizobacteria. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
41. Mover design and characteristics analysis of 2DoFDDIM.
- Author
-
Wang, Peixing, Si, Jikai, Feng, Haichao, Hu, Yihua, and Cao, Wenping
- Abstract
Two degree‐of‐freedom direct drive induction motor (2DoFDDIM), capable of rotary, linear and helical motion, has widespread application. A new mover structure is proposed, which is made from a hollow cylinder with copper cast in the axial slots and the circumferential slots on its surface. Then, three‐dimensional finite element models of 2DoFDDIM are used to determine the performances of rotary, linear and helical motion developed by the motor. The results show that the new mover has a great improvement on the motor performances of all modes of motions compared with the initial mover. The researches on mover structure and characteristics of 2DoFDDIM present a new path of optimisation on 2DoFIM. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
42. Performance analysis of the 2DoF direct drive induction motor applying composite multilayer method.
- Author
-
Si, Jikai, Xie, Lujia, Cao, Wenping, Hu, Yihua, and Feng, Haichao
- Abstract
This study presents a composite multilayer method (CMM) to evaluate the performance of a two‐degree‐of‐freedom (2DoF) direct drive induction motor (2DoFDDIM) whose solid rotor is coated with a copper layer. It includes a rotary part and a linear part. Based on the traditional multilayer theory, a complete 2DoFDDIM CMM computer program importing propagation constants is built. Due to the complex magnetic field in a 2DoFDDIM, this study mainly analyses it from the perspective of a single DOF motor. An equivalent circuit for the rotary part of the 2DoFDDIM is then derived applying CMM and the 2D magnetic field distribution is obtained by solving Maxwell's equations in motor layers. The developed torques, power factors and stator currents of the rotary part with different slips and the latter two of the linear part at zero speed are calculated by CMM, which are then compared with results from the finite element method (FEM) and experimental results. The computation time of the CMM is far less than that of the FEM. The acceptable accuracy confirms the effectiveness of the CMM for analysis and performance calculations of the 2DoFDDIM. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
43. Design and Analysis of a 2-DOF Split-Stator Induction Motor.
- Author
-
Si, Jikai, Feng, Haichao, Ai, Liwang, Hu, Yihua, and Cao, Wenping
- Subjects
- *
DEGREES of freedom , *ACTUATOR design & construction , *MACHINE tool manufacturing , *ELECTROMAGNETISM , *FINITE element method - Abstract
A two degrees of freedom (2-DOF) actuator capable of producing linear translation, rotary motion, or helical motion would be a desirable asset to the fields of machine tools, robotics, and various apparatuses. In this paper, a novel 2-DOF split-stator induction motor was proposed and electromagnetic structure parameters of the motor were designed and optimized. The feature of the direct-drive 2-DOF induction motor lies in its solid mover arrangement. In order to study the complex distribution of the eddy current field on the ferromagnetic cylinder mover and the motor's operating characteristics, the mathematical model of the proposed motor was established, and characteristics of the motor were analyzed by adopting the permeation depth method (PDM) and finite element method (FEM). The analytical and numerical results from motor simulation clearly show a correlation between the PDM and FEM models. This may be considered as a fair justification for the proposed machine and design tools. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
44. Chemotaxis of Beneficial Rhizobacteria to Root Exudates: The First Step towards Root–Microbe Rhizosphere Interactions.
- Author
-
Feng, Haichao, Fu, Ruixin, Hou, Xueqin, Lv, Yu, Zhang, Nan, Liu, Yunpeng, Xu, Zhihui, Miao, Youzhi, Krell, Tino, Shen, Qirong, and Zhang, Ruifu
- Subjects
- *
PLANT growth , *CHEMOTAXIS , *PLANT exudates , *RHIZOBACTERIA , *RHIZOSPHERE , *PLANT growth-promoting rhizobacteria , *PLANT-microbe relationships , *PLANT protection - Abstract
Chemotaxis, the ability of motile bacteria to direct their movement in gradients of attractants and repellents, plays an important role during the rhizosphere colonization by rhizobacteria. The rhizosphere is a unique niche for plant–microbe interactions. Root exudates are highly complex mixtures of chemoeffectors composed of hundreds of different compounds. Chemotaxis towards root exudates initiates rhizobacteria recruitment and the establishment of bacteria–root interactions. Over the last years, important progress has been made in the identification of root exudate components that play key roles in the colonization process, as well as in the identification of the cognate chemoreceptors. In the first part of this review, we summarized the roles of representative chemoeffectors that induce chemotaxis in typical rhizobacteria and discussed the structure and function of rhizobacterial chemoreceptors. In the second part we reviewed findings on how rhizobacterial chemotaxis and other root–microbe interactions promote the establishment of beneficial rhizobacteria-plant interactions leading to plant growth promotion and protection of plant health. In the last part we identified the existing gaps in the knowledge and discussed future research efforts that are necessary to close them. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
45. Comparison of the Electromagnetic Characteristics of a Novel Gramme Winding and a Concentrated Winding Tubular Permanent-Magnet Linear Generator.
- Author
-
Feng, Haichao, Wei, Jinsong, Si, Jikai, Cheng, Zhiping, Gan, Chun, and Xu, Xiaozhuo
- Subjects
- *
PERMANENT magnet generators , *AIR gap flux , *POWER density , *WAVE energy , *ENERGY conversion , *BRIDGE circuits - Abstract
A tubular permanent-magnet linear generator is suitable for direct drive wave energy conversion owing to its simple structure, easy maintenance and high efficiency. In this study, a novel Gramme winding tubular permanent-magnet linear generator (GW) is designed to increase power density. First, the structure of the Gramme winding and concentrated winding tubular permanent-magnet linear generator is introduced and the operation principle of the generator is analysed. Second, the initial parameters of GW are determined based on the similar overall dimensions to those of the concentrated winding tubular permanent-magnet linear generator. Third, the air gap flux density, external characteristics, voltage regulation, output power, and loss of the two generators are compared. Finally, the DC load capacities of the two generators are compared based on a three-phase full-control bridge rectifying circuit. The results show that the GW has a higher power density than the latter. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
46. Antibiotic Bacillomycin D Affects Iron Acquisition and Biofilm Formation in Bacillus velezensis through a Btr-Mediated FeuABC-Dependent Pathway.
- Author
-
Xu, Zhihui, Mandic-Mulec, Ines, Zhang, Huihui, Liu, Yan, Sun, Xinli, Feng, Haichao, Xun, Weibing, Zhang, Nan, Shen, Qirong, and Zhang, Ruifu
- Abstract
Bacillus spp. produce a wide range of secondary metabolites, including antibiotics, which have been well studied for their antibacterial properties but less so as signaling molecules. Previous results indicated that the lipopeptide bacillomycin D is a signal that promotes biofilm development of Bacillus velezensis SQR9. However, the mechanism behind this signaling is still unknown. Here, we show that bacillomycin D promotes biofilm development by promoting the acquisition of iron. Bacillomycin D promotes the transcription of the iron ABC transporter FeuABC by binding to its transcription factor, Btr. These actions increase intracellular iron concentration and activate the KinB-Spo0A-SinI-SinR-dependent synthesis of biofilm matrix components. We demonstrate that this strategy is beneficial for biofilm development and competition with the Pseudomonas fluorescens PF-5. Our results unravel an antibiotic-dependent signaling mechanism that links iron acquisition to biofilm development and ecological competition. • Increasing intracellular iron concentrations serves as a cue for biofilm development • Bacillomycin D controls transcription of the specific iron transport gene feuABC • Bacillomycin D directly binds to the iron transport regulator Btr • Bacillomycin D modulates biofilm formation through the KinB-Spo0A-SinI-SinR pathway Natural lipopeptides are known antibiotics produced by Bacillus spp. Xu et al. demonstrate that the lipopeptide antibiotic bacillomycin D is involved in biofilm formation by promoting the acquisition of iron. Versatile functions of bacillomycin D in B. velezensis contribute to the microbe's ability to compete in its ecosystem. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
47. Mode Recognition and Fault Positioning of Permanent Magnet Demagnetization for PMSM.
- Author
-
Gao, Caixia, Nie, Yanjie, Si, Jikai, Fu, Ziyi, and Feng, Haichao
- Subjects
PERMANENT magnet motors ,DEMAGNETIZATION ,PERMANENT magnets ,FAULT tolerance (Engineering) ,SYNCHRONOUS electric motors - Abstract
This paper proposes a demagnetization fault detection, mode recognition, magnetic pole positioning, and degree evaluation method for permanent magnet synchronous motors. First, the analytical model of the single-coil no-load back electromotive force (EMF) of demagnetization fault for Permanent magnet synchronous motor (PMSM) arbitrary magnetic poles is established. In the analytical model, the single-coil no-load back EMF residual of the health state and the single magnetic pole sequential demagnetization fault are calculated and normalized. Model results are used as the fault sample database. Second, the energy interval database of the single-coil no-load back EMF residual with different numbers of magnetic pole demagnetization is established. Demagnetization fault detection and degree evaluation are performed by the real-time acquired amplitudes of the single-coil no-load back EMF residual. The number of demagnetization poles is determined by comparing the energy of the single-coil no-load back EMF residual with the energy interval database. Demagnetization mode recognition and magnetic pole positioning are realized by analyzing the correlation coefficients between normalized the single-coil no-load back EMF residual and the fault sample database. Finally, results of analysis of the finite element simulation validate the feasibility and effectiveness of the proposed method. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
48. Rotor Eddy Current Loss Calculation of a 2DoF Direct-Drive Induction Motor.
- Author
-
Wu, Wei, Si, Jikai, Feng, Haichao, Cheng, Zhiping, Hu, Yihua, and Gan, Chun
- Subjects
INDUSTRIAL robots ,AIR gap flux ,ROTORS ,MAXWELL equations ,MAGNETIC fields - Abstract
A two-degree-of-freedom direct-drive induction motor (2DoFDDIM), whose solid rotor is coated with a copper layer, is capable of linear, rotary, and helical motions and has widespread applications. For solid-rotor motors, the calculation and analysis of rotor total eddy current loss (TECL) are crucial in studying the factors causing such a loss and possible loss reduction methods. In this study, a new nonlinear analytical method considering the saturation of the rotor core is proposed to solve the fundamental magnetic field. The new method divides the time period into segments. The magnetic field distribution at any time is obtained using Maxwell equations. The eddy current losses in the copper layer and rotor core caused by the fundamental magnetic field are calculated. The surface eddy current losses in the copper layer and rotor core caused by harmonics are calculated using a 2D analytical method. TECL is determined by the sum of eddy current and surface eddy current losses. Coefficients are utilized to consider eddy, saturation, and end-region effects when calculating the rotor core TECL. The new method is verified using 3D FEM, and the results show the proposed method has higher accuracy than the original method. The errors of the rotor core and copper layer TECLs are less than 6 % and 7.3 % , respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
49. Rhizosphere microbiome assembly involves seed-borne bacteria in compensatory phosphate solubilization.
- Author
-
Shao, Jiahui, Miao, Youzhi, Liu, Kaiming, Ren, Yi, Xu, Zhihui, Zhang, Nan, Feng, Haichao, Shen, Qirong, Zhang, Ruifu, and Xun, Weibing
- Subjects
- *
PLANT breeding , *DNA sequencing , *SOLUBILIZATION , *RHIZOSPHERE , *FUNGAL communities , *PLANT nutrients - Abstract
The rhizosphere microbiome plays a key role in plant nutrient provision. However, the impacts of the vertically transmitted seed microbiome on the assembly of the rhizosphere microbiome and consequently on the functional traits remain unclear. Here, to assess the role of seed-borne beneficial microbes on the assembly of rhizosphere microbiome, we conducted a guest-soil cultivation study using two maize cultivars and their corresponding native soils with different available nutrient contents. The assembly of the rhizosphere bacterial and fungal communities was assessed by amplicon DNA sequencing. Our results demonstrated that the soil microbiome dominates the assembly of the rhizosphere microbiome. A seed-borne beneficial bacterium, Burkholderia gladioli , was able to colonize the rhizosphere in great relative abundance, which significantly altered the assembly of the rhizosphere microbiome. Genome analysis demonstrated that this seed-borne beneficial bacterial species may contain extracellular phosphatase-encoding genes, and gene quantification confirmed their existence, suggesting the possible compensation of phosphate solubilizing function. This functional compensation, which is probably a response to the low concentration of soil available phosphorus, may consequently promote phosphorus acquisition of the host plant. Overall, this study provides new insights into our understanding of the assembly of the rhizosphere microbiome, suggesting that the seed microbiome serves as a functional compensation reservoir to enhance the fitness of progeny plants and highlighting the necessity to consider the seed microbiome during plant breeding. ●Plant seeds possess beneficial microbial storage as a functional compensation reservoir. ●Seed-borne bacteria contribute to the composition of the rhizosphere microbiome. ●A seed-borne P solubilizing bacterium improves rhizospheric function in low P soil. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
50. Integrated Hfq-interacting RNAome and transcriptomic analysis reveals complex regulatory networks of nitrogen fixation in root-associated Pseudomonas stutzeri A1501.
- Author
-
Lv F, Zhan Y, Feng H, Sun W, Yin C, Han Y, Shao Y, Xue W, Jiang S, Ma Y, Hu H, Wei J, Yan Y, and Lin M
- Subjects
- RNA, Bacterial genetics, RNA, Bacterial metabolism, Gene Expression Profiling, Gene Regulatory Networks, Bacterial Proteins genetics, Bacterial Proteins metabolism, High-Throughput Nucleotide Sequencing, Transcriptome, Rhizosphere, Pseudomonas stutzeri genetics, Pseudomonas stutzeri metabolism, Host Factor 1 Protein genetics, Host Factor 1 Protein metabolism, Nitrogen Fixation genetics, Gene Expression Regulation, Bacterial, Plant Roots microbiology
- Abstract
The RNA chaperone Hfq acts as a global regulator of numerous biological processes, such as carbon/nitrogen metabolism and environmental adaptation in plant-associated diazotrophs; however, its target RNAs and the mechanisms underlying nitrogen fixation remain largely unknown. Here, we used enhanced UV cross-linking immunoprecipitation coupled with high-throughput sequencing to identify hundreds of Hfq-binding RNAs probably involved in nitrogen fixation, carbon substrate utilization, biofilm formation, and other functions. Collectively, these processes endow strain A1501 with the requisite capabilities to thrive in the highly competitive rhizosphere. Our findings revealed a previously uncharted landscape of Hfq target genes. Notable among these is nifM , encoding an isomerase necessary for nitrogenase reductase solubility; amtB , encoding an ammonium transporter; oprB , encoding a carbohydrate porin; and cheZ , encoding a chemotaxis protein. Furthermore, we identified more than 100 genes of unknown function, which expands the potential direct regulatory targets of Hfq in diazotrophs. Our data showed that Hfq directly interacts with the mRNA of regulatory proteins (RsmA, AlgU, and NifA), regulatory ncRNA RsmY, and other potential targets, thus revealing the mechanistic links in nitrogen fixation and other metabolic pathways., Importance: Numerous experimental approaches often face challenges in distinguishing between direct and indirect effects of Hfq-mediated regulation. New technologies based on high-throughput sequencing are increasingly providing insight into the global regulation of Hfq in gene expression. Here, enhanced UV cross-linking immunoprecipitation coupled with high-throughput sequencing was employed to identify the Hfq-binding sites and potential targets in the root-associated Pseudomonas stutzeri A1501 and identify hundreds of novel Hfq-binding RNAs that are predicted to be involved in metabolism, environmental adaptation, and nitrogen fixation. In particular, we have shown Hfq interactions with various regulatory proteins' mRNA and their potential targets at the posttranscriptional level. This study not only enhances our understanding of Hfq regulation but, importantly, also provides a framework for addressing integrated regulatory network underlying root-associated nitrogen fixation., Competing Interests: The authors declare no conflict of interest.
- Published
- 2024
- Full Text
- View/download PDF
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