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The application of bio-based solvents for living radical polymerization has been a hot topic in recent year. In this article, γ-valerolactone (GVL), a bio-based solvent as green media for ARGET atom transfer radical polymerization (ATRP) of styrene (St) were investigated. We first conducted polymerization of St in γ-valerolactone using copper(ii) bromide as the catalyst, tris(2-pyridylmethyl) amine as the ligand, and only sodium ascorbate as reducing agent. The polymerization achieved moderate conversion; however, the controllability of polymerization was not very good, providing a polymer with a broad molecular weight distribution (M w/M n > 1.30). When sodium carbonate is introduced, excellent results were obtained providing high yields and low M w/M n values under very low catalyst usage (∼5 ppm). 1H NMR spectroscopy, chain extension, and MALDI–MS experiments confirmed the final polymer chains with high fidelity. The use of GVL solvent opens a new route for the easy synthesis of PS through ARGET ATRP with low catalyst usage conditions.

With the development of clinical informatization technology, a large amount of data resources has been accumulated in the medical field, among which Athletes’ electronic medical records (EMRs) are one of the important data sources of clinical informatization and contain rich medical knowledge, how to obtain valuable biometric features from these data has become the basis of medical intelligence research. Therefore, this paper takes structured text as the entry point, and firstly, the research status of information extraction is elaborated. Moreover, in the work of e-medical biometrics recognition, medical text is added to the language model BERT for pre-training and fine-tuning, and a multi-head self-attention mechanism is introduced to incorporate a bidirectional LSTM model for feature extraction, and biometric features are extracted by using a stochastic conditional field as a classification constraint. This design bypasses the character-level image segmentation step for text line images, thus avoiding the overall accuracy degradation caused by the backward accumulation of errors in character segmentation. Finally, the experimental results show that the model can effectively accomplish the related biological feature extraction tasks. [ABSTRACT FROM AUTHOR]

Purpose: The clinical outcomes of patients who received a cervical collar after anterior cervical decompression and fusion were evaluated by comparison with those of patients who did not receive a cervical collar. Methods: All of the comparative studies published in the PubMed, Cochrane Library, Medline, Web of Science, and EMBASE databases as of 1 October 2023 were included. All outcomes were analysed using Review Manager 5.4. Results: Four studies with a total of 406 patients were included, and three of the studies were randomized controlled trials. Meta-analysis of the short-form 36 results revealed that wearing a cervical collar after anterior cervical decompression and fusion was more beneficial (P < 0.05). However, it is important to note that when considering the Neck Disability Index at the final follow-up visit, not wearing a cervical collar was found to be more advantageous. There were no statistically significant differences in postoperative cervical range of motion, fusion rate, or neck disability index at 6 weeks postoperatively (all P > 0.05) between the cervical collar group and the no cervical collar group. Conclusions: This systematic review and meta-analysis revealed no significant differences in the 6-week postoperative cervical range of motion, fusion rate, or neck disability index between the cervical collar group and the no cervical collar group. However, compared to patients who did not wear a cervical collar, patients who did wear a cervical collar had better scores on the short form 36. Interestingly, at the final follow-up visit, the neck disability index scores were better in the no cervical collar group than in the cervical collar group. PROSPERO registration number: CRD42023466583. [ABSTRACT FROM AUTHOR]

With increasing demand for scratch-resistant flexible electronics, the development of transparent coatings with good scratch resistance and self-healing properties has emerged as a key research topic. In this study, a high-strength self-healing poly(urethane – urea) (PUU)-based nanocomposite coating was prepared by introducing functionalized nanodiamond (ND) into a PUU matrix via solution blending. The PUU matrix had hard-segment repeating units and was constructed using isophorone diamine and isophorone isocyanate. The ND particles were modified using a silane coupling agent, 3-aminopropyltriethoxysilane, to obtain well-dispersed KH-ND nanoparticles. KH-ND promoted microphase separation in the PU matrix, inducing the formation of dense and homogeneous hard domains that dissipated stress, prevented further crack development, and improved the mechanical properties and scratch resistance of the coating. In addition, the coating exhibited excellent self-healing properties. Fourier-transform infrared spectroscopy, scanning electron microscopy, and atomic force microscopy were used to characterize the self-healing and hardening mechanisms of the coating. The environmentally friendly PUU/KH-ND coating is easy to prepare and has broad application prospects in transparent and anti-scratch coatings for flexible electronics, automobiles, and home appliances. [ABSTRACT FROM AUTHOR]

Wakes produced by underwater vehicles, particularly submarines, in density-stratified fluids play a pivotal role across military, academic, and engineering domains. In comparison to homogeneous fluid environments, wakes in stratified flows exhibit distinctive phenomena, including upstream blocking, pancake eddies, internal waves, and variations in hydrodynamic performance. These phenomena are crucial for optimizing the operation of underwater vehicles. This review critically assesses the hydrodynamic and thermodynamic aspects of these wakes through an integration of theoretical, experimental, and numerical approaches. The hydrodynamic wake evolution, comprising near-wake, non-equilibrium, and quasi-two-dimensional regimes, is scrutinized. The underlying physics, encompassing energy transformation, vertical motion suppression, and momentum dissipation, are analyzed in detail. Special emphasis is placed on numerical methods, encompassing diverse approaches and turbulence models and highlighting their differences in fidelity and computational cost. Numerical simulations not only provide insights into the intricate interplay among various factors but also emerge as a crucial focal point for future research directions. In the realm of thermodynamic wakes, we delve into the thermal wake induced by the discharge of high-temperature cooling water and the cold wake resulting from the stirring of seawater. The generation, evolution, and ascent to the free surface of these wakes are explored. Additionally, this review identifies and analyzes current research shortcomings in each aspect. By systematically addressing existing knowledge gaps, our study contributes novel insights that propel academic progress and bear significant implications for submarine engineering. This work not only enhances our understanding of the intricate dynamics involved but also provides a foundation for future research endeavors in this critical field. [ABSTRACT FROM AUTHOR]

Objective: Although radiation workers are exposed to much lower doses of neutron-γ rays than those suffered in nuclear explosions and accidents, it does not mean that their health is not affected by radiation. Lower doses of radiation do not always cause morphological aberrations in chromosomes, so more sophisticated tests must be sought to specific alterations in the exposed cells. Our goal was to characterize the specific gene expression in lymphocytes from logging workers who were continuously exposed to low doses of neutron-γ radiation. We hypothesized that the combination of cell type-specific transcriptomes and open chromatin profiles would identify lymphocyte-specific gene alterations induced by long-term radiation with low-dose neutron^-rays and discover new regulatory pathways and transcriptional regulatory elements. Methods: Lymphocytes were extracted from workers who have been occupationally exposed to neutron-γ and workers unexposed to radiation in the same company. mRNA-seq and ATAC-seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing) were performed, followed integrative analysis to identify specific gene regulatory regions induced by neutron-γ radiation. A qPCR assay was then performed to verify the downregulation of RNA coding for ribosomal proteins and flow cytometry was used to detect ribosomal protein expression and cell cycle alterations. Results: We identified transcripts that were specifically induced by neutron-γ radiation and discovered differential open chromatin regions that correlated with these gene activation patterns. Notably, we observed a downward trend in the expression of both differentially expressed genes and open chromatin peaks. Our most significant finding was that the differential peak upregulated in ATAC-seq, while the differential gene was downregulated in the ribosome pathway. We confirmed that neutron-γ radiation leads to transcriptional inhibition by analyzing the most enriched promoters, examining RPS18 and RPS27A expression by qPCR, and analyzing protein-protein interactions of the differential genes. Ribosomal protein expression and cell cycle were also affected by neutron-γ as detected by flow cytometry. Conclusion: We have comprehensively analyzed the genetic landscape of human lymphocytes based on chromatin accessibility and transcript levels, enabling the identification of novel neutron-γ induced signature genes not previously known. By comparing fine-mapping of open chromatin and RNA reads, we have determined that neutron-γ specifically leads to downregulation of genes in the ribosome pathway, with pseudogenes potentially playing a crucial role. [ABSTRACT FROM AUTHOR]

Simple Summary: Adventitious root formation is a significant limiting factor in the vegetative propagation of economically important plants. Owing to the low germination rates and survival of conventional seed propagation, vegetative propagation remains the best option for large-scale production. Generally, there is a lack of competency for elite plant species cuttings or explants to form adventitious roots. The molecular-level studies of the adventitious root formation have not been explored in many species, such as ramie. As the WUSCEL-related homeobox gene family plays a critical role in promoting vegetative organs and stem cell functioning, we investigated their potential role in adventitious root formation in ramie using genome-wide characterization, gene structure, and expression analysis. The overall result indicated their possible involvement in rooting ramie cuttings. This study thus increases our understanding of the role of these genes and lays a foundation for further studies. A WUSCHEL-related homeobox (WOX) gene family has been implicated in promoting vegetative organs to embryonic transition and maintaining plant embryonic stem cell identity. Using genome-wide analysis, we identified 17 candidates, WOX genes in ramie (Boehmeria nivea). The genes (BnWOX) showed highly conserved homeodomain regions typical of WOX. Based on phylogenetic analysis, they were classified into three distinct groups: modern, intermediate, and ancient clades. The genes displayed 65% and 35% collinearities with their Arabidopsis thaliana and Oryza sativa ortholog, respectively, and exhibited similar motifs, suggesting similar functions. Furthermore, four segmental duplications (BnWOX10/14, BnWOX13A/13B, BnWOX9A/9B, and BnWOX6A/Maker00021031) and a tandem-duplicated pair (BnWOX5/7) among the putative ramie WOX genes were obtained, suggesting that whole-genome duplication (WGD) played a role in WOX gene expansion. Expression profiling analysis of the genes in the bud, leaf, stem, and root of the stem cuttings revealed higher expression levels of BnWOX10 and BnWOX14 in the stem and root and lower in the leaf consistent with the qRT-PCR analysis, suggesting their direct roles in ramie root formation. Analysis of the rooting characteristics and expression in the stem cuttings of sixty-seven different ramie genetic resources showed a possible involvement of BnWOX14 in the adventitious rooting of ramie. Thus, this study provides valuable information on ramie WOX genes and lays the foundation for further research. [ABSTRACT FROM AUTHOR]