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Objectives: This study aimed to pinpoint the universality of extracellular antimicrobial resistance elements (eAREs) and compare the contents of eAREs with those of intracellular AREs (iAREs) in animal feces, thus laying a foundation for the further analysis of the horizontal transfer of antimicrobial resistance genes (ARGs) in the animal guts. Materials and Methods: Extracellular DNAs were isolated from the fecal samples of Pavo cristatus (n = 18), Ursus thibetanus (n = 2), two breeds of broilers (n = 21 and 11, respectively), and from the contents of rabbit intestines (n = 5). eAREs were detected by PCR technology. iAREs in P. cristatus and broiler feces were also detected and compared with the corresponding eAREs. In addition, some gene cassettes of class 1 integrons were sequenced and analyzed. Results: The results showed that eAREs exist in animal feces and intestinal contents. In this study, different eAREs were detected from animal feces and intestinal contents, and tetA, tetB, sul1, sul2, class 1 integron, and IncFIB presented the highest detection rates. The detection rates of certain eAREs were significantly higher than those of parallel iAREs. The integral cassettes with intact structures were found in eAREs, and the cassettes carried ARGs. Conclusions: The presented study here sheds light on the presence of eAREs in animal feces or guts, and eAREs may play an important role in the horizontal gene transfer of ARGs. [J Adv Vet Anim Res 2023; 10(1.000): 103-112]

In efforts to resolve social dilemmas, reinforcement learning is an alternative to imitation and exploration in evolutionary game theory. While imitation and exploration rely on the performance of neighbors, in reinforcement learning individuals alter their strategies based on their own performance in the past. For example, according to the Bush–Mosteller model of reinforcement learning, an individual's strategy choice is driven by whether the received payoff satisfies a preset aspiration or not. Stimuli also play a key role in reinforcement learning in that they can determine whether a strategy should be kept or not. Here we use the Monte Carlo method to study pattern formation and phase transitions towards cooperation in social dilemmas that are driven by reinforcement learning. We distinguish local and global players according to the source of the stimulus they experience. While global players receive their stimuli from the whole neighborhood, local players focus solely on individual performance. We show that global players play a decisive role in ensuring cooperation, while local players fail in this regard, although both types of players show properties of 'moody cooperators'. In particular, global players evoke stronger conditional cooperation in their neighborhoods based on direct reciprocity, which is rooted in the emerging spatial patterns and stronger interfaces around cooperative clusters. [ABSTRACT FROM AUTHOR]

Objectives: This study aimed to pinpoint the universality of extracellular antimicrobial resistance elements (eAREs) and compare the contents of eAREs with those of intracellular AREs (iAREs) in animal feces, thus laying a foundation for the further analysis of the horizontal transfer of antimicrobial resistance genes (ARGs) in the animal guts., Materials and Methods: Extracellular DNAs were isolated from the fecal samples of Pavo cristatus ( n = 18), Ursus thibetanus ( n = 2), two breeds of broilers ( n = 21 and 11, respectively), and from the contents of rabbit intestines ( n = 5). eAREs were detected by PCR technology. iAREs in P. cristatus and broiler feces were also detected and compared with the corresponding eAREs. In addition, some gene cassettes of class 1 integrons were sequenced and analyzed., Results: The results showed that eAREs exist in animal feces and intestinal contents. In this study, different eAREs were detected from animal feces and intestinal contents, and tetA , tetB , sul1 , sul2 , class 1 integron, and IncFIB presented the highest detection rates. The detection rates of certain eAREs were significantly higher than those of parallel iAREs. The integral cassettes with intact structures were found in eAREs, and the cassettes carried ARGs., Conclusions: The presented study here sheds light on the presence of eAREs in animal feces or guts, and eAREs may play an important role in the horizontal gene transfer of ARGs., Competing Interests: The authors declare no conflict of interest., (Copyright: © Journal of Advanced Veterinary and Animal Research.)

Quasi-solid-state polymer electrolytes are one of the most promising candidates for long-life lithium-metal batteries. However, introduction of plasticizers for high ion conductivity at room temperature inevitably gives rise to poor mechanical strength and requires a very thick electrolyte membrane, which is detrimental to safety and energy density of the batteries. Herein, inspired by tube brushes coupling hardness with softness, a novel superstructured polymer bottlebrush BC-g-PLiSTFSI-b-PEGM (BC = bacterial cellulose; PLiSTFSI = poly(lithium 4-styrenesulfonyl-(trifluoromethylsulfonyl) imide); PEGM = poly(diethylene glycol monomethyl ether methacrylate)) with a hard nanofibril backbone and soft functional polymer side-chains is reported as an effective strategy to well balance the mechanical strength and ion conductivity of quasi-solid-state polymer electrolytes. The resulting single lithium-ion conducting quasi-solid-state polymer-brush electrolytes (SLIC-QSPBEs) integrate the features of the ultrathin membrane thickness (10 µm), the nanofibril backbone-strengthened porous nanonetwork (Young's modulus = 1.9 GPa), and the high-rate single lithium-ion conducting diblock copolymer brushes. As a result, the ultrathin yet robust SLIC-QSPBEs enable ultralong-term (over 3300 h) reversible and stable lithium plating/stripping in Li/Li symmetrical cell at a current density of 1 mA cm -2 for lithium anode. This work affords a promising strategy to develop advanced electrolytes for solid-state lithium-metal batteries., (© 2021 Wiley-VCH GmbH.)