Search

In this present work, cement slurry flowing in well annuli with narrow or varied radial sizes is investigated, whose purpose is to precisely calculate the equivalent circulating density (ECD) during well cementing in the petroleum industry. The theoretical Metzner-Reed (MR) method and the direct numerical simulation (DNS) are applied to calculate the cementing ECD for different types of cement slurries by considering both the Bingham and Power-Law rheological models. The cementing ECD calculated by the theoretical MR method is verified to be in accordance with the DNS result. Moreover, for the narrow annulus, the cementing ECD is demonstrated to be sensitive to the rheological model. In addition, based on the obtained DNS results, the accuracies of the cementing ECD calculated by three commercial software, including DRILL BENCH (DB), LANDMARK (LM), and PVI, are compared, to determine their corresponding adaptability for different rheological models. According to the detailed comparisons, LM shows the highest accuracy in calculating the cementing ECD for the Power-Law fluid, while PVI is optimal for the Bingham fluid. However, sharp fluctuations of the calculation errors are observed when DB is applied. [ABSTRACT FROM AUTHOR]

Bisphenol A (BPA) is a common endocrine disruptor and a high-fat diet (HFD) also affects fertility. However, little is known about the long-term consequences of simultaneous exposure to BPA and a HFD on reproductive health. Herein, we assessed the effects of maternal exposure to BPA in combination with a HFD on reproductive function in subsequent generations of female mice and evaluated its effects on the hypothalamic-pituitary-gonadal axis. We found that the combination of maternal exposure to BPA and a HFD led to increased urine BPA levels, precocious puberty, altered estrous cyclicity, decreased follicle numbers, and altered hypothalamic Kiss1 methylation status in F1 and F2 mice. Therefore, we demonstrated that maternal exposure to BPA in combination with a HFD exerts a trans-generational effect on female reproduction. • BPA exerts toxic effects on reproduction and other physiological functions. • Combined maternal BPA and HFD exposure increases urine BPA levels. • Maternal exposure to BPA and a HFD induced dystocia and prolonged pregnancy. • Mice exposed to BPA alone showed high infertility rates. • HFD exacerbates the reproductive dysfunction caused by chronic BPA exposure. [ABSTRACT FROM AUTHOR]

Both bisphenol A (BPA) and high-fat diet (HFD) exert unfavorable effects on animals and humans; moreover, they could affect the health of their offspring. BPA and HFD often coexist in modern lifestyles; however, the long-term effects of simultaneous exposure of mothers to BPA and HFD during the perinatal period on the cardiovascular and metabolic systems of the offspring remain unclear. This study aimed to examine the effect of simultaneous exposure of mothers to BPA and HFD on the risk of metabolic and cardiovascular abnormalities in offspring. Institute of Cancer Research female mice (F0) were exposed to BPA and fed with HFD before and during gestation until the end of lactation. F0 mice were mated with untreated males to produce the first generation (F1); subsequently, adult F1 males/females were mated with normal females/males to produce the second generation (F2). Combined maternal exposure to BPA and HFD caused myocardial hypertrophy and aortic tunica media thickening as well as increased the cross-sectional area of cardiomyocytes and blood pressure in the matrilineal F2 generation. These cardiovascular changes might be associated with reduced endothelial nitric oxide synthase (eNOS) levels. The patrilineal female F2 was more likely to be obese than the patrilineal male F2. Re-feeding with a HFD showed a more significant weight gain and reduced energy expenditure. However, the aforementioned effects were not observed with exposure to HFD or BPA alone during the perinatal period. Our findings suggest that perinatal combinational exposure to BPA and HFD could cause metabolic and cardiovascular disorders in the offspring, Further, our findings demonstrate that the synergistic effects of HFD and BPA could be transmitted to future generations in a sex-dependent manner., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Liu, Liao, Huang, You, Lin, Yang, Fan, Zhong, Li, Li and Xiao.)