20 results on '"Yue, Dong"'
Search Results
2. Self-reported critical gaps in the essential knowledge and capacity of spatial epidemiology between the current university education and competency-oriented professional demands in preparing for a future pandemic among public health postgraduates in China: a nationwide cross-sectional survey
- Author
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Lan, Tao, Cheng, Man, Lin, Yue-Dong, Jiang, Long-Yan, Chen, Ning, Zhu, Man-Tong, Li, Qiao, and Tang, Xian-Yan
- Published
- 2023
- Full Text
- View/download PDF
3. Molecular characterization of PSEUDO RESPONSE REGULATOR family in Rosaceae and function of PbPRR59a and PbPRR59b in flowering regulation.
- Author
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Liu, Zhe, Liu, Weijuan, Wang, Zhangqing, Xie, Zhihua, Qi, Kaijie, Yue, Dong, Li, Yu, Zhang, Shaoling, Wu, Juyou, and Wang, Peng
- Abstract
Background: PSEUDO RESPONSE REGULATOR (PRR) genes are essential components of circadian clock, playing vital roles in multiple processes including plant growth, flowering and stress response. Nonetheless, little is known about the evolution and function of PRR family in Rosaceae species. Results: In this study, a total of 43 PRR genes in seven Rosaceae species were identified through comprehensive analysis. The evolutionary relationships were analyzed with phylogenetic tree, duplication events and synteny. PRR genes were classified into three groups (PRR1, PRR5/9, PRR3/7). The expansion of PRR family was mainly derived from dispersed and whole-genome duplication events. Purifying selection was the major force for PRR family evolution. Synteny analysis indicated the existence of multiple orthologous PRR gene pairs between pear and other Rosaceae species. Moreover, the conserved motifs of eight PbPRR proteins supported the phylogenetic relationship. PRR genes showed diverse expression pattern in various tissues of pear (Pyrus bretschneideri). Transcript analysis under 12-h light/ dark cycle and constant light conditions revealed that PRR genes exhibited distinct rhythmic oscillations in pear. PbPRR59a and PbPRR59b highly homologous to AtPRR5 and AtPRR9 were cloned for further functional verification. PbPRR59a and PbPRR59b proteins were localized in the nucleus. The ectopic overexpression of PbPRR59a and PbPRR59b significantly delayed flowering in Arabidopsis transgenic plants by repress the expression of AtGI, AtCO and AtFT under long-day conditions. Conclusions: These results provide information for exploring the evolution of PRR genes in plants, and contribute to the subsequent functional studies of PRR genes in pear and other Rosaceae species. [ABSTRACT FROM AUTHOR]
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- 2024
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- View/download PDF
4. Root-to-shoot signaling positively mediates source-sink relation in late growth stages in diploid and tetraploid wheat.
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Batool, Asfa, Li, Shi-Sheng, Yue, Dong-Xia, Ullah, Fazal, Zhao, Ling, Cheng, Zheng-Guo, Wang, Chao, Duan, Hai-Xia, Lv, Guang-Chao, Haq, Zeeshan ul, Ahmed, Khalil, Gui, Yan-Wen, Zhu, Li, Xiao, Yun-Li, and Xiong, You-Cai
- Abstract
Non-hydraulic root source signaling (nHRS) is a unique positive response to soil drying in the regulation of plant growth and development. However, it is unclear how the nHRS mediates the tradeoff between source and sink at the late growth stages and its adaptive mechanisms in primitive wheat. To address this issue, a root-splitting design was made by inserting solid partition in the middle of the pot culture to induce the occurrence of nHRS using four wheat cultivars (MO1 and MO4, diploid; DM22 and DM31, tetraploid) as materials. Three water treatments were designed as 1) both halves watered (CK), 2) holistic root system watered then droughted (FS), 3) one-half of the root system watered and half droughted (PS). FS and PS were designed to compare the role of the full root system and split root system to induce nHRS. Leaves samples were collected during booting and anthesis to compare the role of nHRS at both growth stages. The data indicated that under PS treatment, ABA concentration was significantly higher than FS and CK, demonstrating the induction of nHRS in split root design and nHRS decreased cytokinin (ZR) levels, particularly in the PS treatment. Soluble sugar and proline accumulation were higher in the anthesis stage as compared to the booting stage. POD activity was higher at anthesis, while CAT was higher at the booting stage. Increased ABA (nHRS) correlated with source-sink relationships and metabolic rate (i.e., leaf) connecting other stress signals. Biomass density showed superior resource acquisition and utilization capabilities in both FS and PS treatment as compared to CK in all plants. Our findings indicate that nHRS-induced alterations in phytohormones and their effect on source-sink relations were allied with the growth stages in primitive wheat. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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5. Serum electrolyte concentrations and risk of atrial fibrillation: an observational and mendelian randomization study.
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Wu, Yang, Kong, Xiang-Jun, Ji, Ying-Ying, Fan, Jun, Ji, Cheng-Cheng, Chen, Xu-Miao, Ma, Yue-Dong, Tang, An-Li, Cheng, Yun-Jiu, and Wu, Su-Hua
- Subjects
ATRIAL fibrillation ,ELECTROLYTES ,WATER-electrolyte imbalances ,STROKE ,ODDS ratio ,PROGNOSIS - Abstract
Background: Atrial fibrillation (AF) is a prevalent arrhythmic condition resulting in increased stroke risk and is associated with high mortality. Electrolyte imbalance can increase the risk of AF, where the relationship between AF and serum electrolytes remains unclear. Methods: A total of 15,792 individuals were included in the observational study, with incident AF ascertainment in the Atherosclerosis Risk in Communities (ARIC) study. The Cox regression models were applied to calculate the hazard ratio (HR) and 95% confidence interval (CI) for AF based on different serum electrolyte levels. Mendelian randomization (MR) analyses were performed to examine the causal association. Results: In observational study, after a median 19.7 years of follow-up, a total of 2551 developed AF. After full adjustment, participants with serum potassium below the 5th percentile had a higher risk of AF relative to participants in the middle quintile. Serum magnesium was also inversely associated with the risk of AF. An increased incidence of AF was identified in individuals with higher serum phosphate percentiles. Serum calcium levels were not related to AF risk. Moreover, MR analysis indicated that genetically predicted serum electrolyte levels were not causally associated with AF risk. The odds ratio for AF were 0.999 for potassium, 1.044 for magnesium, 0.728 for phosphate, and 0.979 for calcium, respectively. Conclusions: Serum electrolyte disorders such as hypokalemia, hypomagnesemia and hyperphosphatemia were associated with an increased risk of AF and may also serve to be prognostic factors. However, the present study did not support serum electrolytes as causal mediators for AF development. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Multi-level determinants of failure to receive timely and complete measles vaccinations in Southwest China: a mixed methods study
- Author
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Tang, Xian-Yan, Cheng, Man, Geater, Alan, Deng, Qiu-Yun, Zhong, Ge, Lin, Yue-Dong, Chen, Ning, Lan, Tao, Jiang, Long-Yan, Zhu, Man-Tong, and Li, Qiao
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- 2021
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7. Circular RNA circ_0020710 drives tumor progression and immune evasion by regulating the miR-370-3p/CXCL12 axis in melanoma
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Wei, Chuan-Yuan, Zhu, Meng-Xuan, Lu, Nan-Hang, Liu, Jia-Qi, Yang, Yan-Wen, Zhang, Yong, Shi, Yue-Dong, Feng, Zi-Hao, Li, Jia-Xia, Qi, Fa-Zhi, and Gu, Jian-Ying
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- 2020
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8. Investigating the cognitive capacity constraints of an ICU care team using a systems engineering approach
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Jaeyoung Park, Xiang Zhong, Yue Dong, Amelia Barwise, and Brian W. Pickering
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Male ,Patient Care Team ,SARS-CoV-2 ,Research ,COVID-19 ,Workload ,Middle Aged ,Organizational decision making ,Situational awareness ,Systems approach ,Intensive Care Units ,Anesthesiology and Pain Medicine ,Cognition ,Anesthesiology ,Humans ,RD78.3-87.3 ,Female ,Patient Safety ,Cognitive function ,Electronic medical records ,Decision Making, Organizational ,Aged - Abstract
Background ICU operational conditions may contribute to cognitive overload and negatively impact on clinical decision making. We aimed to develop a quantitative model to investigate the association between the operational conditions and the quantity of medication orders as a measurable indicator of the multidisciplinary care team’s cognitive capacity. Methods The temporal data of patients at one medical ICU (MICU) of Mayo Clinic in Rochester, MN between February 2016 to March 2018 was used. This dataset includes a total of 4822 unique patients admitted to the MICU and a total of 6240 MICU admissions. Guided by the Systems Engineering Initiative for Patient Safety model, quantifiable measures attainable from electronic medical records were identified and a conceptual framework of distributed cognition in ICU was developed. Univariate piecewise Poisson regression models were built to investigate the relationship between system-level workload indicators, including patient census and patient characteristics (severity of illness, new admission, and mortality risk) and the quantity of medication orders, as the output of the care team’s decision making. Results Comparing the coefficients of different line segments obtained from the regression models using a generalized F-test, we identified that, when the ICU was more than 50% occupied (patient census > 18), the number of medication orders per patient per hour was significantly reduced (average = 0.74; standard deviation (SD) = 0.56 vs. average = 0.65; SD = 0.48; p p Conclusions Our model suggests that ICU operational factors, such as admission rates and patient severity of illness may impact the critical care team’s cognitive function and result in changes in the production of medication orders. The results of this analysis heighten the importance of increasing situational awareness of the care team to detect and react to changing circumstances in the ICU that may contribute to cognitive overload.
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- 2022
9. Preimplantation genetic diagnosis and screening (PGD/S) using a semiconductor sequencing platform
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Hailiang Liu, Ye-Qing Qian, Min Chen, Yuqin Luo, Bei Liu, Yanmei Yang, Ying-Zhi Huang, Hongge Li, Chun-fang Peng, Min-Yue Dong, Liya Wang, Fan Jin, Dan Chen, Rao Xingqiang, Yinghui Ye, Kai Yan, and Yixi Sun
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Male ,Blastomeres ,Preimplantation genetic diagnosis/screening ,lcsh:QH426-470 ,DNA Copy Number Variations ,lcsh:Medicine ,Computational biology ,Fertilization in Vitro ,Biology ,Preimplantation genetic diagnosis ,Proteomics ,03 medical and health sciences ,Drug Discovery ,Genetics ,Humans ,Copy-number variation ,Molecular Biology ,Preimplantation Diagnosis ,Sex Chromosome Aberrations ,Whole Genome Amplification ,0303 health sciences ,Comparative Genomic Hybridization ,Whole Genome Sequencing ,Copy number variation ,lcsh:R ,030305 genetics & heredity ,food and beverages ,Ion semiconductor sequencing ,Blastomere ,respiratory system ,Aneuploidy ,Human genetics ,Whole genome amplification ,lcsh:Genetics ,Semiconductors ,Array comparative genomic hybridization ,Semiconductor sequencing platform ,Molecular Medicine ,lipids (amino acids, peptides, and proteins) ,Female ,Single-Cell Analysis ,Primary Research ,Comparative genomic hybridization - Abstract
Background Recent advances in semiconductor sequencing platform (SSP) have provided new methods for preimplantation genetic diagnosis/screening (PGD/S). The present study aimed to evaluate the applicability and efficiency of SSP in PGD/S. Methods The artificial positive single-cell-like DNAs and normal single-cell samples were chosen to test our semiconductor sequencing platform for preimplantation genetic diagnosis/screening (SSP-PGD/S) method with two widely used whole-genome amplification (WGA) kits. A total of 557 single blastomeres were collected from in vitro fertilization (IVF) couples, and their WGA products were processed and analyzed by our SSP-PGD/S method in comparison with array comparative genomic hybridization (array-CGH). Results Our SSP-PGD/S method indicated high compatibilities with two commercial WGA kits. For 557 single blastomeres, our method with four million reads in average could detect 24-chromosome aneuploidies as well as microdeletion/microduplication of the size over 4 Mb, providing 100% consistent conclusion with array-CGH method in the classification of whether it was transplantable. Conclusions Our studies suggested that SSP-PGD/S represents a valuable alternative to array-CGH and brought PGD/S into a new era of more rapid, accurate, and economic. Electronic supplementary material The online version of this article (10.1186/s40246-018-0187-x) contains supplementary material, which is available to authorized users.
- Published
- 2019
10. Development and validation of clinical performance assessment in simulated medical emergencies: an observational study
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Ronaldo Sevilla Berrios, John C. O’Horo, Xiaomei Chen, Aysen Erdogan, Lisbeth Garcia Arguello, Oguz Kilickaya, Brian W. Pickering, Christopher N. Schmickl, Rahul Kashyap, Yue Dong, and Ognjen Gajic
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medicine.medical_specialty ,Critical Care ,Task (project management) ,03 medical and health sciences ,0302 clinical medicine ,Cohen's kappa ,medicine ,Humans ,Medical physics ,030212 general & internal medicine ,Prospective Studies ,Program Development ,Reliability (statistics) ,Face validity ,business.industry ,Construct validity ,Rubric ,030208 emergency & critical care medicine ,Checklist ,Emergency medicine ,Emergency Medicine ,Observational study ,Clinical Competence ,business ,Research Article - Abstract
Background Critical illness is a time-sensitive process which requires practitioners to process vast quantities of data and make decisions rapidly. We have developed a tool, the Checklist for Early Recognition and Treatment of Acute Illness (CERTAIN), aimed at enhancing care delivery in such situations. To determine the efficacy of CERTAIN and similar cognitive aids, we developed rubric for evaluating provider performance in a simulated medical resuscitation environments. Methods We recruited 18 clinicians with current valid ACLS certification for evaluation in three simulated medical scenarios designed to mimic typical medical decompensation events routinely experienced in clinical care. Subjects were stratified as experienced or novice based on prior critical care training. A checklist of critical actions was designed using face validity for each scenario to evaluate task completion and performance. Simulation sessions were video recorded and scored by two independent raters. Construct validity was assessed under the assumption that experienced clinicians should perform better than novice clinicians on each task. Reliability was assessed as percentage agreement, kappa statistics and Bland-Altman plots as appropriate. Results Eleven experts and seven novices completed evaluation. The overall agreement on common checklist item completion was 84.8 %. The overall model achieved face validity and was consistent with our construct, with experienced clinicians trending towards better performance compared to novices for accuracy and speed of task completion. Conclusions A standardized video assessment tool has potential to provide a valid and reliable method to assess 12 performances of clinicians facing simulated medical emergencies. Electronic supplementary material The online version of this article (doi:10.1186/s12873-015-0066-x) contains supplementary material, which is available to authorized users.
- Published
- 2016
11. Plague in China 2014-All sporadic case report of pneumonic plague.
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Yun-fang Li, De-biao Li, Hong-sheng Shao, Hong-jun Li, Yue-dong Han, Li, Yun-Fang, Li, De-Biao, Shao, Hong-Sheng, Li, Hong-Jun, and Han, Yue-Dong
- Subjects
PLAGUE ,YERSINIA pestis ,COMMUNICABLE diseases ,PANDEMICS - Abstract
Background: Yersinia pestis is the pathogen of the plague and caused three pandemics worldwide. Pneumonic plague is rarer than bubonic and septicemic plague. We report detailed clinical and pathogenic data for all the three sporadic cases of pneumonic plagues in China in 2014.Case Presentation: All the three patients are herders in Gansu province of China. They were all infected by Yersinia pestis and displayed in the form of pneumonic plague respectively without related. We tested patient specimens from the upper (nasopharyngeal swabs) or the lower (sputum) respiratory tract and whole blood, plasma, and serum specimens for Yersinia pestis. All patients had fever, cough and dyspnea, and for patient 2 and 3, unconscious. Respiratory symptoms were predominant with acute respiratory failure. The chest X-ray showed signs consistent with necrotizing inflammation with multiple lobar involvements. Despite emergency treatment, all patients died of refractory multiple organ failure within 24 h after admission to hospital. All the contacts were quarantined immediately and there were no secondary cases.Conclusions: Nowadays, the plague is epidemic in animals and can infect people who contact with the infected animals which may cause an epidemic in human. We think dogs maybe an intermediate vector for plague and as a source of risk for humans who are exposed to pet animals or who work professionally with canines. If a patient has been exposed to a risk factor and has fever and dyspnea, plague should be considered. People who had contact with a confirmed case should be isolated and investigated for F1 antigen analysis and receive post-exposure preventive treatment. A vaccination strategy might be useful for individuals who are occupationally exposed in areas where endemically infected reservoirs of plague-infected small mammals co-exist. [ABSTRACT FROM AUTHOR]- Published
- 2016
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12. Checklist for Early Recognition and Treatment of Acute Illness (CERTAIN): evolution of a content management system for point-of-care clinical decision support.
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Barwise, Amelia, Garcia-Arguello, Lisbeth, Yue Dong, Hulyalkar, Manasi, Vukoja, Marija, Schultz, Marcus J., Adhikari, Neill K. J., Bonneton, Benjamin, Kilickaya, Oguz, Kashyap, Rahul, Gajic, Ognjen, and Schmickl, Christopher N.
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ACUTE diseases ,MEDICAL care ,MEDICAL personnel ,DECISION support systems ,LISTS ,CRITICAL care medicine ,THERAPEUTICS - Abstract
Background: The Checklist for Early Recognition and Treatment of Acute Illness (CERTAIN) is an international collaborative project with the overall objective of standardizing the approach to the evaluation and treatment of critically ill patients world-wide, in accordance with best-practice principles. One of CERTAIN’s key features is clinical decision support providing point-of-care information about common acute illness syndromes, procedures, and medications in an index card format. Methods: This paper describes 1) the process of developing and validating the content for point-of-care decision support, and 2) the content management system that facilitates frequent peer-review and allows rapid updates of content across different platforms (CERTAIN software, mobile apps, pdf-booklet) and different languages. Results: Content was created based on survey results of acute care providers and validated using an open peer-review process. Over a 3 year period, CERTAIN content expanded to include 67 syndrome cards, 30 procedure cards, and 117 medication cards. 127 (59 %) cards have been peer-reviewed so far. Initially MS Word® and Dropbox® were used to create, store, and share content for peer-review. Recently Google Docs® was used to make the peer-review process more efficient. However, neither of these approaches met our security requirements nor has the capacity to instantly update the different CERTAIN platforms. Conclusion: Although we were able to successfully develop and validate a large inventory of clinical decision support cards in a short period of time, commercially available software solutions for content management are suboptimal. Novel custom solutions are necessary for efficient global point of care content system management. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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13. Development and validation of clinical performance assessment in simulated medical emergencies: an observational study.
- Author
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Erdogan, Aysen, Yue Dong, Xiaomei Chen, Schmickl, Christopher, Sevilla Berrios, Ronaldo A., Garcia Arguello, Lisbeth Y., Kashyap, Rahul, Kilickaya, Oguz, Pickering, Brian, Gajic, Ognjen, O'Horo, John C., Dong, Yue, and Chen, Xiaomei
- Subjects
- *
MEDICAL needs assessment , *MEDICAL emergencies , *CATASTROPHIC illness , *EMERGENCY medical services , *EMERGENCY medicine , *CLINICAL competence , *COMPARATIVE studies , *CRITICAL care medicine , *LONGITUDINAL method , *RESEARCH methodology , *MEDICAL cooperation , *RESEARCH , *RESEARCH funding , *EVALUATION research , *HUMAN services programs - Abstract
Background: Critical illness is a time-sensitive process which requires practitioners to process vast quantities of data and make decisions rapidly. We have developed a tool, the Checklist for Early Recognition and Treatment of Acute Illness (CERTAIN), aimed at enhancing care delivery in such situations. To determine the efficacy of CERTAIN and similar cognitive aids, we developed rubric for evaluating provider performance in a simulated medical resuscitation environments.Methods: We recruited 18 clinicians with current valid ACLS certification for evaluation in three simulated medical scenarios designed to mimic typical medical decompensation events routinely experienced in clinical care. Subjects were stratified as experienced or novice based on prior critical care training. A checklist of critical actions was designed using face validity for each scenario to evaluate task completion and performance. Simulation sessions were video recorded and scored by two independent raters. Construct validity was assessed under the assumption that experienced clinicians should perform better than novice clinicians on each task. Reliability was assessed as percentage agreement, kappa statistics and Bland-Altman plots as appropriate.Results: Eleven experts and seven novices completed evaluation. The overall agreement on common checklist item completion was 84.8 %. The overall model achieved face validity and was consistent with our construct, with experienced clinicians trending towards better performance compared to novices for accuracy and speed of task completion.Conclusions: A standardized video assessment tool has potential to provide a valid and reliable method to assess 12 performances of clinicians facing simulated medical emergencies. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
14. A survey on the resources and practices in pediatric critical care of resource-rich and resource-limited countries.
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Tripathi, Sandeep, Kaur, Harsheen, Kashyap, Rahul, Yue Dong, Gajic, Ognjen, and Murthy, Srinivas
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CRITICAL care medicine ,PEDIATRIC intensive care ,CRITICALLY ill children - Abstract
Background: Contemporary critical care research necessitates involvement of multiple centers, preferably from many countries. Adult and pediatric research networks have produced outstanding data; however, their involvement is restricted to a small percentage of the industrialized nations. Implementation of their findings in low- and middle-income countries (LMICs) is fraught with challenges. Methods: We conducted an online international survey to assess and compare disease burden and resources to participate in multicenter research studies through a listserv of the World Federation of Pediatric Intensive and Critical Care Societies. Respondents were grouped into high-income countries and LMICs on the basis of World Bank classification. Results: Survey was completed by 73 centers in 34 countries (34 from high-income countries and 39 from LMICs). Compared with high-income countries, the pediatric intensive care units in LMICs were characterized by a lower number of critical care specialists, more difficult access to hemodialysis, and a lower number of elective postoperative patients, but a similar overall disease burden. Training and resources for research were comparable in the two cohorts. Conclusions: Although differences exist in access to both trained providers and equipment, the survey results were more striking in their similarity. It is essential that centers from LMICs be included in multinational studies, to generate results applicable to all children worldwide. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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15. BMSCs reduce rat granulosa cell optosis induced by cisplatin and perimenopause.
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Jun-qi Guo, Xia Gao, Zhi-jie Lin, Wei-zhen Wu, Liang-hu Huang, Hui-yue Dong, Jin Chen, Jun Lu, Yun-fen Fu, Jin Wang, Yu-jie Ma, Xiao-wen Chen, Zhi-xian Wu, Fu-qiang He, Shun-liang Yang, Lian-ming Liao, Feng Zheng, and Jian-ming Tan
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MESENCHYMAL stem cells ,BONE marrow ,PERIMENOPAUSE ,GRANULOSA cells ,CISPLATIN ,APOPTOSIS - Abstract
Background: The objective of this study was to evaluate the effect of bone marrow mesenchymal stem cells (BMSCs) on the apoptosis of granulosa cells (GCs) in rats. BMSCs and GCs were isolated from rats. GCs were separated into one of the following three groups: an untreated control group (control), a cisplatin (5 mg/L) treatment group (cisplatin), and group co-cultured with BMSCs and treated with cisplatin (BMSC). GC apoptosis was analyzed by annexin V staining and real-time PCR analysis for apoptosis-related genes. The effect of BMSCs was also determined in 9 to 10 month-old perimenopausal rats that were separated into the following groups: saline control, BMSC transplantation (1-2 × 10+sup>6+/sup> cells), and estrogen treatment (0.158 mg/kg/d) groups. A young group consisting of 3 to 4 month-old rats that were treated with saline was also evaluated as a control. After 1 and 3 months, GC apoptosis was evaluated by TUNEL analysis. Results: Cisplatin increased GC apoptosis from 0.59% to 13.04% in the control and cisplatin treatment groups, respectively, which was significantly reduced upon co-culture with BMSCs to 4.84%. Cisplatin treatment increased p21 and bax and decreased c-myc mRNA expression, which was reversed upon co-culture with BMSCs. As compared to young rats, increased apoptosis was observed in the perimenopausal rats (P + 0.001). After 3 months, the apoptosis rate in the BMSC group was significantly lower than that of the control group (P = 0.007).Conclusions: BMSC therapy may protect against GC apoptosis induced by cisplatin and perimenopause. Further studies are necessary to evaluate therapeutic efficacy of BMSCs. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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16. Bone morphogenetic protein-15 in follicle fluid combined with age may differentiate between successful and unsuccessful poor ovarian responders.
- Author
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Yan-Ting Wu, Ting-Ting Wang, Xi-Jing Chen, Xiao-Ming Zhu, Min-Yue Dong, Jian-Zhong Sheng, Chen-Ming Xu, and He-Feng Huang
- Subjects
BONE morphogenetic proteins ,PREGNANCY ,CONCEPTION ,GERM cells ,EMBRYO transfer - Abstract
Background: The counselling of poor ovarian responders about the probability of pregnancy remains a puzzle for gynaecologists. The aim of this study was to optimise the management of poor responders by investigating the role of the oocyte-derived factor bone morphogenetic protein-15 (BMP-15) combined with chronological age in the prediction of the outcome of in-vitro fertilisation-embryo transfer (IVF-ET) in poor responders. Methods: A retrospective study conducted in a university hospital. A total of 207 poor ovarian responders who reached the ovum pick-up stage undergoing IVF/intracytoplasmic sperm injection (ICSI) with three or fewer follicles no less than 14 mm on the day of oocyte retrieval were recruited from July 1, 2008 to December 31, 2009. Another 215 coinstantaneous cycles with normal responses were selected as controls. The BMP-15 levels in the follicular fluid (FF) of the 207 poor responders were analysed by western blot. Based on the FF BMP-15 level and age, poor responders were sub-divided into four groups. The main outcome measures were the FF BMP-15 level, implantation rate, pregnancy rate, and live birth rate. Results: The implantation rate (24.2% vs. 15.3%), chemical pregnancy rate (40% vs. 23.7%), clinical pregnancy rate (36.5% vs. 20.4%) and live birth rate (29.4% vs. 15.1%) in the high BMP-15 group were significantly higher than those in the low BMP-15 group. Furthermore, poor responders aged less than or equal to 35 years with a higher FF BMP-15 level had the best implantation, pregnancy and live birth rates, which were comparable with those of normal responders. Conclusions: Our study suggests a potential role of BMP-15 in the prediction of the IVF outcome. A high FF BMP-15 combined with an age less than or equal to 35 years may be used as a potential indicator for repeating IVF cycles in poor ovarian responders. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
17. Plague in China 2014-All sporadic case report of pneumonic plague.
- Author
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Li YF, Li DB, Shao HS, Li HJ, and Han YD
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- Adult, China, Fatal Outcome, Humans, Male, Middle Aged, Plague complications, Plague diagnosis
- Abstract
Background: Yersinia pestis is the pathogen of the plague and caused three pandemics worldwide. Pneumonic plague is rarer than bubonic and septicemic plague. We report detailed clinical and pathogenic data for all the three sporadic cases of pneumonic plagues in China in 2014., Case Presentation: All the three patients are herders in Gansu province of China. They were all infected by Yersinia pestis and displayed in the form of pneumonic plague respectively without related. We tested patient specimens from the upper (nasopharyngeal swabs) or the lower (sputum) respiratory tract and whole blood, plasma, and serum specimens for Yersinia pestis. All patients had fever, cough and dyspnea, and for patient 2 and 3, unconscious. Respiratory symptoms were predominant with acute respiratory failure. The chest X-ray showed signs consistent with necrotizing inflammation with multiple lobar involvements. Despite emergency treatment, all patients died of refractory multiple organ failure within 24 h after admission to hospital. All the contacts were quarantined immediately and there were no secondary cases., Conclusions: Nowadays, the plague is epidemic in animals and can infect people who contact with the infected animals which may cause an epidemic in human. We think dogs maybe an intermediate vector for plague and as a source of risk for humans who are exposed to pet animals or who work professionally with canines. If a patient has been exposed to a risk factor and has fever and dyspnea, plague should be considered. People who had contact with a confirmed case should be isolated and investigated for F1 antigen analysis and receive post-exposure preventive treatment. A vaccination strategy might be useful for individuals who are occupationally exposed in areas where endemically infected reservoirs of plague-infected small mammals co-exist.
- Published
- 2016
- Full Text
- View/download PDF
18. A Bayesian decision fusion approach for microRNA target prediction.
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Yue D, Guo M, Chen Y, and Huang Y
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- Animals, Bayes Theorem, Genome, Human, Humans, Internet, Mice, Proteins genetics, Proteins metabolism, Proteomics, ROC Curve, Rats, User-Computer Interface, Algorithms, MicroRNAs metabolism
- Abstract
MicroRNAs (miRNAs) are 19-25 nucleotides non-coding RNAs known to have important post-transcriptional regulatory functions. The computational target prediction algorithm is vital to effective experimental testing. However, since different existing algorithms rely on different features and classifiers, there is a poor agreement among the results of different algorithms. To benefit from the advantages of different algorithms, we proposed an algorithm called BCmicrO that combines the prediction of different algorithms with Bayesian Network. BCmicrO was evaluated using the training data and the proteomic data. The results show that BCmicrO improves both the sensitivity and the specificity of each individual algorithm. All the related materials including genome-wide prediction of human targets and a web-based tool are available at http://compgenomics.utsa.edu/gene/gene_1.php.
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- 2012
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19. A Bayesian approach for identifying miRNA targets by combining sequence prediction and gene expression profiling.
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Liu H, Yue D, Zhang L, Chen Y, Gao SJ, and Huang Y
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- 3' Untranslated Regions, Algorithms, Bayes Theorem, Gene Expression Regulation, Humans, MicroRNAs chemistry, Oligonucleotide Array Sequence Analysis, User-Computer Interface, Gene Expression Profiling, MicroRNAs metabolism
- Abstract
Background: MicroRNAs (miRNAs) are single-stranded non-coding RNAs shown to plays important regulatory roles in a wide range of biological processes and diseases. The functions and regulatory mechanisms of most of miRNAs are still poorly understood in part because of the difficulty in identifying the miRNA regulatory targets. To this end, computational methods have evolved as important tools for genome-wide target screening. Although considerable work in the past few years has produced many target prediction algorithms, most of them are solely based on sequence, and the accuracy is still poor. In contrast, gene expression profiling from miRNA transfection experiments can provide additional information about miRNA targets. However, most of existing research assumes down-regulated mRNAs as targets. Given the fact that the primary function of miRNA is protein inhibition, this assumption is neither sufficient nor necessary., Results: A novel Bayesian approach is proposed in this paper that integrates sequence level prediction with expression profiling of miRNA transfection. This approach does not restrict the target to be down-expressed and thus improve the performance of existing target prediction algorithm. The proposed algorithm was tested on simulated data, proteomics data, and IP pull-down data and shown to achieve better performance than existing approaches for target prediction. All the related materials including source code are available at http://compgenomics.utsa.edu/expmicro.html., Conclusions: The proposed Bayesian algorithm integrates properly the sequence paring data and mRNA expression profiles for miRNA target prediction. This algorithm is shown to have better prediction performance than existing algorithms.
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- 2010
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20. Improving performance of mammalian microRNA target prediction.
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Liu H, Yue D, Chen Y, Gao SJ, and Huang Y
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- 5' Untranslated Regions, Animals, Gene Silencing, Genome, Humans, Mammals genetics, Mammals metabolism, MicroRNAs metabolism, Proteomics, RNA, Untranslated chemistry, RNA, Untranslated metabolism, 3' Untranslated Regions, Algorithms, MicroRNAs chemistry
- Abstract
Background: MicroRNAs (miRNAs) are single-stranded non-coding RNAs known to regulate a wide range of cellular processes by silencing the gene expression at the protein and/or mRNA levels. Computational prediction of miRNA targets is essential for elucidating the detailed functions of miRNA. However, the prediction specificity and sensitivity of the existing algorithms are still poor to generate meaningful, workable hypotheses for subsequent experimental testing. Constructing a richer and more reliable training data set and developing an algorithm that properly exploits this data set would be the key to improve the performance current prediction algorithms., Results: A comprehensive training data set is constructed for mammalian miRNAs with its positive targets obtained from the most up-to-date miRNA target depository called miRecords and its negative targets derived from 20 microarray data. A new algorithm SVMicrO is developed, which assumes a 2-stage structure including a site support vector machine (SVM) followed by a UTR-SVM. SVMicrO makes prediction based on 21 optimal site features and 18 optimal UTR features, selected by training from a comprehensive collection of 113 site and 30 UTR features. Comprehensive evaluation of SVMicrO performance has been carried out on the training data, proteomics data, and immunoprecipitation (IP) pull-down data. Comparisons with some popular algorithms demonstrate consistent improvements in prediction specificity, sensitivity and precision in all tested cases. All the related materials including source code and genome-wide prediction of human targets are available at http://compgenomics.utsa.edu/svmicro.html., Conclusions: A 2-stage SVM based new miRNA target prediction algorithm called SVMicrO is developed. SVMicrO is shown to be able to achieve robust performance. It holds the promise to achieve continuing improvement whenever better training data that contain additional verified or high confidence positive targets and properly selected negative targets are available.
- Published
- 2010
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