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Pleiotropic drug resistance (PDR) transporters, which are part of the ABCG subfamily of ATP-binding cassette (ABC) transporters, have been found to be involved in heavy metal tolerance. Salix species (willow) is widely regarded as a perfect candidate for phytoremediation of heavy metals because of its substantial biomass, strong tolerance, and remarkable capacity to accumulate heavy metals. However, the phylogeny and mechanisms underlying the response to heavy metals within the PDR family in willow have yet to be determined. In this study, we discovered and valuated a total of 21 PDR genes in the genome of Salix purpurea. The phylogenetic relationships of these genes were used to classify them into five major clades. The SpPDRs exhibited variations in exon-intron distribution patterns and gene lengths across different branches. Cis-acting elements linked to stress response, drought induction, low temperature, and defense response were discovered in the promoters of PDRs. Significant variations in the transcription levels of various PDR genes were observed across different tissues under heavy metal stress, with distinct heavy metals regulating different PDR members. In roots, PDR4 and PDR21 exhibited high expression levels. Meanwhile, PDR7 and PDR17 showed similar transcription patterns across all analyzed tissues. Furthermore, there was a significant and positive correlation between PDR5 and PDR16, whereas a significant and negative correlation was detected between PDR3 and PDR9, suggesting that the response of PDR members to heavy metals is complex and multifaceted. These findings will establish a vital basis for comprehending the biological functionalities of PDR genes, specifically their involvement in the regulation of willow's tolerance to heavy metals. [ABSTRACT FROM AUTHOR]

To investigate forest resources, it is necessary to identify the tree species. However, it is a challenge to identify tree species using 3D point clouds of trees collected by light detection and ranging (LiDAR). PointNet++, a point cloud deep learning network, can effectively classify 3D objects. It is important to establish high-quality individual tree point cloud datasets when applying PointNet++ to identifying tree species. However, there are different data processing methods to produce sample datasets, and the processes are tedious. In this study, we suggest how to select the appropriate method by designing comparative experiments. We used the backpack laser scanning (BLS) system to collect point cloud data for a total of eight tree species in three regions. We explored the effect of tree height on the classification accuracy of tree species by using different point cloud normalization methods and analyzed the effect of leaf point clouds on classification accuracy by separating the leaves and wood of individual tree point clouds. Five downsampling methods were used: farthest point sampling (FPS), K-means, random, grid average sampling, and nonuniform grid sampling (NGS). Data with different sampling points were designed for the experiments. The results show that the tree height feature is unimportant when using point cloud deep learning methods for tree species classification. For data collected in a single season, the leaf point cloud has little effect on the classification accuracy. The two suitable point cloud downsampling methods we screened were FPS and NGS, and the deep learning network could provide the most accurate tree species classification when the number of individual tree point clouds was in the range of 2048–5120. Our study further illustrates that point-based end-to-end deep learning methods can be used to classify tree species and identify individual tree point clouds. Combined with the low-cost and high-efficiency BLS system, it can effectively improve the efficiency of forest resource surveys. [ABSTRACT FROM AUTHOR]

A new method for the determination of oxytetracycline (OTC) has been established by coupling the catalytic amplification reaction of copper nanoclusters (CuNCs) with the aptamer reaction. CuNCs prepared by a wet chemical method have the catalytic activity for the formation of gold nanoparticles (AuNPs) resulting from a HAuCl4-ethanol (En) reaction. The experimental results showed that OTC aptamer (Apt) can be adsorbed on the surface of CuNCs in a non-specific way, thus inhibiting its catalytic activity. When OTC was added to the solution, the OTC-Apt complex was generated by a specific reaction, which made the CuNCs desorb and restore their catalytic activity. With the increase of OTC, the recovery of the catalytic activity of CuNCs is strengthened, the reaction speed is accelerated, and the number of AuNPs is increased. The generated AuNPs exhibited surface enhanced Raman scattering (SERS) signals at 1615 cm−1 in the presence of Vitoria blue 4R (VB4R) molecular probes, and a resonance Rayleigh scattering (RRS) peak at 586 nm. There is a good linear relationship between the intensities of SERS, or RRS, and OTC concentration at the range of 37.5–300 ng/L or 37.5–225 ng/L, respectively. A new SERS and RRS assay for the determination of trace OTC based on the regulation of CuNCs catalysis was established. [ABSTRACT FROM AUTHOR]

Bearings-only tracking only adopts measurements from angle sensors to realize target tracking, thus, the accuracy of the state prediction has a significant influence on the final results of filtering. There exist unpredictable approximation errors in the process of filtering due to state propagation, discretization, linearization or other adverse effects. The idea of online covariance adaption is proposed in this work, where the post covariance information is proved to be effective for the covariance adaption. With theoretical deduction, the relationship between the posterior covariance and the priori covariance is investigated; the priori covariance is modified online based on the feedback rule of covariance updating. The general framework integrates the continuous-discrete cubature Kalman filtering and the feedback rule of covariance updating. Numerical results illustrated that the proposed method has advantages over decreasing unpredictable errors and improving the computational accuracy and efficiency. [ABSTRACT FROM AUTHOR]

The leaf area index (LAI) is a crucial parameter for analyzing terrestrial ecosystem carbon cycles and global climate change. Obtaining high spatiotemporal resolution forest stand vegetation LAI products over large areas is essential for an accurate understanding of forest ecosystems. This study takes the northwestern part of the Inner Mongolia Autonomous Region (the northern section of the Greater Khingan Mountains) in northern China as the research area. It also generates the LAI time series product of the 8-day and 30 m forest stand vegetation growth period from 2013 to 2017 (from the 121st to the 305th day of each year). The Simulated Annealing-Back Propagation Neural Network (SA-BPNN) model was used to estimate LAI from Landsat8 OLI, and the multi-period GaoFen-1 WideField-View satellite images (GF-1 WFV) and the spatiotemporal adaptive reflectance fusion mode (STARFM) was used to predict high spatiotemporal resolution LAI by combining inversion LAI and Global LAnd Surface Satellite-derived vegetation LAI (GLASS LAI) products. The results showed the following: (1) The SA-BPNN estimation model has relatively high accuracy, with R2 = 0.75 and RMSE = 0.38 for the 2013 LAI estimation model, and R2 = 0.74 and RMSE = 0.17 for the 2016 LAI estimation model. (2) The fused 30 m LAI product has a good correlation with the LAI verification of the measured sample site (R2 = 0.8775) and a high similarity with the GLASS LAI product. (3) The fused 30 m LAI product has a high similarity with the GLASS LAI product, and compared with the GLASS LAI interannual trend line, it accords with the growth trend of plants in the seasons. This study provides a theoretical and technical reference for forest stand vegetation growth period LAI spatiotemporal fusion research based on high-score data, and has an important role in exploring vegetation primary productivity and carbon cycle changes in the future. [ABSTRACT FROM AUTHOR]

Tree species information is an important factor in forest resource surveys, and light detection and ranging (LiDAR), as a new technical tool for forest resource surveys, can quickly obtain the 3D structural information of trees. In particular, the rapid and accurate classification and identification of tree species information from individual tree point clouds using deep learning methods is a new development direction for LiDAR technology in forest applications. In this study, mobile laser scanning (MLS) data collected in the field are first pre-processed to extract individual tree point clouds. Two downsampling methods, non-uniform grid and farthest point sampling, are combined to process the point cloud data, and the obtained sample data are more conducive to the deep learning model for extracting classification features. Finally, four different types of point cloud deep learning models, including pointwise multi-layer perceptron (MLP) (PointNet, PointNet++, PointMLP), convolution-based (PointConv), graph-based (DGCNN), and attention-based (PCT) models, are used to classify and identify the individual tree point clouds of eight tree species. The results show that the classification accuracy of all models (except for PointNet) exceeded 0.90, where the PointConv model achieved the highest classification accuracy for tree species classification. The streamlined PointMLP model can still achieve high classification accuracy, while the PCT model did not achieve good accuracy in the tree species classification experiment, likely due to the small sample size. We compare the training process and final classification accuracy of the different types of point cloud deep learning models in tree species classification experiments, further demonstrating the advantages of deep learning techniques in tree species recognition and providing experimental reference for related research and technological development. [ABSTRACT FROM AUTHOR]

Forest carbon flux is critical to climate change, and the accurate modeling of forest carbon flux is an extremely challenging task. The remote sensing model (the MODIS MOD_17 gross primary productivity (GPP) model (MOD_17)) has strong practicability and is widely used around the world. The ecological process (the Biome-BioGeochemical Cycles Multilayer Soil Module model (Biome-BGCMuSo)) model can describe most of the vegetation's environmental and physiological processes on fine time scales. Nevertheless, complex parameters and calibrations pose challenges to the application and development of models. In this study, we optimized all the input parameters of the MOD_17 model for the calibration of the Biome-BGCMuSo model to obtain GPP with continuous spatiality. To determine the contribution of input parameters to the GPP of different forest types, an Extended Fourier Amplitude Sensitivity Test (EFAST) was performed on the Biome-BGCMuSo model firstly. Then, we selected the sample points of each forest type and its different ecological gradients (30 for each type), using the GPP simulation value of the optimized MOD_17 model corresponding to the time and space scale to calibrate the Biome-BGCMuSo model, to drive the calibrated Biome-BGCMuSo, and we simulated the different forest types' net primary productivity (NPP). According to dendrochronological measurements, the NPP simulation results were verified on the whole regional scale. The results showed that the GPP values of different forest types were highly sensitive to C:Nleaf (C:N of leaf), SLA1 (canopy average specific leaf area in phenological phase 1), and FLNR (fraction of leaf N in Rubisco). The coefficient of determination (R2) between the simulated forest NPP and the measured NPP was 0.64, and the root-mean-square (RMSE) was 26.55 g/C/m2/year. Our study aims to reduce uncertainty in forest carbon fluxes simulated by the Biome-BGCMuSo model, providing feedback for understanding forest ecosystem carbon cycling, vegetation productivity, and climate change. [ABSTRACT FROM AUTHOR]