1. The dichloromethane fraction from Mahonia bealei (Fort.) Carr. leaves exerts an anti-inflammatory effect both in vitro and in vivo.
- Author
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Hu W, Wu L, Qiang Q, Ji L, Wang X, Luo H, Wu H, Jiang Y, Wang G, and Shen T
- Subjects
- Animals, Anti-Inflammatory Agents isolation & purification, Cytokines metabolism, Disease Models, Animal, Dose-Response Relationship, Drug, Inflammation Mediators metabolism, Lipopolysaccharides pharmacology, Lung immunology, Lung metabolism, Macrophage Activation drug effects, Macrophages, Peritoneal immunology, Macrophages, Peritoneal metabolism, Male, Mice, Mice, Inbred C57BL, NF-kappa B metabolism, Phosphatidylinositol 3-Kinase metabolism, Phytotherapy, Plant Extracts isolation & purification, Plants, Medicinal, Pneumonia chemically induced, Pneumonia immunology, Pneumonia metabolism, Proto-Oncogene Proteins c-akt metabolism, RAW 264.7 Cells, Signal Transduction drug effects, Time Factors, Anti-Inflammatory Agents pharmacology, Lung drug effects, Macrophages, Peritoneal drug effects, Mahonia chemistry, Methylene Chloride chemistry, Plant Extracts pharmacology, Plant Leaves chemistry, Pneumonia prevention & control, Solvents chemistry
- Abstract
Ethnopharmacological Relevance: Mahonia bealei has a long history of medical use in traditional Chinese medicine for the treatment of inflammatory-associated diseases. Despite numerous phytochemical and pharmacological studies, there is a lack of systematic studies to understand the cellular and molecular mechanisms of the anti-inflammatory activity of this plant., Aim of Study: This study aimed to evaluate the anti-inflammatory activity of the dichloromethane fraction from M. bealei leaves (MBL-CH)., Materials and Methods: RAW 264.7 cells were pretreated with different concentrations of MBL-CH for 30min prior to treatment with 1μg/ml of lipopolysaccharide (LPS). The nuclear factor κB (NF-κB) pathway and subsequent production of inflammatory mediators, such as nitric oxide (NO), prostaglandin E2 (PGE2), and tumour necrosis factor (TNF)-α were investigated. Furthermore, the in vivo mouse model of LPS-induced acute lung injury (ALI) was employed to study the anti-inflammatory effects of MBL-CH., Results: Pre-treatment with MBL-CH significantly inhibited the LPS-stimulated secretion of NO, PGE2, and TNF-α into the culture medium, as well as the mRNA levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-α, which were associated with a reduction in the phosphorylation of IκBα, Akt, and PI3K and inhibition of the transcriptional activity of NF-κB. Furthermore, in vivo experiments revealed that MBL-CH attenuated LPS-stimulated lung inflammation in mice., Conclusion: Taken together, our findings indicate that MBL-CH attenuates LPS-stimulated inflammatory responses in macrophages by blocking NF-κB activation through interference with activation of the PI3K/Akt pathway, providing scientific evidence that the plant can be employed in traditional remedies., (Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2016
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