Fishbein, Eugenia, Alcocer-Varela, J., and Alarcón-Segovia, D.
Subjects
T cells, IMMUNOGLOBULINS, INTERLEUKIN-2, MONOCLONAL antibodies, LYMPHOCYTES, INTERLEUKINS, SCIENTIFIC experimentation
Abstract
In this paper we present experiments that indicate that, in man, most T-celI subpopulations produce interleukin-2 (IL-2), but that the main cell subpopulation which produces it, both upon activation with phytohaemagglutinin or in autologous mixed lymphocyte cultures, is that of autologous rosette-forming (Tar) T4+ T cells. Conversely, the main IL-2-responding T-cell subpopulation is that composed of T cells depleted of Tar (T-Tar) that are T8+ IL-2 was also found to be more effectively produced by Tar cells that do not bind peanut agglutinin (PNA) than by those that do. The PNA T4+ Tar cells were also found to respond best to interleukin- I (IL-I). [ABSTRACT FROM AUTHOR]
Oravecz, T., Monostori, É., Kurucz, É., Takács, L., and Andó, I.
Subjects
EPITOPES, INTERLEUKINS, T cells, MONOCLONAL antibodies, IMMUNOGLOBULINS, LYMPHOCYTES
Abstract
In the present study we have investigated the effect of CD45, CD45RA and CD45RO monoclonal antibodies (MoAbs) on the CD3 receptor-mediated proliferation of human T lymphocytes, It is shown that CD3-induced proliferation of purified resting T cells and quiescent T lymphoblasts (QTL) is promoted via all of the investigated CD45-associatcd epitopes. lt is also shown that the CD45 molecules are required to be cross-linked for costimulation. The MoAbs enhance the jnterleukin-2 (IL-2) production of CD3-stimulaled QTL, The elevation of the IL-2 production correlates with the increase in CD3-induced cell proliferation suggesting that the CD45-driven regulation of T lymphocyte activation is linked to the IL-2 pathway. [ABSTRACT FROM AUTHOR]