20 results on '"Peng, Xiuhua"'
Search Results
2. Chinese herbal medicine mixture 919 syrup alleviates nonalcoholic fatty liver disease in rats by inhibiting the NF-κB pathway
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Chen, Manman, Xing, Jingwei, Pan, Danqing, Peng, Xiuhua, and Gao, Pengfei
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- 2020
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3. Effectiveness of dyadic interventions among cancer dyads: An overview of systematic reviews and meta‐analyses.
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Sun, Qian, Wang, Kunyuan, Chen, Yingliang, Peng, Xiuhua, Jiang, Xiaohan, and Peng, Junsheng
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WELL-being ,CAREGIVER attitudes ,ONLINE information services ,MEDICAL databases ,CINAHL database ,MEDICAL information storage & retrieval systems ,CONFIDENCE intervals ,SYSTEMATIC reviews ,HEALTH status indicators ,CANCER patients ,COMPARATIVE studies ,DESCRIPTIVE statistics ,MEDLINE ,INFORMATION storage & retrieval systems - Abstract
Aims and Objective: To summarize evidence from systematic reviews (SRs)/meta‐analyses (MAs) regarding the impact of dyadic interventions delivered to both members of a cancer dyad, including a cancer patient and caregiver (e.g. family caregiver, intimate partner). Design: This overview of SRs was conducted in accordance with the preferred reporting items for overviews of reviews statement. Methods: A comprehensive search of multiple databases, including PubMed, Cochrane Library, Embase, CINAHL, Web of Science, China National Knowledge Infrastructure and Wan Fang. The methodological and reporting quality of SRs and MAs was assessed using the Assessing the Methodological Quality of Systematic Reviews 2. The quality of the included SRs/MAs was evaluated using the Grades of Recommendations, Assessment, Development and Evaluation approach. Results: Eighteen SRs/MAs undertook quantitative synthesis to assess the impact of dyadic interventions on cancer dyads. Both the credibility of the SRs/MAs and the evidence quality of the outcome measures were below satisfactory standards. Prior SRs/MAs revealed several limitations such as lack of pre‐published protocols or research objectives, failure to report excluded studies and insufficient details on funding sources for individual studies. Conclusions: Dyadic interventions may prove advantageous for the physical health and dyadic adjustment of cancer dyads. Nevertheless, the reported results of dyadic interventions on the psychological health of patient–caregiver dyads affected by cancer are inconsistent. Thus, rigorous and comprehensive studies are requisite to establish reliable evidence for conclusive determinations. Relevance to Clinical Practice: The findings of this overview can guide healthcare practitioners when considering the use of dyadic interventions for cancer dyads. Moreover, these findings have the potential to enhance the integration of these approaches into clinical practice. Patient or Public Contribution: Our paper presents an overview of systematic reviews, and therefore, such specific details may not be relevant to our study. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Aldehyde dehydrogenase 2 deficiency promotes atherosclerotic plaque instability through accelerating mitochondrial ROS-mediated vascular smooth muscle cell senescence
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Zhu, Hong, Wang, Zeng, Dong, Zhen, Wang, Cong, Cao, Quan, Fan, Fan, Zhao, Jingjing, Liu, Xiangwei, Yuan, Meng, Sun, Xiaolei, Peng, Xiuhua, Zou, Yunzeng, Zhou, Jingmin, Ge, Junbo, Zhou, Xiaohui, and Zhang, Yingmei
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- 2019
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5. Characterization of genetic humanized mice with transgenic HLA DP401 or DRA but deficient in endogenous murine MHC class II genes upon Staphylococcus aureus pneumonia.
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Li, Feng, Niu, Bowen, Liu, Lingling, Zhu, Mengmin, Yang, Hua, Qin, Boyin, Peng, Xiuhua, Chen, Lixiang, Xu, Chunhua, and Zhou, Xiaohui
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- 2023
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6. Generation and expression analysis of BAC humanized mice carrying HLA‐DP401 haplotype
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Feng Li, Hua Yang, Meixiang Wang, Ling‐ling Liu, Xiaonan Ren, Boyin Qin, Xiaohui Zhou, Mengmin Zhu, Bowen Niu, and Peng Xiuhua
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Genetically modified mouse ,Chromosomes, Artificial, Bacterial ,HLA-DP Antigens ,Medicine (General) ,Transgene ,Clone (cell biology) ,HLA‐DP4 ,Human leukocyte antigen ,bacterial artificial chromosome (BAC) ,Biology ,Mice ,R5-920 ,Antigen ,HLA-DQ Antigens ,Gene expression ,Animals ,Gene ,Bacterial artificial chromosome ,General Medicine ,Original Articles ,Molecular biology ,Mice, Inbred C57BL ,humanized mice ,Haplotypes ,gene expression ,Original Article ,Staphylococcus aureus pneumonia - Abstract
Background Human leukocyte antigen (HLA)‐DP is much less studied than other HLA class II antigens, that is, HLA‐DR and HLA‐DQ, etc. However, the accumulating data have suggested the important roles of DP‐restricted responses in the context of cancer, allergy, and infectious disease. Lack of animal models expressing these genes as authentic cis‐haplotypes blocks our understanding for the role of HLA‐DP haplotypes in immunity. Methods To explore the potential cis‐acting control elements involved in the transcriptional regulation of the HLA‐DPA1/DPB1 gene, we performed the expression analysis using bacterial artificial chromosome (BAC)‐based transgenic humanized mice in the C57BL/6 background, which carried the entire HLA‐DP401 gene locus. We further developed a mouse model of Staphylococcus aureus pneumonia in HLA‐DP401 humanized transgenic mice, and performed the analysis on the expression pattern of HLA‐DP401 and immunological responses in the model. Results In this study, we screened and identified a BAC clone spanning the entire HLA‐DP gene locus. DNA from this clone was analyzed for integrity by pulsed‐field gel electrophoresis and then microinjected into fertilized mouse oocytes to produce transgenic founder animals. Nine sets of PCR primers for regional markers with an average distance of 15 kb between each primer were used to confirm the integrity of the transgene in the five transgenic lines carrying the HLA‐DPA1/DPB1 gene. Transgene copy numbers were determined by real‐time PCR analysis. HLA‐DP401 gene expression was analyzed at the mRNA and protein level. Although infection with S aureus Newman did not alter the percentage of immune cells in the spleen and thymus from the HLA‐DP401‐H2‐Aβ1 humanized mice. Increased expression of HLA‐DP401 was observed in the thymus of the humanized mice infected by S aureus. Conclusions We generated several BAC transgenic mice, and analyzed the expression of HLA‐DPA1/DPB1 in those mice. A model of S aureus‐induced pneumonia in the HLA‐DP401‐H2‐Aβ1−/− humanized mice was further developed, and S aureus infection upregulated the HLA‐DP401 expression in thymus of those humanized mice. These findings demonstrate the potential of those HLA‐DPA1/DPB1 transgenic humanized mice for developing animal models of infectious diseases and MHC‐associated immunological diseases.
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- 2021
7. An Efficient Antiviral Strategy for Targeting Hepatitis B Virus Genome Using Transcription Activator-Like Effector Nucleases
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Chen, Jieliang, Zhang, Wen, Lin, Junyu, Wang, Fan, Wu, Min, Chen, Cuncun, Zheng, Ye, Peng, Xiuhua, Li, Jianhua, and Yuan, Zhenghong
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- 2014
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8. Gene expression profile in peripheral blood mononuclear cells of postpartum depression patients
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Pan, Danqing, Xu, Yuemei, Zhang, Lei, Su, Qizhu, Chen, Manman, Li, Bing, Xiao, Qian, Gao, Qi, Peng, Xiuhua, Jiang, Binfei, Gu, Yilu, Du, Yuling, and Gao, Pengfei
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- 2018
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9. Epigenetic Landscapes of Single-Cell Chromatin Accessibility and Transcriptomic Immune Profiles of T Cells in COVID-19 Patients
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Yun Ling, Shun Li, Feng Li, Ling‐ling Liu, Boyin Qin, Yang Liu, Tongyu Zhu, Zhaoqin Zhu, Mengmin Zhu, Lixiang Chen, Yixin Liao, Hua Yang, Peng Xiuhua, Chunhua Xu, Yinzhong Shen, Bowen Niu, Zhenyan Wang, Xiaonan Ren, Bin Wu, Hongzhou Lu, Jun Chen, Mingquan Guo, Chao Wang, Li Liu, and Xiaohui Zhou
- Subjects
0301 basic medicine ,CD4-Positive T-Lymphocytes ,lcsh:Immunologic diseases. Allergy ,ScRNA-seq ,Immunology ,T cells ,Transposases ,Biology ,CD8-Positive T-Lymphocytes ,Lymphocyte Activation ,Transcriptome ,03 medical and health sciences ,Epigenome ,0302 clinical medicine ,Immune system ,Single-cell analysis ,NF-KappaB Inhibitor alpha ,Immunology and Allergy ,Calgranulin B ,Humans ,scATAC-seq ,030212 general & internal medicine ,Epigenetics ,Original Research ,Inflammation ,Immunity, Cellular ,Sequence Analysis, RNA ,SARS-CoV-2 ,Gene Expression Profiling ,COVID-19 ,transcriptome profiling ,Molecular biology ,Chromatin ,Up-Regulation ,Gene expression profiling ,Class Ia Phosphatidylinositol 3-Kinase ,030104 developmental biology ,chromatin accessibility ,Tumor necrosis factor alpha ,Single-Cell Analysis ,lcsh:RC581-607 ,CD8 - Abstract
T cells play a critical role in coronavirus diseases. How they do so in COVID-19 may be revealed by analyzing the epigenetic chromatin accessibility of cis- and trans-regulatory elements and creating transcriptomic immune profiles. We performed single-cell assay for transposase-accessible chromatin (scATAC) and single-cell RNA (scRNA) sequencing (seq) on the peripheral blood mononuclear cells (PBMCs) of severely ill/critical patients (SCPs) infected with COVID-19, moderate patients (MPs), and healthy volunteer controls (HCs). About 76,570 and 107,862 single cells were used, respectively, for analyzing the characteristics of chromatin accessibility and transcriptomic immune profiles by the application of scATAC-seq (nine cases) and scRNA-seq (15 cases). The scATAC-seq detected 28,535 different peaks in the three groups; among these peaks, 41.6 and 10.7% were located in the promoter and enhancer regions, respectively. Compared to HCs, among the peak-located genes in the total T cells and its subsets, CD4+ T and CD8+ T cells, from SCPs and MPs were enriched with inflammatory pathways, such as mitogen-activated protein kinase (MAPK) signaling pathway and tumor necrosis factor (TNF) signaling pathway. The motifs of TBX21 were less accessible in the CD4+ T cells of SCPs compared with those in MPs. Furthermore, the scRNA-seq showed that the proportion of T cells, especially the CD4+ T cells, was decreased in SCPs and MPs compared with those in HCs. Transcriptomic results revealed that histone-related genes, and inflammatory genes, such as NFKBIA, S100A9, and PIK3R1, were highly expressed in the total T cells, CD4+ T and CD8+ T cells, both in the cases of SCPs and MPs. In the CD4+ T cells, decreased T helper-1 (Th1) cells were observed in SCPs and MPs. In the CD8+T cells, activation markers, such as CD69 and HLA class II genes (HLA-DRA, HLA-DRB1, and HLA-DRB5), were significantly upregulated in SCPs. An integrated analysis of the data from scATAC-seq and scRNA-seq showed some consistency between the approaches. Cumulatively, we have generated a landscape of chromatin epigenetic status and transcriptomic immune profiles of T cells in patients with COVID-19. This has provided a deeper dissection of the characteristics of the T cells involved at a higher resolution than from previously obtained data merely by the scRNA-seq analysis. Our data led us to suggest that the T-cell inflammatory states accompanied with defective functions in the CD4+ T cells of SCPs may be the key factors for determining the pathogenesis of and recovery from COVID-19.
- Published
- 2021
10. Epigenetic Landscapes of Single-Cell Chromatin Accessibility and Transcriptomic Immune Profiles of T Cells in COVID-19 Patients.
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Li, Shun, Wu, Bin, Ling, Yun, Guo, Mingquan, Qin, Boyin, Ren, Xiaonan, Wang, Chao, Yang, Hua, Chen, Lixiang, Liao, Yixin, Liu, Yang, Peng, Xiuhua, Xu, Chunhua, Wang, Zhenyan, Shen, Yinzhong, Chen, Jun, Liu, Li, Niu, Bowen, Zhu, Mengmin, and Liu, Lingling
- Subjects
T cells ,COVID-19 ,MITOGEN-activated protein kinases ,TUMOR necrosis factors ,CHROMATIN - Abstract
T cells play a critical role in coronavirus diseases. How they do so in COVID-19 may be revealed by analyzing the epigenetic chromatin accessibility of cis- and trans-regulatory elements and creating transcriptomic immune profiles. We performed single-cell assay for transposase-accessible chromatin (scATAC) and single-cell RNA (scRNA) sequencing (seq) on the peripheral blood mononuclear cells (PBMCs) of severely ill/critical patients (SCPs) infected with COVID-19, moderate patients (MPs), and healthy volunteer controls (HCs). About 76,570 and 107,862 single cells were used, respectively, for analyzing the characteristics of chromatin accessibility and transcriptomic immune profiles by the application of scATAC-seq (nine cases) and scRNA-seq (15 cases). The scATAC-seq detected 28,535 different peaks in the three groups; among these peaks, 41.6 and 10.7% were located in the promoter and enhancer regions, respectively. Compared to HCs, among the peak-located genes in the total T cells and its subsets, CD4
+ T and CD8+ T cells, from SCPs and MPs were enriched with inflammatory pathways, such as mitogen-activated protein kinase (MAPK) signaling pathway and tumor necrosis factor (TNF) signaling pathway. The motifs of TBX21 were less accessible in the CD4+ T cells of SCPs compared with those in MPs. Furthermore, the scRNA-seq showed that the proportion of T cells, especially the CD4+ T cells, was decreased in SCPs and MPs compared with those in HCs. Transcriptomic results revealed that histone-related genes, and inflammatory genes, such as NFKBIA, S100A9, and PIK3R1, were highly expressed in the total T cells, CD4+ T and CD8+ T cells, both in the cases of SCPs and MPs. In the CD4+ T cells, decreased T helper-1 (Th1) cells were observed in SCPs and MPs. In the CD8+ T cells, activation markers, such as CD69 and HLA class II genes (HLA-DRA, HLA-DRB1, and HLA-DRB5), were significantly upregulated in SCPs. An integrated analysis of the data from scATAC-seq and scRNA-seq showed some consistency between the approaches. Cumulatively, we have generated a landscape of chromatin epigenetic status and transcriptomic immune profiles of T cells in patients with COVID-19. This has provided a deeper dissection of the characteristics of the T cells involved at a higher resolution than from previously obtained data merely by the scRNA-seq analysis. Our data led us to suggest that the T-cell inflammatory states accompanied with defective functions in the CD4+ T cells of SCPs may be the key factors for determining the pathogenesis of and recovery from COVID-19. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
11. Effect of immobilization stress on the appetite and stomach ghrelin expression in maternal mice
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Li, Bing, Xu, Yuemei, Pan, Danqing, Xiao, Qian, Gao, Qi, Chen, Xin, Peng, Xiuhua, Du, Yuling, and Gao, Pengfei
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Leptin ,Restraint, Physical ,Pro-Opiomelanocortin ,Time Factors ,Corticotropin-Releasing Hormone ,Hypothalamus ,Weight Gain ,Eating ,Receptor, Serotonin, 5-HT2B ,Receptor, Serotonin, 5-HT2C ,Animals ,Lactation ,Neuropeptide Y ,Receptors, Ghrelin ,Mice, Inbred ICR ,Appetite Regulation ,digestive, oral, and skin physiology ,Postpartum Period ,Feeding Behavior ,Ghrelin ,Disease Models, Animal ,Gastric Mucosa ,Maternal Exposure ,Agouti Signaling Protein ,Original Article ,Female ,hormones, hormone substitutes, and hormone antagonists ,Stress, Psychological - Abstract
Maternal stress exerts long-lasting postnatal growth on offspring, which persist into adulthood. However, the effect of maternal stress on appetizing system has not been widely reported. In this study, we found that maternal immobilization stress (IS) during lactation resulted in low body weight and food intake. Immunohistochemistry showed an increase in stomach ghrelin protein expression. The central regulation of body weight and food intake occurs in the hypothalamus, which contains multiple neuronal systems that play important roles in the regulation of energy homeostasis. These systems including multiple neuropeptides involve in the ghrelin pathway of appetite regulation. Therefore, real time reverse transcription polymerase chain reaction (RT-PCR) was used to measure the change of mRNA expression of ghrelin pathway related hormones in order to explore the mechanisms involved in the appetite regulation. Expression levels of the hypothalamic 5-hydroxytryptamine 2c receptor (5-HT2cR) and 5-HT2bR, which are essential for the development and function of ghrelin and leptin, were decreased, as well as those of corticotrophin releasing factor (CRF) and pro-opiomelanocortin (POMC). While the expression of growth hormone secretagogue receptor (GHSR), neuropeptide-Y (NPY) and agouti-related protein (AgRP) showed an increase with significant difference. These results suggest that stress in a postpartum mother has persistent effects on the body weight of their offspring. Increased ghrelin and decreased leptin expression in the stomach may play a role in these effects.
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- 2015
12. Oral Administered Particulate Yeast-Derived Glucan Promotes Hepatitis B Virus Clearance in a Hydrodynamic Injection Mouse Model
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Xiaoyu Yu, Xiaohui Zhou, Min Wu, Peng Xiuhua, Dandan Zhang, Xiaonan Zhang, Cong Wang, Zhenghong Yuan, Guangxu Ren, Bisheng Shi, Maya Kozlowski, and Zhong Fang
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Hepatitis B virus ,T cell ,lcsh:Medicine ,Saccharomyces cerevisiae ,Biology ,Adaptive Immunity ,medicine.disease_cause ,Virus Replication ,Virus ,Injections ,Mice ,Immune system ,Hepatitis B, Chronic ,Interstitial fluid ,T-Lymphocyte Subsets ,medicine ,Animals ,Humans ,Immunologic Factors ,lcsh:Science ,Glucans ,Multidisciplinary ,Macrophages ,lcsh:R ,Transfection ,Dendritic Cells ,Acquired immune system ,Disease Models, Animal ,medicine.anatomical_structure ,Viral replication ,Liver ,Immunology ,lcsh:Q ,Research Article - Abstract
Hepatitis B virus (HBV) persistent infection is associated with ineffective immune response for the clearance of virus. Immunomodulators represent an important class of therapeutics, which potentially could be beneficial for the treatment of HBV infection. The particulate yeast-derived glucan (PYDG) has been shown to enhance the innate and adaptive immune responses. We therefore, assessed the efficacy of PYDG in enhancing HBV specific immune responses by employing the hydrodynamic injection-based (HDI) HBV transfection mouse model. Mice were intragatric administered PYDG daily for 9 weeks post pAAV/HBV1.2 hydrodynamic injection. PYDG treatment significantly promoted HBV DNA clearance and production of HBsAb compared to control mice. PYDG treatment resulted in recruitment of macrophages, dendritic cells (DCs) and effector T cells to the liver microenvironment, accompanied by a significantly augmented DCs maturation and HBV-specific IFN-γ and TNF-α production by T cell. In addition, enhanced production of Th1 cytokines in liver tissue interstitial fluid (TIF) was associated with PYDG administration. Live imaging showed the accumulation of PYDG in the mouse liver. Our results demonstrate that PYDG treatment significantly enhances HBV-specific Th1 immune responses, accompanied by clearance of HBV DNA, and therefore holds promise for further development of therapeutics against chronic hepatitis B.
- Published
- 2015
13. An Efficient Antiviral Strategy for Targeting Hepatitis B Virus Genome Using Transcription Activator-Like Effector Nucleases
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Fan Wang, Wen Zhang, Jianhua Li, Min Wu, Zhenghong Yuan, Peng Xiuhua, Jieliang Chen, Junyu Lin, Cuncun Chen, and Ye Zheng
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HBsAg ,Hepatitis B virus ,Genetic Vectors ,Genome, Viral ,Biology ,medicine.disease_cause ,Antiviral Agents ,Virus ,Cell Line ,chemistry.chemical_compound ,Mice ,Viral Proteins ,Hepatitis B, Chronic ,Drug Discovery ,Genetics ,medicine ,Animals ,Humans ,Molecular Biology ,Pharmacology ,Transcription activator-like effector nuclease ,Deoxyribonucleases ,virus diseases ,Interferon-alpha ,cccDNA ,Virology ,Molecular biology ,digestive system diseases ,HBcAg ,Disease Models, Animal ,HBeAg ,chemistry ,Molecular Medicine ,Original Article ,DNA, Circular ,DNA ,Plasmids ,Protein Binding - Abstract
The hepatitis B virus (HBV) is a DNA virus that can cause chronic hepatitis B (CHB) in humans. Current therapies for CHB infection are limited in efficacy and do not target the pre-existing viral genomic DNA, which are present in the nucleus as a covalently closed circular DNA (cccDNA) form. The transcription activator-like (TAL) effector nucleases (TALENs) are newly developed enzymes that can cleave sequence-specific DNA targets. Here, TALENs targeting the conserved regions of the viral genomic DNA among different HBV genotypes were constructed. The expression of TALENs in Huh7 cells transfected with monomeric linear full-length HBV DNA significantly reduced the viral production of HBeAg, HBsAg, HBcAg, and pgRNA, resulted in a decreased cccDNA level and misrepaired cccDNAs without apparent cytotoxic effects. The anti-HBV effect of TALENs was further demonstrated in a hydrodynamic injection-based mouse model. In addition, an enhanced antiviral effect with combinations of TALENs and interferon-α (IFN-α) treatment was observed and expression of TALENs restored HBV suppressed IFN-stimulated response element-directed transcription. Taken together, these data indicate that TALENs can specifically target and successfully inactivate the HBV genome and are potently synergistic with IFN-α, thus providing a potential therapeutic strategy for treating CHB infection.
- Published
- 2013
14. Oral Administered Particulate Yeast-Derived Glucan Promotes Hepatitis B Virus Clearance in a Hydrodynamic Injection Mouse Model.
- Author
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Yu, Xiaoyu, Zhang, Dandan, Shi, Bisheng, Ren, Guangxu, Peng, Xiuhua, Fang, Zhong, Kozlowski, Maya, Zhou, Xiaohui, Zhang, Xiaonan, Wu, Min, Wang, Cong, and Yuan, Zhenghong
- Subjects
HEPATITIS B treatment ,GENE transfection ,IMMUNE response ,GLUCANS ,LABORATORY mice ,HYDRODYNAMICS - Abstract
Hepatitis B virus (HBV) persistent infection is associated with ineffective immune response for the clearance of virus. Immunomodulators represent an important class of therapeutics, which potentially could be beneficial for the treatment of HBV infection. The particulate yeast-derived glucan (PYDG) has been shown to enhance the innate and adaptive immune responses. We therefore, assessed the efficacy of PYDG in enhancing HBV specific immune responses by employing the hydrodynamic injection-based (HDI) HBV transfection mouse model. Mice were intragatric administered PYDG daily for 9 weeks post pAAV/HBV1.2 hydrodynamic injection. PYDG treatment significantly promoted HBV DNA clearance and production of HBsAb compared to control mice. PYDG treatment resulted in recruitment of macrophages, dendritic cells (DCs) and effector T cells to the liver microenvironment, accompanied by a significantly augmented DCs maturation and HBV-specific IFN-γ and TNF-α production by T cell. In addition, enhanced production of Th1 cytokines in liver tissue interstitial fluid (TIF) was associated with PYDG administration. Live imaging showed the accumulation of PYDG in the mouse liver. Our results demonstrate that PYDG treatment significantly enhances HBV-specific Th1 immune responses, accompanied by clearance of HBV DNA, and therefore holds promise for further development of therapeutics against chronic hepatitis B. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
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15. Influence on fishes of dissolved gas supersaturation caused by high-dam discharging and its countermeasures.
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Wang Qing, Dai Huichao, Peng Xiuhua, and Wang Yu
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- 2011
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16. Monocytic MDSCs homing to thymus contribute to age-related CD8+ T cell tolerance of HBV.
- Author
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Fang Z, Zhang Y, Zhu Z, Wang C, Hu Y, Peng X, Zhang D, Zhao J, Shi B, Shen Z, Wu M, Xu C, Chen J, Zhou X, Xie Y, Yu H, Zhang X, Li J, Hu Y, Kozlowski M, Bertoletti A, and Yuan Z
- Subjects
- Animals, CD8-Positive T-Lymphocytes, Hepatitis B Surface Antigens, Hepatitis B virus, Humans, Mice, Hepatitis B, Chronic, Myeloid-Derived Suppressor Cells
- Abstract
Hepatitis B virus exposure in children usually develops into chronic hepatitis B (CHB). Although hepatitis B surface antigen (HBsAg)-specific CD8+ T cells contribute to resolve HBV infection, they are preferentially undetected in CHB patients. Moreover, the mechanism for this rarely detected HBsAg-specific CD8+ T cells remains unexplored. We herein found that the frequency of HBsAg-specific CD8+ T cells was inversely correlated with expansion of monocytic myeloid-derived suppressor cells (mMDSCs) in young rather than in adult CHB patients, and CCR9 was upregulated by HBsAg on mMDSCs via activation of ERK1/2 and IL-6. Sequentially, the interaction between CCL25 and CCR9 mediated thymic homing of mMDSCs, which caused the cross-presentation, transferring of peripheral HBsAg into the thymic medulla, and then promoted death of HBsAg-specific CD8+ thymocytes. In mice, adoptive transfer of mMDSCs selectively obliterated HBsAg-specific CD8+ T cells and facilitated persistence of HBV in a CCR9-dependent manner. Taken together, our results uncovered a novel mechanism for establishing specific CD8+ tolerance to HBsAg in chronic HBV infection., (© 2022 Fang et al.)
- Published
- 2022
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17. Image-localized body surface marking for the intraoperative localization of pulmonary ground-glass nodules.
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Xu P, Peng X, Li W, and Yu H
- Abstract
Background: The method of locating pulmonary nodules before operation plays a crucial role in the surgery of pulmonary ground-glass nodules (GGNs). However, the methodologies surrounding intraoperative localization remains limited, with the majority procedures requiring specific additional equipment. We report a new approach in locating pulmonary GGNs by image-localized body surface marking intraoperative (IBMI) localization., Methods: A retrospective review of the medical records of 76 patients with pulmonary GGNs was performed. All patients underwent IBMI localization between January 2018 and March 2019. Twenty-six patients underwent CT-guided hook wire localization before IBMI localization during surgery. IBMI localization was undertaken directly without pre-treatment in the remaining patients. The efficacy and complications of this approach were analyzed and compared with other pre- or intraoperative localization methods in the current literature., Results: The intraoperative localizations were performed successfully in 72 of all 76 patients pulmonary GGNs within a mean duration of 5.3±1.8 (range, 2.0 to 9.6) minutes. The GGNs in four cases were found to have a significant deviation (>1.5 cm) from the positioning points. All GGNs were successfully resected. Except for five cases of active chest wall bleeding (6.5%), no other intra- or postoperative complications occurred., Conclusions: The IBMI localization approach is a safe and short-duration procedure with high success rates and fewer complications. We used it for the first time for intraoperative localization of peripheral GGNs with excellent results., Competing Interests: Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at http://dx.doi.org/10.21037/qims-19-947). The authors have no conflicts of interest to declare., (2020 Quantitative Imaging in Medicine and Surgery. All rights reserved.)
- Published
- 2020
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18. Hepatomas are exquisitely sensitive to pharmacologic ascorbate (P-AscH - ).
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Zhang X, Liu T, Li Z, Feng Y, Corpe C, Liu S, Zhang J, He X, Liu F, Xu L, Shen L, Li S, Xia Q, Peng X, Zhou X, Chen W, Zhang X, Xu J, and Wang J
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- Animals, Antineoplastic Agents pharmacology, Apoptosis drug effects, Cell Death drug effects, Cell Line, Cell Proliferation drug effects, Disease Models, Animal, Gene Expression Regulation, Neoplastic drug effects, Gene Expression Regulation, Neoplastic genetics, Liver Neoplasms drug therapy, Mice, Oxidative Stress drug effects, Oxidative Stress genetics, Reactive Oxygen Species, Signal Transduction drug effects, Signal Transduction genetics, Ascorbic Acid pharmacology, Carcinoma, Hepatocellular drug therapy, Cell Line, Tumor drug effects
- Abstract
Rationale: Ascorbate is an essential micronutrient known for redox functions at normal physiologic concentrations. In recent decades, pharmacological ascorbate has been found to selectively kill tumour cells. However, the dosing frequency of pharmacologic ascorbate in humans has not yet been defined. Methods: We determined that among five hepatic cell lines, Huh-7 cells were the most sensitive to ascorbate. The effects of high-dose ascorbate on hepatoma were therefore assessed using Huh-7 cells and xenograft tumour mouse model. Results: In Huh-7 cells, ascorbate induced a significant increase in the percentage of cells in the G0/G1 phase, apoptosis and intracellular levels of ROS. High doses of ascorbate (4.0 pmol cell
-1 ), but not low doses of ascorbate (1.0 pmol cell-1 ), also served as a pro-drug that killed hepatoma cells by altering mitochondrial respiration. Furthermore, in a Huh-7 cell xenograft tumour mouse model, intraperitoneal injection of ascorbate (4.0 g/kg/3 days) but not a lower dose of ascorbate (2.0 g/kg/3 days) significantly inhibited tumour growth. Gene array analysis of HCC tumour tissue from xenograft mice given IP ascorbate (4.0 g/kg/3 days) identified changes in the transcript levels of 192 genes/ncRNAs involved in insulin receptor signalling, metabolism and mitochondrial respiration. Consistent with the array data, gene expression levels of AGER, DGKK, ASB2, TCP10L2, Lnc-ALCAM-3 , and Lnc-TGFBR2-1 were increased 2.05-11.35 fold in HCC tumour tissue samples from mice treated with high-dose ascorbate, and IHC staining analysis also verified that AGER/RAGE and DGKK proteins were up-regulated, which implied that AGER/RAGE and DGKK activation might be related to oxidative stress, leading to hepatoma cell death. Conclusions: Our studies identified multiple mechanisms are responsible for the anti-tumour activity of ascorbate and suggest high doses of ascorbate with less frequency will act as a novel therapeutic agent for liver cancer in vivo ., Competing Interests: Competing Interests: The authors have declared that no competing interest exists., (© The author(s).)- Published
- 2019
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19. Anastomosing haemangioma of liver: A case report.
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Peng X, Li J, and Liang Z
- Abstract
Anastomosing haemangioma (AH) is a recently described, unusual variant of capillary hemangioma that appears to be unique to the genitourinary system, with a particular proclivity for the kidney. AH is a subtype of capillary haemangioma, which is rarely encountered in clinical practice, particularly in the liver. We herein present the case of a 57-year-old woman with an incidental finding on magnetic resonance imaging of a local lesion in the liver, sized 3.3×3.0 cm. The patient underwent hepatectomy with a good postoperative recovery. The histopathological diagnosis was AH of the liver. To the best of our knowledge, this is the first case report of hepatic AH.
- Published
- 2017
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20. Effect of immobilization stress on the appetite and stomach ghrelin expression in maternal mice.
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Li B, Xu Y, Pan D, Xiao Q, Gao Q, Chen X, Peng X, Du Y, and Gao P
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- Agouti Signaling Protein metabolism, Animals, Corticotropin-Releasing Hormone metabolism, Disease Models, Animal, Eating, Female, Ghrelin genetics, Hypothalamus metabolism, Hypothalamus physiopathology, Lactation genetics, Leptin metabolism, Mice, Inbred ICR, Neuropeptide Y metabolism, Postpartum Period, Pro-Opiomelanocortin metabolism, Receptor, Serotonin, 5-HT2B metabolism, Receptor, Serotonin, 5-HT2C metabolism, Receptors, Ghrelin metabolism, Stress, Psychological genetics, Stress, Psychological physiopathology, Stress, Psychological psychology, Time Factors, Weight Gain, Appetite Regulation, Feeding Behavior, Gastric Mucosa metabolism, Ghrelin metabolism, Lactation metabolism, Maternal Exposure, Restraint, Physical psychology, Stress, Psychological metabolism
- Abstract
Maternal stress exerts long-lasting postnatal growth on offspring, which persist into adulthood. However, the effect of maternal stress on appetizing system has not been widely reported. In this study, we found that maternal immobilization stress (IS) during lactation resulted in low body weight and food intake. Immunohistochemistry showed an increase in stomach ghrelin protein expression. The central regulation of body weight and food intake occurs in the hypothalamus, which contains multiple neuronal systems that play important roles in the regulation of energy homeostasis. These systems including multiple neuropeptides involve in the ghrelin pathway of appetite regulation. Therefore, real time reverse transcription polymerase chain reaction (RT-PCR) was used to measure the change of mRNA expression of ghrelin pathway related hormones in order to explore the mechanisms involved in the appetite regulation. Expression levels of the hypothalamic 5-hydroxytryptamine 2c receptor (5-HT2cR) and 5-HT2bR, which are essential for the development and function of ghrelin and leptin, were decreased, as well as those of corticotrophin releasing factor (CRF) and pro-opiomelanocortin (POMC). While the expression of growth hormone secretagogue receptor (GHSR), neuropeptide-Y (NPY) and agouti-related protein (AgRP) showed an increase with significant difference. These results suggest that stress in a postpartum mother has persistent effects on the body weight of their offspring. Increased ghrelin and decreased leptin expression in the stomach may play a role in these effects.
- Published
- 2015
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