1. Antifungal activity and mechanism of Litsea cubeba (Lour.) Persoon essential oil against the waxberry spoilage fungi Penicillium oxalicum and its potential application.
- Author
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Yu, Liang, Zhou, Yangxia, Chen, Yicun, Wang, Yangdong, Gu, Qing, and Song, Dafeng
- Subjects
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ESSENTIAL oils , *PENICILLIUM , *SCANNING transmission electron microscopy , *GIBBERELLA fujikuroi , *PENICILLIUM chrysogenum , *GAS chromatography/Mass spectrometry (GC-MS) - Abstract
Litsea cubeba essential oil (LCEO) is a broad-spectrum bacteriostatic substance produced from the fruit of the Litsea tree that has been used for the treatment of various diseases in China for thousands of years. Here, the antifungal activities of LCEO against 10 different fungi (Naganishia diffluens, Fusarium sacchari, Cladosporium tenuissimum, Fusarium proliferatum, Fusarium verticillioides, Fusarium subglutinans, Mucor racemosus, Penicillium oxalicum, Penicillium chrysogenum , and Aspergillus niger) that cause rot to waxberries were assessed. The chemical components of LCEO and its modes of action against P. oxalicum were investigated. Citral (32.62 %) was characterized as the main component of LCEO by gas chromatography–mass spectrometry. LCEO exhibited excellent antifungal activities against all 10 fungi. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration of LCEO against P. oxalicum were 2.24 and 4.48 g/L, respectively. Furthermore, LCEO (MIC) compromised membrane permeability and integrity, caused leakage of the cell components, and increased production of malondialdehyde and reactive oxygen species. Scanning electron microscopy and transmission electron microscopy indicated that the morphology and ultrastructure of the LCEO-treated hyphal cell membrane and organelles were severely damaged. Meanwhile, LCEO increased the shelf life of waxberries from 1–2 to 5–6 d. LCEO is a potential ecologically friendly alternative to commercial fungicides to inhibit postharvest fungal contamination of waxberries during shipment and storage. [Display omitted] • Components of Litsea cubeba essential oil (LCEO) were identified by GC–MS. • LCEO showed outstanding inhibitory effects against ten fungal pathogen of waxberry. • LCEO damaged the plasma and mitcchondrial membrane of P. oxalicum. • LCEO resulted in the leakage of nucleic acids and soluble sugar from the mycelia. • LCEO is an alternative of synthetic preservatives to reduce decay in waxberry. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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