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This article, published in Advanced Science, discusses the role of EZH2 inhibitors in colorectal cancer. The study found that EZH2 inhibitors can activate the expression of deubiquitinase USP22, which stabilizes the PD-L1 protein and enhances its expression. This leads to immune evasion and compromised anti-tumor efficacy. The article suggests that combining EZH2 inhibitors with immunotherapy can overcome these immune suppressive effects and improve treatment outcomes. The full details of the study can be found in the article by Hao Wang, Jianling Yang, Lixiang Xue, and their colleagues. [Extracted from the article]

The basic leucine zipper (bZIP) transcription factors (TFs) function importantly in numerous life processes in plants. However, bZIP members and their biological roles remain unknown in Camelina sativa , a worldwide promising oil crop. Here, 220 CsbZIP proteins were identified in camelina and classified into thirteen groups. Two and 347 pairs of tandem and segmental duplication genes were detected to be underwent purification selection, with segmental duplication as the main driven-force of CsbZIP gene family expansion. Most CsbZIP genes displayed a tissue-specific expression pattern. Particularly, CsbZIP-A12 significantly positively correlated with many FA/oil biosynthesis-related genes, indicating CsbZIP-A12 may regulate lipid biosynthesis. Notably, yeast one-hybrid (Y1H), β-Glucuronidase (GUS), dual-luciferase (LUC) and EMSA assays evidenced that CsbZIP-A12 located in nucleus interacted with the promoters of CsSAD2–3 and CsFAD3–3 genes responsible for unsaturated fatty acid (UFA) synthesis, thus activating their transcriptions. Overexpression of CsbZIP-A12 led to an increase of total lipid by 3.275 % compared to the control, followed with oleic and α-linolenic acid levels enhanced by 3.4 % and 5.195 %, and up-regulated the expressions of CsSAD2–3 , CsFAD3–3 and CsPDAT2–3 in camelina seeds. Furthermore, heterogeneous expression of CsbZIP-A12 significantly up-regulated the expressions of NtSAD2 , NtFAD3 and NtPDAT genes in tobacco plants, thereby improving the levels of total lipids and UFAs in both leaves and seeds without negative effects on other agronomic traits. Together, our findings suggest that CsbZIP-A12 upregulates FA/oil biosynthesis by activating CsSAD2–3 and CsFAD3–3 as well as possible other related genes. These data lay a foundation for further functional analyses of CsbZIPs, providing new insights into the TF-based lipid metabolic engineering to increase vegetable oil yield and health-beneficial quality in oilseeds. [ABSTRACT FROM AUTHOR]

• A carbon-based electrocatalyst (BN-NLC) was developed. • Produced-syngas composition can be easily regulated on BN-NLC. • A wide tunable syngas of 0.2 ∼ 3.2 (H2 / CO) was achieved. Syngas generation by cost-effective carbons through CO 2 reduction reaction contributes greatly to environmental CO 2 mitigation. Herein, a series of B, N co-doping nanolayered carbon (BN-NLC) electrocatalysts are developed by molten-salt pyrolysis. The results show that, the products of CO 2 electroreduction on as-prepared BN-NLC are CO and H 2 with no liquid phase product. And the optimal BN-NLC (900 °C, n (C6H5)4BNa : n melamine =1:200) exhibits high selectivity (the faradaic efficiency of CO and H 2 is 83.45 % and 16.54 %, respectively) with the syngas ratio from 0.2 to 3.2 (H 2 / CO), easily regulated by controlling the applied potential during the CO 2 reduction process. This study would provide a good option for the electrochemical CO 2 reduction to produce tunable syngas feedstock for numerous downstream processes (e.g. Fischer-Tropsch process and hydroformylation of alkenes to aldehydes). [Display omitted] [ABSTRACT FROM AUTHOR]

Viscum coloratum (Kom.) Nakai has been traditionally used in China for nearly a thousand years to treat rheumatic diseases. However, its efficacy and mechanisms in treating rheumatoid arthritis (RA) have not been demonstrated. To investigate the anti-arthritic effects and molecular mechanisms of Viscum coloratum (Kom.) Nakai on collagen-induced arthritic mice through network pharmacology technology and experimental validation. First, the main ingredients of the extract of Viscum coloratum (Kom.) Nakai (EVC) were identified through chemical composition characterization using Ultra Performance Liquid Chromatography Tandem Mass Spectrometry (UPLC-MS). Then, the collagen-induced arthritis (CIA) model was established in DBA/1 J mice and the ameliorative effects of EVC on the progression of CIA mice were evaluated by oral treatment with different doses of the EVC for 28 days. After that, cytokine antibody microarray assay was used to detect the levels of multiple inflammation-related cytokines and chemokines in each group, and performed Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG) enrichment analysis. Subsequently, the potential target for the effective chemical components of EVC in treating RA was identified using various databases. Additionally, a drug-disease target protein-protein interaction network (PPI) was conducted using Cytoscape for visualization and clustering, while GO and KEGG enrichment analyses were performed with the Metascape database. Finally, identified phenotypes and targets by network pharmacology analysis were experimentally validated in vivo. Treatment with EVC significantly suppressed the severity of CIA with a dramatic reduction of paw swelling, arthritis index, levels of IgGs (IgG, IgG1, IgG2a, and IgG2b), multi-inflammation-related cytokines and chemokines on the progression of CIA. Histopathological examinations showed EVC could markedly inhibit inflammatory cell infiltration, tartrate-resistant acid phosphatase (TRAP) activity of osteoclast, and bone destruction. Furthermore, GO and KEGG enrichment analyses revealed that EVC could ameliorate RA by inhibiting osteoclast differentiation and regulating multiple signaling pathways including Osteoclast differentiation, IL-17, and TNF. PPI network analysis demonstrated that AKT1, MMP9, MAPK3, and other genes were highly related to EVC in treating RA. Finally, we proved that EVC could inhibit the expression of NFTAc1, MMP9, Cathepsin K, and AKT which were closely related to osteoclast activity. EVC could treat RA through multiple components, multiple targets, and multiple pathways. The present study demonstrated the therapeutic efficacy of EVC and its molecular mechanisms in treating RA, indicating that it would be a potent candidate as a novel botanical drug for further investigation. [Display omitted] • EVC has similar therapeutic effects as Chinese medicinal formulae in treating RA. • EVC can treat RA by inhibiting multiple inflammatory cytokines and chemokines. • EVC contains a rare class of diphenylheptanes. [ABSTRACT FROM AUTHOR]

The basic leucine zipper (bZIP) transcription factors (TFs) function importantly in numerous life processes in plants. However, bZIP members and their biological roles remain unknown in Camelina sativa , a worldwide promising oil crop. Here, 220 CsbZIP proteins were identified in camelina and classified into thirteen groups. Two and 347 pairs of tandem and segmental duplication genes were detected to be underwent purification selection, with segmental duplication as the main driven-force of CsbZIP gene family expansion. Most CsbZIP genes displayed a tissue-specific expression pattern. Particularly, CsbZIP-A12 significantly positively correlated with many FA/oil biosynthesis-related genes, indicating CsbZIP-A12 may regulate lipid biosynthesis. Notably, yeast one-hybrid (Y1H), β-Glucuronidase (GUS), dual-luciferase (LUC) and EMSA assays evidenced that CsbZIP-A12 located in nucleus interacted with the promoters of CsSAD2–3 and CsFAD3–3 genes responsible for unsaturated fatty acid (UFA) synthesis, thus activating their transcriptions. Overexpression of CsbZIP-A12 led to an increase of total lipid by 3.275 % compared to the control, followed with oleic and α-linolenic acid levels enhanced by 3.4 % and 5.195 %, and up-regulated the expressions of CsSAD2–3 , CsFAD3–3 and CsPDAT2–3 in camelina seeds. Furthermore, heterogeneous expression of CsbZIP-A12 significantly up-regulated the expressions of NtSAD2 , NtFAD3 and NtPDAT genes in tobacco plants, thereby improving the levels of total lipids and UFAs in both leaves and seeds without negative effects on other agronomic traits. Together, our findings suggest that CsbZIP-A12 upregulates FA/oil biosynthesis by activating CsSAD2–3 and CsFAD3–3 as well as possible other related genes. These data lay a foundation for further functional analyses of CsbZIPs, providing new insights into the TF-based lipid metabolic engineering to increase vegetable oil yield and health-beneficial quality in oilseeds. [ABSTRACT FROM AUTHOR]

The gut fungal community represents an essential element of human health, yet its functional and metabolic potential remains insufficiently elucidated, largely due to the limited availability of reference genomes. To address this gap, we presented the cultivated gut fungi (CGF) catalog, encompassing 760 fungal genomes derived from the feces of healthy individuals. This catalog comprises 206 species spanning 48 families, including 69 species previously unidentified. We explored the functional and metabolic attributes of the CGF species and utilized this catalog to construct a phylogenetic representation of the gut mycobiome by analyzing over 11,000 fecal metagenomes from Chinese and non-Chinese populations. Moreover, we identified significant common disease-related variations in gut mycobiome composition and corroborated the associations between fungal signatures and inflammatory bowel disease (IBD) through animal experimentation. These resources and findings substantially enrich our understanding of the biological diversity and disease relevance of the human gut mycobiome. [Display omitted] • A cultivated gut fungi (CGF) catalog (760 genomes, 206 species) was established • CGF catalog illustrates the functional and primary metabolic diversity of gut fungi • CGF catalog characterizes the gut mycobiome in Chinese and non-Chinese populations • Fungal signatures were identified in common diseases and validated in colitis mice This study offers a catalog of cultivated gut fungi (CGFs) comprising 760 genomes derived from healthy individuals. This compendium highlights the taxonomic and functional diversity and metabolic potential of human gut fungi, aids in constructing a comprehensive phylogenetic representation of the gut mycobiome, and sheds light on common disease-related variations. [ABSTRACT FROM AUTHOR]

The tripartite motif family, predominantly characterized by its E3 ubiquitin ligase activities, is involved in various cellular processes including signal transduction, apoptosis and autophagy, protein quality control, immune regulation, and carcinogenesis. Tripartite Motif Containing 15 (TRIM15) plays an important role in melanoma progression through extracellular signal-regulated kinase activation; however, data on its role in pancreatic tumors remain lacking. We previously demonstrated that TRIM15 targeted lipid synthesis and metabolism in pancreatic cancer; however, other specific regulatory mechanisms remain elusive. We used transcriptomics and proteomics, conducted a series of phenotypic experiments, and used a mouse orthotopic transplantation model to study the specific mechanism of TRIM15 in pancreatic cancer in vitro and in vivo. TRIM15 overexpression promoted the progression of pancreatic cancer by upregulating the toll-like receptor 4. The TRIM15 binding protein, IGF2BP2, could combine with TLR4 to inhibit its mRNA degradation. Furthermore, the ubiquitin level of IGF2BP2 was positively correlated with TRIM15. TRIM15 could ubiquitinate IGF2BP2 to enhance the function of phase separation and the maintenance of mRNA stability of TLR4. TRIM15 is a potential therapeutic target against pancreatic cancer. • TRIM15 enhances PDAC cells metastasis and progression by regulating the level of TLR4 in vitro and in vivo. • The K63 ubiquitination caused by TRIM15 can strength the phase separation of IGF2BP2 to activate IGF2BP2. • IGF2BP2 can recognize and bind TLR4 mRNA to improve the stability of TLR4. • TRIM15/IGF2BP2/TLR4 regulatory axis induces PDAC cells metastasis and progression via K63 ubiquitination, phase separation and RNA methylation. [ABSTRACT FROM AUTHOR]

Small extracellular vesicles (sEVs) residing at tumor tissues are valuable specimens for biopsy. Tumor heterogeneity is common across all cancer types, but the heterogeneity of tumor tissue-derived sEVs (Ti-sEVs) is undefined. This study aims to discover the spatial distributions of Ti-sEVs in oral squamous cell carcinoma (OSCC) tissues and explore how these vesicle distributions affect the patients' prognosis. Multi-regional sampling enabled us to uncover that Ti-sEVs' accumulation at peritumoral sites correlates with a higher disease-free survival rate, and conversely, sparse peritumoral Ti-sEVs tend to forecast a higher risk of relapse. Of those relapsed patients, Ti-sEVs strongly bind to extracellular matrix and subsequently degrade it for allowing themselves enter the bloodstream rather than staying in situ. In advanced OSCC patients, the quantity and spatial distribution of Ti-sEVs prior to anti-PD-1 treatment, as well as the temporal variance of Ti-sEVs before and after immunotherapy, strongly map the clinical response and can help to distinguish the patients with shrinking tumors from those with growing tumors. Our work elucidates the correlation of spatiotemporal features of Ti-sEVs with patients' therapeutic outcomes and exhibit the potential for using Ti-sEVs as a predictor to forecast prognosis and screen the responders to anti-PD-1 therapy. • Ti-sEVs exhibit strong spatiotemporal heterogeneity. • Spatial distribution of Ti-sEVs correlates with tumor relapse. • Spatial-temporal distribution of Ti-sEVs is associated with anti-PD-1 response. [ABSTRACT FROM AUTHOR]

• A broad-spectrum peptide was obtained for RBD and variants purification. • Multistep virtual screening was performed for the screening of candidate peptides. • LP14 peptide had submicromolar affinities to RBD and omicron, XBB, and BQ RBDs. • Adsorption and elution buffers of RBD on LP14-SepFF column were optimized. • RBD and omicron RBD could be purified by LP14-SepFF column from complex feedstock. Since the outbreak of coronavirus disease 2019, the global demand for vaccines has increased rapidly to prevent infection and protect high-risk populations. However, identifying viral mutations poses an additional challenge for chromatographic purification of vaccines and subunit vaccines. In this study, a new affinity peptide model, X 1 VX 2 GLNX 3 WX 4 RYSK, was established, and a library of 612 peptides was generated for ligand screening. Based on a multistep strategy of ligand screening, 18 candidate peptides were obtained. The top ranking peptide, LP14 (YVYGLNIWLRYSK), and two other representative peptides, LP02 and LP06, with lower rankings were compared via molecular dynamics simulation. The results revealed that peptide binding to the receptor binding domain (RBD) was driven by hydrophobic interactions and the key residues involved in the binding were identified. Surface plasmon resonance analysis further confirmed that LP14 had the highest affinity for the wild RBD (K d =0.520 μmol/L), and viral mutation had little influence on the affinity of LP14, demonstrating its great potential as a broad-spectrum ligand for RBD purification. Finally, chromatographic performance of LP14-coupled gel-packed column verified that both wild and omicron RBDs could be purified and were eluted by 0.1 mol/L Gly-HCl buffer (pH 3.0). This research identified a broad-spectrum peptide for RBD purification based on rational design and demonstrated its potential application in the purification of RBDs from complex feedstock. [ABSTRACT FROM AUTHOR]

This study introduces two novel sandwich-type tungsten–oxygen cluster compounds synthesized by hydrothermal methods, H4(C6H12N2H2)3{Na(H2O)2[Mn2(H2O)(GeW9O34)]}2 (Compound 1) and H2(C6H12N2H2)3.5{Na3(H2O)4[Co2(H2O)(GeW9O34)]2}·17H2O (Compound 2). The two compounds comprise cluster anions [GeW9O34]10– coordinated with transition metal atoms, either Mn or Co, and are stabilized by organic ligands. These compounds are crystallized in the hexagonal crystal system and P63/m space group. The two compounds were characterized through various techniques. Fourier transform infrared (IR) spectroscopy showed absorption peaks of anionic backbone vibrations of the Keggin cluster at 500–1000 cm–1, IR spectral peaks of δ(N–H) and νas(C–N) of the ligand triethylenediamine at 1000–2000 cm–1, and IR spectral peaks of the ligand νas(N–H) and νas(O–H) of water at 3000–3500 cm–1. Despite similar one-dimensional (1D) IR spectra due to the same cluster anions and similar molecular structures, the two compounds exhibited distinct responses in two-dimensional correlation spectroscopy with IR under magnetic and thermal perturbations. Under magnetic perturbation, Compound 1 showed a strong response peak for νas(W–Ob–W), while Compound 2 exhibited a strong response peak for νas(W=Od), possibly linked to differing magnetic particles. Similarly, Compound 1 displayed a strong response peak under thermal perturbation for νas(W–Oc–W). In contrast, Compound 2 showed a strong response peak for νas(W=Od); these results may be attributed to the different hydrogen bonding connections between the two compounds, which affect the groups in distinct ways through vibration and transmit these vibrations to the W–O bonds. The research presented in this paper expands the theoretical and experimental data of 2D correlation IR spectroscopy. [ABSTRACT FROM AUTHOR]

The number of terminal devices has skyrocketed along with the quick growth of cognitive radio networks. Massive equipment produce a lot of data that should not be shared, often WiFi signals. The radio frequency (RF) fingerprint identification approach for WiFi signals proposed in this research is based on federated learning and trains a collaborative model to complete RF fingerprint without transferring privacy-sensitive data. Aiming at the lack of labeled data and heterogeneous distribution of labeled data in actual situations, a federated transfer learning mechanism is designed. The technique suggested in this paper increases the accuracy of RF fingerprint at various sizes and assures that data privacy is not compromised, according to experimental results on real-world datasets. [ABSTRACT FROM AUTHOR]

[Display omitted] • A simple strategy for achieving efficient doping-free TADF OLEDs was developed. • Multiple shielding groups reduced ACQ effect and enhanced PLQY of neat film. • The ultrathin doping-free OLEDs achieved unprecedented EQE over 30 % at 620 nm. The application of doping-free emitting layers in organic light-emitting diodes (OLEDs) device fabrication has attracted extensive attention due to the simplified process and the low cost. Meanwhile, the application of interfacially doping layer (or ultrathin emissive layer, UEML) with a thickness below 1 nm showed great potential in achieving high device efficiency by reducing the aggregation-caused quenching (ACQ) effect, which was usually observed in neat film and highly doped dopant-host system. This work developed a simple molecular design strategy via peripheral decoration of TADF emissive core with multiple shielding groups (t -butyl and aryl units) to effectively alleviate the intermolecular interaction and the ACQ effect. The resulting OLEDs based on BIPH-TPA exhibited a record-setting external quantum efficiency (EQE) of 15.8 % at 640 nm with a conventional doping-free configuration (25 nm). Furthermore, the ultrathin doping-free (0.2 nm) OLEDs based on NA-TPA achieved unprecedented EQE of 33.1 % at 620 nm. The outstanding EL performance clearly demonstrated promising potential of this molecular design strategy for the exploration of doping-free OLEDs. [ABSTRACT FROM AUTHOR]

[Display omitted] • A series of V-based Si-group intermetallic compounds were successfully synthesized. • The excellent HER activity of V 3 Si in V-based Si-group intermetallic compounds. • High electronic conductivity and a suitable d -band center contributed to an excellent performance by HER. • Providing the fundamental guidance of rational design of desirable silicide electrocatalysts. Exploring high-efficiency and low-cost electrocatalysts is valuable for water-splitting technologies. Recently, Si-group compounds have attracted increasing attention in electrocatalysis, considering the abundant Si-group elements on Earth. However, Si-group compounds for HER electrocatalysis have not been systematically studied. In this study, we unveil the activity trends of non-noble metal catalyst A15-type V 3 M (i.e. , V 3 Si, V 3 Ge, and V 3 Sn) superconductors and show that V 3 Si is the most efficient HER catalyst because of the high electronic conductivity and suitable d -band center. Among them, the V 3 Si only requires 33.4 mV to reach 10 mA cm−2, and only 57.6 mV and 114.6 mV are required to attain a high current density of 100 mA cm−2 and 500 mA cm−2, respectively. These low overpotentials are close to the 34.3 mV at 10 mA cm−2 of state-of-art Pt/C (20 %) but superior to 168.5 mV of Pt/C (20 %) at 100 mA cm−2. Furthermore, the V 3 Si illustrates exceptional durability with no obvious decay in the 120 h at the different current densities (i.e. , 10–250 mA cm−2). The excellent HER activity of V 3 Si alloy can be ascribed to the synergies of superior electronic conductivity and suitable d -band center. Moreover, DFT calculations reveal that |ΔG H* | is decreased after introducing the V to Si. Beyond offering a stable and high-performance electrocatalyst in an acidic medium, this work inspires the rational design of desirable silicide electrocatalysts. [ABSTRACT FROM AUTHOR]

The rare-earth chalcogenide Er 2 Te 3 exhibits substantial promise as an innovative thermoelectric material. However, there have been limited studies exploring its thermoelectric properties in depth. Our study employed a first-principles approach in conjunction with the semi-classical Boltzmann transport theory to investigate the collaborative modulation of the thermoelectric transport properties of Er 2 Te 3 through the concurrent application of sulfur-group elements doping and strain engineering. The findings demonstrate that, in the presence of the synergistic interplay of doping and strain, the maximum power factor (PF) of p-type Er 2 Te 3 at 300 K increases to approximately 1.8 mW·m−1·K−2, while n-type Er 2 Te 3 is elevated to approximately 18 mW·m−1·K−2. Furthermore, the cooperative effects of doping and strain augmentation raise the optimal thermoelectric figure of merit (ZT) of p-type Er 2 Te 3 material to 0.3 at 300 K and n-type Er 2 Te 3 material to 1.35 when subjecting the n-type Er 2 Te 2.82 Po 0.18 system to a −2% strain. These results suggest that even minor doping can yield similar enhancements in the thermoelectric performance of Er 2 Te 3 , particularly under conditions of smaller strain. Consequently, our work underscores the significance of synergistic interactions between doping and strain engineering as potent means to augment the thermoelectric performance of Er 2 Te 3 materials. In light of the practical feasibility of the fabrication techniques employed, this novel rare-earth chalcogenide material warrants further scrutiny and subsequent exploration within experimental domains. [Display omitted] • The effectiveness of combining doping and strain to improve the thermoelectric performance of Er 2 Te 3. • This innovative rare-earth chalcogenide material Er 2 Te 3 is a promising thermoelectric material. • It as a promising contender for room-temperature thermoelectric applications. [ABSTRACT FROM AUTHOR]

Ru-modified monolayer-dispersed WO x /CeO 2 hybrid composites were prepared by co-impregnation (CI) and step impregnation (SI) methods, and the effects of active site distribution on the catalytic oxidation of 1,2-dichloroethane (DCE) were investigated. Ru species tends to be deposited on the monolayer-dispersed WO x (m-WO x) by SI method, which can increase the oxygen vacancies (O V,m-WOx) at m-WO x /CeO 2 interfaces. Abundant O V,m-WOx can promote the formation of more active W–OH and accelerate the dechloridation and oxydehydrogenation of DCE. Oppositely, for CI method, Ru species exists mainly in the form of Ru−O−Ce bonds, increasing the oxygen vacancies (O V,RuCe) into CeO 2 surface lattices and promoting the deep oxidation of intermediate products. The closer contact between the two hetero-interfacial oxygen vacancies (O V,RuCe and O V,m-WOx) on Ru-modified m-WO x /CeO 2 produces a stronger synergistic effect on DCE activation and oxidation, and meanwhile advantageously inhibits the adsorption of chlorine species as well as the formation of polychlorinated by-products. [Display omitted] • Impregnation sequence affects active site distribution on Ru-modified m-WO x /CeO 2 catalysts. • Ru species on m-WO x surfaces increases O V,m-WOx and W–OH by SI method. • CI method generates more Ru−O−Ce and O V,RuCe. • Synergies of hetero-interfacial O V promote DCE activation/oxidation and inhibit Cl species absorption. [ABSTRACT FROM AUTHOR]

Ce: YAG- Al 2 O 3 composite ceramics are regarded as highly promising materials for laser illumination application due to their high luminous efficacy and thermal stability. Nevertheless, the Ce: YAG ceramics exhibit limited color rendering index (CRI) for indoor lighting due to their low red emission component. In this study, we synthesized high-performance Ce, Mn-doped (Y, Tb) 3 Al 5 O 12 –Al 2 O 3 composite ceramic phosphors via a vacuum solid-state sintering method. It was observed that the introduction of Mn2+ ions created a second luminescence center with an emission peak centered at 580 nm. As the Mn2+ concentration increased, the emission peak redshifted from 580 nm to 591 nm, leading to a significant enhancement in the color rendering index of the ceramics. The cross-energy transfer between Tb3+ and Mn2+, Ce3+ ions augmented the absorption and conversion of blue light by the ceramics, thereby reducing the correlated color temperature greatly. Based on these findings, ceramics with a Ce3+ concentration of 3 at% and a Mn2+ concentration of 4 at% exhibited the highest CRI values (81.3 and 75.6) under both laser excitation and LED excitation, establishing them as strong candidates for achieving high color rendering in white LED/LD illumination applications. • The (Ce y Tb 0.1 Y 0.9-y) 3 (Al 1-2x Mn x Si x) 5 O 12 –Al 2 O 3 composite ceramic phosphors were fabricated successfully. • The energy transfer mechanism among Ce3+, Mn2+, and Tb3+ is discussed. • The introduction of Mn2+ ions created an emission peak centered at 580 nm, and the emission peak redshifted from 580 nm to 591 nm. • The ceramic exhibits the high CRI values (81.3 and 75.6) under both high-power laser excitation and LED excitation. [ABSTRACT FROM AUTHOR]

• This review focuses on affinity chromatography for virus-like particle purification. • Engineered affinity ligands and matrices for VLP purification were summarized. • Techniques for the rapid discovery of affinity peptides were described. • Design of universal ligands is crucial for addressing virus variation. • Robust platform for VLP purification is vital for addressing the epidemic outbreaks. The increasing medical application of virus-like particles (VLPs), notably vaccines and viral vectors, has increased the demand for commercial VLP production. However, VLP manufacturing has not yet reached the efficiency level achieved for recombinant protein therapeutics, especially in downstream processing. This review provides a comprehensive analysis of the challenges associated with affinity chromatography for VLP purification with respect to the diversity and complexity of VLPs and the associated upstream and downstream processes. The use of engineered affinity ligands and matrices for affinity chromatography is first discussed. Although several representative affinity ligands are currently available for VLP purification, most of them have difficulty in balancing ligand universality, ligand selectivity and mild operation conditions. Then, phage display technology and computer-assisted design are discussed as efficient methods for the rapid discovery of high-affinity peptide ligands. Finally, the VLP purification by affinity chromatography is analyzed. The process is significantly influenced by virus size and variation, ligand type and chromatographic mode. To address the updated regulatory requirements and epidemic outbreaks, technical innovations in affinity chromatography and process intensification and standardization in VLP purification should be promoted to achieve rapid process development and highly efficient VLP manufacturing, and emphasis is given to the discovery of universal ligands, applications of gigaporous matrices and platform technology. It is expected that the information in this review can provide a better understanding of the affinity chromatography methods available for VLP purification and offer useful guidance for the development of affinity chromatography for VLP manufacturing in the decades to come. [ABSTRACT FROM AUTHOR]

The scarcity of freshwater, the increasing use of brackish water (B) for irrigation, and the judicious application of organic fertilizers have significant research and practical value for environmental science, water resource management, and clean production. This study investigated the combined impact of B, magnetized-ionized brackish water (MIB), and organic fertilizer on pakchoi (Brassica chinensis L.) growth, yield, and soil quality. Over a two-year period (2022–2023), this study explored the impacts of two types of irrigation water (B and magnetized-ionized brackish water, MIB) in combination with varying rates of water-soluble bio-organic fertilizer (0, 20, 40, 60, and 80 kg/ha, corresponding to treatments 0 through 4, respectively). The findings indicated that the application of 60 kg/ha of organic fertilizer greatly improved the growth rate of pakchoi in terms of plant height, leaf area index, and fresh weight. Moreover, under MIB irrigation, the fresh weight growth rate of pakchoi was superior to that observed under B irrigation. Notably, B irrigation necessitated additional fertilizer to maintain elevated levels of alkali-hydrolyzed nitrogen (AN) and soil quality. Furthermore, the use of organic fertilizer substantially enhanced soil quality, with AN, available phosphorus (AP), organic matter (OM), and soil quality index (SQI) demonstrating a strong positive correlation (P < 0.05). Mantel test analysis showed significant correlations (P < 0.05) between soil OM, SWC, AP, and pakchoi growth and yield. According to the partial least squares path modeling analysis, the use of organic fertilizer had a relatively minor effect on the yield and quality of pakchoi under B irrigation, with path coefficients of 0.088 and 0.298, respectively. However, under MIB irrigation, organic fertilizer enhances yield and quality by improving soil quality (path coefficients of 0.914). In conclusion, the application of MIB irrigation coupled with organic fertilizer contributed to sustainable soil fertility, enhanced crop productivity, and mitigated the environmental impacts associated with conventional irrigation and fertilization methods. Our research underscores the importance of supporting agricultural research and development focused on sustainable irrigation and fertilization methods for farmers and policymakers. This integrated approach offers a harmonious solution for optimizing crop yields and enhancing water use efficiency, and the reduction of in chemical pollutants within agricultural production systems. • Organic fertilizer significantly increases pakchoi growth, especially under magnetized-ionized brackish water (MIB) irrigation. • Application of organic fertilizer leads to notable enhancements in soil nutrients and quality. • MIB irrigation requires less additional fertilizer compared to brackish water irrigation for optimal soil health. • The impact of organic fertilizer on crop yield and quality is more significant under MIB irrigation. [ABSTRACT FROM AUTHOR]

Imidazole propionate (ImP) is a detrimental metabolite produced by the fermentation of histidine intermediates via the intestinal flora. Here, the untargeted metabolite analysis of plasma metabolites from patients with diabetic nephropathy (DN), in combination with the Human Metabolome Database, revealed significantly increased levels of ImP in patients with DN, with a positive correlation with patients' blood creatinine concentration and urinary albumin-to-creatinine ratio, and a negative correlation with the glomerular filtration rate. RNA-seq was applied to detect the effects of ImP on renal tissue transcriptome in mice with DN. It demonstrated that ImP exacerbated renal injury in mice with DN and promoted renal tubular epithelial-mesenchymal transition (EMT), leading to renal mesenchymal fibrosis and renal impairment. Furthermore, ImP was found to directly target HAP90α and activate the PI3K-Akt signalling pathway, which is involved in EMT, by the drug affinity response target stability method. The findings showed that ImP may provide a novel target for DN quality, as it can directly bind to and activate HSP90, thereby facilitating the development of DN while acting as a potential indicator for the clinical diagnosis of DN. • Microbially produced ImP is elevated in the plasma of DN patients. • ImP promoted HK-2 cells EMT, leading to renal impairment in DN mice. • ImP directly targeted HAP90α and activated the PI3K-AKT signalling pathway. [ABSTRACT FROM AUTHOR]

This paper presents a high-resolution 18-bit SAR ADC with a high 10-bit capacitor DAC and a low 8-bit resistor DAC. The total required number of the unit capacitors is decreased to 512. Foreground digital calibration based on capacitive recombination is introduced to improve linearity. Preamplifiers and output offset storage(OOS) enhance the noise and offset performance of the comparator. As a result, the design under 180 nm process achieves a signal-to-noise and distortion ratio(SNDR) of 105.5dB and a spurious-free dynamic range (SFDR) of 116.3dB under 1 MS/s sampling rate with a single channel. The effective number of bits (ENOB) can reach 17.23 bits with a Nyquist-rate input while consuming 46 mW from a 5 V supply. The resultant Schreier and Walden figures of merit (FoM) are 178.92 dB and 295.34 fJ/conversion-step, respectively. The proposed SAR ADC occupies an actual area of 3850 μ\documentclass[12pt]{minimal}\usepackage{amsmath}\usepackage{wasysym}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{amsbsy}\usepackage{mathrsfs}\usepackage{upgreek}\setlength{\oddsidemargin}{-69pt}\begin{document}$$\upmu $$\end{document}m by 2810 μ\documentclass[12pt]{minimal}\usepackage{amsmath}\usepackage{wasysym}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{amsbsy}\usepackage{mathrsfs}\usepackage{upgreek}\setlength{\oddsidemargin}{-69pt}\begin{document}$$\upmu $$\end{document}m. [ABSTRACT FROM AUTHOR]

Patients with well-controlled low-density lipoprotein cholesterol (LDL-C) levels still suffer from the progress of the atherosclerotic cardiovascular disease (ASCVD) and can develop adverse outcomes. We conducted this study to analyze the relationship between elevated lipoprotein(a) [Lp(a)] levels and ASCVD risk. We enrolled 8070 patients in the ASCVD group and 440 participants in the non-ASCVD group [median age of 60 years; 6376 (74.9%) were male]. Multivariate logistic regression models were used to identify the relationships between the lipids and ASCVD. These models showed that the Lp(a) level was a significant independent risk factor for ASCVD [odds ratio (OR) = 1.025, confidence interval (CI) = 1.020–1.029, P < .001]. The different categories analysis showed the OR of the high Lp(a)/low LDL-C group was 9.612 [CI = 6.206–14.887], P < .001. Our study demonstrated that elevated Lp(a) levels were associated with the increased ASCVD risk. Also, the patients with low LDL-C but high Lp(a) levels still had a higher risk of developing ASCVD than the low Lp(a)/high LDL-C group. In addition, elevated Lp(a) levels were associated with a higher ASCVD risk in males, hypertensive, and diabetic patients. [ABSTRACT FROM AUTHOR]

[Display omitted] Graphical abstract showing the molecular mechanism of the changes in neurogenesis after SAH. OXCT1 regulated hippocampal ketone body metabolism and reduced neurogenesis through mechanisms mediated at least partially by the Akt/GSK-3β/β-catenin pathway after SAH. • Ketone oxidation and AHN decrease sharply in the early stage of SAH. • OXCT1 overexpression promotes AHN via activation of Akt/GSK-3β/β-catenin signaling. • LY294002 reverses the stimulative effect of ketone oxidation on neurogenesis. Subarachnoid hemorrhage (SAH) is a life-threatening neurological disease that usually has a poor prognosis. Neurogenesis is a potential therapeutic target for brain injury. Ketone metabolism also plays neuroprotective roles in many neurological disorders. OXCT1 (3-Oxoacid CoA-Transferase 1) is the rate-limiting enzyme of ketone body oxidation. In this study, we explored whether increasing ketone oxidation by upregulating OXCT1 in neurons could promote neurogenesis after SAH, and evaluated the potential mechanism involved in this process. The β-hydroxybutyrate content was measured using an enzymatic colorimetric assay. Adeno-associated virus targeting neurons was injected to overexpress OXCT1, and the expression and localization of proteins were evaluated by western blotting and immunofluorescence staining. Adult hippocampal neurogenesis was evaluated by dual staining with doublecortin and 5-Ethynyl-2′-Deoxyuridine. LY294002 was intracerebroventricularly administered to inhibit Akt activity. The Morris water maze and Y-maze tests were employed to assess cognitive function after SAH. The results showed that OXCT1 expression and hippocampal neurogenesis significantly decreased in the early stage of SAH. Overexpression of OXCT1 successfully increased hippocampal neurogenesis via activation of Akt/GSK-3β/β-catenin signaling and improved cognitive function, both of which were reversed by administration of LY294002. OXCT1 regulated hippocampal ketone body metabolism and increased neurogenesis through mechanisms mediated by the Akt/GSK-3β/β-catenin pathway, improving cognitive impairment after SAH. [ABSTRACT FROM AUTHOR]

• An effective deacidification of KHCO 3 addition in wine was investigated. • pH adjustment helped to initiate malo-lactic acid conversion in V. amurensis wines. • The addition of KHCO 3 after alcoholic fermentation enhanced wine aroma. • The pH of alcoholic fermentation dominated the level of esters and higher alcohols. • Sufficient malolactic fermentation helps to enhanced the global aroma. To produce quality dry red wines with high-acidity grapes of Vitis amurensis , an experiment was designed to adjust pH during winemaking by adding KHCO 3 at two time points and two pH levels in conjunction with malolactic fermentation (MLF). The organic acids and volatiles were detected by HPLC and GC–MS separately, combing with the color characteristic and sensory evaluation, we investigated the quality of V.amurensis wines under pH adjustment. Results showed that the pH adjustment weakened the wine color slightly but helped to initiate MLF. The low pH value of alcoholic fermentation favored the development of esters and higher alcohols. Higher pH levels promoted a sufficient MLF and enhanced the global aroma levels by 1.14–1.25 times, which led to higher sensory scores. In conclusion, KHCO 3 addition and MLF improved the quality of V. amurensis dry red wines, chemical addition after alcoholic fermentation was more effective for cold regions. [ABSTRACT FROM AUTHOR]

Stomatal conductance (g s) is an indicator that allows for direct evaluation of plant water status, but it is challenging to achieve rapid monitoring in large-scale fields due to limitations in observation methods. Here this study was conducted to identify the thresholds of g s with different target yields and develop a g s -based water stress diagnostic model for buffalograss (Buchloe dactyloides (Nutt.) Engelm.) using UAV thermal infrared imagery for buffalograss in 2022 and 2023. The results of the field experiment demonstrated that the g s rapidly response to changes in the water stress status of buffalograss. The thresholds of g s were 403 and 385 mmol m−2 s−1 for the vegetative and reproductive growth stages, respectively, with the target seed yield of 1224 kg ha−1. The g s values were classified into three levels for the vegetative growth and four levels for the in reproductive growth stage of buffalograss, respectively. The canopy temperature depression response to water stress is consistent with the g s. Based on this relationship, this study developed a g s -based diagnostic model with a random forest algorithm for buffalograss. Furthermore, a spital map of g s was created using UAV thermal infrared imagery. The modification test results indicated that the model made a good estimation of g s were good with normalized root mean square errors of 15% in the vegetative stage and 11% in the reproductive stage, respectively. Therefore, it is feasible to use thermal infrared imagery for monitoring g s and evaluating the water stress of plants in buffalograss fields. • Estimation stomatal conductance (gs) by UAV thermal infrared imagery was feasible. • Thresholds of g s were 403 and 385 mmol m−2 s−1 for two growth stages. • Diagnosis model with piecewise function performed well with NRMSEs of 11% to 15%. [ABSTRACT FROM AUTHOR]

Herein, the quenching method was employed to prepare ultra-low loading high-entropy catalyst for highly active, chemoselective, and robust furfural hydrogenation. The metals (Pd, Pt, Ru, Mo, Zn) in the form of a complex were vacancy anchored and strongly interacted with the support (TiO 2) during the quenching and reduction process, ultimately forming the high entropy catalyst PdPtRuMoZn-TiO 2 with strong metal and support interaction (SMSI) driven encapsulated layer. Moreover, this catalyst exhibits excellent catalytic performance for furfural selective hydrogenation with 90.82% conversion and 91.3% selectivity toward furfuryl alcohol at ambient temperature. The catalytic mechanism showed that high-entropy alloy (HEA) enhanced the hydrogen dissociation and migration capacity, and adjusted the electron environment of the interface between TiO 2 and HEA, causing rich oxygen vacancies in the TiO 2 SMSI driven encapsulated layer and the reduction of the horizontal furfural adsorption configuration, resulting in high FF conversion and furfuryl alcohol selectivity. The work reported ultra-low loading high-entropy catalyst and their superior performance in bio-cellulose-derived product conversion. [Display omitted] • Hyperdispersed HEA/TiO 2 catalysts were prepared via quenching and vacancy anchoring. • Content of HEA in the catalyst is only 0.33%. • HEA significantly enhanced the hydrogen spillover of catalysts. • Conversion of FFA and selectivity of FF is 90.82% and 91.3% at room temperature. • Electron transfer among HEA metal enhanced the catalytic activity. [ABSTRACT FROM AUTHOR]

A carbon tax is effective at curbing carbon emissions, but it is met with low public support due to its high personal cost. Investigations have been conducted to reform carbon tax design to ease the burden on individuals by providing economic compensation, but the cost for governments is high. We propose a new cost-efficient solution by introducing people to a pre-existing reward-based climate policy to create a sense of economic compensation. Across three experiments, we show that the presence of a pre-existing reward-based climate policy increases participants' support for a carbon tax, especially when the innate connection between the two policies is made salient and people regard the reward as compensation for the tax. In contrast, if people are distracted from sensing this interrelationship, support for the tax does not differ from when it is introduced alone. Applicability of this approach was tested under different conditions where the pressure to reduce carbon emission is either high or low. • Introducing a reward-based climate policy increases public support for the carbon tax. • Perceived economic compensation mediates the effect. • A national large-scale survey experiment and two online experiments were conducted. • This approach has the potential to be cost-effective. [ABSTRACT FROM AUTHOR]

• Licensed plasma derived and recombinant coagulation factors products were summarized. • Preparative separation procedures of recombinant coagulation factors were discussed. • Development of purification procedure of the recombinant products was highlighted. • Future challenges and considerations for new development are discussed. Hemostasis is a complex process for the cessation of bleeding from an injured blood vessel, involving the interplay of 12 coagulation factors in the coagulation cascade with activated blood platelets and the vessel wall. Hence, the coagulation factors are important to control hemorrhage. However, the low abundance of many coagulation factors in human plasma proteins limited their production in therapeutic drugs and their clinical applications. With the development of modern biotechnology, commercially manufactured recombinant coagulation factors became available as hemostatic therapeutics, emerging a huge potential in pharmaceutical manufacturing market. Unlike antibodies, whose standard operation unit or platform purification processes in the industrial-scale downstream processing has been well-established, the complexity in post-translational modification and differences in structures of the coagulation factors posed specific challenges with respect to the downstream processing, which have long been limiting their industrial-scale production. This review presents a comprehensive overview of the technological development of commercially manufactured recombinant coagulation factors, with emphasis on their advances and challenges in the separation and purification processes. Firstly, the licensed products of the plasma derived and recombinant coagulation factors are summarized. Then, typical recombinant coagulation factors, i.e. factors VII, VIII and IX, are introduced with detailed discussion on their preparative separation procedures for both the licensed products of industrial-scale and the experimental cases of laboratory-scale. Finally, perspectives and challenges in the future development of the purification technology of recombinant coagulation factors are highlighted to provide new insight into the design of cost-effective purification processes of recombinant coagulation factors. [ABSTRACT FROM AUTHOR]

• A paper-based assay was developed for real-time, onsite, and dual-signal detection of ATP. • The assay integrated the ATP-responsive ZIF-90 collapse and inhibition effect of ZIF-90 for nanozyme. • The analytical signals combined the distance- and colorimetric- readout. • A paper-based kit was designed to achieve standardized ATP onsite detection. Adenosine triphosphate (ATP) is an important biological biomarker, but its rapid extracellular decomposition poses challenges for on-site detection. We report a paper-based dual-readout viscosity flow sensor enabling rapid, equipment-free quantification of ATP. The assay integrates molybdenum disulfide nanozymes for signal amplification with zeolitic imidazolate framework-90 (ZIF-90) for selective ATP recognition and nanozyme regulation. In the presence of ATP, the intact chitosanase-encapsulated ZIF-90 structure was disintegrated to release chitosanase and thus hydrolyzed chitosan hydrogel, decreasing the viscosity of chitosan solution. Moreover, elimination of ZIF-90 inhibitory effect reactivated the catalytic activity of MoS 2 nanozyme, resulting in the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce obvious color change. The analytical signals, combining the distance- and colorimetric- readout, were directly displayed on filter paper without further processed. The proposed dual-readout viscosity flow sensor exhibited remarkable sensitivity for ATP detection with detection limits down to 0.038 nM with the distance-readout and 0.057 nM for the colorimetric-readout. Furthermore, we developed an integrated paper kit with a printed scale and color card for standardized on-site analysis without instrumentation. Successful application for ATP quantification in bacterial biofilms and blood samples demonstrates the sensor's strong performance and potential for decentralized testing. [ABSTRACT FROM AUTHOR]

Mesangial cells (MCs) in the kidney play central role in maintaining glomerular integrity, and their abnormal proliferation leads to major glomerular diseases including diabetic kidney disease (DKD). Although high blood glucose elicits MCs impairment, the underlying molecular mechanism is poorly understood. The present study aimed to investigate the effect of secreted frizzled-related protein 2 (Sfrp2) from single-nucleus RNA profiling on MC proliferation of DKD in vitro and in vivo and explored the specific mechanisms. By snRNA-seq analysis of isolated renal cells from leptin receptor-deficient db/db mice and control db/m mice, we found that Sfrp2 was increased in the MCs of DKD in comparison to other intrinsic renal cells, which was further verified in vitro and in vivo. We also found that the expression of Sfrp2 was significantly upregulated in DKD patients and correlated with renal function, demonstrating that Sfrp2 might serve as an independent biomarker for DKD patients. Functionally, we showed the loss and acquisition of Sfrp2 affected cytosolic Ca2+ concentration, cell proliferation and fibrosis of MC, albuminuria and kidney injury in vitro and in vivo. Mechanistically, we identify c-Jun as a transcription factor of Sfrp2 promoting its transcription, and the Ca2+ signaling related protein frizzled receptor 5 (Fzd5) as the binding protein of Sfrp2. And we further found Sfrp2 promoted Fzd5-induced cytosolic Ca2+ concentration and the downstream CaMKII/Mek/Erk pathway activation, leading to MC proliferation and fibrosis in DKD. Our study revealed a novel involvement for Sfrp2 in the regulation of MC function and the effect of Sfrp2 on cell proliferation and fibrosis of MC via the Fzd5/Ca2+/CaMKII/Mek/Erk pathway, implying that Sfrp2 may be a possible biomarker and therapeutic target for DKD. • Sfrp2, derived from snRNA-seq, is upregulated in vitro and in vivo and correlates with indicators of kidney injury. • Sfrp2 promotes Mesangial cells proliferation,further contributing to renal injury. • Sfrp2/Fzd5 activates CaMKII/Mek/Erk signal pathway to promote Mesangial cells proliferation. [ABSTRACT FROM AUTHOR]

[Display omitted] • Absolute neutrophil count in polycythemia vera patients was an independent risk factor indicative of thrombotic events. • The prothrombotic feature of neutrophils was associated with the activation of neutrophil extracellular traps pathway in polycythemia vera. • PRKCD-mediated neutrophil extracellular traps pathway might be activated in polycythemia vera patients. Thrombosis is a major cause of morbimortality in patients with polycythemia vera (PV). Furthermore, neutrophils play a significant role in thrombosis, but their role in the pathogenetic mechanisms of PV is not well characterized. Therefore, we investigated the role and mechanisms by which neutrophils regulate thrombosis in PV patients. U nivariate and multivariate logistic regression analysis of clinicopathological factors was performed to determine the independent risk factors of thrombosis in PV. Pearson's correlation analysis was performed to determine the relationship between absolute neutrophil count (ANC) and the hypercoagulable state in PV patients. Bioinformatics analysis of the GSE54644 dataset was used to identify hemostasis-related pathways in neutrophils of PV patients. Weighted gene co-expression network analysis (WGCNA) of the integrated dataset (GSE57793, GSE26049 and GSE61629) was used to identify neutrophils-related genes and pathways associated with thrombosis in PV. Ingenuity pathway analysis (IPA) was performed to identify the differentially activated pathways in PV patients with or without thrombosis using GSE47018 dataset. Our data showed increased ANC in PV patients. Multivariate logistic regression analysis showed that ANC was an independent risk factor for the thrombotic events in PV patients before or at diagnosis. ANC correlated with the hypercoagulable state in PV patients. Neutrophil extracellular traps (NETs) pathway was significantly enriched in the neutrophils of PV patients. IPA results demonstrated that PRKCD-mediated NETs pathway was hyperactivated in PV patients with thrombosis. In summary, ANC was an independent risk factor for the thrombotic events in PV patients before or at diagnosis, and PRKCD -mediated NETs pathway was aberrantly activated in the neutrophils of PV patients and was associated with the thrombotic events. [ABSTRACT FROM AUTHOR]

A novel multi-functional fluorescence probe HMIC based on hydrazide Schiff base has been successfully synthesized and characterized. It can distinguish Al3+/Zn2+/Cd2+ in ethanol, in which fluorescence emission with different colors (blue for Al3+, orange for Zn2+, and green for Cd2+) were presented. The limits of detection of HMIC towards three ions were calculated from the titration curve as 7.70 × 10− 9 M, 4.64 × 10− 9 M, and 1.35 × 10− 8 M, respectively. The structures of HMIC and its complexes were investigated using UV-Vis spectra, Job’s plot, infrared spectra, mass spectrometry, 1H-NMR and DFT calculations. Practical application studies have also demonstrated that HMIC can be applied to real samples with a low impact of potential interferents. Cytotoxicity and cellular imaging assays have shown that HMIC has good cellular permeability and potential antitumor effects. Interestingly, HMIC can image Al3+, Zn2+ and Cd2+ in the cells with different fluorescence signals. [ABSTRACT FROM AUTHOR]

Mahuang-Lianqiao-Chixiaodou decoction (MLCD) is a traditional Chinese medicinal (TCM) formula recorded in the Treatise on Febrile Diseases. It is commonly used for clinical treatment of atopic dermatitis (AD). However, the potential mechanisms of MLCD intervention in AD combined with mental disorders behaviors such as anxiety and depression remain elusive and deserves further investigation. The study aims to observe the effect of MLCD on anxiety- and depression-like behaviors in AD mice and explore the possible neuroinflammatory mechanism of NOD-like receptor 3 (NLRP3) inflammasome. The chemical components of MLCD extracts were identified using UHPLC-MS. The AD mice were induced by 2,4-dinitrofluorobenzene and treated with MLCD or mometasone furoate (MF, as a positive control) for 7 days. The pathological changes in their skin tissue and brain hippocampus were observed by hematoxylin-eosin staining. Elevated plus-maze test (EPM), open field test (OFT), and the suspended tail (TST) were used to measure the anxiety- and depressive-like behaviors in AD mice. Expression of NLRP3 inflammasome-related proteins in brain hippocampus were measured by the quantitative real-time polymerase chain reaction (qPCR) and western blotting (WB). We found that MLCD contain many active ingredients, including ephedrine, Forsythoside A, phillyrin, glycyrrhizic acid, etc. Both MLCD and MF alleviated skin lesions and promoted positive histopathological changes in the hippocampus of AD mince to varying degrees. MLCD however, could further increase their proportion of open arm entry times (Oentries%) in EPM, residence time in the central area (Ctime) and the proportion of the number of times in the central area (Centries%) in OFT significantly. MLCD also reduces their immobility time in TST considerably. Mechanistically, MLCD downregulated the relative mRNA expression and protein level of NLRP3, Caspase-1, IL-1β, and IL-18 in hippocampal tissue compared to the model group. MLCD can alleviate anxiety-like and depression-like behaviors in AD mice by intervening in the gene and protein expression of NLRP3 inflammasome-related factors, thus treating AD. [Display omitted] • Atopic dermatitis is characterized by recurrent lesions and intense pruritus as the main clinical manifestation. • Atopic dermatitis model is accompanied by anxiety-like depression-like mental disorder behavioral manifestations in mice. • Behavioural manifestations of mental disorders are associated with neuroinflammation in the hippocampus region of the brain. • Mahuang-Lianqiao-Chixiaodou decoction interventions alleviate AD symptoms and improve mood disorders. [ABSTRACT FROM AUTHOR]

Shuyu decoction (SYD), an effective traditional Chinese medicine (TCM), has been widely used for treating deficiency-related diseases for thousands of years. Meanwhile, exercise-induced fatigue (EF), a common physiological phenomenon observed in physical training, has been treated as a deficient condition in TCM for decades. Currently, not many studies have been conducted on the effect of SYD on EF and little is known about its underlying pharmacological mechanism. This current study was designed to assess the anti-fatigue roles of SYD and explore its effect on exercise-induced immune dysfunction. Eighteen rats were randomly divided into three groups: normal control (NC) group, model (M) group, and SYD group (27.8 g/kg). The M and SYD group were given treadmill training for 6 weeks. From the fourth week, the SYD group was administered SYD intragastrically for 3 consecutive weeks. After three weeks of treatment, the rats were anesthetized, and the blood and spleen tissue samples were dissected. The blood sample was devoted to the blood biochemical-related indicators, which were used to evaluate the anti-fatigue of SYD. The expression of Interleukin (IL)-6, IL-1β, tumor necrosis factor-α (TNF-α), IL-17, CD3+, and CD4+ were detected by ELISA and the level of CD8+ of blood was measured through Flow Cytometry (FC). The histopathological changes of spleen tissue samples were determined by Hematoxylin and eosin (H&E) staining and an estimation of CD3+, CD4+, and CD8+ expression of spleen tissues were calculated through FC. Compared with the M group, the SYD group observed an increase in tensile force and the ratio of cortisol to testosterone (TTE/COR), whereas a reduction in the levels of lactic acid (LAC), blood urea nitrogen (BUN), creatine kinase (CK), (P < 0.01 or P < 0.05). ELISA experiments showed that SYD reduced the expressions of IL-6, IL-1β, and TNF-α, IL-17 and increased the expression of IL-10 (P < 0.01 or P < 0.05). In the HE test, SYD treatment transformed the structure of the spleen. FC experiments further showed that SYD increased the expressions of CD3+, CD4+, and CD8+ in blood and spleen tissues (P < 0.01 or P < 0.05). Our findings indicate that SYD can alleviate EF by improving inflammation and immunity. However, the relationship between inflammatory factors and the related immune response remains to be further investigated. [Display omitted] • It is confirmed that exercise-induced fatigue can lead to immune dysfunction. • SYD can remarkablely alleviate exercise-induced fatigue by improving immune suppression and enhancing cellular immunity. • Its mechanism may be related to ameliorating muscle fatigue and inhibiting the secretion of inflammatory factors. [ABSTRACT FROM AUTHOR]

All-solid-state lithium battery (ALLSB) with organic/inorganic composite solid-state electrolyte (CSSE) is one of the candidates for future energy storage due to the high specific energy and safety. However, large impedances and incompatibilities at the rigid electrode/electrolyte and internal interfaces of CSSE remain stumbling blocks for the spread of ASSLB. Acrylamide (AM)-contained (3-methacrylamidopropyl) triethoxysilane (MTS) is incorporated into CSSE to optimize the interfaces and modulate the ion diffusion and deposition behaviors based on the in-situ synergy effects of coupling/crosslinking and hydrogen bonding. Multiple hydrogen bonds between CSSEs and polar AM groups construct diverse Li interactions and facilitate dissociation and diffusion of Li. Coupling/crosslinking effect of the MTS can in-situ rearrange the above diversified Li environment. In turn, hydrogen bonds strengthen the coupling effect to the interface. The synergistic function contributes to favorable interfacial compatibility and stability, establishing a fast ion transport channel in the ASSLB. The MTS-intervened CSSE exhibits superior electrochemical and thermal properties. Li symmetric cells with this functional CSSE demonstrate stable lithium deposition/extraction for up to 2000 h. Moreover, the Li|LiFePO 4 battery retains 91.7 % of its capacity after 500 cycles. This in-situ synergistic regulation provides a rational and simple guide for enhancing interface stability in CSSE and ASSLB. [Display omitted] • MTS was introduced into CSSEs to modulate interfaces in solid-state batteries. • Hydrogen bonds between MTS and CSSEs construct a diverse Li environment. • Coupling effects of the MTS can in-situ rearrange the above Li environment. • Hydrogen bonding and coupling/crosslinking effects co-tailor the interface. • Batteries with MTS-CSSE present fast and uniform Li+ diffusion/deposition. [ABSTRACT FROM AUTHOR]

The unregulated dendrite growth and deleterious derivative reactions at Zn anodes lie in the path of research and industrialization of aqueous Zn-ion batteries (AZBs). The pore of the separator is a natural sieve for ion diffusion, but the high energy barrier for transmembrane transport can cause the "bridging" effect of ion congestion at the pore entrance, and induce the incubation of dendrites instead. In this work, a continuous, stable and fast ion transport channel is constructed by in-situ guided cross-linking of zinc alginate (ZA) hydrogels through the porous membrane to conquer the negative pore effect. The homogeneity and continuity of the channel structure, as well as the high ionic conductivity and zincophilicity of the ZA, can homogenize the electric field and reduce the energy barrier for ion transport. In battery systems, the physical ion shunting effect of a homogeneous pore structure, combined with the chemical/electrochemical effects of ZA guiding the diffusion of Zn2+ and binding free water, combat zinc dendrites and interfacial side reactions. The novel electrolyte membrane enables a highly reversible Zn plating/stripping to stabilize the Zn anode. This work provides illuminating insights into the regulation and application of pore effects in porous electrolyte membranes in metal-based batteries. [Display omitted] • A fast ion channel is constructed by in-situ guided crosslinking of ZA hydrogels through the porous membrane. • The negative "bridging" pore effect is alleviated in this hydrogel membrane. • The ion shunting pore effect and the ion guiding function of ZA synergistically modulate the ion diffusion dynamics. • The Zn dendrites are suppressed and the electrochemical properties of the aqueous Zn-ion batteries are improved. [ABSTRACT FROM AUTHOR]

[Display omitted] • p -Di-ethyl-amino-methyl-thiacalix[4]arene (L) for Pt(IV) extraction was synthesized. • Extraction performance of L toward Pt(IV) was systematically investigated. • Complexation mechanism was clarified by slope analysis, SEM-EDS and 1H NMR titrations. • The complexation process was theoretically analyzed through DFT calculations. • Excellent extraction efficiency of Pt(IV) by using L as extractant was achieved. In the present study, the extraction performance and complexation mechanism of p -di-ethyl-amino-methyl-thiacalix[4]arene (L) toward Pt(IV) in a hydrochloric acid system were investigated. The effects of various parameters including solution pH, L to Pt(IV) mole ratio, Cl− concentration, acidity and reaction time on the complexation process of L with Pt(IV) were systematically investigated. The experimental results indicated that L has excellent complexation ability toward Pt(IV) that about 90 % of Pt(IV) could be extracted within 2 min under the optimal conditions. Moreover, L could selectively extract Pt(IV) from the HCl system containing multiple competing ions such as Ce(III), Zr(IV), La(III), Fe(III), Ni(II), Mg(II), and Al(III). The complexation mechanism between L and Pt(IV) was further clarified by slope analysis, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM-EDS), and proton nuclear magnetic resonance (1H NMR) titrations. Theoretical analysis of thermodynamics, complexation structure and intermolecular interactions between L and Pt(IV) during the solvent extraction process were performed through density functional theory (DFT) calculations. The results showed that the protonation of L occurs first to form the complex of L ·2HCl, which further reacts with PtCl 6 2− forming the complex of L ·2H+·PtCl 6 2− through anion-exchange, achieving the efficient and selective extraction of platinum. [ABSTRACT FROM AUTHOR]

Wafer fabrication is a highly complex manufacturing system. Using complex network models to portray the correlation between parameters is an effective tool for finding the key influencing factors associated with the cause of wafer defects. However, the complex network in a wafer fabrication system has many nonlinearities, non-normal distributions, coupling, and spurious correlations. Quantitative clarification of direct and indirect correlations in the complex network is the prerequisite for achieving explainable fault detection in smart manufacturing. Therefore, a Copula network deconvolution-based direct correlation disentangling framework is proposed in the context of explainable fault detection in semiconductor wafer fabrication. Firstly, The complex network correlation diagram of the parameters in the wafer fabrication process is constructed with each parameter as a node and the correlation coefficients between parameters as connected edges. Then, a nonlinear correlation metric model based on adaptive Copula function selection is designed to deal with the nonlinear correlation of complex network models. The adaptive selection of the Copula function is realized by the goodness-of-fit test method based on Euclidean distance. After that, considering the coupling and spurious correlation in the complex network model, a network deconvolution-based fault detection method is designed to identify direct correlation. Finally, a case study using the actual data from semiconductor wafer fabrication systems is conducted to compare the existing mainstream fault detection methods and validate the effectiveness of the proposed method. [ABSTRACT FROM AUTHOR]

• Latent class analysis identified seven patterns for school bullying perpetration and victimization. • There was an overlap between cyberbullying and traditional bullying. • Multiple social domain factors were associated with different patterns of school bullying. Adolescents involved in school bullying are a heterogeneous group. Yet less is known about school bullying patterns and their associated factors in social domains in China. The objective of the study was to use latent class analysis to identify patterns of school bullying involvement and to examine factors in individual, family, and school domains associated with each bullying pattern. Data were self-reported anonymously using a questionnaire completed by students from 3 middle schools and 4 high schools in a southern city in China (N = 13,166). A latent class analysis was conducted to identify involvement patterns in school bullying victimization and perpetration. Multinomial logistic regressions were used to examine factors in social domains associated with each pattern. Seven patterns of school bullying involvement were identified: Low Victim (14.5%), High Victim (4.4%), Sexual and Cyber Victim (2.5%), Physical Bully-Victim (1.4%), Verbal Bully-Victim (3.5%), All-Type Bully-Victim (3.3%) and Uninvolved (70.4%). Alcohol use, high frequency of using social networking sites, and distrusting people were associated with all bullying patterns (p <.05). Gender, grade level of schooling, boarding at school, cigarette smoking, being a class leader, poor academic performance, parents both working in other cities, poor relationships between family members, father's/mother's alcohol use, parental neglect, unpopularity at school, lack of feeling safe, and no close friends were each associated with at least one school bullying pattern (p <.05). Different types of school bullying victimization and perpetration co-occur among students and multiple social domain factors were associated with bullying patterns. Findings from this study suggest that consideration of the heterogeneity of school bullying and its associated factors are critical to developing targeted prevention interventions for different school bullying patterns. [ABSTRACT FROM AUTHOR]

The objective of this study was to investigate the effect of Lactobacillus salivarius ( L. salivarius ) SNK-6 supple-mentation on the laying performance, egg quality, blood parameters, intestinal morphology, and cecal microbial community of laying hens. A total of 432 healthy 30-wk-age laying hens were randomly divided into 3 groups with 6 replicates under the same husbandry and dietary regimes: control (CON); 2.0 × 108 CFU/kg L. salivarius supplementation (T1); 2.0 × 109 CFU/kg L. salivarius supplementation (T2). The experiment lasted for 10 wk. The results indicated that the supplementation resulted in a significant reduction in the broken egg and unqualified egg ratios, and a significant increase in the eggshell strength, eggshell relative weight, albumen height, and Haugh units (P < 0.05). The L. salivarius -treated hens exhibited significantly reduced serum malondialdehyde levels (P < 0.05); significantly increased total protein, phosphorus, calcitonin, and immunoglobulin M (P < 0.05); significantly increased cecal secretory immunoglobulin A concentration (P < 0.05); significantly improved villus height (VH) in the duodenum and VH to crypt depth ratio in the jejunum (P < 0.05). The serum globulin and interleukin-1β, immunoglobulin G concentrations, and catalase activity significantly increased in T2 (P < 0.05). Furthermore, the serum interferon-α level in T1 was significantly higher than that of the CON (P < 0.05). The intestinal barrier-related mRNA gene ZO-1, CLDN1 , and MUC2 expression in the jejunum was significantly upregulated in the T1 and T2 groups (P < 0.05). The Firmicutes/Bacteroidetes ratio was higher and the relative abundances of Flavonifractor and Clostridiales_noname were significantly higher in the T1 group (P < 0.05). In conclusion, dietary supplementation with L. salivarius SNK-6 may improve hen egg quality, serum antioxidant capacity, immune function, and intestinal health. [ABSTRACT FROM AUTHOR]

• Self-assembled tubular g-C 3 N 4 was synthesized via a facile thermal treatment. • g-C 3 N 4 nanotubes were coupled with BiOI microspheres to form Z-scheme heterojunction. • 5 wt%-g-C 3 N 4 /BiOI heterojunction exhibited the highest TC removal efficiency. Tubular g-C 3 N 4 derived from melamine and trithiocyanuric acid was coupled with flower-like BiOI microspheres to form Z-scheme heterojunction photocatalyst for efficient photocatalytic degradation of tetracycline (TC). The structure, morphology, and optical properties of the prepared g-C 3 N 4 /BiOI were characterized. When the mass fractions of g-C 3 N 4 in the composites was 5 wt%, g-C 3 N 4 /BiOI exhibited the highest removal efficiency of 84.2 % within 60 min. The enhanced photocatalytic performance of g-C 3 N 4 /BiOI could be ascribed to stronger absorption in the visible region and faster charge transfer rate. Trapping experiments revealed that h+ and ·O 2 − radicals were the main active species. Based on the band structure and trapping experiments, the Z-scheme pathway was proposed to clarify the photocatalytic mechanism. [ABSTRACT FROM AUTHOR]