17 results on '"Barnes, John"'
Search Results
2. Outbreak of Highly Pathogenic Avian Influenza A(H5N1) Viruses in U.S. Dairy Cattle and Detection of Two Human Cases--United States, 2024
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Garg, Shikha, Reed, Carrie, Davis, C. Todd, Uyeki, Timothy M., Behravesh, Casey Barton, Kniss, Krista, Budd, Alicia, Biggerstaff, Matthew, Adjemian, Jennifer, Barnes, John R., Kirby, Marie K., Basler, Colin, Szablewski, Christine M., Richmond-Crum, Malia, Burns, Erin, Limbago, Brandi, Daskalakis, Demetre C., Armstrong, Kimberly, Boucher, David, Shimabukuro, Tom T., Jhung, Michael A., Olsen, Sonja J., and Dugan, Vivien
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United States. Department of Agriculture ,United States. Food and Drug Administration ,United States. National Institute of Allergy and Infectious Diseases ,Avian influenza -- Health aspects ,Milk -- Health aspects ,Avian influenza viruses -- Health aspects ,Dairy cattle -- Health aspects ,Dairy farming -- Health aspects ,Conjunctivitis -- Health aspects ,Public health -- Health aspects ,Virus diseases -- Health aspects ,Health - Abstract
On May 24, 2024, this report was posted as an MMWR Early Release on the MMWR website (https://www.cdc.gov/mmwr). Investigation and Findings Identification of Two Human Cases of Influenza A(H5) Virus [...]
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- 2024
3. My son taught me to focus on what matters in this digital parenting age; He just wanted me to be as happy watching him sing in his school's concert as he was performing, instead of me being busy filming the event, John Barnes writes
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Barnes, John
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Parenting -- Personal narratives ,Fathers and sons -- Methods ,General interest ,News, opinion and commentary - Abstract
Byline: JOHN BARNES What can we learn from children that we don't already know? As parents, we are supposed to be the leaders, but it doesn't always feel that way. [...]
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- 2024
4. Gospel music brings Black witness to Catholic liturgy
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Barnes, John
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Music, Black -- Appreciation -- Religious aspects ,Concerts -- Appreciation ,Gospel music -- Appreciation ,Philosophy and religion ,Dave Matthews Band (Music group) -- Appreciation - Abstract
GUEST VOICES Recently I attended my first Dave Matthews Band concert. Over the last couple years, I have become an avid listener of DMB as a result of their eclectic [...]
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- 2024
5. The Recovery and Re-Calibration of a 13-Month Aerosol Extinction Profiles Dataset from Searchlight Observations from New Mexico, after the 1963 Agung Eruption.
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Antuña-Marrero, Juan-Carlos, Mann, Graham W., Barnes, John, Calle, Abel, Dhomse, Sandip S., Cachorro, Victoria E., Deshler, Terry, Li, Zhengyao, Sharma, Nimmi, and Elterman, Louis
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STRATOSPHERIC aerosols ,AEROSOLS ,TROPOSPHERIC aerosols ,TROPOSPHERIC ozone ,VOLCANIC eruptions - Abstract
The recovery and re-calibration of a dataset of vertical aerosol extinction profiles of the 1963/64 stratospheric aerosol layer measured by a searchlight at 32° N in New Mexico, US, is reported. The recovered dataset consists of 105 aerosol extinction profiles at 550 nm that cover the period from December 1963 to December 1964. It is a unique record of the portion of the aerosol cloud from the March 1963 Agung volcanic eruption that was transported into the Northern Hemisphere subtropics. The data-recovery methodology involved re-digitizing the 105 original aerosol extinction profiles from individual Figures within a research report, followed by the re-calibration. It involves inverting the original equation used to compute the aerosol extinction profile to retrieve the corresponding normalized detector response profile. The re-calibration of the original aerosol extinction profiles used Rayleigh extinction profiles calculated from local soundings. Rayleigh and aerosol slant transmission corrections are applied using the MODTRAN code in transmission mode. Also, a best-estimate aerosol phase function was calculated from observations and applied to the entire column. The tropospheric aerosol phase function from an AERONET station in the vicinity of the searchlight location was applied between 2.76 to 11.7 km. The stratospheric phase function, applied for a 12.2 to 35.2 km altitude range, is calculated from particle-size distributions measured by a high-altitude aircraft in the vicinity of the searchlight in early 1964. The original error estimate was updated considering unaccounted errors. Both the re-calibrated aerosol extinction profiles and the re-calibrated stratospheric aerosol optical depth magnitudes showed higher magnitudes than the original aerosol extinction profiles and the original stratospheric aerosol optical depth, respectively. However, the magnitudes of the re-calibrated variables show a reasonable agreement with other contemporary observations. The re-calibrated stratospheric aerosol optical depth demonstrated its consistency with the tropics-to-pole decreasing trend, associated with the major volcanic eruption stratospheric aerosol pattern when compared to the time-coincident stratospheric aerosol optical depth lidar observations at Lexington at 42° N. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Late‐Season Influenza Vaccine Effectiveness Against Medically Attended Outpatient Illness, United States, December 2022–April 2023.
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Chung, Jessie R., Shirk, Philip, Gaglani, Manjusha, Mutnal, Manohar B., Nowalk, Mary Patricia, Moehling Geffel, Krissy, House, Stacey L., Curley, Tara, Wernli, Karen J., Kiniry, Erika L., Martin, Emily T., Vaughn, Ivana A., Murugan, Vel, Lim, Efrem S., Saade, Elie, Faryar, Kiran, Williams, Olivia L., Walter, Emmanuel B., Price, Ashley M., and Barnes, John R.
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FLU vaccine efficacy ,INFLUENZA viruses ,INFLUENZA ,VIRUS identification - Abstract
Background: The 2022–23 US influenza season peaked early in fall 2022. Methods: Late‐season influenza vaccine effectiveness (VE) against outpatient, laboratory‐confirmed influenza was calculated among participants of the US Influenza VE Network using a test‐negative design. Results: Of 2561 participants enrolled from December 12, 2022 to April 30, 2023, 91 laboratory‐confirmed influenza cases primarily had A(H1N1)pdm09 (6B.1A.5a.2a.1) or A(H3N2) (3C.2a1b.2a.2b). Overall, VE was 30% (95% confidence interval −9%, 54%); low late‐season activity precluded estimation for most subgroups. Conclusions: 2022–23 late‐season outpatient influenza VE was not statistically significant. Genomic characterization may improve the identification of influenza viruses that circulate postinfluenza peak. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Genetic drift and purifying selection shape within-host influenza A virus populations during natural swine infections.
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VanInsberghe, David, McBride, Dillon S., DaSilva, Juliana, Stark, Thomas J., Lau, Max S. Y., Shepard, Samuel S., Barnes, John R., Bowman, Andrew S., Lowen, Anice C., and Koelle, Katia
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GENETIC drift ,INFLUENZA A virus ,INFLUENZA viruses ,SINGLE nucleotide polymorphisms ,SWINE - Abstract
Patterns of within-host influenza A virus (IAV) diversity and evolution have been described in natural human infections, but these patterns remain poorly characterized in non-human hosts. Elucidating these dynamics is important to better understand IAV biology and the evolutionary processes that govern spillover into humans. Here, we sampled an IAV outbreak in pigs during a week-long county fair to characterize viral diversity and evolution in this important reservoir host. Nasal wipes were collected on a daily basis from all pigs present at the fair, yielding up to 421 samples per day. Subtyping of PCR-positive samples revealed the co-circulation of H1N1 and H3N2 subtype swine IAVs. PCR-positive samples with robust Ct values were deep-sequenced, yielding 506 sequenced samples from a total of 253 pigs. Based on higher-depth re-sequenced data from a subset of these initially sequenced samples (260 samples from 168 pigs), we characterized patterns of within-host IAV genetic diversity and evolution. We find that IAV genetic diversity in single-subtype infected pigs is low, with the majority of intrahost Single Nucleotide Variants (iSNVs) present at frequencies of <10%. The ratio of the number of nonsynonymous to the number of synonymous iSNVs is significantly lower than under the neutral expectation, indicating that purifying selection shapes patterns of within-host viral diversity in swine. The dynamic turnover of iSNVs and their pronounced frequency changes further indicate that genetic drift also plays an important role in shaping IAV populations within pigs. Taken together, our results highlight similarities in patterns of IAV genetic diversity and evolution between humans and swine, including the role of stochastic processes in shaping within-host IAV dynamics. Author summary: Transmission of influenza A viruses among swine occurs commonly at agricultural fairs, where animals from geographically disparate locations come together for a week-long public event. Through dense sampling of one such outbreak, following by viral genome sequencing within positive samples, we evaluated viral evolution within infected pigs. We characterized patterns of viral genetic diversity and investigated the dynamics of variants to understand the forces driving evolutionary change. Our data indicate that influenza virus populations infecting farmed swine have limited genetic diversity and are largely shaped by purifying selection and genetic drift. These observations are similar to those described previously for influenza virus populations within human hosts. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Letters.
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Edge, David, Barnes, John, Hitchins, Ray, Goode, Richard, Harrison, Paul, Wells, Owen, Dyer, Paul, Barlow, Mike, Yih, Tony, Dyer-Godfrey, Paul, and Morgan, Danny
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ELECTRIC bicycles ,CYCLING - Abstract
This article discusses the author's frustrations with the train planning system, highlighting inconsistencies and lack of reliable information. It also touches on the difficulties of planning travel in the modern age, where multiple websites need to be checked for accurate information. The author shares personal anecdotes about cycling and the use of mirrors for safety, as well as their experience with using an electric bike to continue cycling despite leg troubles. The text also briefly mentions various topics related to transportation, such as a bike review, electric car speed limits, and the cost of electricity in Corsica. [Extracted from the article]
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- 2024
9. In‐field detection and characterization of B/Victoria lineage deletion variant viruses causing early influenza activity and an outbreak in Louisiana, 2019.
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Shu, Bo, Wilson, Malania M., Keller, Matthew W., Tran, Ha, Sokol, Theresa, Lee, Grace, Rambo‐Martin, Benjamin L., Kirby, Marie K., Hassell, Norman, Haydel, Danielle, Hand, Julie, Wentworth, David E., and Barnes, John R.
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COVID-19 ,INFLUENZA ,VIRAL vaccines ,AMINO acids ,HEMAGGLUTININ ,REVERSE transcriptase polymerase chain reaction - Abstract
Background: In 2019, the Louisiana Department of Health reported an early influenza B/Victoria (B/VIC) virus outbreak. Method: As it was an atypically large outbreak, we deployed to Louisiana to investigate it using genomics and a triplex real‐time RT‐PCR assay to detect three antigenically distinct B/VIC lineage variant viruses. Results: The investigation indicated that B/VIC V1A.3 subclade, containing a three amino acid deletion in the hemagglutinin and known to be antigenically distinct to the B/Colorado/06/2017 vaccine virus, was the most prevalent circulating virus within the specimens evaluated (86/88 in real‐time RT‐PCR). Conclusion: This work underscores the value of portable platforms for rapid, onsite pathogen characterization. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Reader Corner.
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MORRIS, STACEY, BUZBY, AMY, BARNES, JOHN, JUNKKARI, MARKKU, and BAISSAC, CLAUDE
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FORUMS - Abstract
The article focuses on a Times reader challenge to spend 10 minutes staring at a painting, revealing that while only 25 percent completed the task, many shared insightful and varied personal reflections on the experience.
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- 2024
11. First Report of Xanthomonas euvesicatoria pv. sesami Causing Leaf Spot on Sesame ( Sesamum indicum L.) in South Carolina, USA.
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Noh E, Panth M, Yang X, Barnes JM, and Wang H
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Sesame (Sesamum indicum L.) is an annual plant known as one of the first domesticated oilseed crops. It is cultivated worldwide, mostly in Asia, Africa, and the Americas (Singh, 2006). In August 2022 and September 2023, dark angular necrotic spots on leaves and stems (100% incidence), blights, and severe defoliation were observed in a 4-acre rainfed sesame field located in the Colleton County of South Carolina, USA (Fig. S1). Bacterial streaming from cut leaf lesions was observed from diseased plants in both years. Two plants were collected for pathogen isolation in 2023. Symptomatic leaves were surface sterilized with 70% ethanol for 1 min and dried in a laminar flow hood. For each isolate, four sterile toothpicks were used to poke lesion margins and stirred in 300 µl of sterile distilled water in a 2-ml sterile microcentrifuge tube and soaked at room temperature (c. 21 °C) for 10 min. Each bacterial suspension (10 µl) was streaked on nutrient agar (NA) in a Petri dish. Convex and mucoid yellow colonies formed after a 48-h incubation at 28°C in the dark. Two isolates (S813 and S814), one from each plant, were obtained by transferring single colonies to new NA plates. Both isolates were preliminarily identified as Xanthomonas [S813: X. campestris (P = 0.53); S814: X. campestris (P = 0.77)] using a Biolog Microbial Identification System (GEN III Microplate; Identification Database v.2.8.0.15G). PCR amplification of the atpD and dnaK genes was performed for both isolates using the conditions described in Félix-Gastélum et al. (2019). The sequences of both amplicons are 100% identical for each gene between the two isolates. PCR and sequencing of the gyrB gene was also done for S813 with the primers from Young et al. (2008). The atpD (S813/S814), dnaK (S813/S814), and gyrB (S813) sequences (GenBank accessions: PP507118 to PP507120) showed the best match with 100% identity to the corresponding gene sequences [GenBank accessions: KJ491167 (100% coverage), KJ491257 (99% coverage), EU285201 (100% coverage)] of the X. euvesicatoria pv. sesami (=X. campestris pv. sesami) type strain LMG865 (Constantin et al. 2015, Parkinson et al. 2009). A neighbor joining tree with the concatenated sequences of these three genes (2,210 nt) showed that S813 and LMG 865 had the closet relationship with X. euvesicatoria pv. alfalfae (CFBP3836, Fig. S2). To fulfill Koch's postulates, three healthy sesame plants (cultivar Shirogoma) were spray inoculated separately with each suspension of S813 and S814 in sterile tap water until runoff (approx. 5×108 CFU/ml). Two sesame plants were sprayed with sterile tap water and served as negative control. All plants were maintained in a greenhouse at approximately 28/20°C (day/night) with natural photoperiod. Dark leaf spots and leaf yellowing were observed on inoculated plants 7 to 14 days after inoculation. No disease symptom was observed on the control plants. Bacteria were reisolated from leaf spots of the inoculated plants and confirmed to be X. euvesicatoria pv. sesami based on atpD and dnaK sequences. The disease was first reported in Sudan (Sabet and Dowson, 1960), after which it was reported in USA (Isakeit et al., 2012) and Mexico (Félix-Gastélum et al. 2019). To the best of our knowledge, this is the first report of this disease in South Carolina, USA. Since the interest of sesame to the farmers is increasing in the southeastern USA, it is necessary to perform further research to examine the disease distribution and its economic impact.
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- 2024
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12. Highly Pathogenic Avian Influenza A(H5N1) Virus Infection in a Dairy Farm Worker.
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Uyeki TM, Milton S, Abdul Hamid C, Reinoso Webb C, Presley SM, Shetty V, Rollo SN, Martinez DL, Rai S, Gonzales ER, Kniss KL, Jang Y, Frederick JC, De La Cruz JA, Liddell J, Di H, Kirby MK, Barnes JR, and Davis CT
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- Adult, Animals, Female, Humans, Male, Middle Aged, Farmers, United States epidemiology, Dairying statistics & numerical data, Influenza A Virus, H5N1 Subtype isolation & purification, Influenza in Birds epidemiology, Influenza in Birds transmission, Influenza in Birds virology, Influenza, Human diagnosis, Influenza, Human epidemiology, Influenza, Human transmission, Influenza, Human virology, Milk virology, Occupational Exposure
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- 2024
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13. Outbreak of Highly Pathogenic Avian Influenza A(H5N1) Viruses in U.S. Dairy Cattle and Detection of Two Human Cases - United States, 2024.
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Garg S, Reed C, Davis CT, Uyeki TM, Behravesh CB, Kniss K, Budd A, Biggerstaff M, Adjemian J, Barnes JR, Kirby MK, Basler C, Szablewski CM, Richmond-Crum M, Burns E, Limbago B, Daskalakis DC, Armstrong K, Boucher D, Shimabukuro TT, Jhung MA, Olsen SJ, and Dugan V
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- Animals, Cattle, Humans, Cattle Diseases epidemiology, United States epidemiology, Disease Outbreaks, Influenza A Virus, H5N1 Subtype isolation & purification, Influenza, Human epidemiology
- Abstract
Competing Interests: All authors have completed and submitted the International Committee of Medical Journal Editors form for disclosure of potential conflicts of interest. No potential conflicts of interest were disclosed.
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- 2024
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14. Antigenic drift and subtype interference shape A(H3N2) epidemic dynamics in the United States.
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Perofsky AC, Huddleston J, Hansen C, Barnes JR, Rowe T, Xu X, Kondor R, Wentworth DE, Lewis N, Whittaker L, Ermetal B, Harvey R, Galiano M, Daniels RS, McCauley JW, Fujisaki S, Nakamura K, Kishida N, Watanabe S, Hasegawa H, Sullivan SG, Barr IG, Subbarao K, Krammer F, Bedford T, and Viboud C
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Influenza viruses continually evolve new antigenic variants, through mutations in epitopes of their major surface proteins, hemagglutinin (HA) and neuraminidase (NA). Antigenic drift potentiates the reinfection of previously infected individuals, but the contribution of this process to variability in annual epidemics is not well understood. Here we link influenza A(H3N2) virus evolution to regional epidemic dynamics in the United States during 1997-2019. We integrate phenotypic measures of HA antigenic drift and sequence-based measures of HA and NA fitness to infer antigenic and genetic distances between viruses circulating in successive seasons. We estimate the magnitude, severity, timing, transmission rate, age-specific patterns, and subtype dominance of each regional outbreak and find that genetic distance based on broad sets of epitope sites is the strongest evolutionary predictor of A(H3N2) virus epidemiology. Increased HA and NA epitope distance between seasons correlates with larger, more intense epidemics, higher transmission, greater A(H3N2) subtype dominance, and a greater proportion of cases in adults relative to children, consistent with increased population susceptibility. Based on random forest models, A(H1N1) incidence impacts A(H3N2) epidemics to a greater extent than viral evolution, suggesting that subtype interference is a major driver of influenza A virus infection dynamics, presumably via heterosubtypic cross-immunity., Competing Interests: Competing interests The WHO Collaborating Centre for Reference and Research on Influenza in Melbourne has a collaborative research and development agreement (CRADA) with CSL Seqirus for isolation of candidate vaccine viruses in cells and an agreement with IFPMA for isolation of candidate vaccine viruses in eggs. SGS reports honoraria from CSL Seqirus, Moderna, Pfizer, and Evo Health. The Icahn School of Medicine at Mount Sinai has filed patent applications relating to influenza virus vaccines, SARS-CoV-2 serological assays, and SARS-CoV-2 vaccines which list FK as co-inventor. Mount Sinai has spun out companies, Kantaro and Castlevax, to market the SARS-CoV-2 related technologies. FK has consulted for Merck and Pfizer (before 2020), and is currently consulting for Pfizer, Seqirus, 3rd Rock Ventures, GSK and Avimex. The Krammer laboratory is also collaborating with Pfizer on animal models of SARS-CoV-2 and with Dynavax on universal influenza virus vaccines. All other authors declare no competing interests.
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- 2024
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15. Antigenic Characterization of Circulating and Emerging SARS-CoV-2 Variants in the U.S. throughout the Delta to Omicron Waves.
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Di H, Pusch EA, Jones J, Kovacs NA, Hassell N, Sheth M, Lynn KS, Keller MW, Wilson MM, Keong LM, Cui D, Park SH, Chau R, Lacek KA, Liddell JD, Kirby MK, Yang G, Johnson M, Thor S, Zanders N, Feng C, Surie D, DeCuir J, Lester SN, Atherton L, Hicks H, Tamin A, Harcourt JL, Coughlin MM, Self WH, Rhoads JP, Gibbs KW, Hager DN, Shapiro NI, Exline MC, Lauring AS, Rambo-Martin B, Paden CR, Kondor RJ, Lee JS, Barnes JR, Thornburg NJ, Zhou B, Wentworth DE, and Davis CT
- Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has evolved into numerous lineages with unique spike mutations and caused multiple epidemics domestically and globally. Although COVID-19 vaccines are available, new variants with the capacity for immune evasion continue to emerge. To understand and characterize the evolution of circulating SARS-CoV-2 variants in the U.S., the Centers for Disease Control and Prevention (CDC) initiated the National SARS-CoV-2 Strain Surveillance (NS3) program and has received thousands of SARS-CoV-2 clinical specimens from across the nation as part of a genotype to phenotype characterization process. Focus reduction neutralization with various antisera was used to antigenically characterize 143 SARS-CoV-2 Delta, Mu and Omicron subvariants from selected clinical specimens received between May 2021 and February 2023, representing a total of 59 unique spike protein sequences. BA.4/5 subvariants BU.1, BQ.1.1, CR.1.1, CQ.2 and BA.4/5 + D420N + K444T; BA.2.75 subvariants BM.4.1.1, BA.2.75.2, CV.1; and recombinant Omicron variants XBF, XBB.1, XBB.1.5 showed the greatest escape from neutralizing antibodies when analyzed against post third-dose original monovalent vaccinee sera. Post fourth-dose bivalent vaccinee sera provided better protection against those subvariants, but substantial reductions in neutralization titers were still observed, especially among BA.4/5 subvariants with both an N-terminal domain (NTD) deletion and receptor binding domain (RBD) substitutions K444M + N460K and recombinant Omicron variants. This analysis demonstrated a framework for long-term systematic genotype to antigenic characterization of circulating and emerging SARS-CoV-2 variants in the U.S., which is critical to assessing their potential impact on the effectiveness of current vaccines and antigen recommendations for future updates.
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- 2024
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16. Targeted amplification and genetic sequencing of the severe acute respiratory syndrome coronavirus 2 surface glycoprotein.
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Keller MW, Keong LM, Rambo-Martin BL, Hassell N, Lacek KA, Wilson MM, Kirby MK, Liddell J, Owuor DC, Sheth M, Madden J, Lee JS, Kondor RJ, Wentworth DE, and Barnes JR
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- Humans, SARS-CoV-2 genetics, Pandemics, Membrane Glycoproteins, COVID-19 epidemiology, Vaccines
- Abstract
Importance: The COVID-19 pandemic was accompanied by an unprecedented surveillance effort. The resulting data were and will continue to be critical for surveillance and control of SARS-CoV-2. However, some genomic surveillance methods experienced challenges as the virus evolved, resulting in incomplete and poor quality data. Complete and quality coverage, especially of the S-gene, is important for supporting the selection of vaccine candidates. As such, we developed a robust method to target the S-gene for amplification and sequencing. By focusing on the S-gene and imposing strict coverage and quality metrics, we hope to increase the quality of surveillance data for this continually evolving gene. Our technique is currently being deployed globally to partner laboratories, and public health representatives from 79 countries have received hands-on training and support. Expanding access to quality surveillance methods will undoubtedly lead to earlier detection of novel variants and better inform vaccine strain selection., Competing Interests: The authors declare no conflict of interest.
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- 2024
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17. Author Correction: Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants.
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Welch NL, Zhu M, Hua C, Weller J, Mirhashemi ME, Nguyen TG, Mantena S, Bauer MR, Shaw BM, Ackerman CM, Thakku SG, Tse MW, Kehe J, Uwera MM, Eversley JS, Bielwaski DA, McGrath G, Braidt J, Johnson J, Cerrato F, Moreno GK, Krasilnikova LA, Petros BA, Gionet GL, King E, Huard RC, Jalbert SK, Cleary ML, Fitzgerald NA, Gabriel SB, Gallagher GR, Smole SC, Madoff LC, Brown CM, Keller MW, Wilson MM, Kirby MK, Barnes JR, Park DJ, Siddle KJ, Happi CT, Hung DT, Springer M, MacInnis BL, Lemieux JE, Rosenberg E, Branda JA, Blainey PC, Sabeti PC, and Myhrvold C
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- 2024
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