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[Display omitted] • Drug-in-cyclodextrin-in-liposome (DCL) was used to encapsulated limonene (Lim). • Complexation ratio of 1:1 of Lim/HP-β-CD complex was analyzed by molecular dynamics simulation. • The average size was 147.5 nm, and zeta potential was 48.7 mv. • DCL formulation improved the stability and enhanced the antioxidant activity of Lim. • DCL can be used as a nanocarrier of food matrix for volatile and hydrophobic compounds. Drug-in-cyclodextrin-in-liposome (DCL) combines advantages of cyclodextrin and liposome. Here, DCL formulation was successfully prepared to encapsulate limonene (Lim), whose characterization revealed that particle size was 147.5 ± 1.3 nm and zeta potential was −48.7 ± 0.8 mV. And the complexation mechanism of Lim/HP-β-CD inclusion complex (the intermediate of DCL) was analyzed by molecular dynamics simulation, showing that Lim was entrapped into the cavity of HP-β-CD through electrostatic and hydrophobic interaction with a molar ratio of 1:1. Notably, DCL formulation not only reduced Lim volatilization in 25℃, but also enhanced the free radical (DPPH· and ABTS·+) scavenging ability of Lim. In summary, Lim-DCL formulation improved the stability and enhanced the antioxidant activity of Lim. DCL nanocarrier system is suitable to preserve volatile and hydrophobic compounds, enlarging their application in pharmaceutics industries. [ABSTRACT FROM AUTHOR]

The use of pooled data from different repeated survey series to study long-term trends is handicapped by a measurement difficulty: different survey series often use different scales to measure the same attitude and thus generate scale-incomparable data. In this article, the authors propose the latent attitude method (LAM) to address this scale-incomparability problem, on the basis of the assumption that attitudes measured by ordinal categories reflect a latent attitude with cut points. The method extends the latent variable method in the case of a single survey series to the case of multiple survey series and leverages overlapping years for identification. The authors first assess the validity of the method with simulated data. The results show that the method yields accurate estimates of mean attitudes and cut point values. The authors then apply the method to an empirical study of Americans' attitudes toward China from 1974 to 2019. [ABSTRACT FROM AUTHOR]

The decline in vascular capacity within the meniscus is a well-documented phenomenon during both development and degeneration. Maintaining vascular integrity has been proposed as a potential therapeutic strategy for osteoarthritis. Therefore, our study aims to investigate the characteristics of endothelial cells and blood vessels in embryonic and degenerated meniscus tissues. Human embryonic and mature menisci were used for histological analyses. Single-cell RNA sequencing was used to identify cell clusters and their significant genes in embryo meniscus to uncover characteristic of endothelial cells. Computer analysis and various staining techniques were used to characterize vessels in development and osteoarthritis meniscus. Vessels structure first observed in E12w and increasing in E14w. Vessels were veins majorly and arteries growth in E35w. Endothelial cells located not only perivascular but also in the surface of meniscus. The expression of DLL1 was observed to be significantly altered in endothelial cells within the vascular network that failed to form. Meniscus tissues affected by osteoarthritis, characterized by diminished vascular capacity, displayed reduced levels of DLL1 expression. Experiment in vitro confirmed DLL1/NOTCH1 be vital to angiogenesis. Lack of DLL1/NOTCH1 signaling pathway was mechanism of vascular declination in development and degenerated meniscus. [ABSTRACT FROM AUTHOR]