1. CXCR4 positive bone mesenchymal stem cells migrate to human endothelial cell stimulated by ox-LDL via SDF-1α/CXCR4 signaling axis
- Author
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Li, Mincai, Yu, Jun, Li, Yan, Li, Dujuan, Yan, Dan, Qu, Zhiling, and Ruan, Qiurong
- Subjects
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STEM cells , *CELL migration , *BONE cells , *ENDOTHELIUM , *CELLULAR signal transduction , *MEDICAL genetics , *CARDIOVASCULAR diseases , *CHEMOTAXIS , *ATHEROSCLEROSIS - Abstract
Abstract: Background: Bone mesenchymal stem cells (BMSCs) are attractive candidates for cell based therapies to cardiovascular disease such as infarction and atherosclerosis; however, the mechanisms responsible for stem cell chemotaxis and homing remain unknown. Chemokine stromal cell-derived factor 1 (SDF-1α) is involved in the process of atherogenesis. This study was aimed at investigating whether the SDF-1α of human umbilical vein endothelial cells (HUVECs) plays a role in migration of BM-derived CXCR4+(receptor for SDF-1α) stem cells. Methods: HUVECs were cultured from human umbilical cords and was treated with ox-LDL. The mRNA and protein expression of SDF-1α was detected in HUVECs. CXCR4+BMSCs from bone marrow were isolated and were tested by migration and adhesion assays. Results: It was found that ox-LDL induced HUVECs to increase the mRNA and protein expression of SDF-1α. Ox-LDL increased the migratory and adhesion response of CXCR4+BMSCs. When the neutralizing SDF-1α antibody abrogated the secreted SDF-1α, the migration and adhesion response of CXCR4+BMSCs markedly decreased. Conclusions: Our data indicated that the endothelial cells (ECs) stimulated by ox-LDL could increase the BMSCs migratory response via SDF-1α/CXCR4 signaling axis. These findings provide a new paradigm for biological effects of ox-LDL and have implications for novel stem cell therapeutic strategies for atherosclerosis. [Copyright &y& Elsevier]
- Published
- 2010
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