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Plant gray mold disease caused by necrotrophic pathogen Botrytis cinerea , has been a serious threat to many important crops. It is urgent and necessary to seek an effective and environmentally friendly control strategy to control grey mold disease during the crop production. Moreover, it is also urgently needed to clear understand how the strategy works. To screening an efficient biocontrol agent in control of gray mold disease caused by B. cinerea , one hundred strains, which have the potential in controlling plant diseases, were selected from our laboratory’s strain library, and employed to our research. The results in the study indicated that Bacillus velezensis could act as a potential and efficient biocontrol agent (BCA), and two B. velezensis strains 5YN8 and DSN012 were screened out with high antagonistic and hydrolase activity, which could significantly control pepper gray mold disease and promote the pepper growth. Further deeply study on biocontrol mechanism indicated that B. velezensis could suppress the growth and spore formation of B. cinerea by secreting some secondary metabolites or releasing numbers of volatile organic compounds (VOCs). In the other hand, we also found that B. velezensis could trigger plant basal immunity. The BCA could increase the expression of SA signal markers and plant defense related genes. It was able to induce hydrogen peroxide accumulation and plant related enzyme activities in leaves of pepper as well. This study provides another high-efficiency, and potential strategies for the biological control of gray mold disease. It is the first report of B. velezensis strain which can control gray mold disease, In addition, it is also the first to comprehensively illustrate how the strains can efficiently protect pepper from B. cinerea attacking. [ABSTRACT FROM AUTHOR]