Search

Bivalve lectins perform a crucial function in recognition of foreign particles, such as microalgae and pathogenic bacteria. In this study, a novel C-type lectin form Sinonovacula constricta (ScCL) was characterized. The full-length cDNA of ScCL was 1645 bp, encoding a predicted polypeptide of 273 amino acids with one typical carbohydrate-recognition domain. ScCL has the highest similarity and closest phylogenetic relationship with the C-type lectin from Solen grandis. Real-time PCR analysis showed that ScCL was expressed in all tested tissues, with the highest expression in the foot and the lowest expression in hemocytes. Agglutination activity of ScCL was Ca2+-independent. ScCL showed the strongest agglutination on Chlorella vulgaris , the modest agglutination on Platymonas subcordiformis , Nannochloropsis sp., and Thalassiosira pseudonana , the weakest agglutination on Chaetoceros sp., and no agglutination on Isochrysis zhanjiangensis. Meanwhile, agglutination tests and western blot analysis revealed that the recombinant ScCL protein could agglutinate Staphylococcus aureus and Vibrio harveyi , but could not agglutinate Vibrio anguillarum , Bacillus cereus , or Vibrio parahaemolyticus. Furthermore, ScCL had a high binding activity with LPS and mannose, a low binding activity with LTA, and no binding activity with PGN. The expression of ScCL in the gill of S. constricta fed with C. vulgaris and T. pseudonana was significantly increased at 1 and/or 3 h. After injection with S. aureus , the expression of ScCL in the gill was significantly increased at 3, 6, and 24 h. These results indicated that ScCL was involved in food particle recognition and immunity of S. constricta. • A novel C-type lectin from Sinonovacula constricta (ScCL) was characterized. • ScCL protein could bind to several PAMPs and bacteria. • ScCL protein could agglutinate several microalgae and bacteria in Ca2+-independent. • After feeding Chlorella vulgaris and Thalassiosira pseudonana , or after challenged with staphylococcus aureus , the expression of ScCL was up-regulated. • ScCL may be involved in food recognition and innate immunity. [ABSTRACT FROM AUTHOR]