1. Overexpressed MPS-1 contributes to endometrioma development through the NF-κB signaling pathway
- Author
-
Xiaohua Liu, Liqi Zhang, Junyan Ma, Jun Lin, and Yang Liu
- Subjects
0301 basic medicine ,Adult ,Stromal cell ,QH471-489 ,Endometriosis ,Gene Expression ,Cell Cycle Proteins ,Protein Serine-Threonine Kinases ,03 medical and health sciences ,Endometrium ,Young Adult ,0302 clinical medicine ,Endocrinology ,Downregulation and upregulation ,Medicine ,Humans ,Endometrioma ,NF-κB signaling pathway ,Cells, Cultured ,Gene knockdown ,business.industry ,Reproduction ,Research ,Ectopic endometrial stromal cells ,NF-kappa B ,Obstetrics and Gynecology ,Gynecology and obstetrics ,Cell cycle ,Protein-Tyrosine Kinases ,medicine.disease ,Blot ,Metallopanstimulin-1 (MPS-1) ,030104 developmental biology ,Reproductive Medicine ,Apoptosis ,030220 oncology & carcinogenesis ,RG1-991 ,Cancer research ,Female ,Signal transduction ,business ,Developmental Biology ,Signal Transduction - Abstract
Background Endometriosis is a benign gynecological disease that shares some characteristics with malignant tumors and affects approximately 10% of women of reproductive age. Endometrioma refers to endometriosis that appears in the ovary. Metallopanstimulin-1 (MPS-1) is a component of the 40S subunit of ribosomes that has extra-ribosomal functions that contribute to the development of diseases. This study aimed to explore the expression pattern and role of MPS-1 in endometrioma development. Methods Quantitative real time polymerase chain reaction, western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay were used to determine the expression of MPS-1 in patients with endometrioma. Following the successful knockdown of MPS-1 by siRNA, CCK-8 assays, flow cytometry, and transwell assays were performed to detect ectopic endometrial stromal cells (EcESCs) proliferation, the rate of apoptosis, and cell cycle, migration, and invasion, respectively. Western blotting was used to explore the effect of MPS-1 knockdown on protein levels in the NF-κB signaling pathway. Results The expression of MPS-1 was significantly higher in endometrioma and the serum of endometrioma patients than in the patients without endometriosis. In addition, the downregulation of MPS-1 expression inhibited EcESCs proliferation, migration, and invasion. This downregulation led to the arrest of the EcESCs cycle in the G0/G1 phase and apoptosis and depressed the NF-κB signaling pathway. Conclusion MPS-1 can regulate EcESCs proliferation, motility, invasion, apoptosis, and cell cycle via the NF-κB signaling pathway in endometrioma. This may contribute to the formation or development of endometriotic foci. This study suggests the potential role of MPS-1 in the pathogenesis of endometriosis and enabled further research into the use of MPS-1 in the clinical diagnosis of endometrioma.
- Published
- 2021