27 results on '"Hong, Guo"'
Search Results
2. Whole-exome sequencing enables rapid and prenatal diagnosis of inherited skin disorders
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Xintong, Zhu, Kexin, Zhang, Junwen, Wang, Ziyi, Wang, Na, Luo, and Hong, Guo
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- 2023
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3. Efficacy and safety of pomalidomide and low-dose dexamethasone in Chinese patients with relapsed or refractory multiple myeloma: a multicenter, prospective, single-arm, phase 2 trial
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Fu, Wei-Jun, Wang, Ya-Fei, Zhao, Hong-Guo, Niu, Ting, Fang, Bai-Jun, Liao, Ai-Jun, Bai, Hai, and Lu, Jin
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- 2022
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4. Long‑range GABAergic projections from the nucleus of the solitary tract.
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Mei‑Yu Shi, Lu‑Feng Ding, Yu‑Hong Guo, Yu‑Xiao Cheng, Guo‑Qiang Bi, and Pak‑Ming Lau
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SOLITARY nucleus ,GLUTAMATE decarboxylase ,CHOLERA toxin ,PARAVENTRICULAR nucleus ,THREE-dimensional imaging ,RHOMBENCEPHALON - Abstract
The nucleus of the solitary tract (NTS) plays a crucial role in integrating peripheral information regarding visceral functions. Glutamate decarboxylase 2 (GAD2) inhibitory neurons are abundant in the NTS, and are known to form local and short-range projections within the NTS and nearby hindbrain areas. Here we performed whole-brain mapping of outputs from GAD2 neurons in the NTS using cell-type specific viral labeling together with ultrahigh-speed 3D imaging at 1-μm resolution. In addition to well-known targets of NTS GAD2 neurons including the principle sensory nucleus of the trigeminal (PSV), spinal nucleus of the trigeminal (SPV), and other short-range targets within the hindbrain, the high sensitivity of our system helps reveal previously unknown long-range projections that target forebrain regions, including the bed nuclei of the stria terminalis (BST) involved in stress and fear responses, and the paraventricular hypothalamic nucleus (PVH) involved in energy balance and stress-related neuroendocrine responses. The long-range projections were further verified by retrograde labeling of NTS GAD2 neurons with cholera toxin B (CTB) injections in the BST and PVH, and by Cre-dependent retrograde tracing with rAAV2-retro injections in the two regions of GAD2-Cre mice. Finally, we performed complete morphological reconstruction of several sparsely labeled neurons projecting to the forebrain and midbrain. These results provide new insights about how NTS might participate in physiological and emotional modulation. [ABSTRACT FROM AUTHOR]
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- 2021
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5. A report of nine cases and review of the literature of infertile men carrying balanced translocations involving chromosome 5.
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Zhang, Hong-Guo, Wang, Rui-Xue, Pan, Yuan, Zhang, Han, Li, Lei-Lei, Zhu, Hai-Bo, and Liu, Rui-Zhi
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MALE infertility , *CHROMOSOMAL translocation , *SPERMATOGENESIS , *GENETIC carriers , *MISCARRIAGE , *GENETICS - Abstract
Background: Balanced translocations may cause the loss of genetic material at the breakpoints and may result in failure of spermatogenesis. However, carriers of reciprocal translocation may naturally conceive. Genetic counseling of male carriers of translocations remains challenging. This study explores the clinical features of carriers of chromosome 5 translocations, enabling informed genetic counseling of these patients. Results: Of 82 translocation carriers, 9 (11%) were carriers of a chromosome 5 translocation. One case had azoospermia, while three cases had experienced recurrent spontaneous abortions, two cases had each experienced stillbirth, and three cases produced a phenotypically normal child confirmed by amniocentesis. A literature review identified 106 patients who carried chromosome 5 translocations. The most common chromosome 5 translocation was t(4,5), observed in 13 patients. Breakpoint at 5p15 was observed in 11 patients. All breakpoints at chromosome 5 were associated with gestational infertility. Conclusion: In genetic counseling, physicians should consider chromosome 5 and its breakpoints. Carriers of chromosome 5 translocations may continue with natural conception or use assisted reproductive technologies, such as preimplantation genetic diagnosis. [ABSTRACT FROM AUTHOR]
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- 2018
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6. Succinyl-proteome profiling of Dendrobium officinale, an important traditional Chinese orchid herb, revealed involvement of succinylation in the glycolysis pathway.
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Shangguo Feng, Kaili Jiao, Hong Guo, Mengyi Jiang, Juan Hao, Huizhong Wang, and Chenjia Shen
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GLYCOLYSIS ,LYSINE ,PROKARYOTIC genomes ,GENE ontology ,HERBS - Abstract
Background: Lysine succinylation is a ubiquitous and important protein post-translational modification in various eukaryotic and prokaryotic cells. However, its functions in Dendrobium officinale, an important traditional Chinese orchid herb with high polysaccharide contents, are largely unknown. Results: In our study, LC-MS/MS was used to identify the peptides that were enriched by immune-purification with a high-efficiency succinyl-lysine antibody. In total, 314 lysine succinylation sites in 207 proteins were identified. A gene ontology analysis showed that these proteins are associated with a wide range of cellular functions, from metabolic processes to stimuli responses. Moreover, two types of conserved succinylation motifs, '***K
suc ******K**' and '****EKsuc ***', were identified. Our data showed that lysine succinylation occurred on five key enzymes in the glycolysis pathway. The numbers of average succinylation sites on these five enzymes in plants were lower than those in bacteria and mammals. Interestingly, two active site amino acids residues, K103 and K225, could be succinylated in fructose-bisphosphate aldolase, indicating a potential function of lysine succinylation in the regulation of glycolytic enzyme activities. Furthermore, the protein-protein interaction network for the succinylated proteins showed that several functional terms, such as glycolysis, TCA cycle, oxidative phosphorylation and ribosome, are consisted. Conclusions: Our results provide the first comprehensive view of the succinylome of D. officinale and may accelerate future biological investigations of succinylation in the synthesis of polysaccharides, which are major active ingredients. [ABSTRACT FROM AUTHOR]- Published
- 2017
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7. Neuroprotective effect of salvianolate lyophilized injection against cerebral ischemia in type 1 diabetic rats.
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Fujiang Wang, Qiansong He, Jinxin Wang, Qing Yuan, Hong Guo, Lijuan Chai, Shaoxia Wang, Limin Hu, and Yue Zhang
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TYPE 1 diabetes ,ANIMAL experimentation ,CEREBRAL ischemia ,FREEZE-drying ,INJECTIONS ,RATS ,STROKE ,NEUROPROTECTIVE agents ,DIAGNOSIS - Abstract
Background: Salvianolate lyophilized injection (SLI) has been clinically used in China for the treatment of acutely cerebral infarction. Clinical and experimental studies have shown that Diabetes mellitus (DM) not only increases the risk of ischemic stroke recurrence but also leads to poor outcomes and increases fatality rates after stroke. Our previous study has proved that SLI can reduce the infarct volume after stroke in type 1 diabetic rats. The aim of the study is to explore the mechanism of SLI on stroke outcome in type 1 diabetic (T1DM) rats. Methods: Type 1 diabetes rats model (T1DM) was induced in male Wistar rats by intraperitoneal (i.p) injection of streptozotocin (60 mg/kg) and T1 DM rats were subjected to intraluminal middle cerebral artery occlusion (MCAO). The T1DM + MCAO rats were randomly divided into six groups: sham-operated, model-vehicle, positive control group (Edaravone-treating, DE 6 mg/kg) and SLI-treating group (10.5 mg/kg, 21 mg/kg and 42 mg/kg). SLI and DE were administered by tail vein injection at 3 h after MCAO, then daily for 14 days. Micro-CT scans of the brain tissue revealed vessel characteristics and distribution in the ischemia zone. Glucose uptake was analyzed by PET/CT. RAGE, MMP9 and inflammatory factors (COX-2, TNF-α and ICAM-1), HQ-1, HQO-1 and Nrf-2 expression levels in the ischemic brain tissue were analyzed by Immunofluorescence staining and Western blot at 14 days after MCAO. Results: In this study, we have demonstrated that SLI treatment significantly increased the number of brain microvasculature in ipsilateral and glucose uptake in cortex, hippocampus and penumbra in the T1DM + MCAO rats. SLI also significantly decreased the expression of RAGE, MMP9 and inflammatory factors expression, and increased the expression of HQ-1, HQO-1 and Nrf-2 in T1DM + MCAO rats. Conclusion: The study showed that SLI could protect against cerebral ischemia injury in T1DM + MCAO rats and the mechanism is related to decrease inflammatory factors and activate of the Nrf2/HO-1 signaling pathway. [ABSTRACT FROM AUTHOR]
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- 2017
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8. Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus.
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Yongil Yang, Chang Geun Yoo, Hao-Bo Guo, Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Gunter, Lee E., Jawdy, Sara S., Xiaohan Yang, Hong Guo, Yunqiao Pu, Ragauskas, Arthur J., Tuskan, Gerald A., and Jin-Gui Chen
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PLANT growth ,ENERGY crops ,CELLULOSE ,GENETIC overexpression ,PLANT proteins - Abstract
Background: Domain of Unknown Function 266 (DUF266) is a plant-specific domain. DUF266-containing proteins (DUF266 proteins) have been categorized as 'not classified glycosyltransferases (GTnc)' due to amino acid similarity with GTs. However, little is known about the function of DUF266 proteins. Results: Phylogenetic analysis revealed that DUF266 proteins are only present in the land plants including moss and lycophyte. We report the functional characterization of one member of DUF266 proteins in Populus, PdDUF266A. PdDUF266A was ubiquitously expressed with high abundance in the xylem. In Populus transgenic plants overexpressing PdDUF266A (OXPdDUF266A), the glucose and cellulose contents were significantly higher, while the lignin content was lower than that in the wild type. Degree of polymerization of cellulose in OXPdDUF266A transgenic plants was also higher, whereas cellulose crystallinity index remained unchanged. Gene expression analysis indicated that cellulose biosynthesis-related genes such as CESA and SUSY were upregulated in mature leaf and xylem of OXPdDUF266A transgenic plants. Moreover, PdDUF266A overexpression resulted in an increase of biomass production. Their glucose contents and biomass phenotypes were further validated via heterologous expression of PdDUF266A in Arabidopsis. Results from saccharification treatment demonstrated that the rate of sugar release was increased by approximately 38% in the OXPdDUF266A transgenic plants. Conclusions: These results suggest that the overexpression of PdDUF266A can increase cellulose content, reduce recalcitrance, and enhance biomass production, and that PdDUF266A is a promising target for genetic manipulation for biofuel production. [ABSTRACT FROM AUTHOR]
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- 2017
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9. Genome-wide investigation and expression analyses of the pentatricopeptide repeat protein gene family in foxtail millet.
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Jia-Ming Liu, Zhao-Shi Xu, Pan-Pan Lu, Wei-Wei Li, Ming Chen, Chang-Hong Guo, and You-Zhi Ma
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PENTATRICOPEPTIDE repeat genes ,FOXTAIL millet ,GENE expression ,ARABIDOPSIS ,PHYLOGENY ,PLASTIDS ,ABIOTIC stress - Abstract
Background: Pentatricopeptide repeat (PPR) proteins are encoded by a large gene family of approximately 450 members in Arabidopsis and 477 in rice, which characterized by tandem repetitions of a degenerate 35 amino acid characteristic sequence motifs. A large majority of the PPR genes in the higher plants are localized in organelles. Their functions remain as yet largely unknown. The majority of characterized PPR proteins have been found to function in modulating the expression plastid and mitochondrial genes in plants. Results: Here, a genome-wide identification and comparison of the PPR genes from 5 organisms was performed, including the moss Physcomitrella patens, the lycophyte Selaginella moellendorffii, the eudicot Arabidopsis, and the monocots rice and foxtail millet. It appears that the expansion of this gene family prior to the divergence of the euphyllophytes and the lycophytes in land plants. The duplication and divergence rates of the foxtail millet PPR genes (SiPPRs) showed that the expansion period of this gene family around 400 Mya, and indicated that genome segmental duplication was very likely the primary mechanism underlying the expansion of the PPR gene family in vascular plants. An analysis of a complete set of SiPPR genes/proteins that included classification, chromosomal location, orthologous relationships, duplication analysis, and auxiliary motifs is presented. Expression analysis of the SiPPR genes under stress conditions revealed that the expression of 24 SiPPR genes was responsive to abiotic stress. Subcellular localization analysis of 11 PPR proteins indicated that 5 proteins were localized to chloroplasts, that 4 were localized to mitochondria, and that 2 were localized to the cytoplasm. Conclusions: Our results contribute to a more comprehensive understanding the roles of PPR proteins and will be useful in the prioritization of particular PPR proteins for subsequent functional validation studies in foxtail millet. [ABSTRACT FROM AUTHOR]
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- 2016
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10. Genetic diversity and population genetics of the warble flies Hypoderma bovis and H. sinense in Qinghai Province, China.
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Yong Fu, Wei Li, Hong Duo, Zhi-Hong Guo, Ying Li, and Yan-Ming Zhang
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DIPTERA ,OESTRIDAE ,CATTLE parasites ,PATHOGENIC fungi ,PATHOGENIC bacteria ,GENETIC polymorphisms - Abstract
Background: Hypoderma bovis and H. sinense (Diptera: Oestridae) mainly parasitise cattle and yaks. The two parasites are pathogenic and cause economic losses that result from reduced amounts of livestock products, including milk, meat, and skin. Genetic diversity and population genetic structure of H. bovis and H. sinense have not been evaluated, but could be used to inform appropriate strategies to control these parasites. Methods: We cloned and sequenced part of the mitochondrial cytochrome c oxidase subunit I (COI) gene from 60 H. bovis isolates and 52 H. sinense isolates from five locations in Qinghai Province, China, to identify polymorphisms, and infer their phylogenetic relationships, historical population expansions, and divergence time. Results: We identified 17 COI haplotypes from the H. bovis samples, and 23 COI haplotypes from the H. sinense samples. The haplotype and nucleotide diversities were 0.738 and 0.00202 for H. bovis, and 0.867 and 0.00300 for H. sinense, respectively, which indicates rich genetic diversity in H. bovis and H. sinense populations. Bayesian phylogenetic analysis revealed that the two species are monophyletic, and geographical structuring of haplotypes was significantly different in H. sinense (P < 0.05), but not H. bovis. Neutrality tests and mismatch distribution statistical analysis revealed that populations of the two species have undergone demographic expansions. The divergence three Hypoderma spp. (H. bovis, H. lineatum, and H. sinense) was estimated to have occurred approximately 4.5 million years ago (Mya), which indicates that the rapid uplift of the Qinghai-Tibetan Plateau during the late Miocene-Pliocene was associated with divergence of Hypoderma species. Conclusions: Results of the present study revealed that both H. bovis and H. sinense displayed high genetic diversity and widespread population genetic differentiation within and among populations; these data, along with the molecular phylogeny, demographic history, and divergence time estimation, provide new insight into evolutionary history of these species. These findings will help elucidate speciation in Hypoderma and provide theoretical basis for epidemiological surveillance and control of these species on the Qinghai-Tibetan Plateau. [ABSTRACT FROM AUTHOR]
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- 2016
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11. Diagnosis and molecular characterization of rabies virus from a buffalo in China: a case report
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Huanchun Chen, Keshan Zhang, Jian-hong Guo, Bin Wu, Zhuofei Xu, and Min Xiang
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China ,Buffaloes ,Rabies ,Molecular Sequence Data ,Sequence alignment ,Case Report ,Biology ,medicine.disease_cause ,DNA sequencing ,law.invention ,lcsh:Infectious and parasitic diseases ,Animal Diseases ,Mice ,Viral Proteins ,law ,Phylogenetics ,Virology ,medicine ,Animals ,Humans ,lcsh:RC109-216 ,Amino Acid Sequence ,Peptide sequence ,Polymerase chain reaction ,Phylogeny ,Glycoproteins ,Phylogenetic tree ,Rabies virus ,medicine.disease ,Infectious Diseases ,Cattle ,Sequence Alignment - Abstract
Background Rabies virus (RABV) can infect many different species of warm-blooded animals. Glycoprotein G plays a key role in viral pathogenicity and neurotropism, and includes antigenic domains that are responsible for membrane fusion and host cell receptor recognition. Case presentation A case of buffalo rabies in China was diagnosed by direct fluorescent antibody test, G gene reverse-transcriptase polymerase chain reaction, and RABV mouse inoculation test. Molecular characterization of the RABV was performed using DNA sequencing, phylogenetic analysis and amino acid sequence comparison based on the G gene from different species of animals. Conclusion The results confirmed that the buffalo with suspected rabies was infected by RABV, which was genetically closely related to HNC (FJ602451) that was isolated from cattle in China in 2007. Comparison of the G gene among different species of animal showed that there were almost no amino acid changes among RABVs isolated from the same species of animals that distributed in a near region. However, there were many changes among RABVs that were isolated from different species of animal, or the same species from different geographic regions. This is believed to be the first case report of buffalo rabies in China, and the results may provide further information to understand the mechanism by which RABV breaks through the species barrier.
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- 2011
12. Neuroprotective effects of Eucommia ulmoides Oliv. and its bioactive constituent work via ameliorating the ubiquitin-proteasome system.
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Hong Guo, Fang Shi, Meijiao Li, Qingqing Liu, Bin Yu, and Limin Hu
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DRUG therapy for Parkinson's disease ,ANIMAL behavior ,ANIMAL experimentation ,BIOLOGICAL assay ,BIOPHYSICS ,CELL culture ,DOPAMINE ,HIGH performance liquid chromatography ,RESEARCH methodology ,MICE ,MOLECULAR structure ,NEUROLOGIC manifestations of general diseases ,PARKINSON'S disease ,PYRIDINE ,RESEARCH funding ,PLANT extracts ,NEUROPROTECTIVE agents ,DATA analysis software ,DESCRIPTIVE statistics ,IN vitro studies - Abstract
Background: Parkinson's disease (PD) is a chronic neurodegenerative disorder characterized by a loss of dopaminergic neurons in the substantia nigra, decreased striatal dopamine levels, and consequent extrapyramidal motor dysfunction. The purpose of this study was to investigate potential in vivo protective effects of Duzhong against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), as well as the bioactive constituents against 1-methyl-4-phenylpyridinium (MPP
+ ) toxicity in vitro. Methods: Male C57BL/6 mice were intraperitoneally administrated five consecutive injections of MPTP every 24 h at a dose of 30 mg/kg to induce an in vivo PD model. Pole and traction tests were performed in mice to evaluate motor deficits and bradykinesia after the final MPTP administration. The striatal levels of dopamine and its metabolites, 3,4-dihydroxyphenylacetic acid and homovanilic acid, were measured using a High-performance liquid chromatography-electrical conductivity detector. To further explore the bioactive constituents and protective mechanisms of Duzhong, seven compounds from Duzhong were tested on MPP+ -treated SH-SY5Y cell lines in vitro. A proteasome enzymatic assay and Cell Counting Kit-8 were performed to examine proteasomal activity and cell viability of Duzhong-treated cells, respectively, after exposure to MPP+ and proteasome inhibitor MG132. Results: Duzhong antagonized the loss of striatal neurotransmitters and relieved the associated anomaly in ambulatory locomotor activity in PD mice after a 3-day pre-treatment of Duzhong crude extract. The five Duzhong compounds attenuated MPP+ -induced dysfunction of protease activity and reduced MG132-induced cytotoxicity. Conclusion: Duzhong could serve as a potential candidate for PD treatment, and its mechanism involves the amelioration of the ubiquitin-proteasome system. [ABSTRACT FROM AUTHOR]- Published
- 2015
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13. Three cases of sporadic meningioangiomatosis with different imaging appearances: case report and review of the literature.
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Zhihua Sun, Fei Jin, Jing Zhang, Yue Fu, Wei Li, Hong Guo, and Yunting Zhang
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MENINGIOMA ,ANGIOMATOSIS ,TISSUE wounds ,SCREEN-film radiography ,MAGNETIC resonance imaging ,COMPUTED tomography - Abstract
Background: Meningioangiomatosis (MA) is a rare meningiovascular malformation or hamartomatous lesion in the central nervous system. Radiographic findings of MA may show a variety of characteristics according to different histological components. We present three cases of sporadic MA with different imaging appearances in an attempt to identify specific imaging characteristics. Case presentation: In case 1, an irregular hyperdense solid mass was localized in the left middle cranial fossa, demonstrating low and equal signal intensity on T1-weighted imaging (T1WI; TR/TE 2,048.9 ms/26.1 ms), high signal intensity with multiple flow void effect on T2-weighted imaging (T2WI; TR/TE 4,000 ms/106.4 ms), and significant and homogeneous enhancement on post-contrast magnetic resonance imaging (MRI). In case 2, the lesion in the right insular lobe showed a cystic-mural nodule pattern. The cystic content demonstrated similar density or signal intensity as cerebrospinal fluid, while the mural nodule demonstrated equal density or signal intensity on computed tomography (CT) and MRI. On post-contrast MRI, the mural nodule showed significant enhancement, but the cystic wall and content showed no enhancement. In case 3, a remarkably enhanced solid nodule was found in the cortex of the left parietal lobe with multiple small cysts surrounding it. This nodule showed low signal intensity on T2WI and diffusion-weighted imaging (DWI; TR/TE 6,000 ms/96.8 ms, b = 1,000 s/mm
2 ). The preoperative diagnoses of the above three cases were meningioma, hemangioblastoma, and ganglioglioma. However, all were pathologically diagnosed as MA. Conclusion: The presented cases demonstrate that MA may present with solid and cystic imaging patterns, which may include large cystic-mural nodules and small intra- and extra-cystic patterns. Although MA imaging diagnoses are difficult, several MRI signs may include specific characteristics, such as a flow void effect on T2WI and separating cysts in the cystic MA (as shown in our cases), gyriform hyperintensity on T2-fluid attenuated inversion recovery (FLAIR) sequence, and susceptibility artifacts on T2 gradient echo (GRE) sequences (as found in the literature). [ABSTRACT FROM AUTHOR]- Published
- 2015
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14. Differential proteomic analysis of grapevine leaves by iTRAQ reveals responses to heat stress and subsequent recovery.
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Guo-Tian Liu, Ling Ma, Wei Duan, Bai-Chen Wang, Ji-Hu Li, Hong-Guo Xu, Xue-Qing Yan, Bo-Fang Yan, Shao-Hua Li, and Li-Jun Wang
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PROTEOMICS ,GRAPES ,PHYSIOLOGICAL effects of heat ,TOLERATION ,PHOTOSYNTHESIS - Abstract
Background High temperature is a major environmental factor limiting grape yield and affecting berry quality. Thermotolerance includes the direct response to heat stress and the ability to recover from heat stress. To better understand the mechanism of the thermotolerance of Vitis, we combined a physiological analysis with iTRAQ-based proteomics of Vitis vinifera cv Cabernet Sauvignon, subjected to 43°C for 6 h, and then followed by recovery at 25/18°C. Results High temperature increased the concentrations of TBARS and inhibited electronic transport in photosynthesis apparatus, indicating that grape leaves were damaged by heat stress. However, these physiological changes rapidly returned to control levels during the subsequent recovery phase from heat stress. One hundred and seventy-four proteins were differentially expressed under heat stress and/or during the recovery phase, in comparison to unstressed controls, respectively. Stress and recovery conditions shared 42 proteins, while 113 and 103 proteins were respectively identified under heat stress and recovery conditions alone. Based on MapMan ontology, functional categories for these dysregulated proteins included mainly photosynthesis (about 20%), proteins (13%), and stress (8%). The subcellular localization using TargetP showed most proteins were located in the chloroplasts (34%), secretory pathways (8%) and mitochondrion (3%). Conclusion On the basis of these findings, we proposed that some proteins related to electron transport chain of photosynthesis, antioxidant enzymes, HSPs and other stress response proteins, and glycolysis may play key roles in enhancing grapevine adaptation to and recovery capacity from heat stress. These results provide a better understanding of the proteins involved in, and mechanisms of thermotolerance in grapevines. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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15. Genetic diversity of Plasmodium vivax population in Anhui province of China.
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Bo Huang, Shiguang Huang, Xin-zhuan Su, Hong Guo, Yucheng Xu, Fei Xu, Xuchu Hu, Yaming Yang, Shanqing Wang, and Fangli Lu
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PLASMODIUM vivax ,MALARIA ,BIOMARKERS ,GENETIC markers ,NUCLEOTIDE sequence - Abstract
Background Although the numbers of malaria cases in China have been declining in recent years, outbreaks of Plasmodium vivax malaria were still being reported in rural areas south of the Yellow River. To better understand the transmission dynamics of P. vivax parasites in China, the extent of genetic diversity of P. vivax populations circulating in Bozhou of Anhui province of China were investigated using three polymorphic genetic markers: merozoite surface proteins 1 and 3α (pvmsp-1 and pvmsp-3α) and circumsporozoite protein (pvcsp). Methods Forty-five P. vivax clinical isolates from Bouzhou of Anhui province were collected from 2009 to 2010 and were analysed using PCR/RFLP or DNA sequencing. Results Seven and six distinct allelic variants were identified using PCR/RFLP analysis of pvmsp-3α with HhaI and AluI, respectively. DNA sequence analysis of pvmsp-1 (variable block 5) revealed that there were Sal-I and recombinant types but not Belem type, and seven distinct allelic variants in pvmsp-1 were detected, with recombinant subtype 2 (R2) being predominant (66.7%). All the isolates carried pvcsp with VK210 type but not VK247 or P. vivax-like types in the samples. Sequence analysis of pvcsp gene revealed 12 distinct allelic variants, with VK210-1 being predominant (41.5%). Conclusions The present data indicate that there is some degree of genetic diversity among P. vivax populations in Anhui province of China. The genetic data obtained may assist in the surveillance of P. vivax infection in endemic areas or in tracking potential future disease outbreak. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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16. Upregulation of triglyceride synthesis in skeletal muscle overexpressing DGAT1.
- Author
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Feifei Yang, Zhuying Wei, Xiangbin Ding, Xinfeng Liu, Xiuguo Ge, Guimin Song, Guangpeng Li, and Hong Guo
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DIGLYCERIDES ,GENE expression ,LABORATORY mice ,TIBIAL arteries ,MYOCARDIUM - Abstract
The gene encoding diacylglycerol acyltransferase (DGAT1) is a functional and positional candidate gene for milk and intramuscular fat content. A bovine DGAT1 overexpression vector was constructed containing mouse MCK promoter and bovine DGAT1 cDNA. MCK-DGAT1 transgene in FVB mice was researched in present study. The transgene DGAT1 had a high level of expression in contrast to the endogenous DGAT1 in posterior tibial muscle of the transgenic mice, but a low expression level in the cardiac muscle. Compared with wild-type mice, triglyceride and DGAT1 content were approximately fourfold and 50% increased in posterior tibial muscle of the transgenic mice, respectively, while a little increase in cardiac muscle. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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17. Aquareovirus protein VP6 colocalizes with NS80 protein in infected and transfected cells.
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Dawei Wen, Liming Yan, Ling Shao, Hong Guo, Xiaoming Li, and Qin Fang
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CYTOSKELETAL proteins ,CAPSIDS ,ESCHERICHIA coli ,IMMUNOFLUORESCENCE ,CYTOPLASM ,IMMUNOBLOTTING - Abstract
Background: Aquareovirus particle is comprised of central core and outer capsid, which is built by seven structural proteins (VP1-VP7). The protein VP6 has been identified to be a clamp protein of stabilizing inner core frame VP3, and bridging outer shell protein VP5. However, the biological properties of VP6 in viral life cycle remain unknown. Results: The recombinant VP6 (rVP6) of aquareovirus was expressed in E. coli, and the polyclonal antibody against VP6 was generated by using purified rVP6 in this study. Following the preparation of VP6 antibody, the VP6 component in aquareovirus infected cells and purified viral particles was detected by Immunoblotting (IB) assay. Furthermore, using Immunofluorescence (IF) microscopy, singly transfected VP6 protein was observed to exhibit a diffuse distribution mainly in the cytoplasm, while it appeared inclusion phenotype in infected cells. Meanwhile, inclusion structures were also identified when VP6 was coexpressed with nonstructural protein NS80 in cotransfected cells. Conclusions: VP6 can be recruited by NS80 to its inclusions in both infected and transfected cells. The colocalization of VP6 and NS80 is corresponding to their homologous proteins σ2 and μNS in MRV. Our results suggest that VP6 may play a significant role in viral replication and particle assembly. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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18. Characterization and differential expression of microRNAs in the ovaries of pregnant and non-pregnant goats (Capra hircus).
- Author
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Zhang, Xiao-Dong, Zhang, Yun-Hai, Ling, Ying-Hui, Ya Liu, Cao, Hong-Guo, Yin, Zong-Jun, Ding, Jian-Ping, and Zhang, Xiao-Rong
- Subjects
MAMMAL reproduction ,GOATS ,PREGNANCY in animals ,GENE expression ,MICRORNA ,NUCLEOTIDE sequence ,OVARIAN follicle ,TRANSCRIPTION factors ,GENETIC regulation ,SECRETION - Abstract
Background: Ovarian follicular development and hormone secretion are complex and coordinated biological processes which will usually be altered during pregnancy. Ovarian function is tightly regulated by a multitude of genes, and also by some specific miRNAs. It is necessary to identify the differentially expressed miRNAs in the ovaries of pregnant and non-pregnant mammals, in order to further understand the role of miRNA-mediated posttranscriptional regulation in mammalian reproduction. Here, we performed a comprehensive search for hircine miRNAs using two small RNA sequencing libraries prepared from the ovaries of pregnant and non-pregnant goats. Results: 617 conserved and 7 putative novel miRNAs were identified in the hircine ovaries. A total of 471 conserved miRNAs (76.34%) were co-expressed in both pregnant and non-pregnant libraries, and 90 pregnancyspecific and 56 non-pregnancy-specific conserved miRNAs were identified. Additionally, 407 unique miRNAs (65.96%) were significantly differentially expressed in the pregnant and non-pregnant libraries, of which 294 were upregulated and 113 were downregulated in the pregnant library compared to the non-pregnant library. Further analysis showed that miR-143 was predicted to bind to the target sequences of Frizzled-6 and -3 receptor genes in the Wnt/beta-catenin signaling pathway, and let-7b may target the Activin receptor I and Smad 2/3 genes in the TGF-beta signaling pathway. The expression level of 5 randomly selected miRNAs were analyzed by quantitative real-time PCR (q-PCR), and the results demonstrated that the expression patterns were consistent with the Solexa sequencing results. Conclusions: The identification and characterization of differentially expressed miRNAs in the ovaries of pregnant and non-pregnant goats provides important information on the role of miRNA in the regulation of the ovarian development and function. This data will be helpful to facilitate studies on the regulation of miRNAs during mammalian reproduction. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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19. Transcriptomic analysis of grape (Vitis vinifera L.) leaves during and after recovery from heat stress.
- Author
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Guo-Tian Liu, Jun-Fang Wang, Cramer, Grant, Zhan-Wu Dai, Wei Duan, Hong-Guo Xu, Ben-Hong Wu, Pei-Ge Fan, Li-Jun Wang, and Shao-Hua Li
- Subjects
PHYSIOLOGICAL effects of heat ,ENZYMES ,METALLOENZYMES ,MICROBIAL genetics ,GENETICS - Abstract
Background: Grapes are a major fruit crop around the world. Heat stress can significantly reduce grape yield and quality. Changes at the molecular level in response to heat stress and subsequent recovery are poorly understood. To elucidate the effect of heat stress and subsequent recovery on expression of genes by grape leaves representing the classic heat stress response and thermotolerance mechanisms, transcript abundance of grape (Vitis vinifera L.) leaves was quantified using the Affymetrix Grape Genome oligonucleotide microarray (15,700 transcripts), followed by quantitative Real-Time PCR validation for some transcript profiles. Results: We found that about 8% of the total probe sets were responsive to heat stress and/or to subsequent recovery in grape leaves. The heat stress and recovery responses were characterized by different transcriptional changes. The number of heat stress-regulated genes was almost twice the number of recovery-regulated genes. The responsive genes identified in this study belong to a large number of important traits and biological pathways, including cell rescue (i.e., antioxidant enzymes), protein fate (i.e., HSPs), primary and secondary metabolism, transcription factors, signal transduction, and development. We have identified some common genes and heat shock factors (HSFs) that were modulated differentially by heat stress and recovery. Most HSP genes were upregulated by heat stress but were downregulated by the recovery. On the other hand, some specific HSP genes or HSFs were uniquely responsive to heat stress or recovery. Conclusion: The effect of heat stress and recovery on grape appears to be associated with multiple processes and mechanisms including stress-related genes, transcription factors, and metabolism. Heat stress and recovery elicited common up- or downregulated genes as well as unique sets of responsive genes. Moreover, some genes were regulated in opposite directions by heat stress and recovery. The results indicated HSPs, especially small HSPs, antioxidant enzymes (i.e., ascorbate peroxidase), and galactinol synthase may be important to thermotolerance of grape. HSF30 may be a key regulator for heat stress and recovery, while HSF7 and HSF1 may only be specific to recovery. The identification of heat stress or recovery responsive genes in this study provides novel insights into the molecular basis for heat tolerance in grape leaves. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
20. Cloning, expression and immunological evaluation of a short fragment from Rv3391 of Mycobacterium tuberculosis.
- Author
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Zhao, Jun-wei, Sun, Zhan-qiang, Yang, Hong-guo, Zhang, Chong-zhen, Yu, Xiao-li, Wen, Zi-lu, Gao, Yan-feng, Guo, Xiao-kui, Qi, Yuan-ming, and Zhang, Shu-lin
- Abstract
Tuberculosis (TB) is still one of the greatest health care problems in the world. In order to identify antigens that may be used in the serodiagnosis of active tuberculosis, a short fragment from Rv3391 ( fRv3391) of Mycobacterium tuberculosis was cloned and expressed. Its molecular weight and secondary structure elements were identified by mass spectrometry and circular dichroism. And its immunological nature was also evaluated by enzyme-linked immunosorbent assay (ELISA). The fRv3391 was expressed in Escherichia coli BL21 (DE3) with the molecular weight of 42.5 kDa and the secondary structure elements 36.2% α-helix, 0.0% β-sheet, 32.6% β-turn, and 31.3% random coil. Evaluation of fRv3391 as an ELISA solid-phase antigen on a set of human sera from well-characterized TB cases and healthy subjects revealed that there was strong serum antibody reactivity to fRv3391 in many human TB patients. Taken together, a short fragment from Rv3391 of Mycobacterium tuberculosis was cloned and expressed and the ELISA results showed that the protein may be useful as an immunodominant antigen for the serodiagnosis of active TB. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
21. Diagnosis and molecular characterization of rabies virus from a buffalo in China: a case report.
- Author
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Ke-shan Zhang, Jian-hong Guo, Zhuo-fei Xu, Min Xiang, Bin Wu, and Huan-chun Chen
- Subjects
- *
RABIES virus , *WATER buffalo , *RHABDOVIRUSES , *GLYCOPROTEINS , *AMINO acid sequence , *DISEASES - Abstract
Background: Rabies virus (RABV) can infect many different species of warm-blooded animals. Glycoprotein G plays a key role in viral pathogenicity and neurotropism, and includes antigenic domains that are responsible for membrane fusion and host cell receptor recognition. Case presentation: A case of buffalo rabies in China was diagnosed by direct fluorescent antibody test, G gene reverse-transcriptase polymerase chain reaction, and RABV mouse inoculation test. Molecular characterization of the RABV was performed using DNA sequencing, phylogenetic analysis and amino acid sequence comparison based on the G gene from different species of animals. Conclusion: The results confirmed that the buffalo with suspected rabies was infected by RABV, which was genetically closely related to HNC (FJ602451) that was isolated from cattle in China in 2007. Comparison of the G gene among different species of animal showed that there were almost no amino acid changes among RABVs isolated from the same species of animals that distributed in a near region. However, there were many changes among RABVs that were isolated from different species of animal, or the same species from different geographic regions. This is believed to be the first case report of buffalo rabies in China, and the results may provide further information to understand the mechanism by which RABV breaks through the species barrier. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
22. Bmi-1 promotes invasion and metastasis, and its elevated expression is correlated with an advanced stage of breast cancer.
- Author
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Bao-Hong Guo, Yan Feng, Rong Zhang, Li-Hua Xu, Man-Zhi Li, Hsiang-Fu Kung, Li-Bing Song, and Mu-Sheng Zeng
- Subjects
- *
LEUKEMIA , *ONCOGENES , *EPITHELIAL cells , *XENOGRAFTS , *METASTASIS - Abstract
Background: B-lymphoma Moloney murine leukemia virus insertion region-1 (Bmi-1) acts as an oncogene in various tumors, and its overexpression correlates with a poor outcome in several human cancers. Ectopic expression of Bmi-1 can induce epithelial-mesenchymal transition (EMT) and enhance the motility and invasiveness of human nasopharyngeal epithelial cells (NPECs), whereas silencing endogenous Bmi-1 expression can reverse EMT and reduce the metastatic potential of nasopharyngeal cancer cells (NPCs). Mouse xenograft studies indicate that coexpression of Bmi-1 and H-Ras in breast cancer cells can induce an aggressive and metastatic phenotype with an unusual occurrence of brain metastasis; although, Bmi-1 overexpression did not result in oncogenic transformation of MCF-10A cells. However, the underlying molecular mechanism of Bmi-1-mediated progression and the metastasis of breast cancer are not fully elucidated at this time. Results: Bmi-1 expression is more pronouncedly increased in primary cancer tissues compared to matched adjacent non-cancerous tissues. High Bmi-1 expression is correlated with advanced clinicopathologic classifications (T, N, and M) and clinical stages. Furthermore, a high level of Bmi-1 indicates an unfavorable overall survival and serves as a high risk marker for breast cancer. In addition, inverse transcriptional expression levels of Bmi-1 and E-cadherin are detected between the primary cancer tissues and the matched adjacent non-cancerous tissues. Higher Bmi-1 levels are found in the cancer tissue, whereas the paired adjacent non-cancer tissue shows higher E-cadherin levels. Overexpression of Bmi-1 increases the motility and invasive properties of immortalized human mammary epithelial cells, which is concurrent with the increased expression of mesenchymal markers, the decreased expression of epithelial markers, the stabilization of Snail and the dysregulation of the Akt/GSK3β pathway. Consistent with these observations, the repression of Bmi-1 in highly metastatic breast cancer cells remarkably reduces cellular motility, invasion and transformation, as well as tumorigenesis and lung metastases in nude mice. In addition, the repression of Bmi-1 reverses the expression of EMT markers and inhibits the Akt/GSK3β/Snail pathway. Conclusions: This study demonstrates that Bmi-1 promotes the invasion and metastasis of human breast cancer and predicts poor survival. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
23. An atypical 7Q11.2-Q21.11 deletion in a Williams- Beuren syndome patient.
- Author
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Wei Lu, Yi Yang, Xiao-hong Guo, Lin Yang, Hui-jun Wang, Ren-chao Liu, Yu An, and Fei-hong Luo
- Subjects
WILLIAMS syndrome ,SYNDROMES in children - Abstract
An abstract of the study "An Atypical 7Q11.2-Q21.11 Deletion in a Williams-Beuren Syndrome Patient" by Wei Lu et al is presented.
- Published
- 2013
- Full Text
- View/download PDF
24. Molecular characterization of subcellular localization and nucleocytoplasmic shuttling of PRV UL54.
- Author
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Meili Li, Shuai Wang, Junji Xing, Hong Guo, and Chunfu Zheng
- Subjects
AUJESZKY'S disease virus - Abstract
An abstract of the paper "Molecular characterization of subcellular localization and nucleocytoplasmic shuttling of PRV UL54," by Shuai Wang and colleagues is presented.
- Published
- 2011
- Full Text
- View/download PDF
25. Screening and identification of host cellular factors interaction with immediate-early protein ICP22 of herpes simplex virus type 1.
- Author
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Junji Xing, Fusen Lin, Meili Li, Shuai Wang, Hong Guo, and Chunfu Zheng
- Subjects
HERPES simplex - Abstract
An abstract of the paper on the identification of host cellular factors interaction with protein ICP22 of herpes simples virus type 1 presented during the Institut Pasteur International Network Annual Scientific Meeting at Hong Kong, China, from November 22-23, 2010 is presented.
- Published
- 2011
- Full Text
- View/download PDF
26. Differential proteomic analysis of grapevine leaves by iTRAQ reveals responses to heat stress and subsequent recovery.
- Author
-
Liu GT, Ma L, Duan W, Wang BC, Li JH, Xu HG, Yan XQ, Yan BF, Li SH, and Wang LJ
- Subjects
- Cell Membrane metabolism, Electron Transport, Gene Ontology, Photosynthesis, Photosystem II Protein Complex metabolism, Plant Proteins classification, Plant Proteins metabolism, Protein Transport, Sequence Homology, Amino Acid, Subcellular Fractions metabolism, Temperature, Thiobarbituric Acid Reactive Substances metabolism, Vitis metabolism, Heat-Shock Response, Isotope Labeling methods, Plant Leaves metabolism, Proteomics methods, Stress, Physiological, Vitis physiology
- Abstract
Background: High temperature is a major environmental factor limiting grape yield and affecting berry quality. Thermotolerance includes the direct response to heat stress and the ability to recover from heat stress. To better understand the mechanism of the thermotolerance of Vitis, we combined a physiological analysis with iTRAQ-based proteomics of Vitis vinifera cv Cabernet Sauvignon, subjected to 43°C for 6 h, and then followed by recovery at 25/18°C., Results: High temperature increased the concentrations of TBARS and inhibited electronic transport in photosynthesis apparatus, indicating that grape leaves were damaged by heat stress. However, these physiological changes rapidly returned to control levels during the subsequent recovery phase from heat stress. One hundred and seventy-four proteins were differentially expressed under heat stress and/or during the recovery phase, in comparison to unstressed controls, respectively. Stress and recovery conditions shared 42 proteins, while 113 and 103 proteins were respectively identified under heat stress and recovery conditions alone. Based on MapMan ontology, functional categories for these dysregulated proteins included mainly photosynthesis (about 20%), proteins (13%), and stress (8%). The subcellular localization using TargetP showed most proteins were located in the chloroplasts (34%), secretory pathways (8%) and mitochondrion (3%)., Conclusion: On the basis of these findings, we proposed that some proteins related to electron transport chain of photosynthesis, antioxidant enzymes, HSPs and other stress response proteins, and glycolysis may play key roles in enhancing grapevine adaptation to and recovery capacity from heat stress. These results provide a better understanding of the proteins involved in, and mechanisms of thermotolerance in grapevines.
- Published
- 2014
- Full Text
- View/download PDF
27. Transcriptomic analysis of grape (Vitis vinifera L.) leaves during and after recovery from heat stress.
- Author
-
Liu GT, Wang JF, Cramer G, Dai ZW, Duan W, Xu HG, Wu BH, Fan PG, Wang LJ, and Li SH
- Subjects
- Cluster Analysis, DNA Probes metabolism, Down-Regulation genetics, Genes, Plant genetics, Oligonucleotide Array Sequence Analysis, RNA, Messenger genetics, RNA, Messenger metabolism, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, Up-Regulation genetics, Gene Expression Profiling, Gene Expression Regulation, Plant, Heat-Shock Response genetics, Plant Leaves genetics, Vitis genetics
- Abstract
Background: Grapes are a major fruit crop around the world. Heat stress can significantly reduce grape yield and quality. Changes at the molecular level in response to heat stress and subsequent recovery are poorly understood. To elucidate the effect of heat stress and subsequent recovery on expression of genes by grape leaves representing the classic heat stress response and thermotolerance mechanisms, transcript abundance of grape (Vitis vinifera L.) leaves was quantified using the Affymetrix Grape Genome oligonucleotide microarray (15,700 transcripts), followed by quantitative Real-Time PCR validation for some transcript profiles., Results: We found that about 8% of the total probe sets were responsive to heat stress and/or to subsequent recovery in grape leaves. The heat stress and recovery responses were characterized by different transcriptional changes. The number of heat stress-regulated genes was almost twice the number of recovery-regulated genes. The responsive genes identified in this study belong to a large number of important traits and biological pathways, including cell rescue (i.e., antioxidant enzymes), protein fate (i.e., HSPs), primary and secondary metabolism, transcription factors, signal transduction, and development. We have identified some common genes and heat shock factors (HSFs) that were modulated differentially by heat stress and recovery. Most HSP genes were upregulated by heat stress but were downregulated by the recovery. On the other hand, some specific HSP genes or HSFs were uniquely responsive to heat stress or recovery., Conclusion: The effect of heat stress and recovery on grape appears to be associated with multiple processes and mechanisms including stress-related genes, transcription factors, and metabolism. Heat stress and recovery elicited common up- or downregulated genes as well as unique sets of responsive genes. Moreover, some genes were regulated in opposite directions by heat stress and recovery. The results indicated HSPs, especially small HSPs, antioxidant enzymes (i.e., ascorbate peroxidase), and galactinol synthase may be important to thermotolerance of grape. HSF30 may be a key regulator for heat stress and recovery, while HSF7 and HSF1 may only be specific to recovery. The identification of heat stress or recovery responsive genes in this study provides novel insights into the molecular basis for heat tolerance in grape leaves.
- Published
- 2012
- Full Text
- View/download PDF
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