1. A mitochondria-targeted ratiometric NIR fluorescent probe for simultaneously monitoring viscosity and ONOO− based on two different channels in living HepG2 cells.
- Author
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Liu, Qingqing, Dong, Chao, Zhang, Jing, Zhao, Bo, Zhou, Yongqing, Fan, Chunhua, and Lu, Zhengliang
- Subjects
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FLUORESCENT probes , *INTRAMOLECULAR charge transfer , *VISCOSITY , *CELL imaging - Abstract
Mitochondrial viscosity and ONOO− are involved in various pathological and physiological processes. Abnormal viscosity or irregular ONOO− concentration would disrupt the normal function of cells. Hence, we skillfully designed and synthesized a dual-responsive probe Mito-HC-TZ for the simultaneous detection of ONOO− and viscosity by different channels. 3-ethylbenzothiazolium was acted as fluorescent rotor to regulate the twisted intramolecular charge transfer (TICT) process; the C = C of 3-ethylbenzothiazolium linked to the xanthene core could be easily broken by ONOO−. Thus, the probe responded to viscosity and ONOO− by red and orange fluorescence channels, respectively. Mito-HC-TZ had high selectivity, low cytotoxicity and wide range of pH values. The results of cell imaging experiments showed the probe could be used to respectively monitor the viscosity and ONOO− level in HepG2 cells. [Display omitted] • A NIR probe for viscosity and ONOO− according to two channels was developed. • Mito-HC-TZ could ratiometrically respond to mitochondrial viscosity and ONOO−. • Mito-HC-TZ was applied to detect the changes of viscosity and ONOO− in cells. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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