1. The protective effects of total saponins from Ornithogalum saundersiae (Liliaceae) on acute hepatic failure induced by lipopolysaccharide and d-galactosamine in mice
- Author
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Ying-Wan, Wu, Yan-Ling, Feng, Xiao-Chun, Lian, Li-Hua, Jiang, Ying-Zi, and Nan, Ji-Xing
- Subjects
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SAPONINS , *LILIACEAE , *LIVER failure , *ENDOTOXINS , *GALACTOSE , *LABORATORY mice , *ENZYME-linked immunosorbent assay , *AMINOTRANSFERASES , *MEDICINAL plants , *ALTERNATIVE medicine , *ANALYSIS of variance , *ANIMAL experimentation , *BIOPHYSICS , *COMPUTER software , *HISTOLOGICAL techniques , *IMMUNOHISTOCHEMISTRY , *LIVER , *RESEARCH methodology , *MICE , *RESEARCH funding , *STATISTICS , *WESTERN immunoblotting , *PHYTOCHEMICALS , *PLANT extracts , *DATA analysis , *PREVENTION - Abstract
Ethnopharmacological relevance: This study examined the protective effects of total saponins from Ornithogalum saundersiae (Liliaceae) on d-galactosamine (d-GalN) and lipopolysaccharide (LPS) – induced fulminant hepatic failure. Materials and methods: Total saponins of Ornithogalum saundersiae (Liliaceae) (OC) were prepared with ethyl alcohol extract from bulbs of the plant. Mice were given an intraperitoneal injection of d-GalN (700mg/kg)/LPS (10μg/kg). OC (100mg/kg, 200mg/kg and 300mg/kg) was administered orally for 3 days continuously, and at the last day at 1h before the d-GalN/LPS injection. Mice were sacrificed at 8h after the d-GalN/LPS injection. The liver injury was assessed biochemically, investigating aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA), glutathione (GSH) activities, and the expressions of caspase-3 and hypoxia inducible factor-1α (HIF-1α) as well. Tumor necrosis factor (TNF-α) content was measured after d-GalN/LPS induced 1h by ELISA assay. The survival rates after application of OC in 24h also were observed. Results: d-GalN/LPS increased the serum aminotransferase levels and lipid peroxidation, while decreased the reduced glutathione level. The pretreatment with OC attenuated these changes in a dose-dependent manner. Elevation of TNF-α level and activation of caspase-3, HIF-1α were observed in the d-GalN/LPS group, which was attenuated by OC. The survival rate of the OC groups was significantly higher than that of the d-GalN/LPS group. Conclusions: Protection afforded by OC against d-GalN/LPS-induced fulminant hepatic failure is the result of reduced oxidative stress, inhibited expression of caspase-3, HIF-1α, and anti-apoptotic activity. [Copyright &y& Elsevier]
- Published
- 2010
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